Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,498,073	C→A	12.4%	D68Y (GAT→TAT)	U712_17935 ←	putative poly(glycerol‑phosphate) alpha‑glucosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,498,073	0	C	A	12.4%	224.5 / 2.9	90	D68Y (GAT→TAT)	U712_17935	putative poly(glycerol‑phosphate) alpha‑glucosyltransferase
Reads supporting (aligned to +/- strand):  ref base C (49/27);  new base A (2/11);  total (51/39)
Fisher's exact test for biased strand distribution p-value = 1.53e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTATCTGGTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGTCCAC  >  CP006881/3497944‑3498204                                                                                                                                  |                                                                                                                                     ttATCTGGTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTg                                                                                                                              <  8:161740/138‑1 (MQ=255)     cTGGTCAAGGCCTATCATTTTTTCTTATGATC‑TTTCCCATTTGTTCTAACACTTAAAAAATCATCTAATAGATTAATAACACTTGTTAATTTTTTATAAATCTTACCGTTGCTGTACATTTCATCAATTTTTGAAG‑Ac                                                                                                                        <  2:76091/138‑1 (MQ=255)       ggTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACaa                                                                                                                      >  8:236173/1‑139 (MQ=255)        gTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTTTTAATTTTTTATCAATATTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑Ac                                                                                                                        <  5:339084/136‑1 (MQ=255)        gTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTTTAAAATTCATAAATTTTTgacg‑ac                                                                                                                        <  3:35299/136‑1 (MQ=255)        gTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCATTTTTTGAAG‑Ac                                                                                                                        <  4:260403/136‑1 (MQ=255)             ggCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTa                                                                                                                 >  3:118687/1‑138 (MQ=255)                  aTCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCa                                                                                                            >  1:89057/1‑138 (MQ=255)                  aTCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCa                                                                                                            >  3:314206/1‑138 (MQ=255)                  aTCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCa                                                                                                            >  8:368005/1‑138 (MQ=255)                  aTCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTATAAATCa                                                                                                            >  1:151809/1‑138 (MQ=255)                  aTCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAAAAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCTTAAATTTTTGAAG‑ACAACTCTAAATCa                                                                                                            >  1:102363/1‑138 (MQ=255)                   tCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCaa                                                                                                           >  1:31509/1‑138 (MQ=255)                   tCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCaa                                                                                                           >  2:204059/1‑138 (MQ=255)                   tCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCCAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCaa                                                                                                           >  7:321423/1‑138 (MQ=255)                       ttcttcGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAaaacg                                                                                                       >  5:54642/1‑138 (MQ=255)                        tcttcGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAaaacga                                                                                                      >  6:145696/1‑138 (MQ=255)                        tcttcGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAaaacga                                                                                                      >  8:116981/1‑138 (MQ=255)                        tcttcGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAaaacga                                                                                                      >  5:147953/1‑138 (MQ=255)                        tcttcGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAaaacga                                                                                                      >  4:10899/1‑138 (MQ=255)                        tcttcGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAaaacga                                                                                                      >  1:157801/1‑138 (MQ=255)                             gTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGt                                                                                                 <  8:40793/138‑1 (MQ=255)                             cttttaTCTTTACCCTTTTTTTCTAACAATTAAAAAAATATGCAATTTATAAATACCAATTGTTATTTTTTTATCAATCTTACCGTTGCTGTACAATTCATAAATTTTTGATC‑ACAAATCTAAATCAAAACGAAACGt                                                                                                 <  1:354470/133‑1 (MQ=255)                                        tCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGgt                                                                                     >  3:29660/1‑139 (MQ=255)                                         cccTTTTGTTCTAACACTAAAAAAATCATCTAATAGATTAATAACACTTGTTAATTTTTAATCATTCTTACCGTGGCTGAACAATTCAACAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGgt                                                                                     <  1:137818/138‑1 (MQ=255)                                          ccATTTGTTCTAACACTTAAAAAATCATCGAATATATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGgtgt                                                                                   <  7:8060/139‑1 (MQ=255)                                           cATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGtt                                                                                  <  3:56740/139‑1 (MQ=255)                                            aTTTTTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGttt                                                                                 <  8:367614/139‑1 (MQ=255)                                            aTTTGTTCTAACACTTAAAAACTCATCTAATATATTAAAAACACTTGTTAATTTTTTATCAATCTTCCCGTTGCTGAACAATACATAATTTTTTCAAC‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGttt                                                                                 <  7:140894/139‑1 (MQ=255)                                            aTTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGttt                                                                                 <  4:297984/139‑1 (MQ=255)                                            aTTTGTACAAAAACTTAAAAAATCATCTAATATATTAAAAACACTTGTTAATTTTTTAACAATATAACCGTTGTTGTAAAATTCATCAATTTTTTAAC‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGttt                                                                                 <  4:28592/139‑1 (MQ=255)                                             tttGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGtttt                                                                                >  6:352795/1‑139 (MQ=255)                                             tttGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGtttt                                                                                >  3:28592/1‑139 (MQ=255)                                             tttGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGtttt                                                                                <  6:264106/139‑1 (MQ=255)                                             tttGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACAGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAAAGTAAAGAAAAAGGTGttat                                                                                >  6:129127/1‑137 (MQ=255)                                                 ttCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTTAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGt                                                                             >  6:270256/1‑138 (MQ=255)                                                 ttCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGt                                                                             >  1:206349/1‑138 (MQ=255)                                                  tCTAAAACTTAAAAAATCATCTAATATATTAATAACAGTTGTTATTTTTTTATAATTATTACCGTTGCTTTAAAATTCATCATTTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGtt                                                                            <  4:348780/138‑1 (MQ=255)                                                   atAACACTTAAAAAATCATCTAATAGATTAATAACACTTGTTAATTTTTTATCAATCTTACCGTTGCTGTAAAATTCATAAATTTTTTAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTa                                                                           <  2:89057/137‑1 (MQ=255)                                                      acacTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACAct                                                                       >  5:123572/1‑139 (MQ=255)                                                         cTTAAAAAATAATCGAATATATTAATAACAATTGTTAATTTTTTATCAATTTTACCTTTGCTTTAAATTTCATCAATTTTTTAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACtctt                                                                    <  4:118687/139‑1 (MQ=255)                                                           tAAAAACTCATCTAATAGATTAATAACACTTGTGAATTTTTAATCAATCTTACCGTTGCTGAACAATTCATCAATTTTTGAAC‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACtcttct                                                                  <  3:284204/139‑1 (MQ=255)                                                           tAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAACCGTTAAGAAAAAGGTGTTTTGGTTACACtcttca                                                                  >  5:16324/1‑138 (MQ=255)                                                           tAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACtcttct                                                                  >  5:86135/1‑139 (MQ=255)                                                           tAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACtcttc                                                                   >  5:188731/1‑138 (MQ=255)                                                            aaaaaaTCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACtcttct                                                                  <  7:368005/138‑1 (MQ=255)                                                            aaaaaaTCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTtctc                                                                 >  3:348780/1‑139 (MQ=255)                                                            aaaaaaTCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTtctc                                                                 >  1:27923/1‑139 (MQ=255)                                                                aaTCATCGAATAGATAAAAACCACTTGTGAATTTTTTATCAATCTTACGGTTCCTGTACAATTCATAAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCaaa                                                             <  4:162232/139‑1 (MQ=255)                                                                    atcGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAaataat                                                         >  1:95865/1‑139 (MQ=255)                                                                          tattataataaCAATTGTTAATTTTTTATCAATATTACCGTTGCTGTAAAATTCATCAATTTTTCAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   <  5:212749/134‑1 (MQ=255)                                                                          tAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   <  7:236173/139‑1 (MQ=255)                                                                          tAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   <  7:82594/139‑1 (MQ=255)                                                                          tAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   <  5:145696/139‑1 (MQ=255)                                                                           attttAATAAAACTTGTTAATTTTTTATCAATCTTACCTTTGATTTACAATTAATAAATTTTTGAAG‑AAAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   <  8:2800/135‑1 (MQ=255)                                                                            taTTAATAACAATTTTTAATTTTTTATCAATATTACCTTTGGTGTAAAATTCATAATTTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACg                                                 <  2:311953/138‑1 (MQ=255)                                                                            taTTAAAAAAAATTGTTAATTTTTAATAAATATTACGGTTGCTTTACAATAAATAAATTTTTCAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACg                                                 <  4:29660/138‑1 (MQ=255)                                                                             aTTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaaag                                                 >  3:194540/1‑136 (MQ=255)                                                                             aTTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACg                                                 >  3:64728/1‑138 (MQ=255)                                                                              ttAATAACACTTTTTAATTTTTTATCAATCTTACCGTTTCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGc                                                <  3:155677/138‑1 (MQ=255)                                                                                 aaaaCACATGTGCATTTTTTATAAAATCTACCGCTTCTTTAACAATCATTAATATTTGAAGCACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaat                                             <  2:92998/137‑1 (MQ=255)                                                                                       cTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaataataat                                       >  3:2344/1‑138 (MQ=255)                                                                                       cTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaataataat                                       >  1:309519/1‑138 (MQ=255)                                                                                       cTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaataataat                                       >  6:145385/1‑138 (MQ=255)                                                                                       cTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaataataat                                       >  4:330816/1‑138 (MQ=255)                                                                                       cTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATg                                      >  5:317707/1‑139 (MQ=255)                                                                                        ttGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATgc                                     >  1:311953/1‑138 (MQ=255)                                                                                          ttcaaTTTTTTATAAATATTCCCTTTGCTTAACAATTCATCAATTTTTTACC‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGAt                                    <  3:10899/135‑1 (MQ=255)                                                                                          gtgAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGaaaa                                                                                         <  3:30965/85‑1 (MQ=255)                                                                                          gtgAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGaaaa                                                                                         >  4:30965/1‑85 (MQ=255)                                                                                                 ttttATAAATCTTACCTTTGTTTTAAAATACTTAATTTTTTTACG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCa                             <  2:206349/138‑1 (MQ=255)                                                                                                         aTCTAACCGGTGCTGTAAAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCAt                    <  2:297068/139‑1 (MQ=255)                                                                                                          tCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAAATCTAAATCAAAAAGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTATAAAAATAAATTTGAAAGAAATAATAATGATATCGTCAGACAGCAAt                    >  5:310902/1‑138 (MQ=255)                                                                                                           cTTACCGTTGCTGTACAATTCATAAATTTTTGAAG‑ACAACTCTAAATCATAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATaa                  <  7:14214/139‑1 (MQ=255)                                                                                                            ttACCGTTGCTGTACAATTCATAAATTTTTTACG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATaa                  <  2:273623/138‑1 (MQ=255)                                                                                                                    gCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACtt            <  1:16961/136‑1 (MQ=255)                                                                                                                    gCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACtt            >  2:16961/1‑136 (MQ=255)                                                                                                                    gCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTgg          >  1:273623/1‑138 (MQ=255)                                                                                                                    gCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTgg          >  7:89431/1‑138 (MQ=255)                                                                                                                    gCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTgg          >  5:122663/1‑138 (MQ=255)                                                                                                                    gCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAAAAATAATAATTACTTCAGACCGCAATAATTACTTgg          >  8:354214/1‑138 (MQ=255)                                                                                                                    gCTGTACAATTCATAAATATTTGAAG‑ACAAATCTAAATAAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAAAGAAATAATAATGATTTCGTCAGACCGCCATAATTAATTgc          >  5:257657/1‑137 (MQ=255)                                                                                                                    gCTGTACAATTAATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACAGCCAAAATAACTTgg          >  6:191728/1‑138 (MQ=255)                                                                                                                        tACAATTCATCAATTTTTGACG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGGGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGt      <  2:31509/138‑1 (MQ=255)                                                                                                                             ttCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   >  5:366941/1‑88 (MQ=255)                                                                                                                             ttCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGaa                                                   <  6:366941/88‑1 (MQ=255)                                                                                                                             ttCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGTccac  >  2:262104/1‑137 (MQ=255)                                                                                                                             ttCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGTccac  >  7:98720/1‑137 (MQ=255)                                                                                                                             ttCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGTccac  >  5:160364/1‑137 (MQ=255)                                                                                                                             ttCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGTccac  >  6:237114/1‑137 (MQ=255)                                                                                                                                atcaATTTTTTAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGc                                                <  4:372533/88‑1 (MQ=255)                                                                                                                                atcaATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGc                                                >  3:372533/1‑88 (MQ=255)                                                                                                                                  caATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaataata                                         <  3:63906/93‑1 (MQ=255)                                                                                                                                  caATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCaataata                                         >  4:63906/1‑93 (MQ=255)                                                                                                                                  |                                                                                                                                     TTATCTGGTCAAGGCCTATCCTTTCTTCGTATGATCTTTTCCCATTTGTTCTAACACTTAAAAAATCATCGAATAGATTAATAACACTTGTGAATTTTTTATCAATCTTACCGTTGCTGTACAATTCATCAATTTTTGAAG‑ACAACTCTAAATCAAAACGAAACGTTAAGAAAAAGGTGTTTTGGTTACACTCTTCTCCAAATAATTTTGAACGCAATAATAATGATTTCGTCAGACCGCCATAATTACTTGGCAGTCCAC  >  CP006881/3497944‑3498204

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑