Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,119,432 A→T 8.4% G870G (GGA→GGT)  U712_05510 → Cell wall‑associated protease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,119,4320AT 8.4% 151.0 / 3.0 56G870G (GGA→GGT) U712_05510Cell wall‑associated protease
Reads supporting (aligned to +/- strand):  ref base A (23/28);  new base T (2/3);  total (25/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGACTGCTGT  >  CP006881/1119295‑1119564
                                                                                                                                         |                                                                                                                                    
aaGGATATGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  8:7607/139‑1 (MQ=255)
aaGGATACGGCAGTAAAAGTGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  5:177290/139‑1 (MQ=255)
aaGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  8:263448/139‑1 (MQ=255)
aaGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  8:2131/139‑1 (MQ=255)
aaGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  7:60105/139‑1 (MQ=255)
 aGGTTACGTCATTAAAAGTAAAGGCAAACAGCAAAGCTATTATAACAGTTAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  8:346380/138‑1 (MQ=255)
 aGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  6:364739/138‑1 (MQ=255)
  ggATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  5:283540/137‑1 (MQ=255)
  ggATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     >  6:283540/1‑137 (MQ=255)
     tACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTg                                                                                                                                <  7:290724/139‑1 (MQ=255)
        ggCAGTAAAAGGGAAGGCAAACAGGAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGAAGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTg                                                                                                                             <  1:7074/139‑1 (MQ=255)
        ggCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTg                                                                                                                             <  4:220800/139‑1 (MQ=255)
        ggCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTg                                                                                                                             <  4:69936/139‑1 (MQ=255)
        cgCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCTTTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTATCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTg                                                                                                                             <  5:265362/138‑1 (MQ=255)
             tAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGt                                                                                                                        >  4:30769/1‑139 (MQ=255)
             tAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGt                                                                                                                        >  4:296285/1‑139 (MQ=255)
               aaaGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCa                                                                                                                      <  6:261753/139‑1 (MQ=255)
               aaaGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCa                                                                                                                      <  6:255091/139‑1 (MQ=255)
               aaaGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCa                                                                                                                      <  2:52719/139‑1 (MQ=255)
               aaaGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCa                                                                                                                      <  4:306688/139‑1 (MQ=255)
                  gggAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACgg                                                                                                                   >  8:318128/1‑139 (MQ=255)
                    gAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     >  7:126728/1‑119 (MQ=255)
                    gAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  8:126728/119‑1 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     <  6:156238/116‑1 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGaa                                                                                                                                     >  5:156238/1‑116 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGTACGGTGCTGTACGTCACGGCtgtg                                                                                                              >  1:115212/1‑139 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGTACGGTGCTGTACGTCACGGCtgtg                                                                                                              >  1:109734/1‑139 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCtgtg                                                                                                              >  5:109708/1‑139 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCtgtg                                                                                                              >  4:98734/1‑139 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCtgtg                                                                                                              >  6:364841/1‑139 (MQ=255)
                       ggCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGCTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCtgtg                                                                                                              >  5:236660/1‑139 (MQ=255)
                                  aaGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATa                                                                                                    >  8:197830/1‑138 (MQ=255)
                                   aGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATaa                                                                                                   >  5:176613/1‑138 (MQ=255)
                                        tgatCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAACGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAaaag                                                                                              >  6:82153/1‑138 (MQ=255)
                                        tgatCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCa                                                                                                                      >  4:316455/1‑114 (MQ=255)
                                        tgatCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCa                                                                                                                      <  3:316455/114‑1 (MQ=255)
                                          atCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCATAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAaaaga                                                                                             >  1:229291/1‑137 (MQ=255)
                                          atCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAaaaga                                                                                             >  1:282450/1‑137 (MQ=255)
                                               agTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAg                                                                                      >  1:22182/1‑139 (MQ=255)
                                               agTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAg                                                                                      >  2:272458/1‑139 (MQ=255)
                                                 tGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACGGATAAAAAAGAAAGCAAGGa                                                                                    <  6:324501/139‑1 (MQ=255)
                                                           aTCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAg                                                                            >  2:172197/1‑137 (MQ=255)
                                                            tCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAgttg                                                                         <  3:98734/139‑1 (MQ=255)
                                                                                  ccAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAg                                                   >  2:353403/1‑139 (MQ=255)
                                                                                  ccAAAGCTGACGAAAAAGGAACGTTTTAGTTGAAAATCAAAAAACAAAAACCCGGAACGGTGCTGTACGTCACGGCTGTTGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAg                                                   <  4:243978/139‑1 (MQ=255)
                                                                                      aGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGt                                               >  7:87278/1‑139 (MQ=255)
                                                                                          gACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACg                                                                                                                                   >  4:202748/1‑51 (MQ=255)
                                                                                          gACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACg                                                                                                                                   <  3:202748/51‑1 (MQ=255)
                                                                                           aCGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCt                                            <  3:147037/137‑1 (MQ=255)
                                                                                           aCGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTc                                           >  2:280158/1‑138 (MQ=255)
                                                                                            cGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGa                                         >  4:328934/1‑139 (MQ=255)
                                                                                                     aaCGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCt                                >  1:127535/1‑139 (MQ=255)
                                                                                                     aaCGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCt                                >  2:215757/1‑139 (MQ=255)
                                                                                                              ggTGAAAATCAAAAAACAAAAAGCCGGATCGGTGCTGTACGTCTCGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTAttt                       <  2:229291/139‑1 (MQ=255)
                                                                                                                   aaaTCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTgcgc                   <  7:318128/138‑1 (MQ=255)
                                                                                                                     aTCAAAAATCTAAAAGTCGGTACGGTTCTGTACGTCACGGCTCTGGATACTGATAAAAAAGAAAGCAATGAAGCAAATTTTGTTGTTGAAAAGTAACCAAAAATCGGGGCTCGATGCACCGCTTTTTTATTTGCGcccg                <  8:174192/139‑2 (MQ=255)
                                                                                                                     aTCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGcccc                <  7:47479/139‑1 (MQ=255)
                                                                                                                            aaCAAAAAGCCGGTACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGa         <  2:115212/139‑1 (MQ=255)
                                                                                                                            aaCAAAAAGCCGGTACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGa         <  2:109734/139‑1 (MQ=255)
                                                                                                                            aaCAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGa         <  3:145137/139‑1 (MQ=255)
                                                                                                                            aaCAAAAAGCCGGAACGGAGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGa         <  6:271530/139‑1 (MQ=255)
                                                                                                                                aaaaTCCGGAAAGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAATGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGActgc     <  4:341007/139‑1 (MQ=255)
                                                                                                                                 aaaGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGActgct    <  7:197830/139‑1 (MQ=255)
                                                                                                                                 aaaGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGActgct    <  1:353403/139‑1 (MQ=255)
                                                                                                                                 aaaGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGActgct    <  5:154891/139‑1 (MQ=255)
                                                                                                                                 aaaGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGActgct    <  3:296285/139‑1 (MQ=255)
                                                                                                                                 aaaGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGActgct    <  3:30769/139‑1 (MQ=255)
                                                                                                                                 aaaGCAGGAAAGGTGATGTACGTCACGTCTGTGGATACAGATAAAAAAGAAAGCAATGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGGGCTCGATGCACCGATTTTTTATTTGCGCCCCCGTTGGActgct    <  2:282450/139‑1 (MQ=255)
                                                                                                                                   aGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGACTGCtgt  <  2:127535/139‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                    
AAGGATACGGCAGTAAAAGGGAAGGCAAACAGCAAAGCGATGATCAGAGTGAAAAACAAATCAAAGAAAGTCATTGCTTCTGCCAAAGCTGACGCAAAAGGAACGTTTTCGGTGAAAATCAAAAAACAAAAAGCCGGAACGGTGCTGTACGTCACGGCTGTGGATACAGATAAAAAAGAAAGCAAGGAAGCAAAAGTTGTTGTTGAAAAGTAACCAAAAAGCGGTGCTCGATGCACCGCTTTTTTATTTGCGCCCCCGTTGGACTGCTGT  >  CP006881/1119295‑1119564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑