BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,743	A→T	5.3%	intergenic (+28/‑128)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,743	0	A	T	5.3%	12983.5 / 97.2	3976	intergenic (+28/‑128)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (265/3431); new base T (248/8); total (536/3440)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4077 total aligned reads displayed.

CAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3605‑3877

 aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                         <  8:306575/138‑1 (MQ=255)

 aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                         <  7:225305/138‑1 (MQ=255)

 aGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGa                                                                                                                                         <  1:100245/138‑1 (MQ=255)

       atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑a                                                                                                                                  <  1:63661/138‑1 (MQ=255)

       atcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑a                                                                                                                                  <  2:68489/138‑1 (MQ=255)

        tcaAGCAATTAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  1:354414/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  8:177433/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  8:147598/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  7:335951/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  5:67802/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  4:363266/139‑1 (MQ=255)

        tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTAACATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑aca                                                                                                                                <  8:12023/139‑1 (MQ=255)

          aatcAATGAAACACGCCAAATTAAACAATTTAAGTACCATTAATTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  4:160184/136‑1 (MQ=255)

          aaGCAATTAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  1:124402/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  5:169357/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  6:105770/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  1:202958/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  1:90886/139‑1 (MQ=255)

          aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGCATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  5:188254/139‑1 (MQ=255)

          aaGCAATGAAACAAGACAATGTATAGAATTTTAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACg                                                                                                                              <  3:296999/139‑1 (MQ=255)

           aGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGt                                                                                                                             <  7:45684/139‑1 (MQ=255)

           aGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGt                                                                                                                             <  5:120353/139‑1 (MQ=255)

               aggAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTAAGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACt                                                                                                                         <  2:153745/137‑1 (MQ=255)

               aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACt                                                                                                                         <  3:372000/139‑1 (MQ=255)

                 gAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGTTAATTT‑AAAAGTTAATa                                                                                                                        >  4:95353/1‑138 (MQ=255)

                  aaaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTaaa                                                                                                                      <  8:280585/139‑1 (MQ=255)

                   aaCACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTaaa                                                                                                                      <  4:299716/138‑1 (MQ=255)

                       cGCCAAAGTAAACAATTTTAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGaa                                                                                                                 <  2:320721/139‑1 (MQ=255)

                       cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGaa                                                                                                                 <  6:365698/139‑1 (MQ=255)

                       cGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACAGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGaa                                                                                                                 <  1:253400/139‑1 (MQ=255)

                        gCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGTAACTT‑ACACGGTACTAAAGGGTAt                                                                                                                >  6:183104/1‑139 (MQ=255)

                               tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  2:37510/139‑1 (MQ=255)

                                aaaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATa                                                                                                         <  3:80784/138‑1 (MQ=255)

                                 aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  8:50203/139‑1 (MQ=255)

                                 aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATaaa                                                                                                       <  1:170265/139‑1 (MQ=255)

                                  aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTTGAAATTT‑TCACGTTAATAAAGGGAATGGTGATTAat                                                                                                      >  6:156559/1‑139 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGTGAAGTT‑TAACGTTTCTATATGGAATGTGGATAAattatt                                                                                                  >  6:261104/1‑139 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:286667/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:263255/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:181463/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  4:328350/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  5:142937/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  7:294062/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  3:249991/139‑1 (MQ=255)

                                      tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGACGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                  <  3:223619/139‑1 (MQ=255)

                                         aaGTACCATTTCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                <  4:10069/138‑1 (MQ=255)

                                          aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGa                                                                                               <  7:108420/138‑1 (MQ=255)

                                           gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGTAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  7:270052/139‑1 (MQ=255)

                                           gTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGata                                                                                             <  3:55672/139‑1 (MQ=255)

                                                   aCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTGTTTTATTTTTTGATTTTT‑A‑AAGTTAAGAAAATGTATTGAGATAAATAATTATTTATaatact                                                                                      >  3:17782/1‑138 (MQ=255)

                                                   aCTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATactact                                                                                      <  6:342283/138‑1 (MQ=255)

                                                       aTGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  6:84877/137‑1 (MQ=255)

                                                       aTGATCAAGTATTGTCTATTTTTAATAGTTATATATTATTTAACGGGAGGAAATAATTCTATGAGTAGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                   <  7:69024/137‑1 (MQ=255)

                                                        tGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAg                                                                                 <  6:127597/138‑1 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  3:343714/139‑1 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  7:203863/139‑1 (MQ=255)

                                                             aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                           <  8:243349/139‑1 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                          <  5:3966/139‑1 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTATATTA‑TAACCATAATAAAGGGAAAGGAGATAAATTATTATATATAATAATTACAGcataca                                                                           >  5:210386/1‑133 (MQ=255)

                                                              aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAGTTAAGAGGTTA‑TAAATGGGATTGTGTTAAATTATTTTATTTAATAATGAGAGccggtgg                                                                          >  4:261362/1‑133 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTACCGGGAGGAAATAATTCTATGAGTCGG‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  8:32752/139‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTTATATTT‑AAAAGTTAATTAAGCGTATGGAGATAAATTATTATATATACTAATTACATCttac                                                                            >  7:356106/1‑134 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                         <  6:219657/139‑1 (MQ=255)

                                                               gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTAAATTT‑TAACCTTTATAAAAGGTATGTTGTTAAATTTTTTTATTTAATACTTAAAGCtgaa                                                                            >  6:290668/1‑133 (MQ=255)

                                                                 aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                        <  1:296587/138‑1 (MQ=255)

                                                                    gTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGTAAGTT‑TCACGTTACTAAAGGGCATGGTGATAAATTATTATATATACTAAAGAAACCGTCCCAGAAGc                                                                     >  7:315373/1‑138 (MQ=255)

                                                                       tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGTAATTT‑AAACGTTTCTAAAGTGTATTGATATTAATTATTAGTTATTCTAAGTACAGCTTCGAAGAAGGTaaa                                                                 >  7:126890/1‑139 (MQ=255)

                                                                       tATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTTAAAATT‑AAACCTTTATTAATGGAATGGTGATAAATTTTTTTATATACTAATTACAACTTCCaagaatcgaa                                                                  >  2:199352/1‑133 (MQ=255)

                                                                         tttttATTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                <  1:148757/138‑1 (MQ=255)

                                                                          ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAAATT‑AAACGTTAATAAATGGAATTGAGATAAATTATTATATTTACTAATTAAACCATCCAATAAGATAAagag                                                              >  2:117104/1‑139 (MQ=255)

                                                                           tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAAGGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                              <  2:260998/138‑1 (MQ=255)

                                                                                tAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCt                                                        <  4:183963/139‑1 (MQ=255)

                                                                                  gTTATCTATTATTTCACGGGAGCAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACTTTACTAAAGCGAATGGAGATAAATTATTAGATATACTACTGTCAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                      <  3:200284/139‑1 (MQ=255)

                                                                                     aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTTAAATTT‑AAACATTTCTTAATTGTATTGAGATTAATTATTTTATATAATAATGACAACTTACAATAAACTAAAAATGTCCATatcac                                                   >  8:209821/1‑135 (MQ=255)

                                                                                            attTAACGGGAGGAAATAATGCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAAATATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                             <  6:320133/138‑1 (MQ=255)

                                                                                             ataaaCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                            <  1:318373/135‑1 (MQ=255)

                                                                                               tAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGa                                          <  8:355851/138‑1 (MQ=255)

                                                                                                  cGGGAGGAAATAATTCTATGAGTCGCGGTTTTTAAATTT‑GTAAGTT‑TAACGGTTCTAAAGGGTATTGGGATTAATTATTAGATATTCTACTGACAGCGTGCCAGAAGCTAAAGTGGGCGCAAGCGCCGACGGGGAGttt                                      >  8:72808/1‑139 (MQ=255)

                                                                                                     gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                   <  4:130108‑M1/139‑3 (MQ=255)

                                                                                                          aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  7:96800‑M1/139‑8 (MQ=255)

                                                                                                          aataatTCTATGAGTCGA‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                              <  1:278685‑M1/139‑8 (MQ=255)

                                                                                                            taatTCTATGAGTCGC‑TTTTTTAAATTTTGAAATTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                            <  5:324847‑M1/139‑10 (MQ=255)

                                                                                                                    tgtgtcgC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGATAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  1:230135‑M1/136‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACTTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:237705‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:129758‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCCAGACGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  2:288305‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  4:62626‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  3:271744‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  5:304570‑M1/139‑18 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                       <  1:41293‑M1/136‑15 (MQ=255)

                                                                                                                    tGAGTCGC‑TTTTTTAAATATGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                    <  2:237037‑M1/139‑18 (MQ=255)

                                                                                                                      aGTCGG‑TTTTTTAATTTTGGAAAGTT‑ACAGGTTACTATAGGGAATGGTGATAATTTATTATATATACTCCTGACAGCTTCCAAGAAGCTTAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  3:103070‑M1/139‑20 (MQ=255)

                                                                                                                      aGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  5:354101‑M1/139‑20 (MQ=255)

                                                                                                                       gTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                  <  7:152382‑M1/138‑20 (MQ=255)

                                                                                                                               ttttAAATTTGGAAAGTT‑ACACGTTACTAAAGTCAATGGACATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  5:87434‑M1/137‑26 (MQ=255)

                                                                                                                               ttttAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  3:148841‑M1/137‑26 (MQ=255)

                                                                                                                               ttttAAATTTGGAAAGTT‑ACAAGTTACTAAAGGGAATGGTGATAAATTATTAGATATACTAGTGACATCTTCCAAGAAGCTAATGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat            <  3:318758‑M1/137‑26 (MQ=255)

                                                                                                                                 ttAAATTTGGAAAGTT‑ACACGTTACTTTAGGGATTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgag         <  6:64019‑M1/138‑29 (MQ=255)

                                                                                                                                   aaaTTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCTCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagta       <  7:243186‑M1/138‑31 (MQ=255)

                                                                                                                                        tGGAAAGTT‑ACACGTTACAAAAGGGAATGGACATAAAATATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaa  <  4:220321‑M1/138‑36 (MQ=255)

CAGTTTCATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTT‑ACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTA  >  Exported/3605‑3877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`