BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	4,625	A→C	5.9%	K252T (AAG→ACG)	ampR →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	4,625	0	A	C	5.9%	9702.3 / 95.1	3604	K252T (AAG→ACG)	ampR	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (1999/1296); new base C (37/251); total (2047/1557)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 3659 total aligned reads displayed.

TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  Exported/4487‑4763

  gACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  5:103807/138‑1 (MQ=255)

   acgggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTGCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  5:48432/136‑1 (MQ=255)

   aCTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  6:373476/139‑1 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  4:367297/1‑139 (MQ=255)

    cTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGccc                                                                                                                                        >  3:73829/1‑139 (MQ=255)

    cTGGATGGAGGAGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTTGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGACAGATGGTAAGccc                                                                                                                                        >  6:281133/1‑139 (MQ=255)

     tggatggaGGCGGATAAAGTTGCAGGACCACTTCTGCTCTCGGCCCTTCCGGCTGGATGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATTGTAAGCCCt                                                                                                                                       >  3:117917/1‑139 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAACTCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     <  7:146593/139‑1 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     >  1:310196/1‑139 (MQ=255)

       gatggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCGGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGGCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTcc                                                                                                                                     <  1:100469/139‑1 (MQ=255)

       gagggaGGCGGATAAAGTTGCAGGACCACTTCTGCGAGCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      <  8:247034/135‑1 (MQ=255)

        atggaTGCGGATAAATTTTCAGGACCACTTCTGCGATAGGCAGTTCAGGCTGGCTTGATTATTGCTTATAAATCTTGAGCCGGTGAGCGTGGGTCTCGAGGAAACATTGCAGCACTTTGGCAAGATGTTAAGCCCTccc                                                                                                                                    <  1:250283/139‑1 (MQ=255)

        atggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  6:35161/134‑1 (MQ=255)

        atggaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTccc                                                                                                                                    <  8:59583/139‑1 (MQ=255)

          ggaGGGTGATAAAGTTGCAGGACCACTTCTGCGCTCGGCGCTTCCGGCGGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCg                                                                                                                                   <  6:124794/138‑1 (MQ=255)

           gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 <  5:358188/139‑1 (MQ=255)

           gaGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 <  5:76397/139‑1 (MQ=255)

            aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTTGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTAt                                                                                                                                <  2:32762/139‑1 (MQ=255)

            aGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTACGCCCTCCCGTAt                                                                                                                                >  8:292007/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  2:93577/125‑1 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  8:315149/1‑128 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  5:14702/1‑136 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  3:36996/1‑139 (MQ=255)

                 gATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAg                                                                                                                           >  3:260679/1‑139 (MQ=255)

                   tAAAGTTGCAGGACCACTTCTGCGCTCGGCCCGTCCGGCGGGCTGGGTTATTGCTGATAAAGCTGGCGCCGGTGAGCGTGGGGCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGtt                                                                                                                         <  5:26018/139‑1 (MQ=255)

                     aaGGTGCAGGACCCCTTCTGCGCGCGGCCCTTCCGGGTGGCTGGTTTATTGCTGAAAAATCTGGAGCCGGGGAGCGTGGGTCTCGCGGTATCATTGCACCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTAt                                                                                                                       <  3:293221/139‑1 (MQ=255)

                      aGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATc                                                                                                                      >  3:205234/1‑139 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  2:169500/1‑118 (MQ=255)

                           cAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTacac                                                                                                                 >  7:349730/1‑139 (MQ=255)

                                ccACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGCGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTCTCGTAGTTATCTACACGACgg                                                                                                            >  1:368455/1‑139 (MQ=255)

                                ccACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACgg                                                                                                            >  6:305417/1‑139 (MQ=255)

                                  aCTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGTCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                           >  6:25828/1‑138 (MQ=255)

                                   cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                           >  3:229294/1‑137 (MQ=255)

                                   cTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGAACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACggg                                                                                                           >  7:198729/1‑137 (MQ=255)

                                      cTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTa                                                                                                                            <  5:95153/117‑1 (MQ=255)

                                        gcgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCAGTATCGTAGTTATCTACACGACGGGGAGTCAg                                                                                                    >  8:194227/1‑139 (MQ=255)

                                         cgcTCGGCCCTTCCGGCTGGGTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCGACCCGTATCGTAGTTAGCTGCCCGAGCGGGATTCAgg                                                                                                   >  5:280832/1‑139 (MQ=255)

                                         cgcTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAgg                                                                                                   >  5:174156/1‑139 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  3:138315/1‑96 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  7:320002/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  4:6898/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  4:220061/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  3:32372/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAAc                                                                                               >  8:220665/1‑138 (MQ=255)

                                              ggCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACt                                                                                              >  8:268627/1‑139 (MQ=255)

                                                   ttCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGAGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGCGGAGTCAGGCAACTATGGa                                                                                         >  4:111829/1‑139 (MQ=255)

                                                     ccGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTc                                                                                                                                      >  6:217267/1‑92 (MQ=255)

                                                      gagcgggctgtTTTTTTGCTTAAAAATGTGGAGCCGGTGTGGGGGGGTCGCGGGGTATCATTGCTGCCGGGGGGCCAGTTGTTAAGCCCTCCCGTTTCTTTGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  6:76780/134‑1 (MQ=255)

                                                      cGGGTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  7:62232/139‑1 (MQ=255)

                                                      cGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGa                                                                                      <  7:46149/139‑1 (MQ=255)

                                                       actggtctggTTTTTTGCTGATAATTCGGGTGCCGGTGTGGGGGGGTCTCGCGGTATCTTTGCAGCCCTGGGGCCAGTGGTTAAGCCCTCCCGTATGGTAGTTATAAACACGACGGGGAGTCAGGCAACTATGGATGaa                                                                                     <  4:250424/133‑1 (MQ=255)

                                                        gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTa                                                                                                                                 >  7:248964/1‑94 (MQ=255)

                                                        gctggctgGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAAc                                                                                    >  7:118184/1‑139 (MQ=255)

                                                            gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         >  8:80436/1‑82 (MQ=255)

                                                            gctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGaaa                                                                                >  6:15244/1‑139 (MQ=255)

                                                             ctggTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCATCTATGGATGAACGtaa                                                                                >  1:54713/1‑138 (MQ=255)

                                                                gTTTTTTGCTTATAATGGTGGAGCCGGGGAGGGGGGGGCGCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCGGTATCTTATTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                            <  2:264622/139‑1 (MQ=255)

                                                                      tGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGa                                                                            >  8:165777/1‑133 (MQ=255)

                                                                       gCTTATAAAGCTGGCGCGGGTGCGCGGGGGTCTCGCGGTATCATGGCAGCACGGGGGCCATAGGGTAAGCCCCCCCGTAGCTTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATc                                                                      <  1:222449/138‑1 (MQ=255)

                                                                       gCTCATAAATCTGGAGCCGGGGCGCGGGGGGCGCGCGGTATCATTGCAGCCCGGGGCCCAGATGGAAAGCCCCCCCGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATc                                                                      <  6:329915/138‑1 (MQ=255)

                                                                           aTAAATCTGGCGCGGGTGGGCGTGGGGCGGGCGGTATCATTGCCGCACGGGGGCCAGATGGAAACCCCTCCCTTATCTTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCt                                                                   <  7:84828/137‑1 (MQ=255)

                                                                           aTAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGcc                                                                                                                                         <  8:45195/67‑1 (MQ=255)

                                                                                       ccGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCt                                                      >  8:346458/1‑138 (MQ=255)

                                                                                        gggTGCGCGTGGGGCGCGCGGTATCATGGCCGCACTGGGGCCAGATGGTACGCCCTCCCGTAGCGTAGTTAACACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  8:128512/138‑1 (MQ=255)

                                                                                        cGGTGCGGGTGGGTCTCGCTGTATCATTCCAGCACTGGGGCCAGATGTTACGCCCTCCCGTATCGTAGTTATATCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  2:71044/139‑1 (MQ=255)

                                                                                        cGGTGCGCGGGGGTCTCGCGGTATCATTGCAGCACGGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  3:164816/139‑1 (MQ=255)

                                                                                        cGGGGAGCGTGGGTCTCGCGGAATCTTTGCAGAACTGGGTCCAGTTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCa                                                    <  6:137463/139‑1 (MQ=255)

                                                                                          gTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAg                                                                                                                                           <  7:271924/50‑1 (MQ=255)

                                                                                              gggggggTCTCGTGGTATCATTCCCGCCCGGGGGCCTGATGGTACGCCCGCCGGTATCGTAGTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                              <  7:174663/135‑1 (MQ=255)

                                                                                              gCGGGGGTCGGGCGTTATCTTTGAAGCTCGGGGGCCATTTGTTAAGCCCTCCCTTATGTTTTTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                              <  4:342973/139‑1 (MQ=255)

                                                                                               cGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGAtt                                              >  4:173153/1‑138 (MQ=255)

                                                                                                    gTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCAt                                        >  7:223805/1‑139 (MQ=255)

                                                                                                         gcgGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGt                                    >  1:34123/1‑138 (MQ=255)

                                                                                                              aTCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGt                              >  1:163792/1‑139 (MQ=255)

                                                                                                               tCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCg                                                                                                                              >  2:363667/1‑42 (MQ=255)

                                                                                                                    ggcgcaCGGGGGCCATTTGGTAAGCCCGCCCGTATCGTATTTATCACCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         <  4:278873/135‑1 (MQ=255)

                                                                                                                    gcagcaCTGGGGCCTGTTGGAAACCCCACCCGTTTGGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAc                         <  2:196080/138‑1 (MQ=255)

                                                                                                                     cagcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGAcc                        >  1:134284/1‑138 (MQ=255)

                                                                                                                       gcaCTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAg                     >  3:346142/1‑139 (MQ=255)

                                                                                                                         acgggggCCAGATGGTAACCCCTCCCGTATCGTAGTAATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   <  4:137252/136‑1 (MQ=255)

                                                                                                                           tGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 >  5:306247/1‑139 (MQ=255)

                                                                                                                           gggggCCATCTGGAAACCCCTCCCGTAGCGTCCAAAAAAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGtt                   <  2:135085/136‑1 (MQ=255)

                                                                                                                           ggggcCCTGATGGTAAGCCCTCCCGTAGCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  1:7329/138‑1 (MQ=255)

                                                                                                                           gggcgCCAGATGGTAAGCCCACCCGTATCGTAGTTAACTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  4:31385/135‑1 (MQ=255)

                                                                                                                             gggCCAGTTGGTCAGCCCACCCGTATCTTAGTTATCAACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTa                 <  7:100643/137‑1 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTGTCTACACGACGGGGAGTCAGGCAGCGATGGAGGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATGAGGCATTGGTCACTGTCAGACCTAGtgtcgt               >  6:18131/1‑134 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTCGCTACCCGACGGGGAGTCCGGCAACTAGGGATGACCGAAATAGCCAGATCGCTGAGATAGGTGCCTCACTGATTACGCATTGGTAACTGTCCGACCAAGTGTACt               >  2:4978/1‑139 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGCCCAAGTTTACt               >  1:279099/1‑139 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  1:95764/1‑139 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACt               >  1:1884/1‑139 (MQ=255)

                                                                                                                             gggCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGAATTGGTAACTGTCAGACCAAGTTTAc                >  4:192159/1‑138 (MQ=255)

                                                                                                                                  acaTGGTAAGCACTACCGTAAAGTAGTTATCTACCCGACGAGGAGTCAGGCAACTATGGATGAATGAAATACACAGATAGATCAGATACGTGCCTCACTCATTAATCAGAGGAAACTGTCAGACCATGCTTACTCata           >  2:294600/3‑138 (MQ=255)

                                                                                                                                  aGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatat          <  5:131930/139‑1 (MQ=255)

                                                                                                                                    aTGTTAAGCCCTCCTTTATCTTAGTTATCTCCACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTTATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         <  8:106018/138‑1 (MQ=255)

                                                                                                                                    aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  5:23240/1‑138 (MQ=255)

                                                                                                                                    aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  5:207661/1‑138 (MQ=255)

                                                                                                                                    aTGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCatata         >  3:180729/1‑138 (MQ=255)

                                                                                                                                        tAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  5:335352/1‑139 (MQ=255)

                                                                                                                                         aaGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACtt    >  2:235746/1‑138 (MQ=255)

                                                                                                                                          aGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTa  >  7:298764/1‑139 (MQ=255)

TAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTA  >  Exported/4487‑4763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`