Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,059,122 C→T 6.3% R62W (CGG→TGG)  U712_15715 → putative ABC transporter ATP‑binding protein YufO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,059,1220CT 6.3% 207.0 / 3.3 63R62W (CGG→TGG) U712_15715putative ABC transporter ATP‑binding protein YufO
Reads supporting (aligned to +/- strand):  ref base C (25/34);  new base T (2/2);  total (27/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

GTATCGTCGCCAATGACAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCGG  >  CP006881/3058985‑3059246
                                                                                                                                         |                                                                                                                            
gTATCGTCGCCAATGACAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCg                                                                                                                             >  8:327679/1‑139 (MQ=255)
   tcgtcgCCAATGACAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCATTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGt                                                                                                                          >  7:335662/1‑139 (MQ=255)
        gCCAATGACAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTgcgc                                                                                                                      <  4:139690/138‑1 (MQ=255)
             tGACAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCgcggc                                                                                                                   >  5:46317/1‑136 (MQ=255)
               aCAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCgaga                                                                                                               <  6:46317/138‑1 (MQ=255)
                 aaTATCAATCTTCAAGTAAAAAAACGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAgg                                                                                                            <  6:162401/139‑1 (MQ=255)
                   tatCAATCTTCAAGTCAAAAAAGGCTAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCATTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCTGCGAGAAGGTg                                                                                                          <  8:19844/139‑1 (MQ=255)
                   tatCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTg                                                                                                          >  5:78911/1‑139 (MQ=255)
                        aTCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACAt                                                                                                     >  2:1892/1‑139 (MQ=255)
                           ttCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCaa                                                                                                  >  6:209967/1‑139 (MQ=255)
                           ttCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATAAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGTTGAAATTCGGGTGCGCGGCGAGAAGCTGCACATCaa                                                                                                  >  4:347170/1‑139 (MQ=255)
                             cAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCaa                                                                                                  >  3:149799/1‑137 (MQ=255)
                             cAAGTCAAAAAACGAGAAATACACGCTTTGCTCGGTGAAAACGGAGCGGTGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGTGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCaa                                                                                                  <  3:347170/137‑1 (MQ=255)
                                gTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGcc                                                                                             <  4:182501/139‑1 (MQ=255)
                                             aaaTACACGCGTTGCTCGGTGAAAACGGCGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGaa                                                                                <  8:269104/139‑1 (MQ=255)
                                             aaaTACACGCGGTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGaa                                                                                <  4:321800/139‑1 (MQ=255)
                                             aaaTACACGCGGTGCTCCGTTAAAAAGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGaa                                                                                <  4:326876/139‑1 (MQ=255)
                                                   ccgcgTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCt                                                                          <  3:204227/138‑1 (MQ=255)
                                                   acGCGTTGCTCGTTGAAAAGGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGTGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCt                                                                          <  1:291029/139‑1 (MQ=255)
                                                   acGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCt                                                                          <  7:237186/139‑1 (MQ=255)
                                                   acGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGCGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCt                                                                          <  8:344988/139‑1 (MQ=255)
                                                   acGCGGTGCTCGGTGAAAACGGTTCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCt                                                                          <  6:232644/139‑1 (MQ=255)
                                                    cgcgTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCtt                                                                         <  5:209967/139‑1 (MQ=255)
                                                      cgTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCttgg                                                                       <  7:187477/139‑1 (MQ=255)
                                                          gCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTTGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCttggaatt                                                                   >  3:292998/1‑139 (MQ=255)
                                                          gCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTTGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCttggaatt                                                                   >  3:287835/1‑139 (MQ=255)
                                                          gCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTg                                                                                                          <  3:74333/100‑1 (MQ=255)
                                                          gCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTg                                                                                                          >  4:74333/1‑100 (MQ=255)
                                                          gCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCttggaatt                                                                   >  2:354393/1‑139 (MQ=255)
                                                          gCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCttggaatt                                                                   >  8:116825/1‑139 (MQ=255)
                                                               gTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTtggaattggaat                                                              <  3:145280/139‑1 (MQ=255)
                                                               gTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTtggaattggaat                                                              >  5:290278/1‑139 (MQ=255)
                                                                  aaaaCGGTGCGGGGAAATCAACATTGATGAATGGCCTTTGCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATgg                                                            <  6:260261/138‑1 (MQ=255)
                                                                       ggAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCAcc                                                      <  7:30509/139‑1 (MQ=255)
                                                                       ggAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCAcc                                                      <  7:38339/139‑1 (MQ=255)
                                                                        gAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCa                                                     <  4:135354/139‑1 (MQ=255)
                                                                        gAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCa                                                     <  1:1892/139‑1 (MQ=255)
                                                                         aGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAg                                                    <  5:74953/139‑1 (MQ=255)
                                                                         aGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAg                                                    <  6:156567/139‑1 (MQ=255)
                                                                         aGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAg                                                    <  8:123811/139‑1 (MQ=255)
                                                                         aGCGGGCAAATCAACATTGATGAATGTCCTTTTCGGGCTGGATCAGCCGGAGCGGGGGGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAg                                                    <  5:24300/139‑1 (MQ=255)
                                                                          gCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGc                                                   >  4:82530/1‑139 (MQ=255)
                                                                           cGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTTGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCa                                                  <  4:292998/139‑1 (MQ=255)
                                                                           cGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTTGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCa                                                  <  4:287835/139‑1 (MQ=255)
                                                                                       cATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCttgt                                      >  7:289646/1‑139 (MQ=255)
                                                                                           gatgaATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGa                                   <  7:327679/138‑1 (MQ=255)
                                                                                           gatgaATGTCCTTTTCGGGCTGGATCAGCCGGAGCGGGGGGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGa                                   <  4:361638/138‑1 (MQ=255)
                                                                                            atgaATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGaca                                 >  1:2236/1‑139 (MQ=255)
                                                                                                 tGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGca                                                                                                        <  6:61045/63‑1 (MQ=255)
                                                                                                 tGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGca                                                                                                        >  5:61045/1‑63 (MQ=255)
                                                                                                 tGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCtt                             >  8:280640/1‑138 (MQ=255)
                                                                                                        ttCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGt                       <  7:116825/137‑1 (MQ=255)
                                                                                                            ggCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGa                 <  5:46244/139‑1 (MQ=255)
                                                                                                             gCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGa                 >  6:46244/1‑138 (MQ=255)
                                                                                                                 tATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAata            >  3:145237/1‑139 (MQ=255)
                                                                                                                     aGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACCGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAAtatta         <  4:145237/138‑1 (MQ=255)
                                                                                                                           aGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCgg  >  7:271254/1‑139 (MQ=255)
                                                                                                                           aGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCgg  >  7:31035/1‑139 (MQ=255)
                                                                                                                           aGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCgg  >  5:169743/1‑139 (MQ=255)
                                                                                                                           aGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCgg  >  3:198285/1‑139 (MQ=255)
                                                                                                                           aGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCgg  >  2:144973/1‑139 (MQ=255)
                                                                                                                           aGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGAGCACCa                                                     >  8:287402/1‑88 (MQ=255)
                                                                                                                           aGCGGGGGGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCa                                                     <  7:287402/88‑1 (MQ=255)
                                                                                                                                         |                                                                                                                            
GTATCGTCGCCAATGACAATATCAATCTTCAAGTCAAAAAAGGCGAAATACACGCGTTGCTCGGTGAAAACGGAGCGGGGAAATCAACATTGATGAATGTCCTTTTCGGGCTGTATCAGCCGGAGCGGGGTGAAATTCGGGTGCGCGGCGAGAAGGTGCACATCAACAGCCCGAACAAAGCGAATGACCTTGGAATTGGAATGGTGCACCAGCACTTTATGCTTGTTGACACCTTTACAGTTGCTGAAAATATTATTCTCGG  >  CP006881/3058985‑3059246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑