breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | ||||||
---|---|---|---|---|---|---|
evidence | position | mutation | freq | annotation | gene | description |
RA | 231,861 | C→T | 71.8% | T247M (ACG→ATG) | yafD → | endo/exonuclease/phosphatase family protein |
MC JC | 257,908 | Δ776 bp | 100% | [crl] | [crl] | |
RA | 396,286 | A→G | 6.4% | M1S (TTG→TCG) † | ampH ← | D‑alanyl‑D‑alanine‑ carboxypeptidase/endopeptidase; penicillin‑binding protein; weak beta‑lactamase |
RA | 1,685,527 | A→G | 100% | G354G (GGT→GGC) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
MC JC | 1,978,503 | Δ776 bp | 100% | insB1–insA | insB1, insA | |
RA | 2,019,613 | Δ1 bp | 7.5% | intergenic (+100/‑5) | fliK → / → fliL | flagellar hook‑length control protein/flagellar biosynthesis protein |
RA | 2,145,313 | C→A | 100% | S683Y (TCC→TAC) | yegE → | putative diguanylate cyclase |
RA | 2,173,359 | Δ1 bp | 8.0% | pseudogene (4/442 nt) | gatC ← | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,173,360 | Δ1 bp | 9.6% | pseudogene (3/442 nt) | gatC ← | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,173,361 | Δ2 bp | 100% | pseudogene (1‑2/442 nt) | gatC ← | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,318,095 | C→G | 100% | L594F (TTG→TTC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
RA | 3,560,455:1 | +G | 100% | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,875,263 | C→T | 8.5% | intergenic (‑103/‑175) | dgoR ← / → yidX | D‑galactonate catabolism operon transcriptional repressor/putative lipoprotein |
RA | 3,993,145 | C→A | 100% | R665S (CGT→AGT) | cyaA → | adenylate cyclase |
RA | 4,004,478 | G→A | 13.6% | L74L (CTG→TTG) | yigI ← | 4HBT thioesterase family protein |
RA | 4,117,047 | G→T | 100% | S59Y (TCC→TAC) | glpK ← | glycerol kinase |
RA | 4,131,611 | A→C | 66.9% | T593P (ACC→CCC) | metL → | Bifunctional aspartokinase/homoserine dehydrogenase 2 |
RA | 4,183,165 | G→A | 100% | E641K (GAA→AAA) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,380:1 | +CG | 100% | intergenic (+586/+56) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 2469275 | 2469850 | 576 | 23 [20] | [22] 23 | gtrS | serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 29 (0.430) | 36 (0.570) | 28/422 | NT | 56.7% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 28 (0.450) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 30 (0.440) | 35 (0.560) | 32/422 | NT | 55.6% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 28 (0.450) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 29 (0.440) | 24/446 | NT | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3721045 = | 83 (1.230) | 164 (2.440) | 92/452 | NT | 67.7% | noncoding (413/1443 nt) | IS150 | repeat region |
? | NC_000913 | = 3839774 | 74 (1.100) | coding (220/819 nt) | nlpA | cytoplasmic membrane lipoprotein‑28 |