breseq  version 0.26.1  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 697,151 A→G intergenic (‑18/+362) metT ← / ← asnB tRNA‑Met/asparagine synthetase B
JC JC 757,368 IS5 (–) +3 bp coding (1462‑1464/1767 nt) sdhA → succinate dehydrogenase, flavoprotein subunit
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,245 T→C L375L (TTA→CTA)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,247 A→G L375L (TTA→TTG icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,292 C→T T390T (ACC→ACT icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,304 G→A E394E (GAG→GAA icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,316 T→A D398E (GAT→GAA icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,196,325 A→G K401K (AAA→AAG icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
RA 1,442,789 C→A V19L (GTG→TTG)  ldhA ← fermentative D‑lactate dehydrogenase, NAD‑dependent
MC JC 1,674,993 1365 bp→81 bp coding (4‑1368/1389 nt) pntB ← pyridine nucleotide transhydrogenase, beta subunit
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,213,608 C→A G346V (GGG→GTG)  yehU ← inner membrane putative sensory kinase in two‑component system with YehT
RA 3,053,437 +G coding (82/1179 nt) ubiH ← 2‑octaprenyl‑6‑methoxyphenol hydroxylase, FAD/NAD(P)‑binding
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
MC JC 3,915,584 6827 bp→81 bp [atpC][atpI] [atpC], atpD, atpG, atpA, atpH, atpF, atpE, atpB, [atpI]
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1166027 1167457 1431 9 [7] [8] 9 ndh ndh
* * ÷ NC_000913 1196334 1211448 15115 9 [8] [8] 9 [icd]–[icdC] 25 genes
[icd], ymfD, ymfE, lit, intE, xisE, ymfI, ymfJ, cohE, croE, ymfL, ymfM, oweE, aaaE, ymfR, beeE, jayE, ymfQ, stfP, tfaP, tfaE, stfE, pinE, mcrA, [icdC]
* * ÷ NC_000913 3423820–3424234 3424498–3424238 5–679 9 [8] [8] 9 [rrlD] [rrlD]

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)34 (0.920) 33/410 0.2 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein