breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 1,685,527 | A→G | G354G (GGT→GGC) | fumC ← | fumarate hydratase (fumarase C),aerobic Class II |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
RA | 2,145,313 | C→A | S683Y (TCC→TAC) | yegE → | putative diguanylate cyclase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,318,095 | C→G | L594F (TTG→TTC) | rcsC ← | hybrid sensory kinase in two‑component regulatory system with RcsB and YojN |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
JC JC | 3,992,705 | IS5 (+) +4 bp | coding (1553‑1556/2547 nt) | cyaA → | adenylate cyclase |
RA | 4,117,005 | T→G | D73A (GAT→GCT) | glpK ← | glycerol kinase |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1299331 | 1300773 | 1443 | 54 [51] | [53] 55 | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
* | * | ÷ | NC_000913 | 2469105 | 2469850 | 746 | 54 [53] | [53] 54 | [gtrB]–[gtrS] | [gtrB],[gtrS] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 82 (0.620) | 46 (0.370) | 39/482 | 2.4 | 37.9% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 74 (0.600) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 82 (0.620) | 64 (0.520) | 44/482 | 1.9 | 45.9% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 74 (0.600) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | 3721045 = | 165 (1.250) | 464 (3.520) | 173/512 | 0.0 | 76.3% | noncoding (413/1443 nt) | IS150 | repeat region |
? | NC_000913 | = 3839774 | 124 (0.940) | coding (220/819 nt) | nlpA | cytoplasmic membrane lipoprotein‑28 |