Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,189,447 T→C 7.2% T243A (ACT→GCT)  U712_10970 ← Tryptophan synthase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,189,4470TC 7.2% 163.8 / 4.3 59T243A (ACT→GCT) U712_10970Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base T (28/25);  new base C (2/2);  total (30/29)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.95e-01

TGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACA  >  CP006881/2189318‑2189580
                                                                                                                                 |                                                                                                                                     
tGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTCAtgatgac                                                                                                                              >  2:184085/1‑138 (MQ=255)
  aTAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGcc                                                                                                                            <  6:314958/139‑1 (MQ=255)
            aGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAg                                                                                                                  >  1:172316/1‑139 (MQ=255)
                                     gCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCa                                                                                         <  5:339094/139‑1 (MQ=255)
                                                 aCCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCCGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACg                                                                             >  8:92030/1‑139 (MQ=255)
                                                 aCCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACg                                                                             >  7:70751/1‑139 (MQ=255)
                                                   cTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGac                                                                           >  8:220511/1‑139 (MQ=255)
                                                   cTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGac                                                                           >  7:254981/1‑139 (MQ=255)
                                                                ataCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCgg                                                                                                                          <  1:328258/79‑1 (MQ=255)
                                                                ataCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCgg                                                                                                                          >  2:328258/1‑79 (MQ=255)
                                                                ataCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAAtt                                                              >  6:10562/1‑139 (MQ=255)
                                                                ataCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAAtt                                                              >  3:250189/1‑139 (MQ=255)
                                                                ataCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAAt                                                               >  4:49801/1‑138 (MQ=255)
                                                                 taCAAGCCGCCAAACGCCCTTGCGTAATCTTCAGACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAAttt                                                             >  1:118632/1‑139 (MQ=255)
                                                                 taCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAAttt                                                             >  7:59692/1‑139 (MQ=255)
                                                                 taCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAAttt                                                             >  1:49304/1‑139 (MQ=255)
                                                                 taCAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATATCTCGTTTCAGTGCTGATGCGCCGGTCCTGTAGCTCTTCTATTTTTCTCAGGAGCGCACTACCCACTACGAAACCGTCCCAAAtt                                                              >  1:140969/1‑138 (MQ=255)
                                                                      gccgccAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATttcatt                                                         >  6:176467/1‑138 (MQ=255)
                                                                       ccgccACACGCACTTGCGTAATCTTCAAAGTCCTGCAGCGCGTGATCGCTCGGTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATttcattca                                                       <  6:349810/139‑1 (MQ=255)
                                                                       ccgccAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATttcattca                                                       <  5:274881/139‑1 (MQ=255)
                                                                       ccgccAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATttcattca                                                       <  2:172316/139‑1 (MQ=255)
                                                                       ccgccAAACGCCCTTGCGTAAGCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATttcattca                                                       <  6:9166/139‑1 (MQ=255)
                                                                         gccAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATc                                                     >  3:93983/1‑139 (MQ=255)
                                                                         gccAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATc                                                     >  7:222339/1‑139 (MQ=255)
                                                                         gccAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTAATTCATc                                                     >  6:30402/1‑139 (MQ=255)
                                                                           ctaaCGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCtt                                                   >  4:197611/3‑139 (MQ=255)
                                                                             aaCGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCa                                                 <  4:250189/139‑1 (MQ=255)
                                                                                gCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTGTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGa                                              <  7:45025/139‑1 (MQ=255)
                                                                                        gTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcg                                      <  8:222339/139‑1 (MQ=255)
                                                                                         tAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcgc                                     >  3:270048/1‑139 (MQ=255)
                                                                                           aTCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcgcg                                    <  7:368311/138‑1 (MQ=255)
                                                                                           aTCTTCAAACTCCTGCAGCGCTTGATCTCTCGGTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcgcg                                    <  1:184085/138‑1 (MQ=255)
                                                                                            tcctCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAACCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                  >  3:372857/4‑139 (MQ=255)
                                                                                            tCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAgcg                                                                                           <  3:115417/82‑1 (MQ=255)
                                                                                            tCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAgcg                                                                                           >  4:115417/1‑82 (MQ=255)
                                                                                            tCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGt                                  >  3:194382/1‑139 (MQ=255)
                                                                                            tCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGt                                  >  6:120646/1‑139 (MQ=255)
                                                                                                        tGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCg                       >  5:316912/1‑138 (MQ=255)
                                                                                                        tGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCg                       >  5:298947/1‑138 (MQ=255)
                                                                                                        tGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCg                       >  4:203311/1‑138 (MQ=255)
                                                                                                          cAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTAGCGGTTTGATATACCGAAc                    >  3:227534/1‑139 (MQ=255)
                                                                                                                ttGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa              <  4:194382/139‑1 (MQ=255)
                                                                                                                  gATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa              <  8:70751/137‑1 (MQ=255)
                                                                                                                  gATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa              <  3:203311/137‑1 (MQ=255)
                                                                                                                  gATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa              <  4:270048/137‑1 (MQ=255)
                                                                                                                       ctcGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCg       >  1:73684/1‑139 (MQ=255)
                                                                                                                        tcGTTTCCGTGCGGATGAGCCGGTCCTTTCGCTCTTCTTTTTTTCTGCCGAGCGCACTACCCACTACGACACCGACACAAATTTCATTCATCTTCATCCCCTGTCCGCGGTTTGATATACCGAACCCGACAGCAAc         <  2:241885/136‑1 (MQ=255)
                                                                                                                        tcGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAAc         <  6:298947/136‑1 (MQ=255)
                                                                                                                        tcGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAAc         <  6:316912/136‑1 (MQ=255)
                                                                                                                          gTTTCCGGGCGGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa    <  2:49304/139‑1 (MQ=255)
                                                                                                                          gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa    <  3:197611/139‑1 (MQ=255)
                                                                                                                          gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa    <  2:73684/139‑1 (MQ=255)
                                                                                                                          gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa    <  7:220511/139‑1 (MQ=255)
                                                                                                                          gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa    <  5:10562/139‑1 (MQ=255)
                                                                                                                          gTTTCAGCGCTGATGAACCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa    <  4:372857/139‑1 (MQ=255)
                                                                                                                          gTTCCAGTGCTGATGAGCCGGGCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTCCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa    <  6:308629/139‑1 (MQ=255)
                                                                                                                             tCAGAGCTGCTGACCCGGCCCTTTAGCTCTTCTATTTTTCTGCCGCGCGCACTTCCCACTACGACACCGTCACAACTTTCATTCCTCTTCATGACCTGTTCGCGGTTTCATATACCGAACCCGACAGCAACCGGAACa  <  5:26957/138‑1 (MQ=255)
                                                                                                                              cAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa    >  8:127839/1‑135 (MQ=255)
                                                                                                                              cAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa    <  7:127839/135‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                     
TGATAAGGCTTCAGTTGTTTTAAATGTTCTTTGATACGCAAATCCAGTCACCTCATTTTAAACTATACAAGCCGCCAAACGCCCTTGCGTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACA  >  CP006881/2189318‑2189580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑