Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,998,644 A→G 10.4% F402S (TTT→TCT)  U712_15405 ← Uncharacterized protein yuaG

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,998,6440AG10.4% 131.7 / 7.1 48F402S (TTT→TCT) U712_15405Uncharacterized protein yuaG
Reads supporting (aligned to +/- strand):  ref base A (20/23);  new base G (3/2);  total (23/25)
Fisher's exact test for biased strand distribution p-value = 6.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

TTCACCGCTTCCTCCTGTATCGACAACGGTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCTCA  >  CP006881/2998514‑2998778
                                                                                                                                   |                                                                                                                                      
ttCACCGCTTCCTCCTGTATCGACAACGGTGATTTTATCAA‑TATTTGAAATAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGcc                                                                                                                                <  4:23204/139‑1 (MQ=255)
 gcACGGCTTCCTCCTGTATCG‑CAACCGTGTTTTTTTTAATTATTTCAAACCGGGGCCGCGGCTGTTTTCGCGTTTCCCGTACGCTTTTTCACAATATAATCGAAACTCGCCGCCTGCCCGTACTGTTCAAAGGCGGcc                                                                                                                                <  2:152693/137‑1 (MQ=255)
               tGTATCGACAACGGTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCAGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCtttt                                                                                                                 >  8:334283/1‑139 (MQ=255)
                   tCGACAACGGTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCt                                                                                                              <  8:89753/138‑1 (MQ=255)
                   tCGACAACGGTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTAAAAGGCGGCCGCAATTTTCTCTTTTTCtt                                                                                                             >  3:63517/1‑139 (MQ=255)
                       aaaCGGTTATTTTATCAA‑TATTTGAAATAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATAATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGc                                                                                                         <  4:63517/138‑1 (MQ=255)
                        aaCGGTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTATCTTTTTATTCCGCt                                                                                                        >  6:266881/1‑139 (MQ=255)
                           ggTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCt                                                                                                     >  5:117896/1‑139 (MQ=255)
                               aTTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCaa                                                                                                 <  2:264350/139‑1 (MQ=255)
                                ttttATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCaa                                                                                                 <  5:266881/138‑1 (MQ=255)
                                     tCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCtt                                                                                           >  8:274309/1‑139 (MQ=255)
                                     tCAA‑TATTTGAAAGAGGTGCCGCTGCTTGGTTCGCGTATTCCGGAAGCATTTTGACAATAATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCCCTTTTTCTTCCGCGTCTGGACGAccgg                                                                                           >  5:231924/1‑137 (MQ=255)
                                          tatTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAg                                                                                       >  1:274653/1‑139 (MQ=255)
                                          tatTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTATCTTTTTATTCCGCTTCTGCAAGACCATTCAg                                                                                       >  2:348990/1‑139 (MQ=255)
                                                 aaGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATa                                                                                <  7:265322/139‑1 (MQ=255)
                                                     ggTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGACAATCGCCGCCTGCCCGTACTGTTCAACGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACct                                                                            <  1:267346/139‑1 (MQ=255)
                                                     ggTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACct                                                                            <  8:36717/139‑1 (MQ=255)
                                                     ggTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACct                                                                            <  2:274653/139‑1 (MQ=255)
                                                      gTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACctc                                                                           <  1:348990/139‑1 (MQ=255)
                                                          cGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCt                                                                       >  2:299920/1‑139 (MQ=255)
                                                               ctTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGt                                                                  >  8:365086/1‑139 (MQ=255)
                                                                  gTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGtct                                                                <  1:299920/138‑1 (MQ=255)
                                                                      cgcgTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCt                                                           >  4:168751/1‑139 (MQ=255)
                                                                             tCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCtt                                                    >  6:372751/1‑139 (MQ=255)
                                                                                 gAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCACAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGAt                                                                                 >  1:153502/1‑106 (MQ=255)
                                                                                 gAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCACAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGAt                                                                                 <  2:153502/106‑1 (MQ=255)
                                                                                           gACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCGAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGc                                       <  6:117896/138‑1 (MQ=255)
                                                                                                   tatCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAg                              <  3:264943/139‑1 (MQ=255)
                                                                                                          aaTCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCt                                                                    <  2:328182/94‑1 (MQ=255)
                                                                                                          aaTCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCt                                                                    >  1:328182/1‑94 (MQ=255)
                                                                                                                       gcgaaCTGTTCAAAGGCGGCAGCAAATTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTATTGCTAGCCAGTCAATTCTTACttttt          <  1:235320/135‑1 (MQ=255)
                                                                                                                       ccGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACttttt          <  2:112743/139‑1 (MQ=255)
                                                                                                                              gTTCAGAGGCGGCCGCAATTTTCTCTTTTTCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCt                                >  8:142932/1‑110 (MQ=255)
                                                                                                                              gTTCAGAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCt                                <  7:142932/110‑1 (MQ=255)
                                                                                                                              gTTCAGAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCt                                >  4:227129/1‑110 (MQ=255)
                                                                                                                              gTTCAGAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCt                                <  3:227129/110‑1 (MQ=255)
                                                                                                                               ttCAGAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCGTCTGCTTTTGCTAGCCCGGCAATTCTTACTTTTTCCGCCt    >  5:322452/1‑137 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTTCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTGGGTTTTTCCGCGTCTGCTTTTTCGAGCCCGTCAATTCTTAATTTTTCCGCCt    >  2:219509/1‑137 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGAt                                                                                 <  5:305328/60‑1 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGAt                                                                                 >  6:305328/1‑60 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACttttt          >  7:86306/1‑131 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACttttt          <  8:86306/131‑1 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCggagaa  >  6:236597/1‑134 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCt    >  1:290108/1‑137 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCt    <  2:290108/137‑1 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCt    <  7:307113/137‑1 (MQ=255)
                                                                                                                               ttCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCt    >  8:49690/1‑137 (MQ=255)
                                                                                                                               gtCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCt    <  5:236597/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                      
TTCACCGCTTCCTCCTGTATCGACAACGGTGATTTTATCAA‑TATTTGAAAGAGGTGCCGCTGCTTGTTTCGCGTATTCCGGAAGCATTTTGACAATCATATCGAAAATCGCCGCCTGCCCGTACTGTTCAAAGGCGGCCGCAATTTTCTCTTTTGCTTCCGCTTCTGCAAGACCTTTCAGGCGGATAACCTCTGCTTCTGTCTCTCCTTTCGCTTTTTCCGCTTCTGCTTTTGCTAGCCCGTCAATTCTTACTTTTTCCGCCTCA  >  CP006881/2998514‑2998778

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑