BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,750	C→A	5.3%	intergenic (+35/‑121)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,750	0	C	A	5.3%	11398.5 / 61.3	3584	intergenic (+35/‑121)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base C (154/3027); new base A (327/22); total (526/3058)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3987 total aligned reads displayed.

ATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGT  >  Exported/3612‑3893

atcaAGCAATCAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTac                                                                                                                                                 <  3:10013/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                                <  7:236794/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                                <  3:125715/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                                <  5:27424/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                                <  6:247810/139‑1 (MQ=255)

 tcaAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                                <  8:70078/139‑1 (MQ=255)

 acaggCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTaca                                                                                                                                                <  5:99069/135‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                              <  2:149224/139‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                              <  1:8227/139‑1 (MQ=255)

   aaGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACg                                                                                                                                              <  6:176141/139‑1 (MQ=255)

        aTGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACt                                                                                                                                         <  8:196071/139‑1 (MQ=255)

              cacGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTTGAAAGTTTAAAGTTAATaaatg                                                                                                                                    >  8:46974/1‑136 (MQ=255)

               acGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGa                                                                                                                                  <  1:225237/139‑1 (MQ=255)

                cGCCAAAGTAAAGAATTTAATTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGaa                                                                                                                                 <  2:369077/139‑1 (MQ=255)

                    aaaGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGga                                                                                                                             <  6:150045/139‑1 (MQ=255)

                      aGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTTAATTTTAAAGTTACTAAAGGGAATTgag                                                                                                                            >  7:197148/1‑138 (MQ=255)

                        tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                         <  7:309817/139‑1 (MQ=255)

                        tAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                         <  5:222700/139‑1 (MQ=255)

                        tAAACAAATTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                         <  2:286253/139‑1 (MQ=255)

                         aaaCAATTTAAGTACCATTACTTATTAGCAAGTATTGTCTATTTTTAATAGTTATCTATTAATTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATTGAGATa                                                                                                                         <  2:110935/138‑1 (MQ=255)

                         aaaCAATTTAAGGACCATTACTTATGAGCAAGTATTGGCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATa                                                                                                                         <  6:53594/138‑1 (MQ=255)

                          aaCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATaaa                                                                                                                       <  6:336693/139‑1 (MQ=255)

                           aCAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGCGTTTTTTTATTTTGTAAGATTAC‑CGTTTCTAAAAGGAATTgagtgaaa                                                                                                                       >  7:348927/1‑133 (MQ=255)

                            cAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAatt                                                                                                                     <  2:23424/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGTGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  8:111483/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  5:336416/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  5:252794/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  7:145230/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  1:349960/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  3:332509/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  3:262563/139‑1 (MQ=255)

                               tttAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  1:294836/139‑1 (MQ=255)

                                ttAAGTAACATTTCTTTTGAGCTAGTATTGTGTTTTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTACTAAAGGGAATGGAGATAAattatt                                                                                                                  <  6:81147/138‑1 (MQ=255)

                                 tAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑‑TTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                <  3:217818/138‑1 (MQ=255)

                                  aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAg                                                                                                                <  5:200798/138‑1 (MQ=255)

                                  aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTTATTTTGTTAGTTTGACGGTTCTAAATGGTTTGGTGATTAATTTTTTGa                                                                                                               >  3:69843/1‑139 (MQ=255)

                                   aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                              <  1:276809/139‑1 (MQ=255)

                                   aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTAAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                              <  4:175491/139‑1 (MQ=255)

                                       ccATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAtata                                                                                                           <  7:105916/138‑1 (MQ=255)

                                         aTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTAAAATTAAACGGTTATAAAAGTAATTGAGTTAAATTATAAtatataaa                                                                                                         >  4:116767/1‑136 (MQ=255)

                                              ttATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                   <  8:324176/139‑1 (MQ=255)

                                                  gAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGATATAATTCTATGAGTCGC‑GTTTTTTAATTTGGTTAGTTTCCCGTTTCTAAAGGGTATGGTGATTAATTATTTGATTTTCATCTGACAggt                                                                                               >  8:230796/1‑137 (MQ=255)

                                                   aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTATTTTTGAAAGTTTAACGTAACTAAATGGGAAGGAGAGAAATTAATATATATAATACTGACAGCtt                                                                                              >  6:284164/1‑139 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                           <  4:207248/139‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                           <  1:100480/139‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                           <  1:251495/139‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                           <  4:55526/139‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                           <  5:367936/139‑1 (MQ=255)

                                                      aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                           <  3:172051/139‑1 (MQ=255)

                                                       aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGTAAGTTACAAGTTACTAAATGGAATGGAGATAAATTTTTTTATATAATAAAGAAAGCttac                                                                                            >  7:286392/1‑135 (MQ=255)

                                                        gTATTGTCTTTTTTTAATAGTTATCTTTTTTTTAACGGTAGGAAATAATTCTATGTGTCGC‑TTTTTTAAATTTGGAAAGTTTCAAGTTACTAAAGGGATGGGAGATAAATTATTAGATATACTACTGACTGCGTCCaag                                                                                         <  4:352356/139‑1 (MQ=255)

                                                        gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                         <  8:164758/139‑1 (MQ=255)

                                                        gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                         <  2:319795/139‑1 (MQ=255)

                                                        gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TGTTTTTAATTTTGAAATTTTAACGGTACTAAATGGTATGGGGAGTAATTTTTTTATATAGTACTGACAGCTTcc                                                                                            >  8:270780/1‑136 (MQ=255)

                                                        gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTTGAAAGTTACACGGTAATAAATGGAATTGATATAAAATATTATATAAAATACAGAAAAATTCCaa                                                                                          >  6:314234/1‑138 (MQ=255)

                                                         tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                        <  3:145149/139‑1 (MQ=255)

                                                                  tttttATTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                                <  1:148757/138‑1 (MQ=255)

                                                                  tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAaga                                                                               <  6:204427/139‑1 (MQ=255)

                                                                   ttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTATTTTTGTAAGTTTCAAGTGAATAAATGGAATGGAGATAAATTATTATATATAATAATGACAGCTTCCAAGATGCTAAagag                                                                              >  5:294424/1‑139 (MQ=255)

                                                                    tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                              <  5:112585/138‑1 (MQ=255)

                                                                    tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                              <  1:4041/138‑1 (MQ=255)

                                                                    tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                              <  1:313586/138‑1 (MQ=255)

                                                                           gTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                      <  2:259546/139‑1 (MQ=255)

                                                                            ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGGAAGTTTAACGGTTCGTAAGGGGATTGTGATTAATTTTTTTATATACGTCTTACAGCTTACCAGAAGCTAAATAGGTGCCTAgc                                                                     >  3:310943/1‑139 (MQ=255)

                                                                            ttATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAATTTGGTAAGTTTCACCATACTAAAAGGAATTGAGATAAATTATTATATATAATACTGACAGCATCCAAGAAGCTAAACAGGTACCAAgc                                                                     >  5:178876/1‑139 (MQ=255)

                                                                              aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTTTAAGTTTAACGGTTATAAAAGGAATGGTTATAAATTTTTATTTATAAAAAAGACAGCTTCCAACAAGCTAAAAAGGTAACAAgcgc                                                                   >  2:195001/1‑139 (MQ=255)

                                                                              aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTAAAATTTGTAATTTAAACGGTTATAAATGGTATGGTGATTAATTATTTTATATAATAATGACAGCGTACAAGAAGCTAAATAGGTCCCGAtcgc                                                                   >  5:314268/1‑139 (MQ=255)

                                                                                 tattatTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTa                                                                <  8:136083/139‑1 (MQ=255)

                                                                                   ttattTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACg                                                              <  7:123224/139‑1 (MQ=255)

                                                                                      tttAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTTATTTTGTTATTTTAAAGTTTGTAAAAGGTATGGTGATAAATTATTTTATTTAATAATGACAGCGTGCCAGAACCTAAAAAGGGACCGAGCGCCGGCgggg                                                           >  4:31601/1‑139 (MQ=255)

                                                                                          aCGGCATGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGTAAAGTTACACGTTACTTATGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTACCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                        <  5:82109/138‑1 (MQ=255)

                                                                                              gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                   <  1:169429‑M1/139‑3 (MQ=255)

                                                                                              gaggaAATAATTCTATGAGTCGC‑GGTTTTTAATTTGTTAATTTTGGCGGTAGTTAAGGGGATGGAGGTAAATTATTTTATATACGAATTACAGCGTCCCAGAAGCTAAATAGGTCCCGAGCGCCGGCGGGGGAGTTggg                                                   >  4:146211/1‑137 (MQ=255)

                                                                                                ggaAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGAAACTTTCACCTTTCTAAAGGGAATGGAGATAAATTATTATATATAATACAGACAGCTTCCAACAAGCTAAATAGGTCCCTAGCGCCGACGGGGAAttttgggg                                                 >  7:37638/1‑134 (MQ=255)

                                                                                                   aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                              <  1:124732‑M1/139‑8 (MQ=255)

                                                                                                   aataatTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                              <  3:240503‑M1/139‑8 (MQ=255)

                                                                                                          cTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaa                                        <  7:60956‑M1/138‑14 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAGGTTACTAAAGGTAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  6:360490‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGTGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  3:362731‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  7:262543‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  4:90126‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  3:93175‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGAAATACTACTGCCAGCTTCCAAGAAGCAAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  4:284186‑M1/139‑18 (MQ=255)

                                                                                                             tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGACATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  8:353711‑M1/139‑18 (MQ=255)

                                                                                                              gAGTCTC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  3:48894‑M1/138‑18 (MQ=255)

                                                                                                              gAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                    <  7:333356‑M1/138‑18 (MQ=255)

                                                                                                               aGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                  <  7:218400‑M1/139‑20 (MQ=255)

                                                                                                               aGTCCC‑TTTTTCAAATTAGGAAAGTCACACGTTACTCCCCGCCAGGCACATAAATTATAAGATAAACTACTGACAGCTTCCACGAAGCTAAAGCGGCCCCAAGCGCCTACGGGGAATTT‑‑‑‑‑GGGGTACATTGAAAAAGGaa                             <  8:302734/139‑1 (MQ=255)

                                                                                                                gTCGA‑TTTTTTAAATTTTGAAATTTACACGTTACTAAAGGGAATTGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                  <  5:55310‑M1/138‑20 (MQ=255)

                                                                                                                      ttttttAAATTTGGAAAGTTACTCGTTACTAAAGGGAATGGAGATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                            <  2:65805‑M1/139‑26 (MQ=255)

                                                                                                                      ttttctTAATTAGGAAAGTTACACGGAACTAACGGGAAGGGAGATACATTATTAGATAAACTACGGACATCTTCCCCGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                            <  7:174479‑M1/139‑26 (MQ=255)

                                                                                                                        ttttAATTTTGGAATGTTACACGTTACTAAAGGGAATGTAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                            <  6:119832‑M1/137‑26 (MQ=255)

                                                                                                                        ttttAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                            <  5:134923‑M1/137‑26 (MQ=255)

                                                                                                                         tttAAATTTTGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatga                          <  3:292368‑M1/138‑28 (MQ=255)

                                                                                                                                   gAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTCGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaaca                <  5:159889‑M1/138‑38 (MQ=255)

                                                                                                                                          acacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtactttgaaaaaggaagagtatgagtattcaacatttccgtg        >  4:246232‑M1/1‑94 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGGATTGGAGATAAATTTTTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt       <  7:87448‑M1/139‑47 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacgttgaaaaaggaagagtatgagtattcaacatttccgtgt       <  1:199352‑M1/139‑47 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt       <  3:197376‑M1/139‑47 (MQ=255)

                                                                                                                                           cacGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt       <  1:58601‑M1/139‑47 (MQ=255)

ATCAAGCAATGAAACACGCCAAAGTAAACAATTTAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACACGTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGT  >  Exported/3612‑3893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`