Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 5,464 T→G 5.1% intergenic (+733/‑424) ampR → / → lacZalpha /vntifkey=4//vntifkey=4

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported5,4640TG 5.1% 12418.5 / 102.2 5634intergenic (+733/‑424)ampR/lacZalpha/vntifkey=4//vntifkey=4
Reads supporting (aligned to +/- strand):  ref base T (2629/2557);  new base G (175/221);  total (2851/2783)
Fisher's exact test for biased strand distribution p-value = 1.42e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5668 total aligned reads displayed.

AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT  >  Exported/5326‑5601
                                                                                                                                           |                                                                                                                                         
              tCCGGTAAGCGGCAGGGTCGGAACAGTAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCg                                                                                                                              <  3:157427/138‑1 (MQ=255)
              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGCTGC‑TCGTCAGGGGGGCgg                                                                                                                             >  7:48230/1‑139 (MQ=255)
              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCg                                                                                                                              <  3:98614/138‑1 (MQ=255)
              tCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGGTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTTATTC‑ACCTCAAGGGGGccg                                                                                                                             >  3:4423/1‑137 (MQ=255)
              gccGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCg                                                                                                                              <  3:177801/137‑1 (MQ=255)
                          cAGGGTCGGAACAGGAGTCCGCACGTGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTTACTTTAGCGTCGATTTTTGTGATGG‑TCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                                 <  5:220489/139‑1 (MQ=255)
                                 ggAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGc                                                                                                            >  5:146231/1‑137 (MQ=255)
                                  gAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAg                                                                                                         >  8:117364/1‑139 (MQ=255)
                                        gagcGCGCACGCGGGAGGTTCCTGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCTGGGGGGCGGAGCCTATGTAAAAACGCCAGCAAcgc                                                                                                   <  4:78848/139‑1 (MQ=255)
                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCATGGTGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                   >  8:170694/1‑139 (MQ=255)
                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCg                                                                                                                              >  1:313063/1‑112 (MQ=255)
                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                   <  1:40693/139‑1 (MQ=255)
                                        gagaGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑ACGTCAGGGGGGCg                                                                                                                              >  4:279062/1‑112 (MQ=255)
                                          gagCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAATGCgg                                                                                                 <  7:204817/139‑1 (MQ=255)
                                          gagCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                 <  6:335633/139‑1 (MQ=255)
                                           ggcgcCCGCGGGAGCTTCCCGGGGGAACCGCCTGGTATCTTTATAGTCCTGGCGGGTGTCCCCACCTCTGACTTGCGCGTCGATTTTTGTGAGGC‑TCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGc                                                                                                <  5:54446/138‑1 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTTGTTC‑TCTTCAGGGGGGCGGAGCCGATTGAAAAACGCCAGCAACGCGGccg                                                                                              >  4:149741/1‑138 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGG‑TCGTCAGGGGGGGGGAGCCTATGGAAAACCGCCAGCCACGCGGCCt                                                                                              >  1:288531/1‑139 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TGGTCAGGGGGGCGGAGCCTATTGAAAAACGCCAGCAACGCGGccg                                                                                              >  8:353943/1‑138 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAgggggg                                                                                                                                >  7:72634/1‑105 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCg                                                                                                                              <  2:52959/107‑1 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                              >  2:290708/1‑139 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCt                                                                                              >  6:363096/1‑139 (MQ=255)
                                             cgcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCAACTCTGACTTGAGCGTCGATTTTTGTGATGCATGTTCAGGTGGCCGG‑GCGAATTGAAAAACTACAGCAACCCCGCCt                                                                                              >  1:161288/1‑139 (MQ=255)
                                                aCGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCtttt                                                                                           >  4:306782/1‑139 (MQ=255)
                                                aCGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGGGGTGG‑TGGGGAGGGGGGGGGCGCCGATGGAAAAACCCCAGCCACGCGGccggtt                                                                                           >  1:346110/1‑135 (MQ=255)
                                                  gAGGTAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                         <  1:10008/139‑1 (MQ=255)
                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                         <  1:70495/139‑1 (MQ=255)
                                                  gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGACGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGCTGA‑TCGTCAGGGGGGCGGAGCCGATGGAAAAACGCCAGCAACGCGGCCttattt                                                                                         >  6:156080/1‑138 (MQ=255)
                                                       aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGtt                                                                                    >  6:279347/1‑139 (MQ=255)
                                                         cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc                                                                                  >  1:217901/1‑139 (MQ=255)
                                                         cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGATcc                                                                                  >  6:181042/1‑139 (MQ=255)
                                                         cTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCACTGACTTGAGCGTCGATTTTTGTGATGC‑AAGTCAGGGGGGCGGAGCCGTTGGAAAAACGCCAACAACCCGCCCTTTTTACGGttac                                                                                  >  6:209656/1‑137 (MQ=255)
                                                              cggggtAAACGCCGGGTATATTTATAGGCCGGTCGGGTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGAGGC‑TGGTCTGGGGGGCGGAGCCTATGTAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                             <  6:24843/138‑1 (MQ=255)
                                                              cgggggAAACGCCTGGTATCTTTATAGTCCGGGCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGAGGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcc                                                                             <  7:156073/138‑1 (MQ=255)
                                                                ggggAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGCGCCTATGGAAAAACGCCAGCAAACCGGCCTTTTTACGGTTCctggcctt                                                                           >  5:101674/1‑139 (MQ=255)
                                                                    aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                   >  7:232778/1‑111 (MQ=255)
                                                                    aaaCGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgc                                                                       >  8:14546/1‑139 (MQ=255)
                                                                            ggTATCTTTATCGGCCTGGGGGGTTGCGCCCCCGCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGGCCGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt                                                                <  3:215013/138‑1 (MQ=255)
                                                                              tATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctagccttttg                                                             <  6:10484/139‑1 (MQ=255)
                                                                              agtCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                             <  3:267608/137‑1 (MQ=255)
                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATTC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGCCTTTTTAAGGTTCCTGGCCGTTTGCTGGGCTTTTGCTc                                                          >  4:331530/1‑138 (MQ=255)
                                                                                  tttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                        <  7:360341/108‑1 (MQ=255)
                                                                                   ttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTTATGC‑TCGTCAGGGGGGCGGAGCCAATGGAAAAAAGCCAGCAACGCCGCCTTTTTTCGCTTCCTtgccgtttgctggccttttgctaa                                                         >  2:242898/1‑136 (MQ=255)
                                                                                    tataGTCCTGTCGGGTTTCGCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                        >  6:85445‑M2/1‑137 (MQ=255)
                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggccttttg                                                             <  5:122658/133‑1 (MQ=255)
                                                                                    tataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                        >  7:180780‑M2/1‑137 (MQ=255)
                                                                                    tataGTCCTGTCGGGTTTCGCCACCGCTGACTTGAGCGGCGATTTTTGGGATGC‑TCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                        >  3:286768‑M2/1‑137 (MQ=255)
                                                                                    tataGTCCGGTCGGGGTTCGCCACCTCTGACTTGAGCGGCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCAc                                                        >  1:192701‑M2/1‑137 (MQ=255)
                                                                                       aGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATTA‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACCCCACCAAACCCGCCTTTTTAAAGTTACTCGCGATTTTGTGGCCTTTTTGTCACAAGt                                                    >  2:124721/1‑139 (MQ=255)
                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATTC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAAGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTGATATGtt                                                   >  5:171666/1‑139 (MQ=255)
                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGaaa                                                                                                                 <  3:134374/77‑1 (MQ=255)
                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                   >  1:244341/1‑139 (MQ=255)
                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                   <  7:332388/139‑1 (MQ=255)
                                                                                        gTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGtt                                                   >  5:369167/1‑139 (MQ=255)
                                                                                         tCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTc                                                  >  8:73758/1‑139 (MQ=255)
                                                                                           cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACCCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCtt                                                >  1:26605/1‑139 (MQ=255)
                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTTCGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCttt                                               >  1:9445/1‑139 (MQ=255)
                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                        >  8:41966/1‑98 (MQ=255)
                                                                                            tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCttt                                               >  5:337969/1‑139 (MQ=255)
                                                                                             gTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                              <  8:24721/139‑1 (MQ=255)
                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATTG‑GCGGCAGGGGGGGGGAGCCGATGGAAAAACCCCCGCAACCCGGCCGTTTTTAGGTTACGGGGCGTTTGGGGGCCGTTTTGTGACAAGTTGtttg                                              >  4:194546/1‑137 (MQ=255)
                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGG‑GGGTCAGGGGGGGGGGGCCTATGGAAAAACGCCAGCCACGCGGCCGTTTTACGGTTGCGGGCCTTTTGGTGGGCCTTTGGTCACATGGTCTTTcc                                             >  4:122971/1‑139 (MQ=255)
                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCCGCAACGCGGGCTTTTTACGGTTCCTGGCCTTTTGCTGGCCGTTTGGTCAGATGTTCTTTcc                                             >  3:310820/1‑139 (MQ=255)
                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAAcgc                                                                                                   >  7:131038/1‑85 (MQ=255)
                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTa                                                                                         <  2:21156/95‑1 (MQ=255)
                                                                                              tCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTc                                              <  5:313625/138‑1 (MQ=255)
                                                                                               cGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCt                                            <  7:30796/139‑1 (MQ=255)
                                                                                                   tttCGCCACCTCGGACTTGAGCGTCGATTTTTGTGTTGC‑GCGTCTGGGGGGCGGAGCCTTTGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGt                                        <  3:7526/139‑1 (MQ=255)
                                                                                                      cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                      <  5:259121/138‑1 (MQ=255)
                                                                                                         cACCTCTGACTTTAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATc                                    <  8:27624/137‑1 (MQ=255)
                                                                                                            ctctGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTc                                                          <  7:111591/112‑1 (MQ=255)
                                                                                                            ctctGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCt                                >  3:108864/1‑138 (MQ=255)
                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGTGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCt                          <  8:300757/139‑1 (MQ=255)
                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                            <  3:245134/137‑1 (MQ=255)
                                                                                                                 aCTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt                            <  3:332170/137‑1 (MQ=255)
                                                                                                                 aCTTGAGCGTCGATTTTTGGGTTGC‑TCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCt                          <  5:28776/139‑1 (MQ=255)
                                                                                                                      aGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGGCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCGGATTTTGTGGa                     >  6:311963/1‑139 (MQ=255)
                                                                                                                      aGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa                                      <  8:19358/122‑1 (MQ=255)
                                                                                                                      aGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGa                     >  5:82151/1‑139 (MQ=255)
                                                                                                                       gggTCGATTTTTGTGATGC‑TCGTCCGGGGGGCGGAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  5:19177/137‑1 (MQ=255)
                                                                                                                       gcggcgATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  2:368050/139‑1 (MQ=255)
                                                                                                                       gCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  6:127563/139‑1 (MQ=255)
                                                                                                                       gCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  4:170005/139‑1 (MQ=255)
                                                                                                                       gCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  4:231988/139‑1 (MQ=255)
                                                                                                                        cGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt                    <  2:325539/138‑1 (MQ=255)
                                                                                                                         gTCGCTTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCACCGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTTGATaa                  <  4:35601/139‑1 (MQ=255)
                                                                                                                         gTCGATTTTTGTGATGA‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                  <  8:22162/139‑1 (MQ=255)
                                                                                                                         gTCGATTTTGGTGATGC‑GCGTCAGGGGGGCGGTGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                  <  2:168219/139‑1 (MQ=255)
                                                                                                                           cGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACCCCGCCATTTTACGGTTCCTGGCCGTTTGCTCGCCTTTTTCTCACATTTTATTTCCTGCGTTATACCCTGATTCTGTGGATAAcc                >  7:280528/1‑139 (MQ=255)
                                                                                                                                  tgtgATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  4:47032/138‑1 (MQ=255)
                                                                                                                                  tgtgATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  2:26605/138‑1 (MQ=255)
                                                                                                                                  tgtgATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  3:356502/138‑1 (MQ=255)
                                                                                                                                  tgtgAGGC‑GCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  6:57903/138‑1 (MQ=255)
                                                                                                                                  tgggATGC‑TCGGCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTa          <  8:32695/135‑1 (MQ=255)
                                                                                                                                   gtgATGC‑GCGTCAGGGGGGCGTAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTAc         <  2:82993/138‑1 (MQ=255)
                                                                                                                                      aTGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTAc                                                                                        >  4:256205/1‑56 (MQ=255)
                                                                                                                                      aTGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa                  <  7:93532/126‑1 (MQ=255)
                                                                                                                                       tGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCgg                                                                                                 >  7:257250/1‑46 (MQ=255)
                                                                                                                                        gC‑TCGTCTGGGGGGCGGAGCCTAGGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCtt   <  1:128715/139‑1 (MQ=255)
                                                                                                                                           |                                                                                                                                         
AAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGC‑TCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTT  >  Exported/5326‑5601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑