breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 67,887:1 | +G | 5.7% | coding (451/1503 nt) | araA ← | L‑arabinose isomerase |
RA | 67,887:2 | +T | 5.7% | coding (451/1503 nt) | araA ← | L‑arabinose isomerase |
MC JC | 257,908 | Δ776 bp | 100% | [crl] | [crl] | |
JC JC | 1,293,032 | IS1 (–) +8 bp | 100% | intergenic (‑110/‑488) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
MC JC | 1,299,499 | Δ1,199 bp | 100% | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
RA | 1,311,197 | G→T | 16.2% | E37* (GAA→TAA) | tonB → | membrane spanning protein in TonB‑ExbB‑ExbD transport complex |
RA | 1,466,582 | T→C | 9.3% | pseudogene (1191/2513 nt) | ydbA → | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
RA | 1,466,588 | C→T | 6.0% | pseudogene (1197/2513 nt) | ydbA → | pseudogene, autotransporter homolog; interrupted by IS2 and IS30 |
RA | 1,708,930 | C→A | 9.0% | P671Q (CCG→CAG) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
MC JC | 1,978,503 | Δ776 bp | 100% | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | 100% | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
JC | 2,023,796 | Δ5 bp | 14.5% | intergenic (+118/‑168) | fliR → / → rcsA | flagellar export pore protein/transcriptional regulator of colanic acid capsular biosynthesis |
RA | 2,132,787 | A→C | 100% | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,173,361 | Δ2 bp | 100% | pseudogene (1‑2/442 nt) | gatC ← | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,316,394 | A→G | 39.0% | Y73C (TAC→TGC) | rcsB → | response regulator in two‑component regulatory system with RcsC and YojN |
RA | 2,316,681 | A→G | 53.9% | T169A (ACC→GCC) | rcsB → | response regulator in two‑component regulatory system with RcsC and YojN |
RA | 3,527,591 | C→T | 61.2% | Q375* (CAG→TAG) | yrfF → | putative RcsCDB‑response attenuator, inner membrane protein |
RA | 3,560,455:1 | +G | 100% | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
MC JC | 3,815,859 | Δ82 bp | 100% | [rph]–[rph] | [rph], [rph] | |
RA | 3,992,438 | T→G | 38.6% | L429* (TTA→TGA) | cyaA → | adenylate cyclase |
JC JC | 3,992,705 | IS5 (+) +4 bp | 58.3% | coding (1553‑1556/2547 nt) | cyaA → | adenylate cyclase |
JC | 4,001,645 | Δ5 bp | 100% | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,543 | C→A | 100% | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,190 | A→G | 28.9% | intergenic (+396/+246) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,191 | A→C | 41.6% | intergenic (+397/+245) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,268 | T→C | 24.5% | intergenic (+474/+168) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,286 | C→T | 12.5% | intergenic (+492/+150) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,296,380:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 2533675 | 2536437 | 2763 | 75 [74] | [74] 77 | ptsH–[pdxK] | ptsH,ptsI,crr,[pdxK] |
* | * | ÷ | NC_000913 | 3423803–3424416 | 3424416 | 1–614 | 75 [74] | [74] 75 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 307356 = | 123 (0.830) | 6 (0.040) | 5/482 | NT | 5.0% | coding (1977/2526 nt) | ecpC | ECP production outer membrane protein |
? | NC_000913 | 307385 = | 108 (0.760) | coding (1948/2526 nt) | ecpC | ECP production outer membrane protein | |||||
* | ? | NC_000913 | 1207790 = | 80 (0.540) | 87 (0.630) | 65/466 | NT | 54.9% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 68 (0.490) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 78 (0.530) | 73 (0.530) | 55/466 | NT | 50.9% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 68 (0.490) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | 3845531 = | 109 (0.740) | 8 (0.060) | 8/478 | NT | 8.3% | coding (1568/1767 nt) | adeD | cryptic adenine deaminase |
? | NC_000913 | = 3992760 | 73 (0.520) | coding (1608/2547 nt) | cyaA | adenylate cyclase |