breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 83,587 A→C 5.7% intergenic (‑58/+35) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
RA 83,590 G→T 5.3% intergenic (‑61/+32) leuA ← / ← leuL 2‑isopropylmalate synthase/leu operon leader peptide
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 915,080 G→C 6.1% intergenic (‑223/+272) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 915,081 G→C 5.8% intergenic (‑224/+271) ybjE ← / ← aqpZ putative transporter/aquaporin Z
RA 940,754 G→A 5.0% intergenic (+34/‑205) serS → / → dmsA seryl‑tRNA synthetase/dimethyl sulfoxide reductase, anaerobic, subunit A
RA 1,041,937 T→G 5.2% intergenic (+22/+93) appA → / ← etk phosphoanhydride phosphorylase/tyrosine‑protein kinase, role in O‑antigen capsule formation
RA 1,449,050 T→A 5.5% intergenic (+32/+26) feaB → / ← tynA phenylacetaldehyde dehydrogenase/tyramine oxidase, copper‑requiring
RA 1,685,527 A→G 100% G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
RA 1,703,251 A→T 5.6% intergenic (+17/+17) add → / ← ydgJ adenosine deaminase/putative oxidoreductase
RA 1,703,252 A→T 5.4% intergenic (+18/+16) add → / ← ydgJ adenosine deaminase/putative oxidoreductase
RA 1,865,682 G→C 5.6% intergenic (‑46/+44) yeaE ← / ← mipA aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent/scaffolding protein for murein synthesizing machinery
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 1,985,112 C→G 6.9% intergenic (‑43/+27) araG ← / ← araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
RA 2,145,313 C→A 100% S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,286,311 C→G 5.6% intergenic (+24/+79) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,313 T→A 5.6% intergenic (+26/+77) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,286,315 C→G 5.4% intergenic (+28/+75) proL → / ← yejO tRNA‑Pro/pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,305,085 A→G 7.7% intergenic (+692/+23) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,086 C→G 7.6% intergenic (+693/+22) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,087 C→T 7.7% intergenic (+694/+21) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,318,095 C→G 100% L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
RA 2,535,948 G→C 100% D39H (GAT→CAT)  crr → glucose‑specific enzyme IIA component of PTS
RA 2,906,608 G→C 6.4% intergenic (+25/+35) ygcG → / ← eno TPM domain protein, putative phosphatase/enolase
RA 3,171,852 C→T 5.1% intergenic (+19/+27) qseC → / ← ygiZ quorum sensing sensory histidine kinase in two‑component regulatory system with QseB/inner membrane protein
RA 3,239,609 T→G 5.7% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,439,588 G→C 7.6% intergenic (‑79/+28) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17
RA 3,439,589 G→C 7.2% intergenic (‑80/+27) yhdN ← / ← rplQ DUF1992 family protein/50S ribosomal subunit protein L17
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,739,698 T→C 5.5% intergenic (+171/‑7) malS → / → avtA alpha‑amylase/valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
RA 3,739,717 C→T 7.0% L5F (CTT→TTT)  avtA → valine‑pyruvate aminotransferase; transaminase C; alanine‑valine transaminase
RA 3,891,502 C→G 6.4% intergenic (+19/‑113) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,504 T→A 6.4% intergenic (+21/‑111) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 3,891,506 C→G 6.0% intergenic (+23/‑109) tnaB → / → mdtL tryptophan transporter of low affinity/multidrug efflux system protein
RA 4,115,901 G→A 100% A441V (GCA→GTA)  glpK ← glycerol kinase
RA 4,296,060 C→T 25.8% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423731–3424234 3424562–3424238 5–832 154 [151] [146] 153 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)4 (0.020) 4/254 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 228359 =NA (NA)3 (0.010) 3/256 NT NA noncoding (2601/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
?NC_000913 228397 = NA (NA)noncoding (2639/2904 nt) rrlH 23S ribosomal RNA of rrnH operon
* ? NC_000913 274620 =NA (NA)6 (0.020) 4/250 NT NA noncoding (530/1195 nt) IS5 repeat region
?NC_000913 274663 = NA (NA)noncoding (487/1195 nt) IS5 repeat region
* ? NC_000913 = 381823NA (NA)3 (0.010) 3/258 NT NA noncoding (564/1331 nt) IS2 repeat region
?NC_000913 = 381866 NA (NA)noncoding (607/1331 nt) IS2 repeat region
* ? NC_000913 430968 =NA (NA)7 (0.030) 4/254 NT NA noncoding (1/98 nt) REP37 (repetitive extragenic palindromic) element; contains 2 REP sequences REP37 (repetitive extragenic palindromic) element; contains 2 REP sequences
?NC_000913 430993 = NA (NA)noncoding (26/98 nt) REP37 (repetitive extragenic palindromic) element; contains 2 REP sequences REP37 (repetitive extragenic palindromic) element; contains 2 REP sequences
* ? NC_000913 = 984461NA (NA)7 (0.030)
+TAAC
7/260 NT NA intergenic (+164/+58) ycbL/aspC putative metal‑binding enzyme/aspartate aminotransferase, PLP‑dependent
?NC_000913 3774201 = NA (NA)intergenic (+7/‑223) mtlA/mtlD mannitol‑specific PTS enzyme: IIA, IIB and IIC components/mannitol‑1‑phosphate dehydrogenase, NAD‑dependent
* ? NC_000913 1207790 =95 (0.340)169 (0.680) 97/236 NT 65.6% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 94 (0.380)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780598 (0.350)155 (0.630) 101/236 NT 63.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 94 (0.380)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)309 (1.140) 148/260 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2728214 =NA (NA)9 (0.030) 5/260 NT NA noncoding (971/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
?NC_000913 2728240 = NA (NA)noncoding (945/2904 nt) rrlG 23S ribosomal RNA of rrnG operon
* ? NC_000913 2729475 =134 (0.480)6 (0.020) 3/248 NT 5.5% intergenic (‑31/+141) gltW/rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
?NC_000913 = 4168363 84 (0.320)intergenic (+163/‑9) rrsB/gltT 16S ribosomal RNA of rrnB operon/tRNA‑Glu
* ? NC_000913 3721045 =326 (1.160)773 (2.780) 210/266 NT 71.9% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 280 (1.010)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28
* ? NC_000913 4542682 =212 (0.760)13 (0.050) 13/250 NT 5.2% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 279 (1.070)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)