BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	3,783	G→T	5.8%	intergenic (+68/‑88)	eryR → / → ampR	/vntifkey=4//vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	3,783	0	G	T	5.8%	12660.9 / 114.4	3925	intergenic (+68/‑88)	eryR/ampR	/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand): ref base G (1109/2464); new base T (267/64); total (1390/2535)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 4029 total aligned reads displayed.

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC  >  Exported/3645‑3925

 aaGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGTTAAATTATTAg                                                                                                                                                <  6:64698/138‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                              <  3:233987/139‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                              <  5:126268/139‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                              <  8:140198/139‑1 (MQ=255)

  aGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGat                                                                                                                                              <  7:355601/139‑1 (MQ=255)

            cTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATTCTACTGa                                                                                                                                    <  1:149252/139‑1 (MQ=255)

            attATGATCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                                                                    <  7:139684/138‑1 (MQ=255)

               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGa                                                                                                                                    <  1:53124/136‑1 (MQ=255)

               aTGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGAc                                                                                                                                   <  2:17188/137‑1 (MQ=255)

                  aGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTGGTAATTTAAAC‑GTTACTAAATGGTATTGAGATAAATTATTATATATACTACAGACAGCt                                                                                                                               >  2:213150/1‑138 (MQ=255)

                     aaGTATTGTCTATTTTTAATATTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                           <  7:235689/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                           <  1:97002/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                           <  4:259977/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                           <  1:251495/139‑1 (MQ=255)

                     aaGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCa                                                                                                                           <  1:100480/139‑1 (MQ=255)

                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                          <  3:155143/139‑1 (MQ=255)

                      aGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGCAGATAAATTATTAGATATACTACTGACAGCTTCCaa                                                                                                                          <  3:353131/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TATTTTAAATTTGGAAAGGTACAC‑GTTACTAACGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                         <  4:4789/139‑1 (MQ=255)

                       gTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑GGTTTTTAATTTTGTAAGTTAAACGGTTAATAAAGGTATTGTGGTTAAA‑TATTATATATAATAAAGACAGcgtac                                                                                                                            >  4:348155/1‑132 (MQ=255)

                       gTATAGTCTATTTATAATAGTTATCTATTATTTAACGGGAGGAAATAATCCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                         <  5:15237/139‑1 (MQ=255)

                        tATTGTCTATTTTTAATAGTTATCTATTATTTAACGGTAGGAAATAATTGTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaag                                                                                                                         <  7:117730/138‑1 (MQ=255)

                         caggTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                        <  3:10766/135‑1 (MQ=255)

                         aTTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCaaga                                                                                                                        <  8:229104/138‑1 (MQ=255)

                             tCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTAAAATTTTGAAAGTTAAAC‑GTTACTAAATGGAATTGAGATAAATTATTAGATATACTAATGACATCTTACAAGAAACt                                                                                                                    >  8:343041/1‑138 (MQ=255)

                                 tttttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAag                                                                                                                <  7:214714/138‑1 (MQ=255)

                                   tttAATATGTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                              <  6:212558/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                              <  1:122664/138‑1 (MQ=255)

                                   tttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAagag                                                                                                              <  7:259352/138‑1 (MQ=255)

                                    ttAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAgg                                                                                                             <  6:185297/138‑1 (MQ=255)

                                      aaTAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTc                                                                                                           <  5:48655/138‑1 (MQ=255)

                                             aTCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTTAATTTGTTAATTTTAAA‑GTTTCTAAAATGAATGGAGGTAAAATATTATATATAATAAAGAAACAATCAAACAAGCTAAAAAGGTCCCTaaagc                                                                                                   >  6:289974/1‑135 (MQ=255)

                                                    attTAACGGGAGGAAATAATTCTATGAGTCTC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                             <  3:318030/138‑1 (MQ=255)

                                                     tttAACGGGAGGAAATAATTCTATTAGTAGC‑TTTTTTAAATTTGGAAATTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                            <  1:225075/138‑1 (MQ=255)

                                                     tttAACGGGAGGAAATAATTCTATGAGGCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACggg                                                                                            <  2:278462/138‑1 (MQ=255)

                                                        aaCGGGAGGAAATAATTCTATGAGTCGC‑GTTTTTTAATTTTGTAATTTTAAC‑ATTAAAAAAAGGAATTGAGATAAAATATTAATTATTATAATAAAAACTACAAAAAAACAAAAAAGGTACCTACCGCCAACCGGGAAt                                                                                        >  2:48247/1‑139 (MQ=255)

                                                         aCGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAAt                                                                                        <  8:6559/138‑1 (MQ=255)

                                                           gggAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTG                                                                                     <  7:95352‑M1/139‑1 (MQ=255)

                                                             gagtaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgt                                                                                   <  6:93818‑M1/139‑3 (MQ=255)

                                                             gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                   <  1:169429‑M1/139‑3 (MQ=255)

                                                             gaggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgg                                                                                   <  8:11859‑M1/139‑3 (MQ=255)

                                                              aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                  <  5:104003‑M1/139‑4 (MQ=255)

                                                              aggaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                  <  1:346424‑M1/139‑4 (MQ=255)

                                                              aggaAATAATTCTATGAGTCGA‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGGTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggg                                                                                  <  4:164452‑M1/139‑4 (MQ=255)

                                                               ggaAATAATTCTATGAGTCGCGGTTTTTAATTTTGGAATG‑TAAAC‑CTGTAAAAAGGGAAAGGGGTTAAAATATTATATTTTATACTGCCAGCGTCCAAACAGCGAAAAAAGTACCGAGCGCCGACGGGGGAATTggggg                                                                                 >  6:101804/1‑135 (MQ=255)

                                                                gaAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggta                                                                                <  2:308588‑M1/139‑6 (MQ=255)

                                                                         cTATGATTCCA‑TTTTTAAAATTTGGAAAGTTACAC‑GTTACTAAAGGTAATGGAGATAATTTATTAGATATACTAGTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGtggtacattgaaa                                                                        <  5:27422‑M1/138‑14 (MQ=255)

                                                                            tGAGTCTC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  1:299581‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGTAAAGTTACAC‑GTTACTAAAGGGAATTGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAACCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  8:165193‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGATATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaa                                                                       <  1:202305‑M1/136‑15 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  7:43264‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  5:176550‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  6:323980‑M1/139‑18 (MQ=255)

                                                                            tGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  7:167463‑M1/139‑18 (MQ=255)

                                                                            tGAGGCGC‑TTTTTTAAATTTTGAAAGTTACAC‑GTTAATAAATGGTATGGTGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  2:24612‑M1/139‑18 (MQ=255)

                                                                             gAGTGGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGTAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  7:333059‑M1/138‑18 (MQ=255)

                                                                              aGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                  <  4:102688‑M1/139‑20 (MQ=255)

                                                                               gTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACCGCTTCCAAGAAGCTAAAGAAGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaa                                                                  <  3:208124‑M1/138‑20 (MQ=255)

                                                                                tCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTTCTAACGGGAATGTAGATAAATTATTAGATATAGTACTGACTTCTTCCAATAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaaga                                                                <  7:192694‑M1/139‑22 (MQ=255)

                                                                                      tttttAAATTTGTAAAGTTACAC‑TTTAATAAAGGGAATGTAGATAAATTATAAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  3:85730‑M1/138‑26 (MQ=255)

                                                                                      tttttAAATTTGGAAAGTTACAC‑TTTAATAAAGGTAATGTATATAAATTATTATATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  5:363791‑M1/138‑26 (MQ=255)

                                                                                       ttttAAATTTGTAAAGTTACAC‑TTTACTAAATGGAATGTATATAATTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  2:133593‑M1/137‑26 (MQ=255)

                                                                                       ttttAAATTTGGAAATTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  3:64985‑M1/137‑26 (MQ=255)

                                                                                            aaTTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                     <  5:78991‑M1/139‑33 (MQ=255)

                                                                                                                 aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGggttacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                 >  8:369778‑M1/1‑87 (MQ=255)

                                                                                                                 aCTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                       <  8:292113‑M1/133‑47 (MQ=255)

                                                                                                                  cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                 >  5:22005‑M1/1‑86 (MQ=255)

                                                                                                                  cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                 >  5:195505‑M1/1‑86 (MQ=255)

                                                                                                                  cTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccct                                 >  6:2599‑M1/1‑86 (MQ=255)

                                                                                                                        ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGCGGTCCCTAGCGCCTACGGGGAAGTTGggggacattgaaaaaggaagagtatgagtattaaacatttccgtgtcgccctgattaca                          >  6:119691‑M1/1‑80 (MQ=255)

                                                                                                                        ggAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    >  8:321304‑M1/1‑80 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  3:179703‑M1/1‑78 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattccctt                        >  4:134831‑M1/1‑78 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcg                                     >  6:370793‑M1/1‑78 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgt                                       <  8:200895‑M1/124‑47 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                     >  4:227221‑M1/1‑78 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    >  7:283782‑M1/1‑78 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtaca                                                                              >  3:275018‑M1/1‑78 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtac                                                                               <  1:72891‑M1/84‑7 (MQ=255)

                                                                                                                          aaTGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTACCTAGCGCCTACGCGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcacttattccctt                        >  2:82134‑M1/1‑78 (MQ=255)

                                                                                                                            tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAg                                                                                                      <  3:40685/59‑1 (MQ=255)

                                                                                                                            tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACgg                                                                                             <  8:264978/68‑1 (MQ=255)

                                                                                                                            tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtatt                                                     >  3:133403‑M1/1‑76 (MQ=255)

                                                                                                                            tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            >  1:32227‑M1/1‑76 (MQ=255)

                                                                                                                            tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtat                                                            <  4:19183‑M1/101‑26 (MQ=255)

                                                                                                                            tGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    >  1:278040‑M1/1‑76 (MQ=255)

                                                                                                                             ggAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaagg                                                                    <  5:251963‑M1/92‑18 (MQ=255)

                                                                                                                               agaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg                   >  3:252557‑M1/1‑73 (MQ=255)

                                                                                                                                gaTATATTATTAGATATACTACTGACTGCTTCCAACACGCTAACGAGGTCCCTAGCGCCTACGGGGAATTTGtggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttg                   <  7:311329‑M1/138‑67 (MQ=255)

                                                                                                                                gaTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgc                  <  1:367932‑M1/139‑68 (MQ=255)

                                                                                                                                 attaattaTTAGATATACTACTGACAGCTTCCAAGAAGCAAAAGAGGTCCCTAGCGCCTACGGGGAATTTGtggtacattgaaaaaggaagagtatgagtattcaccctttcgttgtcgcacttagtcccttttttgct                 >  4:315650‑M1/4‑71 (MQ=255)

                                                                                                                                 aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg                 >  5:340304‑M1/1‑71 (MQ=255)

                                                                                                                                 aTAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttatt                             >  2:345945‑M1/1‑71 (MQ=255)

                                                                                                                                   aaaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacatt                                                                            <  4:77649‑M1/78‑10 (MQ=255)

                                                                                                                                    aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggca              >  6:348290‑M1/1‑68 (MQ=255)

                                                                                                                                    aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttagtccctagttggcggct              >  5:31642‑M1/1‑68 (MQ=255)

                                                                                                                                    aaTTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacattgccgtgtcgcccttattcccttttttgcggca              >  7:64772‑M1/1‑68 (MQ=255)

                                                                                                                                     attattAGATATACTACTGACAGCTTCCAAGAAGCTGAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcg                 <  3:301753‑M1/135‑69 (MQ=255)

                                                                                                                                     attattAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat             <  5:294566‑M1/139‑73 (MQ=255)

                                                                                                                                         ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg         >  4:287014‑M1/1‑63 (MQ=255)

                                                                                                                                         ttaGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGgggtacattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttg         >  6:150940‑M1/1‑63 (MQ=255)

TAAGTACCATTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTATTTAACGGGAGGAAATAATTCTATGAGTCGC‑TTTTTTAAATTTGGAAAGTTACAC‑GTTACTAAAGGGAATGGAGATAAATTATTAGATATACTACTGACAGCTTCCAAGAAGCTAAAGAGGTCCCTAGCGCCTACGGGGAATTTGTATCGGGGTACATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCC  >  Exported/3645‑3925

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`