Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	257,096	G→T	6.2%	T161K (ACG→AAG)	U712_01200 ←	putative glucosamine‑6‑phosphate deaminase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	257,096	0	G	T	6.2%	205.7 / 4.2	65	T161K (ACG→AAG)	U712_01200	putative glucosamine‑6‑phosphate deaminase 2
Reads supporting (aligned to +/- strand):  ref base G (31/30);  new base T (2/2);  total (33/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.63e-01

TCTGCTTTTTCCTCGCCGCTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATGAGATCCTCGT  >  CP006881/256961‑257231                                                                                                                                        |                                                                                                                                        tCTGCTTTTTCCTCGCCGCTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGc                                                                                                                                      <  1:69911/139‑1 (MQ=255) tCTGCTTTTTCCTCGCCGCTTGCTAAAAGCACAATATGCTTGCTGAATACCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGc                                                                                                                                      <  5:259369/139‑1 (MQ=255)           ccTCGCCGCTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGagag                                                                                                                            >  3:304344/1‑139 (MQ=255)               gccgcTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCtt                                                                                                                         >  3:212507/1‑138 (MQ=255)               gccgcTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCtt                                                                                                                         >  2:330631/1‑138 (MQ=255)               gccgcTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCtt                                                                                                                         >  1:76859/1‑138 (MQ=255)                ccgcTAGCTAAAAGCACAATATGTTTGCTGATTTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTca                                                                                                                       <  1:373119/139‑1 (MQ=255)                      gctcacaGCACAATCTGTTGGCTGACATCCCTGATGGTTTTACTGCCCATTGCACGCGCGAGTCTCGGAACCCGCACCGGATCGCCGCCGAAGAATCTGGCATGTGCCTGTCTCGTGCTTGCCGAGAGCTTCacaaca                                                                                                                  <  7:339558/132‑1 (MQ=255)                           aaGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTc                                                                                                            <  4:212507/139‑1 (MQ=255)                                      gTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGCACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCaa                                                                                                  >  8:55475/1‑138 (MQ=255)                                      gTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCaa                                                                                                  >  2:245931/1‑138 (MQ=255)                                        ttGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGACCCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGTTCTTCAAAAt                                                                                               >  2:170295/1‑139 (MQ=255)                                        ttGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAAt                                                                                               >  3:224067/1‑139 (MQ=255)                                           cTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAAt                                                                                               >  6:348508/1‑136 (MQ=255)                                           cTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAAt                                                                                               >  4:163917/1‑136 (MQ=255)                                           cTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTGTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAAt                                                                                               >  8:211282/1‑136 (MQ=255)                                               aTTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATcc                                                                                        >  6:340020/1‑139 (MQ=255)                                                ttCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATccc                                                                                       >  8:4738/1‑139 (MQ=255)                                                     tgatgGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGCAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGtt                                                                                   >  2:98470/1‑138 (MQ=255)                                                     tgatgGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCCCAACACGTGTCCGGTCTTCAAAATCAGATCCCGGtt                                                                                   >  3:8416/1‑138 (MQ=255)                                                     tgatgGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGtt                                                                                   >  4:246271/1‑138 (MQ=255)                                                          gTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATTGCCGCCGAAGAATCTGGCATTTGCCTGTATCTTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTa                                                                              >  8:167198/1‑138 (MQ=255)                                                          gTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATTGCCGCCGAAGAATCTGGCATTTGCCTGTATCTTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTa                                                                              >  8:172118/1‑138 (MQ=255)                                                          gTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTaa                                                                             <  5:316106/139‑1 (MQ=255)                                                             ttAATGCCCATTGAAATCGCGAGTCTCGGACCCAGCACCGGGTCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAAc                                                                          >  8:34410/1‑139 (MQ=255)                                                             ttAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTaaaa                                                                           <  8:62585/138‑1 (MQ=255)                                                             ttAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAAc                                                                          >  3:199374/1‑139 (MQ=255)                                                              tAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAAc                                                                          <  7:99107/138‑1 (MQ=255)                                                                   cccATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGata                                                                     <  3:80707/138‑1 (MQ=255)                                                                   cccATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCATGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGatat                                                                    >  7:11047/1‑139 (MQ=255)                                                                    ccATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCCCAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATg                                                                   >  6:77520/1‑139 (MQ=255)                                                                     cATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGAt                                                                                          <  7:256489/115‑1 (MQ=255)                                                                     cATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGAt                                                                                          >  8:256489/1‑115 (MQ=255)                                                                     cATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATgg                                                                  >  5:101085/1‑139 (MQ=255)                                                                                cgAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAACACCGATATGGCCATTGGCGcc                                                       >  1:124571/1‑139 (MQ=255)                                                                                cgAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGcc                                                       >  2:164270/1‑139 (MQ=255)                                                                                cgAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGcc                                                       >  1:319955/1‑139 (MQ=255)                                                                                               gCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTg                                        >  2:27043/1‑139 (MQ=255)                                                                                               gCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTg                                        >  6:153143/1‑139 (MQ=255)                                                                                                       tCGCCGCCGAAGAATCTGGCAATTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAACCCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCaa                                <  1:127284/139‑1 (MQ=255)                                                                                                        cgccgcCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAAt                               >  6:364159/1‑139 (MQ=255)                                                                                                            gccgAAGAATCTGGCATTTGCCTGTATCTTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCt                           <  7:167198/139‑1 (MQ=255)                                                                                                            gccgAAGAATCTGGCATTTGCCTGTATCTTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCt                           <  7:172118/139‑1 (MQ=255)                                                                                                             ccgAAGAATCTGGAATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCAGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGTCATCAATGCCTc                          >  6:209552/1‑139 (MQ=255)                                                                                                                     tCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTgg                  >  2:331510/1‑139 (MQ=255)                                                                                                                        ggCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTg                   >  2:179711/1‑135 (MQ=255)                                                                                                                         gCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTgg                  <  5:340020/135‑1 (MQ=255)                                                                                                                         gCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTgg                  <  1:179711/135‑1 (MQ=255)                                                                                                                            tttGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATga           <  1:330631/139‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  5:153143/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  1:164270/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  1:245931/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  1:27043/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  1:53398/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  2:108032/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  7:211282/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  3:163917/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  4:343116/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGGCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  4:224067/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCTTTCCGAGAGCGTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  4:8416/138‑1 (MQ=255)                                                                                                                               gCCTGTATCGTGCGTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATgaga         <  7:55475/138‑1 (MQ=255)                                                                                                                               gCCTGTATAGTGCTTTCCGAGAGCTTCACTACCGGTGTCCGGTCTTCAAACTCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGTCGGATgaga         <  2:45444/138‑1 (MQ=255)                                                                                                                                ccTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATGAGATc       <  7:4738/139‑1 (MQ=255)                                                                                                                                ccTGTATCGTGCCTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGCTCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATGAGATc       <  7:135894/139‑1 (MQ=255)                                                                                                                                      tCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATGAGATCCTCGt  <  2:76859/138‑1 (MQ=255)                                                                                                                                        |                                                                                                                                        TCTGCTTTTTCCTCGCCGCTTGCTAAAAGCACAATATGTTTGCTGAATTCCATGATGGTTTTAATGCCCATTGAAATCGCGAGTCTCGGAACCAGCACCGGATCGCCGCCGAAGAATCTGGCATTTGCCTGTATCGTGCTTTCCGAGAGCTTCACAACACGTGTCCGGTCTTCAAAATCAGATCCCGGTTCATTAAAACCGATATGGCCATTGGCGCCGATGCCGAGAATTTGGACATCAATGCCTCCCGCCTGGCGGATGAGATCCTCGT  >  CP006881/256961‑257231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑