Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,189,544 G→A 8.2% R210R (CGC→CGT)  U712_10970 ← Tryptophan synthase alpha chain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,189,5440GA 8.2% 224.3 / 6.4 73R210R (CGC→CGT) U712_10970Tryptophan synthase alpha chain
Reads supporting (aligned to +/- strand):  ref base G (26/41);  new base A (3/3);  total (29/44)
Fisher's exact test for biased strand distribution p-value = 6.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAG  >  CP006881/2189406‑2189679
                                                                                                                                          |                                                                                                                                       
gTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcg                                                                                                                                         <  8:222339/139‑1 (MQ=255)
 tAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcgc                                                                                                                                        >  3:270048/1‑139 (MQ=255)
   aTCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcgcg                                                                                                                                       <  7:368311/138‑1 (MQ=255)
   aTCTTCAAACTCCTGCAGCGCTTGATCTCTCGGTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTcgcg                                                                                                                                       <  1:184085/138‑1 (MQ=255)
    tcctCAAACTCCTGCAGCGCCTGATTTCTCGTTTCAGCGCTGATGAACCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGt                                                                                                                                     >  3:372857/4‑139 (MQ=255)
    tCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGt                                                                                                                                     >  6:120646/1‑139 (MQ=255)
    tCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGt                                                                                                                                     >  3:194382/1‑139 (MQ=255)
                tGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCg                                                                                                                          >  5:316912/1‑138 (MQ=255)
                tGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCg                                                                                                                          >  5:298947/1‑138 (MQ=255)
                tGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCg                                                                                                                          >  4:203311/1‑138 (MQ=255)
                  cAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTAGCGGTTTGATATACCGAAc                                                                                                                       >  3:227534/1‑139 (MQ=255)
                        ttGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa                                                                                                                 <  4:194382/139‑1 (MQ=255)
                          gATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa                                                                                                                 <  8:70751/137‑1 (MQ=255)
                          gATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa                                                                                                                 <  4:270048/137‑1 (MQ=255)
                          gATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa                                                                                                                 <  3:203311/137‑1 (MQ=255)
                               ctcGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCg                                                                                                          >  1:73684/1‑139 (MQ=255)
                                tcGTTTCCGTGCGGATGAGCCGGTCCTTTCGCTCTTCTTTTTTTCTGCCGAGCGCACTACCCACTACGACACCGACACAAATTTCATTCATCTTCATCCCCTGTCCGCGGTTTGATATACCGAACCCGACAGCAAc                                                                                                            <  2:241885/136‑1 (MQ=255)
                                tcGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAAc                                                                                                            <  6:316912/136‑1 (MQ=255)
                                tcGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAAc                                                                                                            <  6:298947/136‑1 (MQ=255)
                                  gTTTCCGGGCGGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  2:49304/139‑1 (MQ=255)
                                  gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  7:220511/139‑1 (MQ=255)
                                  gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  2:73684/139‑1 (MQ=255)
                                  gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  5:10562/139‑1 (MQ=255)
                                  gTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  3:197611/139‑1 (MQ=255)
                                  gTTTCAGCGCTGATGAACCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa                                                                                                       <  4:372857/139‑1 (MQ=255)
                                  gTTCCAGTGCTGATGAGCCGGGCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTCCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  6:308629/139‑1 (MQ=255)
                                     tCAGAGCTGCTGACCCGGCCCTTTAGCTCTTCTATTTTTCTGCCGCGCGCACTTCCCACTACGACACCGTCACAACTTTCATTCCTCTTCATGACCTGTTCGCGGTTTCATATACCGAACCCGACAGCAACCGGAACa                                                                                                     <  5:26957/138‑1 (MQ=255)
                                      cAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa                                                                                                       <  7:127839/135‑1 (MQ=255)
                                      cAGCGCTGATGAGCCGGTCCTTTAATTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACTAATTTCATTCATCTTTATGACCTGTTCACGGTTTGATATACCGAACCCTACAGCAACCGGaa                                                                                                       >  8:127839/1‑135 (MQ=255)
                                          gCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGCGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTg                                                                                               <  3:9826/139‑1 (MQ=255)
                                          gCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCt                                                                                                >  4:188057/1‑138 (MQ=255)
                                          gCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTg                                                                                               >  4:9826/1‑139 (MQ=255)
                                           cTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACTACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTg                                                                                               <  5:30402/138‑1 (MQ=255)
                                           cTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTg                                                                                               <  3:188057/138‑1 (MQ=255)
                                            tgatgaGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATATTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTgag                                                                                             <  8:254981/139‑1 (MQ=255)
                                               tgaGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATc                                                                                          <  4:93983/139‑1 (MQ=255)
                                                   ccGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGCACCCGACAGCAACCGGAACAGTGCTGAGATCCTTc                                                                                      >  1:280060/1‑139 (MQ=255)
                                                   ccGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCtt                                                                                       >  1:57806/1‑138 (MQ=255)
                                                    cGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCa                                                                                     <  2:118632/139‑1 (MQ=255)
                                                    cGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCa                                                                                     <  8:306998/139‑1 (MQ=255)
                                                       tCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATAACGAACCCGACAGCAAACGGAACAGTGCTGAGATCCTTCACTg                                                                                  >  2:192085/1‑139 (MQ=255)
                                                              gCTCTTATCTTTTTCTGAAGCGCTCACTTCACACTACGCCACCGTCAAAAATTTCATTCATCTTCCTGACCGGTTCGCGTGTTTATCTACCTAACCCGACAGCACCCGGAACAGTGCTGAGATCCTTCAATGTCCGGa                                                                            <  5:158928/138‑1 (MQ=255)
                                                                 cttctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGaa                                                                        >  6:272986/1‑139 (MQ=255)
                                                                  ttctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGa                                                                         <  5:176467/137‑1 (MQ=255)
                                                                   tctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACg                                                                      >  3:74817/1‑139 (MQ=255)
                                                                   tctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACg                                                                      >  1:280137/1‑139 (MQ=255)
                                                                   tctATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCACGGTTTGATATACCGAACCCGACAGCAACCGGTACAGTGCTGAGATCCTTCACTGTCCGCATGAACg                                                                      >  1:329035/1‑139 (MQ=255)
                                                                   gctctttttCTGCCGAGCGCACTTCCACCGACGCCACCGGCACAAATTTCATTCATCTTCCTGACCTTGTCGCGGTGTGCTATACAGAACCCGACAGCACCCGGAACAGTGCTGAGATCCTTCACTGTCCGGATTAACg                                                                      >  6:158928/5‑139 (MQ=255)
                                                                               aCGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACgg                                                             <  4:74817/136‑1 (MQ=255)
                                                                               aCGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACgg                                                             <  2:280137/136‑1 (MQ=255)
                                                                               aCGAGCGCAATTCCAACTACGACACCGTAAAAAATTTCATTCATCTTCATGACCTGTTCGGGGTTTTATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATTAACGGGTACACGGAt                                                           <  8:307201/138‑1 (MQ=255)
                                                                                    cgcACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAAtt                                                      >  2:11873/1‑138 (MQ=255)
                                                                                    cgcACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGAGGTTTGATATACCGAAACCGACAGCAACCGGAACAGTTCTGAGATCCTTCACTGTCCGTATGAACGGGTACACGGATGAAtt                                                      >  7:307201/1‑138 (MQ=255)
                                                                                              aCTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       >  5:248655/1‑79 (MQ=255)
                                                                                              aCTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGaa                                                                                                       <  6:248655/79‑1 (MQ=255)
                                                                                                    acacCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGacac                                     >  5:6391/1‑139 (MQ=255)
                                                                                                        cGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACa                                                                                                     <  7:128886/71‑1 (MQ=255)
                                                                                                        cGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACa                                                                                                     >  8:128886/1‑71 (MQ=255)
                                                                                                        cGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGAt                                                           <  5:302545/113‑1 (MQ=255)
                                                                                                        cGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGAt                                                           >  6:302545/1‑113 (MQ=255)
                                                                                                        cGTCAAAAATTTCATTCATCTTCATGACGTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGt                                 <  5:272986/139‑1 (MQ=255)
                                                                                                          tCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa                                                                                                                 <  1:197264/57‑1 (MQ=255)
                                                                                                          tCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACa                                                                                                                 >  2:197264/1‑57 (MQ=255)
                                                                                                          tCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTca                               <  2:57806/139‑1 (MQ=255)
                                                                                                          tCACAAATTTCATTCATCTTCATGACCTGGTCGCGGTTTGATATCCCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTca                               <  2:280060/139‑1 (MQ=255)
                                                                                                          tCAAAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTTATATACCGAACCCGACAGCAACCGGAAGAGTGCTGAGATACTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTca                               <  4:227534/139‑1 (MQ=255)
                                                                                                                 tttCATTCATCTTCATGACATGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTag                        <  3:49801/139‑1 (MQ=255)
                                                                                                                 gttctttcaGCTTCTTGCAGTGTTCACGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTag                        <  2:329035/138‑1 (MQ=255)
                                                                                                                 cttcattcaTCTTCACGACCTGTGCGCGGTTTCATATCCCGAACCCGCCAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGACTTGAACTCATTGCCGACACCGGTCACACCTag                        <  4:272081/138‑1 (MQ=255)
                                                                                                                    cattcatCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTagag                      >  3:338336/1‑138 (MQ=255)
                                                                                                                     attcatCTTCATGACCTGTTCGCGGTTTGATATCCCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAgaga                     <  5:120646/138‑1 (MQ=255)
                                                                                                                         atCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATa                <  1:11873/139‑1 (MQ=255)
                                                                                                                             tCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAACAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATacac             <  8:59692/138‑1 (MQ=255)
                                                                                                                               aTGACCTGTTCGCGGTTTTATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAg           <  6:6391/138‑1 (MQ=255)
                                                                                                                                    ctttccgcgGTTTTATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACg     <  1:192085/134‑1 (MQ=255)
                                                                                                                                       ttCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTc                                <  1:269530/109‑1 (MQ=255)
                                                                                                                                       ttCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTc                                >  2:269530/1‑109 (MQ=255)
                                                                                                                                       ttCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAg  >  5:157842/1‑139 (MQ=255)
                                                                                                                                          |                                                                                                                                       
GTAATCTTCAAACTCCTGCAGCGCTTGATCTCTCGTTTCAGTGCTGATGAGCCGGTCCTTTAGCTCTTCTATTTTTCTGACGAGCGCACTTCCCACTACGACACCGTCACAAATTTCATTCATCTTCATGACCTGTTCGCGGTTTGATATACCGAACCCGACAGCAACCGGAACAGTGCTGAGATCCTTCACTGTCCGGATGAACGGGTACACGGATGAATTGAACTCATTGCGGACACCGGTCACACCTAGAGAAGATACACAGTAGACGAAG  >  CP006881/2189406‑2189679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑