Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 1,428,900 A→G 5.6% K44E (AAA→GAA)  U712_07335 → putative oxidoreductase ykwC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068811,428,9000AG 5.6% 226.0 / 3.1 71K44E (AAA→GAA) U712_07335putative oxidoreductase ykwC
Reads supporting (aligned to +/- strand):  ref base A (43/24);  new base G (2/2);  total (45/26)
Fisher's exact test for biased strand distribution p-value = 6.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGACCTTGAAAAAAACAATTGGTTTTATCGGTCTTGGAGTAATGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAAAGAAGGTG  >  CP006881/1428764‑1429038
                                                                                                                                        |                                                                                                                                          
aaTGACCTTGAAAAAAACAATTGGTTTTATCGGTCTTGGAGTAATGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCaaa                                                                                                                                            >  4:215372/1‑137 (MQ=255)
                   aTTGGTTTTATCGGTCTTGGAGTAATGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATa                                                                                                                       <  3:215372/139‑1 (MQ=255)
                            aTCGGTCTTGGAGTAATGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCa                                                                                                                 >  7:63051/1‑136 (MQ=255)
                                  cTTGGAGTAATGGGAAATAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATAGGTGTCCACCAGTACAAAAGAAAAAGCAGAACGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGCTCttt                                                                                                        >  2:45618/1‑139 (MQ=255)
                                           aTGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGgcgc                                                                                                                                     <  7:287134/101‑1 (MQ=255)
                                           aTGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGgcgc                                                                                                                                     >  8:287134/1‑101 (MQ=255)
                                           aTGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAaagaag                                                                                               >  2:200789/1‑139 (MQ=255)
                                           aTGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAaagaag                                                                                               >  2:132850/1‑139 (MQ=255)
                                           aTGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAaagaag                                                                                               <  1:106635/139‑1 (MQ=255)
                                           aTGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAaagaag                                                                                               >  1:147303/1‑139 (MQ=255)
                                              tgAAAAAGCATGGCATCACATATTCTCCATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCgg                                                                                            <  5:215778/138‑1 (MQ=255)
                                              ggAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCgg                                                                                            <  1:96263/139‑1 (MQ=255)
                                                   aaGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGAt                                                                                                                        <  5:207045/106‑1 (MQ=255)
                                                   aaGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGAAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGAt                                                                                                                        >  6:207045/1‑106 (MQ=255)
                                                          gCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTAtcatca                                                                                <  8:45713/139‑1 (MQ=255)
                                                               acaTATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATg                                                                           >  2:144603/1‑139 (MQ=255)
                                                                caTATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAACAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATgg                                                                          >  5:59681/1‑139 (MQ=255)
                                                                    ttCTCAATGACGGCCATCCTGTATTGGCGGACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCgg                                                                      <  8:269669/139‑1 (MQ=255)
                                                                     tctcAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTatcat                                                                                  <  1:26694/126‑1 (MQ=255)
                                                                     tctcAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTatcat                                                                                  >  2:26694/1‑126 (MQ=255)
                                                                      ctcAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAACAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGc                                                                     <  6:59681/138‑1 (MQ=255)
                                                                         aaTGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAGAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCgg                                                                                            <  4:74452/112‑1 (MQ=255)
                                                                         aaTGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAGAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCgg                                                                                            <  4:79633/112‑1 (MQ=255)
                                                                         aaTGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAGAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCgg                                                                                            >  3:74452/1‑112 (MQ=255)
                                                                         aaTGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAGAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCgg                                                                                            >  3:79633/1‑112 (MQ=255)
                                                                         aaTGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATc                                                                 >  1:46210/1‑139 (MQ=255)
                                                                         aaTGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATc                                                                 >  3:86767/1‑139 (MQ=255)
                                                                              cGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGt                                                            <  1:132850/139‑1 (MQ=255)
                                                                                 ccATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTat                                                                                     >  2:313174/1‑111 (MQ=255)
                                                                                 ccATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTat                                                                                     <  1:313174/111‑1 (MQ=255)
                                                                                              gtgtACACCCTGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGATAGATACGGTCAAAGATCTTTCGATAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAgtg                                             >  4:211503/1‑138 (MQ=255)
                                                                                              gtgtACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAgtg                                             >  5:274310/1‑138 (MQ=255)
                                                                                              gtgtACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAgtg                                             >  1:83813/1‑138 (MQ=255)
                                                                                              gtgtACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCAGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAgtg                                             >  4:21263/1‑138 (MQ=255)
                                                                                                gtACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATCTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTAt                                          >  1:359970/1‑139 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATgg                                                                          <  8:73057/106‑1 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATgg                                                                          >  7:73057/1‑106 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTAtt                                         >  3:321414/1‑139 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTAtt                                         >  5:238028/1‑139 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTAtt                                         >  5:31831/1‑139 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTAtt                                         >  7:18422/1‑139 (MQ=255)
                                                                                                 tACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTAtt                                         >  5:83191/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATa                                                                                                                       <  3:350823/59‑1 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATa                                                                                                                       >  4:350823/1‑59 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAaagaag                                                                                               >  8:31534/1‑83 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAaagaag                                                                                               <  7:31534/83‑1 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAg                                                             <  4:369414/117‑1 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAg                                                             >  3:369414/1‑117 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGa                                                          <  8:194476/120‑1 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGa                                                          >  7:194476/1‑120 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTc                                       >  6:325762/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTc                                       >  8:52864/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTc                                       >  2:260419/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTc                                       >  2:310093/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTc                                       >  6:244524/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGAGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTc                                       >  1:233779/1‑139 (MQ=255)
                                                                                                   cacCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGAGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGAGTATTTc                                       >  7:362335/1‑139 (MQ=255)
                                                                                                      ccGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGa                                    <  1:144603/139‑1 (MQ=255)
                                                                                                        ggACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTgaa                                                   <  2:90749/122‑1 (MQ=255)
                                                                                                        ggACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTgaa                                                   >  1:90749/1‑122 (MQ=255)
                                                                                                        ggACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAg                                  >  7:122924/1‑139 (MQ=255)
                                                                                                        ggACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAg                                  >  2:116827/1‑139 (MQ=255)
                                                                                                              aaGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATg                             >  5:114088/1‑138 (MQ=255)
                                                                                                                gAAAAAGCAGAAATCATACTTCAAAAATGCGCGATCTGGAAAGATACGGTCAAATATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGaa                          <  5:319961/139‑1 (MQ=255)
                                                                                                                          aaaGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGaaaa                >  6:39145/1‑139 (MQ=255)
                                                                                                                                tACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCaa           >  2:163359/1‑138 (MQ=255)
                                                                                                                                 aCTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCaa                               <  2:67073/117‑1 (MQ=255)
                                                                                                                                 aCTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCaa                               >  1:67073/1‑117 (MQ=255)
                                                                                                                                 aCTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCaa           >  3:172941/1‑137 (MQ=255)
                                                                                                                                   ttCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAaaga       <  6:83191/139‑1 (MQ=255)
                                                                                                                                     cAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAaaga       <  2:147303/137‑1 (MQ=255)
                                                                                                                                     cAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAaaga       <  6:274310/137‑1 (MQ=255)
                                                                                                                                        aaaGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAAAGAAGGTg  >  6:116416/1‑139 (MQ=255)
                                                                                                                                        aaaGGCGCGATCTGGAAAGATACGGGCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAAAGAAGGTg  <  5:157183/139‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                          
AATGACCTTGAAAAAAACAATTGGTTTTATCGGTCTTGGAGTAATGGGAAAAAGCATGGCATCACATATTCTCAATGACGGCCATCCTGTATTGGTGTACACCCGGACAAAAGAAAAAGCAGAAAGCATACTTCAAAAAGGCGCGATCTGGAAAGATACGGTCAAAGATCTTTCGAAAGAAGCGGATGTTATCATCACAATGGTCGGCTATCCCAGTGATGTTGAAGAAGTGTATTTCGGAAGCAATGGAATTATTGAAAACGCAAAAGAAGGTG  >  CP006881/1428764‑1429038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑