BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	6,277	A→C	5.1%	intergenic (+66/–)	lacZalpha → / –	/vntifkey=4/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	6,277	0	A	C	5.1%	16338.8 / 150.7	5923	intergenic (+66/–)	lacZalpha/–	/vntifkey=4/–
Reads supporting (aligned to +/- strand): ref base A (2648/2860); new base C (396/3); total (3053/2870)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 5987 total aligned reads displayed.

CTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTT  >  Exported/6140‑6415

 ttACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTACCCGCaac                                                                                                                                          >  7:250897/1‑137 (MQ=255)

 ttACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGACTTGTCTGCTCCCGGCATc                                                                                                                                          >  3:150113/1‑139 (MQ=255)

   aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGGCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                          <  2:110173/137‑1 (MQ=255)

   aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                          <  3:350969/137‑1 (MQ=255)

   aCGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                          <  6:125444/137‑1 (MQ=255)

            tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACCCCCGCTGCCGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                               >  1:216048/1‑139 (MQ=255)

            tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                <  1:131019/138‑1 (MQ=255)

            tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                <  1:192371/138‑1 (MQ=255)

            tgCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                               <  2:133712/139‑1 (MQ=255)

                 tATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAg                                                                                                                            >  5:304466/1‑137 (MQ=255)

                   tttCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTATGCGCCCGGCAACCCCTTACAGCCAAGCttg                                                                                                                        >  4:34598/1‑137 (MQ=255)

                      cacaCCGCATATGGTGCAATCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCAACGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      <  4:24675/138‑1 (MQ=255)

                           cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGtct                                                                                                                >  3:229777/1‑139 (MQ=255)

                           cGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGtct                                                                                                                >  1:57069/1‑139 (MQ=255)

                             cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTTCTCCCGGCCTCCGCTTACAGACAAGCGGGGACCCGCTcc                                                                                                              >  1:162881/1‑139 (MQ=255)

                             cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTcc                                                                                                              >  3:71291/1‑139 (MQ=255)

                             cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTa                                                                                                                                    <  2:69875/117‑1 (MQ=255)

                             cATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  7:312436/1‑121 (MQ=255)

                                 tGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                           <  8:49495/138‑1 (MQ=255)

                                 tGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                           <  4:154947/138‑1 (MQ=255)

                                  ggTGCACTCTCATTACCATCTGCTCTGAAGCAGCATAGTGAAGCCAGCCCCGATCCCAGCCAACACCCGCCGACGCGCCCTGACGGGCTAGTCTGCACCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                         <  5:251073/139‑1 (MQ=255)

                                  acacagctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAg                                                                                                         <  3:204872/133‑1 (MQ=255)

                                     gCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTg                                                                                                      >  6:85686/1‑139 (MQ=255)

                                      cACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGAc                                                                                                                               >  5:165492/1‑113 (MQ=255)

                                      cACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAcc                                                                                                                    <  5:65748/124‑1 (MQ=255)

                                       acgcgcAGTACAATCTGCTCTGATGCCGCATAGGTAACCCACCCCCGACACCCGCCCACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                                                    <  1:364179/134‑1 (MQ=255)

                                       aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACCCCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCa                                                                                                    >  7:49217/1‑139 (MQ=255)

                                       aCTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACTCGCACTTACCGGCTATTCTTATCACGGCATCCGCTTACAGACAATCTGTGACCGTCTCCGGGACCTGCa                                                                                                    >  4:216531/1‑139 (MQ=255)

                                        ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCATCCGGGCTTTTCTTCTCCCGGCAAGCGCTTACCGCCAAGCTGTGACCGTAGCCCGGGGCGGCAt                                                                                                   >  7:147583/1‑139 (MQ=255)

                                        ctctCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCAt                                                                                                   >  5:350899/1‑139 (MQ=255)

                                         tctcAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCg                                                                                                                   >  1:294213/1‑122 (MQ=255)

                                            cAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCCTCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCGGCCTgtgt                                                                                               >  8:214464/1‑139 (MQ=255)

                                             aGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGACGGGCTTGTCTGCGCCCCGCATCCGCATACAGACAAACTGGGACCGGCTCCCGGAGCGGCCtggtt                                                                                               >  4:64502/1‑135 (MQ=255)

                                              gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCt                                                                                                                          >  8:100593/1‑110 (MQ=255)

                                              gTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCa                                                                                             <  7:202553/139‑1 (MQ=255)

                                              gTACAATCTGCTCTGAGGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCa                                                                                             <  3:176193/139‑1 (MQ=255)

                                                aCAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGCCCGTCTCCGGGAGCTGCATGTGTCa                                                                                             >  3:45742/1‑137 (MQ=255)

                                                   aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGCCACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGt                                                                                        >  4:318148/1‑139 (MQ=255)

                                                   aTCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCAGGGCGTGTATGCTCCCCGCCTCCGCATACAGACAAGATGTGACCGTCTCCCGGGGCGTCAAGTGTCAGCGGt                                                                                        >  4:368092/1‑139 (MQ=255)

                                                    tCTGCTCTGATGCCGCATATTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCATACGGGCTTGTCTGATCCCGGCCGCCGCTTAAACAACAGCTGTGACCCTCTCCGGGCGCGGCAAGTGTGAGCggct                                                                                       >  4:294757/1‑137 (MQ=255)

                                                    tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                                       >  3:120159/1‑139 (MQ=255)

                                                    tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                                       >  8:267506/1‑139 (MQ=255)

                                                    tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTACCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtt                                                                                       >  5:274107/1‑139 (MQ=255)

                                                    tCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCATGTCTGCTACCCGCATCCGCTTAAACACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTGagagtgt                                                                                       >  8:334253/1‑136 (MQ=255)

                                                     cTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACa                                                                                                                              <  2:52196/99‑1 (MQ=255)

                                                       gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTTCTCCCCGCATACGCTTACAGACAAGCAGTGACCCTCTCCGGGAGCTGCATGTGTCAGAggctgtg                                                                                    >  4:126596/1‑134 (MQ=255)

                                                       gCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGtttt                                                                                     >  2:249048/1‑138 (MQ=255)

                                                        ctctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTTCTCCCCGCAGCCCCTTAAACACCATCTGTGCCCGGCTCCGGGCGCTGCAGGTGTCAGAGGTTTTcc                                                                                   >  3:30082/1‑138 (MQ=255)

                                                         tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGCCCGGCGTGTCTGCTCCCGGCATACGCTTACAGACAAGCAGTGACCCTCTCCCGGCGCTGCCTTTGTCAGAGGTTTTccc                                                                                  >  2:98096/1‑137 (MQ=255)

                                                         tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACCAGCTGTGACCGTCTCCGGGCGCTGCATGTGTCAGAGGTTTTCAc                                                                                  >  8:310596/1‑139 (MQ=255)

                                                         tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCggg                                                                                                           <  5:145292/114‑1 (MQ=255)

                                                         tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCGGCATGTGTCAGAGGTTTTCAc                                                                                  >  1:323519/1‑139 (MQ=255)

                                                         tctGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCGGCCGGGCTTGTCTGCTCCCCGCATCCGCTTACAGCCCAGCTGTGACCGTCGCCCGGGGCTGCATGTGTCAGAGGGTTTCAc                                                                                  >  3:258916/1‑139 (MQ=255)

                                                             aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGAc                                                                                                                     <  8:242706/100‑1 (MQ=255)

                                                             aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGtc                                                                              <  3:326676/139‑1 (MQ=255)

                                                             aTGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCCTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGt                                                                               >  7:121755/1‑138 (MQ=255)

                                                              tGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCGTGTCTGCTCCCGGCATCCGCGTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGtca                                                                             >  2:225046/1‑139 (MQ=255)

                                                                  gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      <  1:26670/94‑1 (MQ=255)

                                                                  gCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCAc                                                                         >  3:281525/1‑139 (MQ=255)

                                                                       gTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATc                                                                                                                                          <  7:226007/69‑1 (MQ=255)

                                                                           agccagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAAcgcg                                                                <  5:327847/139‑1 (MQ=255)

                                                                              cagccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCga                                                             <  5:223122/139‑1 (MQ=255)

                                                                                gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  6:63065/1‑70 (MQ=255)

                                                                                gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGa                                                                                                                      <  8:361503/80‑1 (MQ=255)

                                                                                gccCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCgaga                                                           >  7:35811/1‑139 (MQ=255)

                                                                                 ccccGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCCGCATCCGCTTACAGACAAGCTGAGACCCTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCAACGTCATCACCGAAACGCGCGAGAc                                                          >  7:332716/1‑139 (MQ=255)

                                                                                     gccacCCGCCAACCCCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCTGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGaaa                                                      <  2:349795/137‑1 (MQ=255)

                                                                                      acacCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTATGCTCCCGGCATACGCTTACAGACAAGCTGTGACCGTCGCCGGGAGCTGCATGTGTCAGAGGGTTTCACCGTCATCACCCAAACGCGCGCGACGAAAg                                                     >  4:91397/1‑139 (MQ=255)

                                                                                          ccGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGcc                                                 <  7:176176/139‑1 (MQ=255)

                                                                                           cGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCt                                                <  7:292259/139‑1 (MQ=255)

                                                                                           cGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCt                                                <  4:269663/139‑1 (MQ=255)

                                                                                            gCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCCAACGCTTTCAGACCAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGGTTTCACCGTCATCACCGAAACGCGCGAGGCGAAAGGGCCTc                                               >  1:237566/1‑139 (MQ=255)

                                                                                                acacCCGCTGACGCGCCCTGACGGGCTTGTCTGCGCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGa                                           <  6:290874/139‑1 (MQ=255)

                                                                                                      gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  8:289124/1‑48 (MQ=255)

                                                                                                      gCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGcc                                     <  6:314167/139‑1 (MQ=255)

                                                                                                       cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCt                                    <  5:194909/139‑1 (MQ=255)

                                                                                                       cTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGGCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCt                                    <  6:151561/139‑1 (MQ=255)

                                                                                                          aCGCGCCCGGCCGGGGTTGTCTGCTCCCGGAATCCGCTTACAGCCAAGCTGGGACCGTCTCGGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGACACGCGCGAGACGAAAGGGCCTCGTGATACGCCTa                                   <  6:236552/137‑1 (MQ=255)

                                                                                                           cgcgCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGa                                                                                                                                >  2:208063/1‑43 (MQ=255)

                                                                                                           cgcgCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCt                                    <  6:200673/135‑1 (MQ=255)

                                                                                                             cgcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTAttttt                              <  5:11632/139‑1 (MQ=255)

                                                                                                              gcCCTGTCGGGCTTGTCTGCTCGCGGCATCCGCTGACAGACAAGCTGTGAGCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  6:264809/139‑1 (MQ=255)

                                                                                                              gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  6:342405/139‑1 (MQ=255)

                                                                                                              gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  6:36428/139‑1 (MQ=255)

                                                                                                              gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  8:159694/139‑1 (MQ=255)

                                                                                                              gcCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCGGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             <  7:76244/139‑1 (MQ=255)

                                                                                                              gcCCTGACGGGCTGGTCTGCTCCCGGCATCCGCTTACAGCCAAGCTGTGACCGGCTCCGGGAGCGGCATGGGCCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAACGGGCCTCGTGATACGCCTAttttaa                             <  8:189142/139‑3 (MQ=255)

                                                                                                                       ggCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTTTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAAt                    <  2:279808/139‑1 (MQ=255)

                                                                                                                       ggCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAAt                    <  5:373360/139‑1 (MQ=255)

                                                                                                                       ggCTTGGCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAAt                    <  2:168520/139‑1 (MQ=255)

                                                                                                                         cTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  <  1:7712/139‑1 (MQ=255)

                                                                                                                          ttGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGt                  >  8:76095/1‑138 (MQ=255)

                                                                                                                            gTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTc                 <  8:13460/137‑1 (MQ=255)

                                                                                                                             tCTGCTCCCTGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  6:174783/138‑1 (MQ=255)

                                                                                                                             tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  3:95658/138‑1 (MQ=255)

                                                                                                                             tCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  7:269900/138‑1 (MQ=255)

                                                                                                                             tCTGCTCCCGGCATCAGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCAt               <  2:315616/138‑1 (MQ=255)

                                                                                                                                  tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTta                             >  1:100373/1‑119 (MQ=255)

                                                                                                                                  tCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGataa          >  4:18614/1‑138 (MQ=255)

                                                                                                                                         aTCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGtt  <  5:97108/139‑1 (MQ=255)

CTTACGCATCTGTGCGGTATTTCACACCGCATATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGCCCCGACACCCGCCAACACCCGCTGACGCGCCCTGACGGGCTTGTCTGCTCCCGGCATCCGCTTACAGACAAGCTGTGACCGTCTCCGGGAGCTGCATGTGTCAGAGGTTTTCACCGTCATCACCGAAACGCGCGAGACGAAAGGGCCTCGTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTT  >  Exported/6140‑6415

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`