breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 231,861 C→T 71.8% T247M (ACG→ATG)  yafD → endo/exonuclease/phosphatase family protein
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 396,286 A→G 6.4% M1S (TTG→TCG) † ampH ← D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase
RA 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,019,613 Δ1 bp 7.5% intergenic (+100/‑5) fliK → / → fliL flagellar hook‑length control protein/flagellar biosynthesis protein
RA 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,359 Δ1 bp 8.0% pseudogene (4/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,360 Δ1 bp 9.6% pseudogene (3/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,318,095 C→G 100% L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,875,263 C→T 8.5% intergenic (‑103/‑175) dgoR ← / → yidX D‑galactonate catabolism operon transcriptional repressor/putative lipoprotein
RA 3,993,145 C→A 100% R665S (CGT→AGT)  cyaA → adenylate cyclase
RA 4,004,478 G→A 13.6% L74L (CTG→TTG)  yigI ← 4HBT thioesterase family protein
RA 4,117,047 G→T 100% S59Y (TCC→TAC)  glpK ← glycerol kinase
RA 4,131,611 A→C 66.9% T593P (ACC→CCC)  metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,183,165 G→A 100% E641K (GAA→AAA)  rpoB → RNA polymerase, beta subunit
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 2469275 2469850 576 23 [20] [22] 23 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =29 (0.430)36 (0.570) 28/422 NT 56.7% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 28 (0.450)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780530 (0.440)35 (0.560) 32/422 NT 55.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 28 (0.450)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)29 (0.440) 24/446 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3721045 =83 (1.230)164 (2.440) 92/452 NT 67.7% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 74 (1.100)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28