Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	4,010,978	G→T	6.2%	C618F (TGC→TTC)	U712_20715 →	Uncharacterized protein yyaL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	4,010,978	0	G	T	6.2%	198.2 / 3.1	65	C618F (TGC→TTC)	U712_20715	Uncharacterized protein yyaL
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base T (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.20e-01

TCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAGCAGCAGGGA  >  CP006881/4010847‑4011116                                                                                                                                    |                                                                                                                                           tCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGCCTCCATTCTGGTGGCAGAGCAGCCCGAGCACTGCAAAGa                                                                                                                                      >  3:68529/1‑138 (MQ=255) tCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGa                                                                                                                                      >  7:247309/1‑138 (MQ=255) tCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGa                                                                                                                                      >  5:223219/1‑138 (MQ=255) tCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGa                                                                                                                                      >  2:352829/1‑138 (MQ=255) tCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGa                                                                                                                                      >  8:338040/1‑138 (MQ=255)       caaaaaaaaaTATATCTTTTTTTTTGGCAGCCCTGACGAACCCCAAATAAAAAAAATTATGGCGGAGATACAAAAAGCCTTAACGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTgcg                                                                                                                               <  4:337635/135‑1 (MQ=255)        ccTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTgcgc                                                                                                                              >  4:267713/1‑139 (MQ=255)        ccTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTgcgc                                                                                                                              >  7:51753/1‑139 (MQ=255)            aaaaaTACATCGGGATTTTTGGCAGCGCAGCCGAACCAGCCAGAAAAAAAATTAACGCGGAGCTTAAAAAAGCATTTAACCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCAtt                                                                                                                          <  7:351183/139‑1 (MQ=255)              aaaCAAATGGTTATTTTTGGCACCGCAGACGATCCACACACAAAAAAAATTATAGGGGAGCTTCAAAAAGCATTTAACCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTg                                                                                                                        <  8:51753/139‑1 (MQ=255)                   actCGTTATTTTGGGCAGCCCAGACGATCCACCCATAAAACAAATTATCGCGGAGCTTAAAAAACCCTTTAAGCCCCACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTgcagca                                                                                                                   <  3:281647/137‑1 (MQ=255)                    aTCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCCGCCCGAGCAATTCAAAGACATTGCGCCATTTgcag                                                                                                                     >  5:342435/1‑136 (MQ=255)                    aTCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATTCAAAGACATTGCGCCATTTgcag                                                                                                                     >  5:347799/1‑136 (MQ=255)                        ctaTTTTTGGCCCCCCCGACCATCCAGCCACAAAAAAATTAAAAGCGGCGGTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTa                                                                                                              <  8:338637/137‑1 (MQ=255)                         gATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTAc                                                                                                             >  6:280781/1‑139 (MQ=255)                                  cAGCGCCGACGATCCAGCCAGAAAAAAAATTATAGCGGAGCTTCAAAAAGCCTTAAAGCCCCACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATa                                                                                                    <  7:40178/139‑1 (MQ=255)                                             atccccccatAAAAAAATTTATAGCGGCGCTTCAAAAAGCCTTTACGCCCCACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATTCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAaac                                                                                         <  6:342435/133‑1 (MQ=255)                                             agccccccatAAAACAAATAATCGGGGAGCTTAAAAAAGCCTTACCGCCCCACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAaac                                                                                         <  1:278665/133‑1 (MQ=255)                                             acccccccatAAAACAATTTATCGGGGAGATTCAAAACGCTTTTACGCCAAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAaac                                                                                         <  1:23601/133‑1 (MQ=255)                                              accagccatAAAACAAATTATAGCGGAGCTTCAAAACGCATTAACCCCCCACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATTCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAaac                                                                                         <  6:347799/137‑1 (MQ=255)                                               ccagccagAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAaacaa                                                                                       >  3:299379/1‑139 (MQ=255)                                               ccagccagAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAaacaa                                                                                       >  3:45415/1‑139 (MQ=255)                                                  gccagAAAACAAATTATAGAGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCTATGCAAAGACATTGCGCAATTTGCAGCAGACTACCGCATCATAGAAGGCAAAACAACCg                                                                                    >  3:5827/1‑139 (MQ=255)                                                                          cTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCt                                                            >  1:15733/1‑139 (MQ=255)                                                                                          cgCCCAACGACTCCATTCTGGTGGCAGCGCAGCCCGAGCAATGCAAAGACATTGCGCCAATTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGACAACTTCGCCTGCCAGCAACCGacaac                                            <  6:261258/138‑1 (MQ=255)                                                                                              cAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCTCCTGCCAGCAACCGACAACaaa                                         >  5:189135/1‑138 (MQ=255)                                                                                              cAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACaaa                                         >  4:176326/1‑138 (MQ=255)                                                                                               aaCGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAata                                       >  6:161596/1‑139 (MQ=255)                                                                                               aaCGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAata                                       >  5:338584/1‑139 (MQ=255)                                                                                               aaCGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATATGTGAAAACTACGCCTGCCAGCAACCGACAACAAata                                       >  6:53023/1‑139 (MQ=255)                                                                                                aCGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAatat                                      >  4:276059/1‑139 (MQ=255)                                                                                                       cATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACACCCGTTTATATTTGTGAAAACTTCGCCTGCCAGCACCCGACAACAAATCTTgaag                                 >  3:215803/1‑137 (MQ=255)                                                                                                       cATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTgaag                                 <  1:27311/137‑1 (MQ=255)                                                                                                       cATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTgaag                                 <  1:27317/137‑1 (MQ=255)                                                                                                        aTTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTCCCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGaagaag                              <  1:358315/139‑1 (MQ=255)                                                                                                        aTTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGaagaag                              <  8:56351/139‑1 (MQ=255)                                                                                                        aTTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGaagaag                              <  4:299379/139‑1 (MQ=255)                                                                                                        aTTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGaagaag                              <  4:45415/139‑1 (MQ=255)                                                                                                         ttCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGc                             >  1:253490/1‑139 (MQ=255)                                                                                                           cTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCg                            <  5:161596/138‑1 (MQ=255)                                                                                                           cTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCg                            <  4:36648/138‑1 (MQ=255)                                                                                                            tggtggCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGAt                          >  7:267697/1‑139 (MQ=255)                                                                                                              gtggCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATc                         >  8:235956/1‑138 (MQ=255)                                                                                                               tggCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCca                       <  3:163129/139‑1 (MQ=255)                                                                                                                 gCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCTCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCcaca                     <  7:338040/139‑1 (MQ=255)                                                                                                                 gCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCcaca                     <  3:267713/139‑1 (MQ=255)                                                                                                                 gCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCcaca                     <  3:304241/139‑1 (MQ=255)                                                                                                                   agagCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCcacaca                   >  4:362732/1‑139 (MQ=255)                                                                                                                        agcCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATtaat              <  8:247309/139‑1 (MQ=255)                                                                                                                          cccGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATtaata             >  8:227435/1‑138 (MQ=255)                                                                                                                          cccGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATtaat              >  6:241475/1‑137 (MQ=255)                                                                                                                           ccGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGacaac                                            >  4:283683/1‑106 (MQ=255)                                                                                                                           ccGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGacaac                                            <  3:283683/106‑1 (MQ=255)                                                                                                                              aGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAagcag        <  5:241475/139‑1 (MQ=255)                                                                                                                                cAATGCAAAGACATTGCGCCATTTGCAGCCGACTACCGACTCATAGACGGCAAAACAACAGTTTATATTTGTGAAAACTTCGCCTGCTAGCAATCTACAACAAATATTGAATAAGCGATCCACACATTAATAAgcagca      <  4:331664/139‑1 (MQ=255)                                                                                                                                cAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAgcagca      <  7:117019/139‑1 (MQ=255)                                                                                                                                cAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAgcagca      <  7:235956/139‑1 (MQ=255)                                                                                                                                cAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACAGACAACAAATATTGAATAAGCGATCAACACATTAATAAgcagca      <  4:357200/139‑1 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCg                                                 <  1:213348/93‑1 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCg                                                 >  2:213348/1‑93 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGacaac                                            >  3:353091/1‑98 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGacaac                                            <  4:353091/98‑1 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAGCAGCAgg    >  3:3240/1‑138 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAGCAGCAgg    >  4:257022/1‑138 (MQ=255)                                                                                                                                   tGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTAGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAGCAGCAgg    >  8:35890/1‑138 (MQ=255)                                                                                                                                    gCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGCTCCACACATTAATAAGCAGCAGGGa  >  7:87411/1‑139 (MQ=255)                                                                                                                                    gCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAgcagca      >  2:27572/1‑135 (MQ=255)                                                                                                                                    gCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAgcagca      <  1:27572/135‑1 (MQ=255)                                                                                                                                    |                                                                                                                                           TCTGATGCCTAAAAAAGAAATCGTGATTTTTGGCAGCGCAGACGATCCAGCCAGAAAACAAATTATAGCGGAGCTTCAAAAAGCCTTTAAGCCCAACGACTCCATTCTGGTGGCAGAGCAGCCCGAGCAATGCAAAGACATTGCGCCATTTGCAGCAGACTACCGCATCATAGACGGCAAAACAACCGTTTATATTTGTGAAAACTTCGCCTGCCAGCAACCGACAACAAATATTGAAGAAGCGATCCACACATTAATAAGCAGCAGGGA  >  CP006881/4010847‑4011116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑