breseq  version 0.32.0  revision 6ff6de7d1b87
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 48,340 C→G 14.8% A191G (GCG→GGG)  kefC → potassium:proton antiporter
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 490,442 T→C 10.1% E124G (GAG→GGG)  priC ← primosomal replication protein N''
RA 520,561 A→G 14.3% E49G (GAG→GGG)  ybbP → putative ABC transporter permease
RA 783,885 Δ1 bp 4.3% coding (720/720 nt);coding (939/942 nt) pnuC →
zitB ←
nicotinamide riboside transporter
zinc efflux system
RA 944,918 C→T 3.7% intergenic (+22/+13) dmsC → / ← ycaC dimethyl sulfoxide reductase, anaerobic, subunit C/putative isochorismatase family hydrolase
RA 1,033,892 G→C 3.7% Q213H (CAG→CAC hyaB → hydrogenase 1, large subunit
RA 1,357,812 C→T 5.2% S459N (AGT→AAT)  puuP ← putrescine importer
RA 1,395,091 A→T 4.0% F278I (TTC→ATC)  ynaI ← mechanosensitive channel protein, very small conductance
RA 1,395,092 A→T 4.0% Y277* (TAT→TAA) ‡ ynaI ← mechanosensitive channel protein, very small conductance
RA 1,395,094 A→C 3.9% Y277D (TAT→GAT) ‡ ynaI ← mechanosensitive channel protein, very small conductance
RA 1,466,201 T→C 7.8% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,258 G→C 6.9% pseudogene (867/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,270 C→T 5.3% pseudogene (879/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,276 T→C 8.0% pseudogene (885/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,280 A→C 5.7% pseudogene (889/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,466,579 G→C 10.8% pseudogene (1188/2513 nt) ydbA → pseudogene, autotransporter homolog, interrupted by IS2 and IS30
RA 1,533,261 A→C 13.5% K70N (AAA→AAC pptA → 4‑oxalocrotonate tautomerase
RA 1,708,751 A→G 9.6% E611E (GAA→GAG rsxC → SoxR iron‑sulfur cluster reduction factor component, putative membrane‑associated NADH oxidoreductase of electron transport complex
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,665,661 G→A 5.0% E77K (GAA→AAA)  csiE → stationary phase inducible protein
JC 2,765,454 (TATGGCAC)6→5 8.8% intergenic (‑303/+50) yfjL ← / ← yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage, uncharacterized protein
RA 2,802,426 T→A 3.7% S361T (TCC→ACC)  nrdE → ribonucleoside‑diphosphate reductase 2, alpha subunit
RA 2,929,617 C→T 3.6% S15F (TCC→TTC)  sdaB → L‑serine dehydratase 2
RA 2,954,067 G→C 5.5% F645L (TTC→TTG recB ← exonuclease V (RecBCD complex), beta subunit
RA 3,248,792 T→C 4.5% L119S (TTA→TCA)  mzrA → modulator of EnvZ/OmpR regulon
RA 3,593,824 T→C 7.1% S128G (AGC→GGC)  livG ← branched‑chain amino acid ABC transporter ATPase
RA 3,688,286 A→C 11.3% V296G (GTG→GGG)  bcsC ← cellulose synthase subunit
RA 3,746,201 A→T 3.7% T37S (ACC→TCC)  yiaO → 2,3‑diketo‑L‑gulonate‑binding periplasmic protein
MC JC 3,815,858 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,945,935 T→A 5.1% noncoding (2233/2904 nt) rrlC → 23S ribosomal RNA of rrnC operon
RA 3,979,142 G→C 3.7% A181A (GCG→GCC wzyE → putative ECA polysaccharide chain elongation protein
MC JC 4,001,644 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,056,768 Δ1 bp 2.9% coding (1266/1410 nt) glnA ← glutamine synthetase
RA 4,073,312 Δ1 bp 2.8% coding (259/897 nt) yihU ← 3‑sulpholactaldehyde (SLA) reductase, NADH‑dependent, gamma‑hydroxybutyrate dehydrogenase, NADH‑dependent
RA 4,184,542 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,228,163 A→G 10.1% E145G (GAG→GGG)  yjbB → putative Na+/Pi‑cotransporter
RA 4,271,832 T→C 7.1% E680G (GAG→GGG)  uvrA ← ATPase and DNA damage recognition protein of nucleotide excision repair excinuclease UvrABC
RA 4,295,833 T→C 8.5% intergenic (+40/+604) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,059 C→T 9.8% intergenic (+266/+378) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,189 A→G 17.5% intergenic (+396/+248) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,190 A→C 22.5% intergenic (+397/+247) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4110638 4111536 899 37 [35] [35] 37 [cdh]–tpiA [cdh], tpiA

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 257908 =NA (NA)63 (0.680) 53/500 NT 100% intergenic (+9/‑768) crl/crl pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers/pseudogene, sigma factor‑binding protein, RNA polymerase holoenzyme formation stimulator,regulator, Surface structures, transcriptional regulator of cryptic csgA gene for curli surface fibers
?NC_000913 = 1293039 0 (0.000)intergenic (‑117/‑488) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
* ? NC_000913 1207790 =16 (0.170)59 (0.680) 47/468 NT 75.7% coding (290/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 1209619 = 23 (0.270)pseudogene (1/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 = 120780515 (0.160)75 (0.870) 50/468 NT 80.2% coding (305/630 nt) stfP e14 prophage, uncharacterized protein
?NC_000913 = 1209602 23 (0.270)pseudogene (18/501 nt) stfE pseudogene, e14 prophage, side tail fiber protein fragment family,Phage or Prophage Related
* ? NC_000913 1293032 =1 (0.010)111 (1.200) 75/500 NT 99.1% intergenic (‑110/‑495) hns/tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
?NC_000913 = 1979270 NA (NA)intergenic (‑55/‑483) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 12994980 (0.000)59 (0.650) 45/492 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 139508894 (1.020)4 (0.040) 4/486 NT 4.2% coding (835/1032 nt) ynaI mechanosensitive channel protein, very small conductance
?NC_000913 = 1395094 93 (1.040)coding (829/1032 nt) ynaI mechanosensitive channel protein, very small conductance
* ? NC_000913 1979486 =0 (0.000)49 (0.530) 32/500 NT 100% intergenic (‑271/‑267) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 2101749 = NA (NA)intergenic (‑5/+146) wbbL/insH1 pseudogene, lipopolysaccharide biosynthesis protein/IS5 transposase and trans‑activator
* ? NC_000913 = 19794890 (0.000)84 (0.910) 51/500 NT 100% intergenic (‑274/‑264) insA/uspC IS1 repressor TnpA/universal stress protein
?NC_000913 = 2290111 NA (NA)intergenic (‑32/+1) insH1/yejO IS5 transposase and trans‑activator/pseudogene, autotransporter outer membrane homology,putative transport, Not classified, putative ATP‑binding component of a transport system
* ? NC_000913 4295831 =NA (NA)12 (0.150) 4/422 NT NA intergenic (+38/+606) gltP/yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
?NC_000913 = 4296395 NA (NA)noncoding (562/602 nt) RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites RIP321 (repetitive extragenic palindromic) element, contains 11 REP sequences and 4 IHF sites