Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,723,459 T→C 100% T91T (ACT→ACC)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,4590TC100.0% 203.3 / ‑6.6 56T91T (ACT→ACC) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (17/38);  minor base A (1/0);  total (18/38)
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

CTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTT  >  CP006881/3723344‑3723592
                                                                                                                   |                                                                                                                                     
ttATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCGATTATGATGATTGCTTACGGAGGGGGTGATTTTTTTTACGGCAACACCTTTTTTTTGCCCTATACCGCGCGCCCgtaaaaaa                                                                                                                 >  4:9765/2‑138 (MQ=255)
  aTGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGGAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAAtca                                                                                                              >  5:107020/1‑137 (MQ=255)
  aTGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCAGAAGATTGCTTAAGGACGCCGAGAATTAATTACCCGCCACCCCATTTATTTAACCTAAACCCCGCACCGgaaaaaaaaa                                                                                                               >  2:367610/1‑136 (MQ=255)
      ctgATTCTCCTTTTGCCTTTCCGGCATCCGCTGTCACTTTCGGGGCCGCTATTCTTATGATTGCTTACGGAGGAGTAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTg                                                                                                          <  4:39642/137‑1 (MQ=255)
       tgATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTgg                                                                                                         <  6:371363/138‑1 (MQ=255)
         aTTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACCCCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCg                                                                                                       >  2:4298/1‑139 (MQ=255)
           cagccGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                     <  5:116036/136‑3 (MQ=255)
            ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                     <  7:308829/138‑3 (MQ=255)
            ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                     <  7:258833/138‑3 (MQ=255)
            ctcCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                     <  8:294711/138‑3 (MQ=255)
            ctcCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcg                                                                                                     <  3:9765/138‑3 (MQ=255)
            ctcCGGTTGCCTTTCCGGCAGCCGCTGTCACGTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCTACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  2:87380/139‑1 (MQ=255)
              ccGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                                                  <  8:154052/139‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  7:165538/136‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  3:177477/136‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  6:352454/136‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  7:57841/136‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  5:283036/136‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGacac                                                                                                 <  1:25235/139‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                                                  <  2:30027/138‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGaca                                                                                                  <  3:121436/138‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  7:284455/136‑1 (MQ=255)
               cGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  1:266283/136‑1 (MQ=255)
               cGTTTGCCTCTCCGGCAGCCGCTGTCACTATCGGCGCCGCTTTGCTGATGATTGCTTACGGCGGCGTAGATTTATTTACCGTCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGctcga                                                                                                    <  7:38907/136‑5 (MQ=255)
                gTTTGCCTTTCCGGCAGGCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACTcc                                                                                                <  7:332004/139‑1 (MQ=255)
                gTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACAcc                                                                                                <  4:84655/139‑1 (MQ=255)
                 tttGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCTGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACAccc                                                                                               <  5:308849/139‑1 (MQ=255)
                    gcctttCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTAGGATGATTGCTTACGGAGGCGGCGATTTATTTACCGGCCACCCCGTTTATTTTAACTATAACGCGCGCCGGAAAAAAATCAGCTTGCGCGCCCCCCta                                                                                             >  2:134019/4‑137 (MQ=255)
                         tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTTTTCTGATGATTGCGTTCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTAACCTTTACCGCGCGCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtg                                                                                       >  7:154052/1‑139 (MQ=255)
                         tCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTATGATGATTGCTTAAGGAGGCGGAGATTTATTTTACGGCAACACCTTTTATTTGACATATAACGCGCTCCGGAAAAAAATAATCTTGCGCGACACCCGATACCtg                                                                                       >  6:116036/1‑139 (MQ=255)
                           cGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgtg                                                                                     <  2:229733/139‑1 (MQ=255)
                                                 ggCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    <  4:303497/102‑1 (MQ=255)
                                                 ggCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCgcga                                                                                                    >  3:303497/1‑102 (MQ=255)
                                                      cttttCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGTCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                          <  4:128276/136‑1 (MQ=255)
                                                      cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                          <  2:293638/139‑1 (MQ=255)
                                                      cTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAAt                                                          <  7:168623/139‑1 (MQ=255)
                                                         ttCTGATGATTGCTTACGGAGTCGGAGATTTATTTACCTGCAACACCTTTTATTTAACCTATACCGCGCTCCTGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCgg                                                       <  7:275958/139‑1 (MQ=255)
                                                             gatgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGgcgc                                                    >  5:143533/3‑138 (MQ=255)
                                                              atcaatGCTTACGGAGGCGGAGATTTATTTAAAGTAAAAAACTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGAAACCCTATACTTGTGTATGTCAAGCTATGCCGGCAATTTAATCGGCGACa                                                  >  6:98823/6‑139 (MQ=255)
                                                                 aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTc                                               >  7:177249/1‑139 (MQ=255)
                                                                 aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTc                                               >  8:341613/1‑139 (MQ=255)
                                                                   tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtg                                             >  5:29728/1‑138 (MQ=255)
                                                                   tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtg                                             >  6:13872/1‑138 (MQ=255)
                                                                   tGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCtg                                             >  6:111907/1‑138 (MQ=255)
                                                                     cTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgt                                                                                      <  3:302229/96‑1 (MQ=255)
                                                                     cTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTtgt                                                                                      >  4:302229/1‑96 (MQ=255)
                                                                        aCGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTg                                         <  1:367610/138‑1 (MQ=255)
                                                                             ggCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                              >  8:358075/1‑128 (MQ=255)
                                                                             ggCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCt                                              <  7:358075/128‑1 (MQ=255)
                                                                                    atttatttACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAg                            <  7:341613/139‑1 (MQ=255)
                                                                                    atttatttACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAg                            <  4:342180/139‑1 (MQ=255)
                                                                                                    cacctattATTTCACCTATACCGCGCTCCGGAAAAAACTCCGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACttttt            <  6:29728/133‑1 (MQ=255)
                                                                                                       cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag         <  8:177249/138‑1 (MQ=255)
                                                                                                       cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag         >  1:238919/2‑139 (MQ=255)
                                                                                                          ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgagga       <  1:134019/138‑1 (MQ=255)
                                                                                                               tCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt  <  1:353362/138‑1 (MQ=255)
                                                                                                                   |                                                                                                                                     
CTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTT  >  CP006881/3723344‑3723592

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑