breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 57,295 G→A 3.1% intergenic (‑186/‑69) lptD ← / → djlA LPS assembly OM complex LptDE, beta‑barrel component/membrane‑anchored DnaK co‑chaperone, DNA‑binding protein
RA 210,489 A→G 3.2% I271V (ATT→GTT)  ldcC → lysine decarboxylase 2, constitutive
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 349,717 T→C 4.9% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 357,510 C→A 2.6% intergenic (+56/+281) codA → / ← cynR cytosine/isoguanine deaminase/transcriptional activator of cyn operon, autorepressor
RA 375,247 A→G 3.6% intergenic (+366/‑212) mhpE → / → mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
RA 475,054 G→A 2.9% G36G (GGC→GGT tesB ← acyl‑CoA thioesterase 2
RA 738,514 A→G 2.7% G141G (GGA→GGG ybfD → H repeat‑associated putative transposase
RA 847,213 A→T 2.7% intergenic (‑94/+45) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit
RA 847,215 A→T 2.8% intergenic (‑96/+43) glnP ← / ← glnH glutamine transporter subunit/glutamine transporter subunit
RA 852,177 A→C 4.7% F141V (TTC→GTC)  opgE ← OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 884,387 A→G 6.1% I239V (ATC→GTC)  mdfA → multidrug efflux system protein
RA 1,269,451 G→T 3.5% intergenic (+62/+252) rdlA → / ← ldrB sRNA antisense regulator affects LdrA translation, proposed addiction module in LDR‑A repeat, with toxic peptide LdrA/toxic polypeptide, small
RA 1,424,488 T→A 2.5% F236Y (TTT→TAT)  trkG → Rac prophage, potassium transporter subunit
RA 1,552,023 T→C 2.7% intergenic (+32/+375) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
RA 1,708,766 C→T 6.7% V616V (GTC→GTT rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,844 C→G 5.8% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,830,060 G→C 2.9% R236G (CGC→GGC)  astA ← arginine succinyltransferase
RA 1,830,064 A→C 3.1% P234P (CCT→CCG) ‡ astA ← arginine succinyltransferase
RA 1,830,065 G→C 2.7% P234R (CCT→CGT) ‡ astA ← arginine succinyltransferase
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,024,254 Δ1 bp 4.9% coding (287/624 nt) rcsA → transcriptional regulator of colanic acid capsular biosynthesis
RA 2,066,465 T→C 100% K286R (AAG→AGG)  insH1 ← IS5 transposase and trans‑activator
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,253,008 Δ1 bp 2.6% coding (1136/1251 nt) nupX ← nucleoside permease
RA 2,275,838 T→G 3.9% F190V (TTT→GTT) ‡ yejE → microcin C ABC transporter permease
RA 2,275,839 T→A 3.2% F190Y (TTT→TAT) ‡ yejE → microcin C ABC transporter permease
RA 2,275,840 T→A 3.3% F190L (TTT→TTA) ‡ yejE → microcin C ABC transporter permease
RA 2,275,841 T→A 3.2% L191M (TTG→ATG) ‡ yejE → microcin C ABC transporter permease
RA 2,275,842 T→G 3.2% L191W (TTG→TGG) ‡ yejE → microcin C ABC transporter permease
RA 2,275,843 G→T 3.0% L191F (TTG→TTT) ‡ yejE → microcin C ABC transporter permease
RA 2,275,845 T→A 3.2% I192N (ATT→AAT) ‡ yejE → microcin C ABC transporter permease
RA 2,275,846 T→A 3.5% I192I (ATT→ATA) ‡ yejE → microcin C ABC transporter permease
RA 2,275,849 T→A 3.2% I193I (ATT→ATA yejE → microcin C ABC transporter permease
RA 2,275,850 T→A 3.2% L194I (TTA→ATA) ‡ yejE → microcin C ABC transporter permease
RA 2,275,852 A→C 3.7% L194F (TTA→TTC) ‡ yejE → microcin C ABC transporter permease
RA 2,275,853 C→A 3.3% L195I (CTT→ATT) ‡ yejE → microcin C ABC transporter permease
RA 2,275,854 T→A 3.1% L195H (CTT→CAT) ‡ yejE → microcin C ABC transporter permease
RA 2,275,855 T→A 3.1% L195L (CTT→CTA) ‡ yejE → microcin C ABC transporter permease
RA 2,275,856 T→A 3.7% S196T (TCC→ACC) ‡ yejE → microcin C ABC transporter permease
RA 2,275,857 C→A 3.2% S196Y (TCC→TAC) ‡ yejE → microcin C ABC transporter permease
RA 2,304,678 T→C 5.8% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,011 A→G 3.9% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,368,587 Δ1 bp 2.7% coding (551/1983 nt) arnA → fused UDP‑L‑Ara4N formyltransferase/UDP‑GlcA C‑4'‑decarboxylase
RA 2,387,336 Δ1 bp 2.6% coding (405/504 nt) yfbM → DUF1877 family protein
RA 2,398,403 Δ1 bp 3.0% coding (1761/2727 nt) nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,442,533 A→G 3.3% N258D (AAC→GAC)  mnmC → fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,509,469 G→A 100% intergenic (‑45/‑159) glk ← / → yfeO glucokinase/putative ion channel protein
RA 2,547,379 T→C 2.8% W237R (TGG→CGG)  murP → N‑acetylmuramic acid permease, EIIBC component, PTS system
RA 2,748,668 G→A 2.8% V237I (GTC→ATC)  ypjD → cytochrome c assembly protein family inner membrane protein
RA 2,977,122 C→A 4.1% H176N (CAT→AAT)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
RA 2,977,560 Δ1 bp 52.2% coding (964/1032 nt) galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
RA 2,977,578 T→G 18.0% S328A (TCA→GCA)  galR → galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
RA 3,003,171 C→T 3.2% A187V (GCC→GTC)  xdhB → xanthine dehydrogenase, FAD‑binding subunit
RA 3,075,867 A→T 2.6% F105Y (TTC→TAC)  yggF ← fructose 1,6 bisphosphatase isozyme
RA 3,389,264 Δ1 bp 3.5% coding (72/204 nt) aaeX ← DUF1656 family putative inner membrane efflux pump associated protein
RA 3,528,106 T→G 3.5% L547R (CTG→CGG)  yrfF → putative RcsCDB‑response attenuator, inner membrane protein
RA 3,561,749 T→A 2.6% D25V (GAT→GTT)  glpE ← thiosulfate:cyanide sulfurtransferase (rhodanese)
RA 3,577,176 T→A 2.7% E139V (GAA→GTA)  gntK ← gluconate kinase 2
RA 3,589,827 C→G 3.2% G67A (GGC→GCC)  ugpE ← sn‑glycerol‑3‑phosphate ABC transporter permease
RA 3,810,323 G→A 2.5% intergenic (+20/+19) coaD → / ← mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,875,524 G→T 8.6% V30F (GTT→TTT)  yidX → putative lipoprotein
RA 3,875,537 C→A 7.5% A34E (GCA→GAA)  yidX → putative lipoprotein
RA 3,876,894 T→G 5.1% T20P (ACC→CCC)  yidA ← sugar phosphate phosphatase
RA 3,997,309:1 +A 2.5% coding (128/717 nt) yigB → 5‑amino‑6‑(5‑phospho‑D‑ribitylamino)uracil phosphatase, pyrimidine phosphatase, riboflavin synthesis
JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,295,833 T→C 9.2% intergenic (+40/+604) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,059 C→T 6.6% intergenic (+266/+378) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,154 T→C 12.4% intergenic (+361/+283) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,189 A→G 39.2% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 40.3% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,949 A→G 5.1% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,089 T→G 9.1% intergenic (‑347/+311) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,326,142 T→C 11.0% intergenic (‑400/+258) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,489,391 Δ1 bp 2.8% coding (674/1503 nt) yjgR ← DUF853 family protein with NTPase fold
RA 4,540,530 Δ1 bp 2.9% intergenic (‑1028/‑428) nanC ← / → fimB N‑acetylnuraminic acid outer membrane channel protein/tyrosine recombinase/inversion of on/off regulator of fimA
RA 4,593,336 Δ1 bp 3.4% intergenic (+23/+26) tsr → / ← lgoT methyl‑accepting chemotaxis protein I, serine sensor receptor/putative L‑galactonate:H+ symporter
RA 4,593,339 A→T 3.4% intergenic (+26/+23) tsr → / ← lgoT methyl‑accepting chemotaxis protein I, serine sensor receptor/putative L‑galactonate:H+ symporter
RA 4,593,341 A→T 3.4% intergenic (+28/+21) tsr → / ← lgoT methyl‑accepting chemotaxis protein I, serine sensor receptor/putative L‑galactonate:H+ symporter
RA 4,593,343:1 +C 3.4% intergenic (+30/+19) tsr → / ← lgoT methyl‑accepting chemotaxis protein I, serine sensor receptor/putative L‑galactonate:H+ symporter
RA 4,621,614 C→T 2.5% intergenic (+11/‑156) deoD → / → yjjJ purine nucleoside phosphorylase 1, nicotinamide 1‑beta‑D‑riboside phosphorylase/putative protein kinase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2533708 2536446 2739 82 [81] [77] 86 ptsH–[pdxK] ptsH, ptsI, crr, [pdxK]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)176 (1.040) 99/510 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 = 381689NA (NA)15 (0.090) 14/492 NT NA coding (339/366 nt)
coding (16/906 nt)
insC1
insD1
IS2 repressor TnpA
IS2 transposase TnpB
?NC_000913 = 381743 NA (NA)coding (70/906 nt) insD1 IS2 transposase TnpB
* ? NC_000913 847204 =132 (0.780)4 (0.020) 4/492 NT 3.1% intergenic (‑85/+54) glnP/glnH glutamine transporter subunit/glutamine transporter subunit
?NC_000913 847225 = 122 (0.750)intergenic (‑106/+33) glnP/glnH glutamine transporter subunit/glutamine transporter subunit
* ? NC_000913 = 907278149 (0.880)4 (0.020) 4/496 NT 2.9% coding (1005/1431 nt) ybjT putative NAD‑dependent oxidoreductase
?NC_000913 = 907329 124 (0.750)coding (954/1431 nt) ybjT putative NAD‑dependent oxidoreductase
* ? NC_000913 1207790 =65 (0.380)75 (0.470) 54/478 NT 51.0% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 83 (0.520)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780561 (0.360)56 (0.350) 45/478 NT 44.4% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 83 (0.520)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =3 (0.020)189 (1.120) 105/510 NT 98.4% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994981 (0.010)125 (0.750) 75/502 NT 99.6% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1830059125 (0.740)4 (0.020) 4/496 NT 3.1% coding (707/1035 nt) astA arginine succinyltransferase
?NC_000913 = 1830065 131 (0.800)coding (701/1035 nt) astA arginine succinyltransferase
* ? NC_000913 = 1946034134 (0.790)4 (0.020) 4/494 NT 3.1% intergenic (‑58/+81) ruvA/yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein
?NC_000913 = 1946036 124 (0.760)intergenic (‑60/+79) ruvA/yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein
* ? NC_000913 1979486 =0 (0.000)120 (0.710) 69/510 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)137 (0.810) 79/510 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 2275830 =173 (1.020)8 (0.050) 8/494 NT 4.6% coding (560/1026 nt) yejE microcin C ABC transporter permease
?NC_000913 2275866 = 162 (0.990)coding (596/1026 nt) yejE microcin C ABC transporter permease
* ? NC_000913 2288917 =NA (NA)142 (0.840) 88/510 NT 95.3% intergenic (‑5/+146) yejO/insH1 pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system/IS5 transposase and trans‑activator
?NC_000913 3526447 = 7 (0.040)intergenic (‑300/‑20) nudE/yrfF adenosine nucleotide hydrolase, Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein
* ? NC_000913 = 2290110NA (NA)190 (1.130) 92/508 NT 96.5% intergenic (‑31/+2) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
?NC_000913 = 3526451 7 (0.040)intergenic (‑304/‑16) nudE/yrfF adenosine nucleotide hydrolase, Ap3A/Ap2A/ADP‑ribose/NADH hydrolase/putative RcsCDB‑response attenuator, inner membrane protein
* ? NC_000913 = 2713827172 (1.020)6 (0.040) 6/488 NT 3.8% coding (934/1335 nt) srmB ATP‑dependent RNA helicase
?NC_000913 = 2713906 141 (0.870)coding (1013/1335 nt) srmB ATP‑dependent RNA helicase
* ? NC_000913 2796363 =137 (0.810)4 (0.020) 4/490 NT 2.9% coding (422/450 nt) ygaU uncharacterized protein
?NC_000913 2796384 = 136 (0.840)coding (401/450 nt) ygaU uncharacterized protein
* ? NC_000913 = 2972362141 (0.830)8 (0.050) 8/510 NT 5.3% coding (768/1041 nt) tas putative NADP(H)‑dependent aldo‑keto reductase
?NC_000913 2977089 = 145 (0.860)coding (493/1032 nt) galR galactose‑inducible d‑galactose regulon transcriptional repressor, autorepressor
* ? NC_000913 = 3220244159 (0.940)5 (0.030) 5/490 NT 3.2% coding (753/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
?NC_000913 = 3220396 149 (0.920)coding (905/1380 nt) patA putrescine:2‑oxoglutaric acid aminotransferase, PLP‑dependent
* ? NC_000913 3810311 =NA (NA)4 (0.020) 4/492 NT NA noncoding (1/22 nt) REP274 (repetitive extragenic palindromic) element, contains 1 REP sequences REP274 (repetitive extragenic palindromic) element, contains 1 REP sequences
?NC_000913 3810333 = NA (NA)intergenic (+30/+9) coaD/mutM pantetheine‑phosphate adenylyltransferase/formamidopyrimidine/5‑formyluracil/ 5‑hydroxymethyluracil DNA glycosylase
* ? NC_000913 3845530 =142 (0.840)134 (0.830) 80/490 NT 46.3% coding (1568/1767 nt) adeD cryptic adenine deaminase
?NC_000913 = 3992759 175 (1.080)coding (1608/2547 nt) cyaA adenylate cyclase
* ? NC_000913 4295831 =NA (NA)18 (0.130) 10/432 NT NA intergenic (+38/+606) gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
?NC_000913 = 4296395 NA (NA)noncoding (562/602 nt) RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites
* ? NC_000913 4593325 =NA (NA)5 (0.030) 5/488 NT NA intergenic (+12/+37) tsr/lgoT methyl‑accepting chemotaxis protein I, serine sensor receptor/putative L‑galactonate:H+ symporter
?NC_000913 4593355 = NA (NA)intergenic (+42/+7) tsr/lgoT methyl‑accepting chemotaxis protein I, serine sensor receptor/putative L‑galactonate:H+ symporter
* ? NC_000913 4614258 =NA (NA)4 (0.020) 4/490 NT NA noncoding (1/388 nt) REP352 (repetitive extragenic palindromic) element, contains 8 REP sequences REP352 (repetitive extragenic palindromic) element, contains 8 REP sequences
?NC_000913 = 4614661 NA (NA)intergenic (+400/+20) yjjV/yjjW putative DNase/putative pyruvate formate lyase activating enzyme