Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,470,510 T→C 7.8% I48V (ATT→GTT)  U712_12610 ← Sporulation‑specific extracellular nuclease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,470,5100TC 7.8% 146.3 / 3.5 52I48V (ATT→GTT) U712_12610Sporulation‑specific extracellular nuclease
Reads supporting (aligned to +/- strand):  ref base T (27/20);  new base C (2/2);  total (30/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ACCGCCATCGGCCACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTGCAGCAAGAAACAGGCCTGCCATC  >  CP006881/2470374‑2470640
                                                                                                                                        |                                                                                                                                  
aCCGCCATCGGCCACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCGCCATCTCTGTCAATGGTAAAAATATCTGGATGTCCCACTGCAATCGCATCCCTAatat                                                                                                                                  >  2:174947/1‑139 (MQ=255)
   gCCATCGGCCACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGAc                                                                                                                               >  7:277844/1‑139 (MQ=255)
    ccATCGGCCACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAAACGCATCCCTAATATGACt                                                                                                                              >  6:113452/1‑139 (MQ=255)
            cACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCACTCGCATCCCTAATATGACTGCCGGttt                                                                                                                      >  4:358613/1‑139 (MQ=255)
            cACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTATGCTCAATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGCCTGCCGGttt                                                                                                                      >  4:358607/1‑139 (MQ=255)
               tCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCg                                                                                                                   >  7:27359/1‑139 (MQ=255)
                   cccGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGAt                                                                                                                >  2:95640/1‑138 (MQ=255)
                         cATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAAGATGACTGCCGGGTGCCGGATAACgaga                                                                                                         >  2:21161/1‑139 (MQ=255)
                           tAGCCCGGCTTGGTCGGGCTTCCCTTTAAAGCTGCCTCCCGCCTTTTGTCTGCTCCATCTCTGGCAATGGTCCAAATATCTGGAGGTCGCTCTGCATTCGCGTCCCGAATATGACTGCCGGTTTCCGGAGAGCGCGAcc                                                                                                       >  7:289463/1‑138 (MQ=255)
                           tAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACa                                                                                                       <  5:186022/139‑1 (MQ=255)
                              cccGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCg                                                                                                    <  1:171822/139‑1 (MQ=255)
                              cccGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCg                                                                                                    <  1:95640/139‑1 (MQ=255)
                              cccGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCACCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTGTCCGGATAACGAGACAGCg                                                                                                    >  1:316146/1‑139 (MQ=255)
                                cGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  <  7:102504/139‑1 (MQ=255)
                                  gCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  <  5:329096/137‑1 (MQ=255)
                                  gCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  >  6:329096/1‑137 (MQ=255)
                                        tCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGCCTGCCGGTTGCGGGATAACGAGACAGCGGAAAATATaa                                                                                          >  6:2420/1‑139 (MQ=255)
                                        tCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATaa                                                                                          >  7:141140/1‑139 (MQ=255)
                                             aTTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  >  2:316609/1‑126 (MQ=255)
                                             aTTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  <  1:316609/126‑1 (MQ=255)
                                                  cTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  <  7:216443/121‑1 (MQ=255)
                                                  cTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGa                                                                                                  >  8:216443/1‑121 (MQ=255)
                                                     tAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATa                                                                              <  4:183099/138‑1 (MQ=255)
                                                       aaGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACg                                                                            <  1:274358/138‑1 (MQ=255)
                                                             cctccCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATg                                                                      <  2:6258/138‑1 (MQ=255)
                                                             cctccCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATg                                                                      <  8:277844/138‑1 (MQ=255)
                                                             cctccCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATg                                                                      <  8:141140/138‑1 (MQ=255)
                                                                        ttGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATc                                                           <  4:136324/138‑1 (MQ=255)
                                                                               cTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATc                                                           <  6:232431/131‑1 (MQ=255)
                                                                               cTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATc                                                           >  5:232431/1‑131 (MQ=255)
                                                                               cTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCgg                                                   <  8:27359/139‑1 (MQ=255)
                                                                               cTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCgg                                                   <  5:113452/139‑1 (MQ=255)
                                                                                         gTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACt                                                                                      >  4:303250/1‑94 (MQ=255)
                                                                                         gTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACt                                                                                      <  3:303250/94‑1 (MQ=255)
                                                                                         gTCAATGGTACAAATATCTGGATGTCACTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGCCAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGCTGTGTTGCGGAACAATTtaa                                         >  8:77540/1‑139 (MQ=255)
                                                                                          tCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTaaa                                         >  4:254793/1‑138 (MQ=255)
                                                                                          tCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTaaa                                         >  2:357063/1‑138 (MQ=255)
                                                                                            aaTGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCGGGTGTCCGGATATCGCGACAGCGGAAAATATAACTCTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGACCCAGTAAGCa                                       >  2:182998/1‑138 (MQ=255)
                                                                                            aaTGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACa                                       >  1:298331/1‑138 (MQ=255)
                                                                                                      atatCTGCATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTCGATCTGTTGCGGAACCATTAAACAAAGAAGAActg                            >  6:16463/1‑139 (MQ=255)
                                                                                                        atCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAActgct                          >  4:64203/1‑139 (MQ=255)
                                                                                                          cTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATc                                                           <  4:291860/104‑1 (MQ=255)
                                                                                                          cTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATc                                                           >  3:291860/1‑104 (MQ=255)
                                                                                                          cTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAActgctg                         >  7:361659/1‑138 (MQ=255)
                                                                                                              aTGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTgcagca                    <  1:36552/139‑1 (MQ=255)
                                                                                                              aTGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGGTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTgcagca                    <  1:174947/139‑1 (MQ=255)
                                                                                                                  cccTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGCGACAGCGGAAAATATAACACTTTGTCATACGAAGCTGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTGCAGCAAGa                 >  4:262783/1‑138 (MQ=255)
                                                                                                                             cGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTGCAGCAAGAAACAGGCCTGc      >  8:78868/1‑138 (MQ=255)
                                                                                                                               cATCCCTAACATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCgg                                                   >  2:141731/1‑91 (MQ=255)
                                                                                                                               cATCCCTAACATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCgg                                                   >  2:136583/1‑91 (MQ=255)
                                                                                                                               cATCCCTAACATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCgg                                                   <  1:141731/91‑1 (MQ=255)
                                                                                                                               cATCCCTAACATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCgg                                                   <  1:136583/91‑1 (MQ=255)
                                                                                                                                 tCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTGCAGCAAGAAACAGGCCTGccatc  >  4:78874/1‑138 (MQ=255)
                                                                                                                                        |                                                                                                                                  
ACCGCCATCGGCCACTCATCCCGGTCATAGCCCGGCTTGGTCGGGATTCCCTTTAAAGATTCCTCCCGCCTTTTGTCTGCTCCATCTCTGTCAATGGTACAAATATCTGGATGTCCCTCTGCAATCGCATCCCTAATATGACTGCCGGTTTCCGGATAACGAGACAGCGGAAAATATAACACTTTGTCATACGAAGATGCGCCTTGGATCTGTTGCGGAACCATTAAACAAAGAAGAACTGCTGCAGCAAGAAACAGGCCTGCCATC  >  CP006881/2470374‑2470640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑