BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,012	A→C	7.1%	Q42P (CAA→CCA)	lacZalpha →	/vntifkey=4

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,012	0	A	C	7.1%	13424.3 / 180.2	5082	Q42P (CAA→CCA)	lacZalpha	/vntifkey=4
Reads supporting (aligned to +/- strand): ref base A (2223/2402); new base C (443/2); total (2676/2406)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5105 total aligned reads displayed.

CACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTG  >  Exported/5874‑6151

   aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGATACACAACt                                                                                                                                          >  4:109088/1‑139 (MQ=255)

   aGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGGTTTACAACGTCGTGACTGGGGAAACCCGGGCGTGACCCCAca                                                                                                                                          >  5:51873/1‑138 (MQ=255)

     gAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCCAcat                                                                                                                                         >  3:281047/1‑136 (MQ=255)

     gAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACtt                                                                                                                                         >  7:111800/1‑138 (MQ=255)

       aaCAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAAt                                                                                                                                      <  2:316279/139‑1 (MQ=255)

         cAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCg                                                                                                                                    <  4:254402/139‑1 (MQ=255)

               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                              >  6:91174/1‑139 (MQ=255)

               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgc                                                                                                                              >  1:128760/1‑139 (MQ=255)

               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                               <  1:219803/138‑1 (MQ=255)

               tGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTg                                                                                                                               <  5:11719/138‑1 (MQ=255)

                 aCCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACATAAAAGCCATgccg                                                                                                                            >  2:284061/1‑137 (MQ=255)

                   cATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTgcagca                                                                                                                          <  8:372097/139‑1 (MQ=255)

                     tGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACATAATCGCCGTGCCGcac                                                                                                                         >  7:144673/1‑138 (MQ=255)

                      gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTTCAACGTCGTGCCTGGGGAAACCCTGGCGGTTCCCAACTTAAAAGCCGTgcagccact                                                                                                                       >  1:281488/1‑135 (MQ=255)

                      gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCATGCAGCACAt                                                                                                                       >  2:2602/1‑139 (MQ=255)

                      gATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCCTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTAACCAACATAATCGCCATTCAGcacaa                                                                                                                       >  8:78893/1‑138 (MQ=255)

                       aTTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATc                                                                                                                      <  7:291466/139‑1 (MQ=255)

                        gtACGCCAAGCTTGCTTTCCTGCTGGTCGACTCTAGAGGATCCCCGGGGACCGAGCTCGAATTCACTGGCCGTCGTTATACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATAGACTTGCAGCACAtg                                                                                                                      <  5:341960/137‑2 (MQ=255)

                                aGCTTGCATTCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                             >  7:343827/1‑139 (MQ=255)

                                aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCTCTGGCCGTCGTTTTACAACGTCGTGACGGGGAAAACCCTGGCCTGTCCCCACTTAATCCCCTTTCAGCACATcccccagtcg                                                                                                             >  2:107752/1‑134 (MQ=255)

                                aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGCAAACCCGAGCGTTACCCCACGTTATAGCCTTGCAGCCCCTGCCCCGTTCg                                                                                                             >  6:70180/1‑139 (MQ=255)

                                aGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCCGCCCCTCCCCCTTTCg                                                                                                             >  1:37384/1‑139 (MQ=255)

                                 gCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGGAAACCCAGGCGTTTCCCAACTTAATCGCCTTGCAGCACAACCCCCTTTCGc                                                                                                            >  6:345045/1‑139 (MQ=255)

                                      cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                        <  8:243815/138‑1 (MQ=255)

                                      cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                        <  7:56590/138‑1 (MQ=255)

                                      cATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGGTACCCAACATAAACGCCTTGCAGCACAAACCCCATTCGCCAGCt                                                                                                       >  8:265341/1‑139 (MQ=255)

                                          ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                   <  2:215017/139‑1 (MQ=255)

                                          ccTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGGTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCg                                                                                                   >  4:92626/1‑139 (MQ=255)

                                            tGCAGGTCGACTCTAGAGGATCCCCGGGTCCCGAGCTCGCTTTGACTGGCCGTCGTTTTACACCGTGGGGGCTGGGGAAACCCTGGGGGTACCCCACTTAATCGCCGTGGCGCGCATCCCCCGTTCGCCAGCTGGCggt                                                                                                 >  5:78949/1‑137 (MQ=255)

                                              cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTTGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                                >  1:61000/1‑138 (MQ=255)

                                              cAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                                <  3:12017/138‑1 (MQ=255)

                                              cAGGCCGACTCTCGAGGATCCCCGGGTACCGAGCTCGAAATCACTGGCCGTCGTTTTACAACGTCGTGACTGGGATAACCCTGGCGATACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTa                                                                                                 <  6:321216/137‑1 (MQ=255)

                                               aGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATa                                                                                              <  5:164121/139‑1 (MQ=255)

                                                   cGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTAAACCCAGGCGGTACCCCACATAATAGCCGTTCCGCACCTACCCCGTTCGCCCGCAGGGGTTATAGCg                                                                                           >  5:291448/1‑138 (MQ=255)

                                                   cGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCg                                                                                                             >  7:239513/1‑120 (MQ=255)

                                                    gACTCTAGAGTCTCCCCGGGTAACGAGCTCGAATTAACTGGCCTTCGTTTTACAACGTCGTGACTGGTAAAACCCTGGCTTTACCAAACTTAATCGCCTTGCAGCACAGCCACATTTCGCCAGCTGGCGTAATAGCTaa                                                                                         <  2:73528/139‑1 (MQ=255)

                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                         <  3:185562/139‑1 (MQ=255)

                                                    gACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                         <  3:250191/139‑1 (MQ=255)

                                                    gACTCTAGAGGATCCCCGGGTACCCAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGaa                                                                                         <  8:220984/139‑1 (MQ=255)

                                                     aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTTCCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCCGCTGGCGTAATAGCg                                                                                           >  2:35079/1‑136 (MQ=255)

                                                     aCTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAag                                                                                        <  3:307577/139‑1 (MQ=255)

                                                              gATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGACGAGGCCCGc                                                                                <  4:68324/138‑1 (MQ=255)

                                                              gATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGTTACCCCACTTAAACGCCATGCAGCACATCCCCCATTCGCCAGCAGGCGTAATATCGAATAGGCCCGCa                                                                               >  4:131438/1‑139 (MQ=255)

                                                               aTCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGcc                                                                                                                                  >  1:79219/1‑87 (MQ=255)

                                                                tCCCCGGGTACCGCGCTCGAATTCACTGGCCGTCGTTCTACAACGTCGTGACTGGGAAACCACTGGCGGTACCCCACTTAATAGCCTTGCAGCACATCCCCATTTCGCCAGCGGGCGTACGAGCGCAGAGGCCCGCAcc                                                                             >  8:344072/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCTGGGTAAACCCTGGCGTTTCCCAACTTAATAGCCGTGCCGCCCATACCCCGTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGcccc                                                                             >  7:88206/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGCCGGGGCAAACCCAGGGGTTACCCAACTTTATCGCCGTGCAGCACATCCCCCATTCGCCCGCGGGCGGAATAGCGAAGCGGCCCGcccc                                                                             >  3:218027/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCTCCTTGCAGCACAACCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGcccccccc                                                                             >  2:51412/1‑134 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACCTCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCAcc                                                                             >  8:61514/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGGCAGCAGTCGTAATAGCGAAGAGGCCCGCaac                                                                             >  4:286161/1‑137 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCATTAGCCCGCAGGCGTAATAGCGAAGAGGCCCCCAcc                                                                             >  6:103748/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCGGGCGTTGCCCCACATAATAGCCCTTCCGCACATCCCCCTTTACCCACCAGGCGTAATAGCGCAGAGGCCCCCAcc                                                                             >  4:345427/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACGGGGAAAACCCTGGCGTTACCCAACTTAATAGCCATGCCGCACAACCCCCTTTCGCCAGCTGGCGGAATAGCGAAGAGGGCCCCAcc                                                                             >  3:363354/1‑139 (MQ=255)

                                                                tCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTACTGACAGTGAAAACCCAGGCGATAACCAAATAAAAAGCCATGCACCACATCCCCCTTTAACAACCAGGCGTAATAGACAAAACGCCCCCAcc                                                                             >  8:137116/1‑139 (MQ=255)

                                                                 ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCg                                                                            >  2:239231/1‑139 (MQ=255)

                                                                 ccccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCAGGCGGTTCCCAACGTAATAGCCGTGCAGCCCATCCCCCTTTCGCCAGCAGGCGGTATAGCGAAGAGGCCCGccccc                                                                            >  6:124897/1‑138 (MQ=255)

                                                                 ccccGGGTACCGAGCTCGAATTCACTGGCCGGCGTTTTAACACGTAGTGACTGTGAAAACCACGGCGGGACCCCACGGTAACGCATTGCAGCCCAGCCCCCAGTCGCCAGCTCGCGAAAAAGCGAAGAAGCCCGCACCg                                                                            >  4:309827/1‑139 (MQ=255)

                                                                   ccGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGTAAAACCAGGCGGTAACCCACTTTATAGCCGTTCAGCCCATCCCCCATTAGCCCCCTGGCGGAATTCGGCAGAGGCCCGCCCCgct                                                                          >  6:205696/1‑137 (MQ=255)

                                                                          ccGAGCTCGACTTCACTGGCCGTCGTTTTACAACGTCGTGACTGTGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                   <  2:187791/139‑1 (MQ=255)

                                                                          ccGAGCTCGAATTCACCGGCCGTCGTTTTACAACGTCGTGACTGGGAAACCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCtt                                                                   <  6:234645/139‑1 (MQ=255)

                                                                           cGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGTCTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCGTGCAGCACATCCCCCTTTAGCCCGCAGGCGTAATAGCGAAGAGGCCCGCCCCCATCGCCCGTc                                                                  >  7:202220/1‑139 (MQ=255)

                                                                           cGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTc                                                                  >  8:298869/1‑139 (MQ=255)

                                                                             aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                <  7:264335/139‑1 (MQ=255)

                                                                             aGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTccc                                                                <  1:304584/139‑1 (MQ=255)

                                                                               cTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTACTCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCaa                                                              <  7:317000/139‑1 (MQ=255)

                                                                                       cACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCCTCCCCCGTTCGCCCGCTGGCGGAATAGCGAAGAGGCCCCCACCCCTCGCCCTTCCCAACACTTgcg                                                      >  4:153622/1‑139 (MQ=255)

                                                                                        aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                     <  1:196624/139‑1 (MQ=255)

                                                                                        aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                     <  3:82564/139‑1 (MQ=255)

                                                                                        aCTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTgcgc                                                     <  4:171274/139‑1 (MQ=255)

                                                                                          tGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCaa                                                              >  6:176760/1‑128 (MQ=255)

                                                                                            gCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGc                                                  <  5:264217/138‑1 (MQ=255)

                                                                                               gtcgtTTTACAACGTCGTGACTGGGAAAACCCTGGCGATACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTga                                              <  5:195952/139‑1 (MQ=255)

                                                                                                 cgtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaat                                            >  6:150513/1‑139 (MQ=255)

                                                                                                  gtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACTTAATAGCCTTGCAGCCCAACCCCCATTCGCCCGCTGGCATAATAGCGAAGAGGCCCGCACCGCTACCCCGTCCCAACAGTTGCGCAGCCGgaatg                                           >  4:369283/1‑139 (MQ=255)

                                                                                                  gtTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCCACATAATCGCCGTGCAGCACCTCCCCCTTTAGCCAGCTGGCGTAAAAGCGAAGAGGCCCCCACCGATCGCCCTTCCCCACAGTTGCGCAGCCGgaatg                                           >  8:114114/1‑139 (MQ=255)

                                                                                                           cgtcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaatggc                                  <  8:165842/139‑1 (MQ=255)

                                                                                                             tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCCCATCCCCCTTTCCCCAGCTGGCGGAATAGCGAATCGGCCCGCACCGATCGCCCTTCCCAACAGTTGAGCCGCCTGAatggcgcatggcgc                                >  4:229200/1‑139 (MQ=255)

                                                                                                             tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATATCGAAGAGGCCCCCACCCATCGCCCGTCCCAACACTTGCGCAGCCGGAATGGCGAAATgcgc                                >  3:14442/1‑139 (MQ=255)

                                                                                                             tcgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGgcgc                                >  6:371491/1‑139 (MQ=255)

                                                                                                              cgtGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCGTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGcc                               >  5:319256/1‑139 (MQ=255)

                                                                                                                      ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                         <  1:250835/137‑1 (MQ=255)

                                                                                                                      ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                         <  8:322549/137‑1 (MQ=255)

                                                                                                                      ggAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGc                         <  3:162072/137‑1 (MQ=255)

                                                                                                                       gAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTgaatggcgaat                                     <  8:201039/124‑1 (MQ=255)

                                                                                                                           aCCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAttt                  <  4:200530/139‑1 (MQ=255)

                                                                                                                            cccTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTAtttt                 <  3:335071/139‑1 (MQ=255)

                                                                                                                              cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct               >  1:337396/1‑139 (MQ=255)

                                                                                                                              cTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGAACGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTtct               <  5:359807/139‑1 (MQ=255)

                                                                                                                                ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTaa                                                                                                >  7:17248/1‑56 (MQ=255)

                                                                                                                                ggCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATACCGAAGAGGCCCGCACCGATCGCCCTTCCCCACAGTTCCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTcc             >  2:95377/1‑139 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGc                                                                                                        <  3:52648/42‑1 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGATTGGCGCCTGATGCGGTATTTTCTCCTTACg        >  5:137837/1‑138 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg        >  7:218416/1‑138 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg        <  6:271002/138‑1 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg        >  5:229593/1‑138 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg        >  1:10/1‑138 (MQ=255)

                                                                                                                                      aCCCAACTTAATCGCCTTGCAGCACATCCCCCTATCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg        <  1:161908/138‑1 (MQ=255)

                                                                                                                                      aCCCAACTTAAGCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACg        <  7:369280/138‑1 (MQ=255)

                                                                                                                                       cccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCG‑CTGATGCGGTATTTTCTCCTTACGCAt     >  2:14173/1‑139 (MQ=255)

                                                                                                                                        ccAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCAt     <  6:295320/139‑1 (MQ=255)

CACAGGAAACAGCTATGACCATGATTACGCCAAGCTTGCATGCCTGCAGGTCGACTCTAGAGGATCCCCGGGTACCGAGCTCGAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGCGCCTGATGCGGTATTTTCTCCTTACGCATCTG  >  Exported/5874‑6151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`