breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 10,620 A→T 5.2% intergenic (‑126/+23) satP ← / ← yaaW succinate‑acetate transporter/UPF0174 family protein
RA 53,379 T→C 5.1% E13G (GAG→GGG)  pdxA ← 4‑hydroxy‑L‑threonine phosphate dehydrogenase, NAD‑dependent
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 299,984 T→G 8.9% E317D (GAA→GAC paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 318,651 T→A 5.1% intergenic (‑84/+25) rclB ← / ← rclA reactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 318,652 T→A 5.0% intergenic (‑85/+24) rclB ← / ← rclA reactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 318,654 G→A 5.0% intergenic (‑87/+22) rclB ← / ← rclA reactive chlorine species (RCS) stress resistance periplasmic protein/reactive chlorine stress species (RCS) resistance protein; pyridine nucleotide‑dependent disulfide oxidoreductase family
RA 349,717 T→C 6.9% intergenic (+145/‑295) prpB → / → prpC 2‑methylisocitrate lyase/2‑methylcitrate synthase
RA 406,656 G→A 5.2% T84T (ACG→ACA aroL → shikimate kinase II
RA 473,057 A→G 5.3% D31G (GAC→GGC)  amtB → ammonium transporter
RA 481,225 T→G 5.2% intergenic (‑517/+29) tomB ← / ← acrB Hha toxicity attenuator; conjugation‑related protein/multidrug efflux system protein
RA 495,026 A→T 5.9% intergenic (+16/‑94) recR → / → htpG gap repair protein/protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
RA 495,027 T→G 5.9% intergenic (+17/‑93) recR → / → htpG gap repair protein/protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
RA 495,028 C→A 5.8% intergenic (+18/‑92) recR → / → htpG gap repair protein/protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
RA 495,029 A→T 5.8% intergenic (+19/‑91) recR → / → htpG gap repair protein/protein refolding molecular co‑chaperone Hsp90, Hsp70‑dependent; heat‑shock protein; ATPase
RA 574,494 A→G 7.6% intergenic (+155/+35) quuD → / ← nmpC DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 574,495 A→T 7.6% intergenic (+156/+34) quuD → / ← nmpC DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 574,496 C→T 7.6% intergenic (+157/+33) quuD → / ← nmpC DLP12 prophage; putative antitermination protein/DLP12 prophage; truncated outer membrane porin (pseudogene);IS, phage, Tn; Phage or Prophage Related; outer membrane porin protein; locus of qsr prophage
RA 644,042 C→T 5.0% intergenic (‑75/+155) rnk ← / ← rna regulator of nucleoside diphosphate kinase/ribonuclease I
RA 645,069 G→A 5.3% intergenic (‑66/+48) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 645,070 G→A 5.2% intergenic (‑67/+47) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 645,073 T→C 5.1% intergenic (‑70/+44) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 645,074 T→C 5.1% intergenic (‑71/+43) rna ← / ← citT ribonuclease I/citrate/succinate antiporter; citrate carrier
RA 659,204 C→T 8.5% intergenic (+54/+47) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,205 A→T 9.2% intergenic (+55/+46) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,206 A→T 9.3% intergenic (+56/+45) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 659,207 A→G 9.4% intergenic (+57/+44) tatE → / ← lipA TatABCE protein translocation system subunit/lipoate synthase
RA 710,170 C→T 6.4% intergenic (+54/+30) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 710,173 A→G 6.2% intergenic (+57/+27) chiQ → / ← fur chitosugar‑induced verified lipoprotein/ferric iron uptake regulon transcriptional repressor; autorepressor
RA 729,376 G→T 6.0% intergenic (+36/‑207) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
RA 729,377 A→T 6.0% intergenic (+37/‑206) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
RA 729,378 A→T 6.0% intergenic (+38/‑205) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
RA 729,379 A→C 5.6% intergenic (+39/‑204) ybfA → / → rhsC DUF2517 family protein/Rhs protein with putative toxin domain; putative neighboring cell growth inhibitor
RA 760,167 G→A 5.8% A488T (GCT→ACT)  sucA → 2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
RA 770,187 T→C 5.2% L737P (CTG→CCG)  mngB → alpha‑mannosidase
RA 781,238 T→A 6.2% intergenic (+16/‑131) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,254 A→T 8.1% intergenic (+32/‑115) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 781,269 C→T 5.2% intergenic (+47/‑100) lysY → / → lysZ tRNA‑Lys/tRNA‑Lys
RA 802,888 C→T 6.5% I334I (ATC→ATT ybhI → putative DASS family tricarboxylate or dicarboxylate transporter
RA 838,930 T→A 8.5% Y93F (TAT→TTT)  ybiX ← Fe(II)‑dependent oxygenase superfamily protein
RA 852,372 A→G 5.4% F76L (TTC→CTC)  opgE ← OPG biosynthetic transmembrane phosphoethanolamine transferase
RA 933,500 G→A 5.1% G93S (GGT→AGT)  ftsK → DNA translocase at septal ring sorting daughter chromsomes
RA 1,011,009 C→T 5.2% A349V (GCC→GTC)  uup → replication regulatory ABC‑F family DNA‑binding ATPase
RA 1,099,456 C→A 6.9% S193Y (TCT→TAT)  ycdX → alkaline phosphatase
RA 1,147,690 G→A 5.7% A24T (GCA→ACA) ‡ plsX → putative phosphate acyltransferase
RA 1,147,691 C→T 5.7% A24V (GCA→GTA) ‡ plsX → putative phosphate acyltransferase
RA 1,147,692 A→G 5.7% A24A (GCA→GCG) ‡ plsX → putative phosphate acyltransferase
RA 1,147,693 T→C 5.5% L25L (TTG→CTG)  plsX → putative phosphate acyltransferase
RA 1,457,532 A→C 5.0% V12V (GTA→GTC paaF → 2,3‑dehydroadipyl‑CoA hydratase
RA 1,466,336 C→G 5.3% pseudogene (945/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,345 T→C 6.9% pseudogene (954/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,515,338 G→T 7.5% G193* (GGA→TGA)  ydcV → putative ABC transporter permease
RA 1,688,120 C→T 6.1% V86V (GTG→GTA fumA ← fumarate hydratase (fumarase A), aerobic Class I
RA 1,708,709 T→A 5.8% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,792,242 T→A 5.0% intergenic (‑222/+25) ydiV ← / ← nlpC anti‑FlhD4C2 factor, inactive EAL family phosphodiesterase/putative C40 clan peptidase lipoprotein
RA 1,807,316 G→A 7.8% intergenic (+17/‑84) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein
RA 1,807,317 C→T 7.8% intergenic (+18/‑83) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein
RA 1,807,318 A→G 7.9% intergenic (+19/‑82) pfkB → / → ydiZ 6‑phosphofructokinase II/uncharacterized protein
RA 1,864,763 A→T 7.5% intergenic (+29/+19) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
RA 1,864,768 T→C 6.8% intergenic (+34/+14) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,009,731 A→G 5.8% intergenic (+19/‑90) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein
RA 2,009,732 C→G 5.7% intergenic (+20/‑89) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein
RA 2,009,733 C→T 5.7% intergenic (+21/‑88) yedF → / → yedK putative TusA family sulfurtransferase/DUF159 family protein
RA 2,019,460 G→A 100% V359I (GTC→ATC)  fliK → flagellar hook‑length control protein
RA 2,079,004 G→C 10.6% intergenic (+73/+28) yoeF → / ← yeeX pseudogene, CP4‑44 putative prophage remnant;Phage or Prophage Related/UPF0265 family protein
RA 2,104,190 T→C 8.4% Y107C (TAT→TGT)  wbbK ← lipopolysaccharide biosynthesis protein
RA 2,129,974 A→G 5.8% F302S (TTT→TCT)  wcaD ← putative colanic acid polymerase
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,210,853 A→G 5.3% pseudogene (1754/2001 nt) yehQ → pseudogene
RA 2,292,341 G→A 5.7% Q24* (CAG→TAG)  ccmH ← heme lyase, CcmH subunit
RA 2,304,649 C→T 14.3% intergenic (+256/+459) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,304,988 T→C 9.4% intergenic (+595/+120) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,305,013 A→G 5.4% intergenic (+620/+95) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
JC JC 2,375,828 IS186 (+) +4 bp 100% coding (132‑135/963 nt) menC ← O‑succinylbenzoyl‑CoA synthase
RA 2,440,295 A→T 7.2% intergenic (‑175/+90) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,440,296 T→G 7.0% intergenic (‑176/+89) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,440,297 C→A 7.0% intergenic (‑177/+88) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,440,298 A→T 7.1% intergenic (‑178/+87) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,460,614 C→T 7.1% intergenic (‑145/+36) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,460,618 C→T 6.8% intergenic (‑149/+32) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,460,619 A→T 6.8% intergenic (‑150/+31) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,460,620 A→G 6.8% intergenic (‑151/+30) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,460,624 A→G 6.7% intergenic (‑155/+26) fadI ← / ← yfcZ beta‑ketoacyl‑CoA thiolase, anaerobic, subunit/UPF0381 family protein
RA 2,470,427 C→A 26.8% Q433K (CAA→AAA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
RA 2,503,518 G→T 8.4% A323D (GCC→GAC) ‡ fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,503,519 C→T 7.8% A323T (GCC→ACC) ‡ fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,503,522 A→G 8.1% Y322H (TAT→CAT)  fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,503,523 A→C 9.4% R321R (CGT→CGG fryA ← putative PTS enzyme: Hpr, enzyme I and IIA components
RA 2,521,344 C→T 7.5% intergenic (+16/+249) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,521,346 A→T 7.2% intergenic (+18/+247) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,521,348 A→G 7.6% intergenic (+20/+245) lysV → / ← xapR tRNA‑Lys/transcriptional activator of xapAB
RA 2,561,206 A→T 5.2% intergenic (+308/‑162) yffL → / → yffM CPZ‑55 prophage; uncharacterized protein/CPZ‑55 prophage; uncharacterized protein
RA 2,622,348:1 +A 5.2% coding (115/639 nt) purN → phosphoribosylglycinamide formyltransferase 1
RA 2,622,354 Δ1 bp 5.4% coding (121/639 nt) purN → phosphoribosylglycinamide formyltransferase 1
RA 2,662,505 G→T 5.9% intergenic (‑374/+78) iscR ← / ← trmJ isc operon transcriptional repressor; suf operon transcriptional activator; oxidative stress‑ and iron starvation‑inducible; autorepressor/tRNA mC32,mU32 2'‑O‑methyltransferase, SAM‑dependent
RA 2,663,482 A→C 7.1% K14T (AAG→ACG)  suhB → inositol monophosphatase
RA 2,666,972 C→T 5.6% W292* (TGG→TAG)  hcaT ← putative 3‑phenylpropionic transporter
RA 2,666,978 A→G 5.5% L290S (TTG→TCG) ‡ hcaT ← putative 3‑phenylpropionic transporter
RA 2,666,979 A→T 5.3% L290M (TTG→ATG) ‡ hcaT ← putative 3‑phenylpropionic transporter
RA 2,666,980 C→T 5.3% A289A (GCG→GCA hcaT ← putative 3‑phenylpropionic transporter
RA 2,666,986 A→G 5.5% T287T (ACT→ACC hcaT ← putative 3‑phenylpropionic transporter
RA 2,726,043 G→T 5.8% intergenic (‑297/+26) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,726,044 T→G 5.5% intergenic (‑298/+25) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,726,045 C→A 5.9% intergenic (‑299/+24) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,726,046 A→C 5.7% intergenic (‑300/+23) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 2,817,916 T→G 6.0% intergenic (‑56/+143) argQ ← / ← argZ tRNA‑Arg/tRNA‑Arg
RA 2,818,145 T→C 6.4% intergenic (‑10/+53) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,818,149 T→C 6.6% intergenic (‑14/+49) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,907,998 A→T 7.2% intergenic (‑57/+31) eno ← / ← pyrG enolase/CTP synthetase
RA 2,980,742 A→G 7.1% intergenic (‑107/+22) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter
RA 2,980,744 A→T 7.1% intergenic (‑109/+20) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter
RA 2,980,746 C→T 7.3% intergenic (‑111/+18) ygeA ← / ← araE Asp/Glu_racemase family protein/arabinose transporter
RA 2,987,103 C→T 9.1% intergenic (+27/‑433) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator
RA 2,987,104 T→A 10.1% intergenic (+28/‑432) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator
RA 2,987,105 A→G 9.4% intergenic (+29/‑431) yqeG → / → yqeH putative transporter/putative LuxR family transcriptional regulator
RA 3,042,403 A→T 5.7% intergenic (+110/+86) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
RA 3,042,404 A→C 5.8% intergenic (+111/+85) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
RA 3,042,410 T→G 5.7% intergenic (+117/+79) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
RA 3,042,411 C→A 5.7% intergenic (+118/+78) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
RA 3,042,417 G→T 5.6% intergenic (+124/+72) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
RA 3,042,418 A→T 5.5% intergenic (+125/+71) ygfZ → / ← yqfA iron‑sulfur cluster repair protein, plumbagin resistance/hemolysin III family HylIII inner membrane protein
RA 3,046,050 T→G 11.1% intergenic (‑149/+118) ygfF ← / ← gcvP putative NAD(P)‑dependent oxidoreductase/glycine decarboxylase, PLP‑dependent, subunit P of glycine cleavage complex
RA 3,092,842 G→A 5.8% intergenic (+14/‑95) gshB → / → yqgE glutathione synthetase/uncharacterized protein
RA 3,156,590 C→A 6.8% intergenic (+72/‑33) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,156,591 T→G 6.8% intergenic (+73/‑32) yqhD → / → dkgA aldehyde reductase, NADPH‑dependent/2,5‑diketo‑D‑gluconate reductase A
RA 3,183,345:1 +T 5.0% intergenic (+22/+468) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,239,609 T→G 11.9% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,239,610 C→G 7.8% intergenic (+65/‑334) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,239,611 C→A 7.6% intergenic (+66/‑333) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter
RA 3,269,956 T→C 6.2% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,269,969 A→C 5.7% intergenic (+367/+247) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,080 C→T 5.5% intergenic (+478/+136) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,081 G→T 5.5% intergenic (+479/+135) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,082 A→C 9.6% intergenic (+480/+134) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,083 A→G 5.3% intergenic (+481/+133) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,270,143 C→T 5.1% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,283,652 C→T 5.7% D170D (GAC→GAT kbaY → tagatose 6‑phosphate aldolase 1, kbaY subunit
RA 3,313,207 G→A 5.1% intergenic (‑29/+135) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,313,208 T→A 5.2% intergenic (‑30/+134) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,313,209 T→C 5.2% intergenic (‑31/+133) rbfA ← / ← infB 30s ribosome binding factor/translation initiation factor IF‑2
RA 3,324,952 A→G 5.6% intergenic (‑41/+49) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,955 A→G 5.7% intergenic (‑44/+46) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,956 C→T 5.7% intergenic (‑45/+45) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,324,959 C→T 5.8% intergenic (‑48/+42) folP ← / ← ftsH 7,8‑dihydropteroate synthase/protease, ATP‑dependent zinc‑metallo
RA 3,328,182 A→T 6.9% intergenic (+99/+57) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,328,183 T→A 6.9% intergenic (+100/+56) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,328,184 A→T 6.6% intergenic (+101/+55) yhbY → / ← greA RNA binding protein associated with pre‑50S ribosomal subunits/transcript cleavage factor
RA 3,392,189 T→G 5.2% intergenic (‑161/+269) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein
RA 3,392,190 T→C 5.4% intergenic (‑162/+268) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein
RA 3,504,855 T→G 9.0% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
RA 3,507,076 G→C 5.5% L87L (CTC→CTG yhfT ← inner membrane protein
RA 3,557,462 C→A 5.6% E206D (GAG→GAT rtcB ← RNA‑splicing ligase
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,569,442 C→T 5.2% E626E (GAG→GAA glgX ← glycogen debranching enzyme
RA 3,578,609 T→G 5.3% I40L (ATT→CTT)  gntR ← d‑gluconate inducible gluconate regulon transcriptional repressor
RA 3,608,721 A→C 9.9% intergenic (+72/+30) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
RA 3,642,358 T→A 5.4% intergenic (+27/+22) dtpB → / ← rsmJ dipeptide and tripeptide permease B/16S rRNA m(2)G1516 methyltransferase, SAM‑dependent
RA 3,781,126 G→T 7.3% intergenic (+109/‑89) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,132 C→G 7.4% intergenic (+115/‑83) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,133 C→A 6.8% intergenic (+116/‑82) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
RA 3,781,138 A→C 10.0% intergenic (+121/‑77) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
JC JC 3,890,075 IS5 (–) +4 bp 100% coding (1346‑1349/1416 nt) tnaA → tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
RA 3,959,457 T→G 8.0% intergenic (+12/+75) ilvC → / ← ppiC ketol‑acid reductoisomerase, NAD(P)‑binding/peptidyl‑prolyl cis‑trans isomerase C (rotamase C)
RA 4,008,408 A→G 5.9% intergenic (+31/+31) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
RA 4,008,409 A→C 5.9% intergenic (+32/+30) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
RA 4,008,415 T→A 6.9% intergenic (+38/+24) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
RA 4,008,416 T→A 6.2% intergenic (+39/+23) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
RA 4,008,422 G→T 5.7% intergenic (+45/+17) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
RA 4,008,423 C→T 6.4% intergenic (+46/+16) rhtC → / ← rhtB threonine efflux pump/homoserine, homoserine lactone and S‑methyl‑methionine efflux pump
RA 4,070,410 C→A 6.5% intergenic (‑9/+105) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,070,413 T→G 6.8% intergenic (‑12/+102) yihR ← / ← yihS putative sulphoquinovose mutarotase/sulphoquinovose isomerase
RA 4,183,669 G→T 77.1% G809C (GGT→TGT)  rpoB → RNA polymerase, beta subunit
RA 4,185,540 C→T 14.2% P64L (CCG→CTG)  rpoC → RNA polymerase, beta prime subunit
RA 4,244,626 T→A 7.3% K150N (AAA→AAT malF ← maltose transporter subunit
RA 4,295,964 A→G 5.0% intergenic (+170/+472) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,295,965 A→C 6.2% intergenic (+171/+471) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,060 C→T 42.5% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,397 T→C 6.3% intergenic (+603/+39) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,366,854 T→G 7.5% intergenic (‑81/+37) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,366,855 C→A 7.2% intergenic (‑82/+36) dcuA ← / ← aspA C4‑dicarboxylate antiporter/aspartate ammonia‑lyase
RA 4,370,148 C→T 5.1% G89S (GGC→AGC)  yjeH ← putative transporter
RA 4,387,993 T→C 5.2% S460G (AGT→GGT)  mscM ← mechanosensitive channel protein, miniconductance
RA 4,406,129 T→C 100% intergenic (+144/‑61) purA → / → nsrR adenylosuccinate synthetase/nitric oxide‑sensitive repressor for NO regulon
RA 4,422,895 C→G 5.1% A17G (GCC→GGC)  ulaE → L‑xylulose 5‑phosphate 3‑epimerase
RA 4,457,196 A→G 5.4% intergenic (+38/+118) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,503,229 G→A 7.3% P68L (CCT→CTT)  insG ← IS4 transposase
RA 4,517,430 C→G 5.0% W96C (TGG→TGC fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,517,556 T→G 5.0% E54D (GAA→GAC) ‡ fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,517,557 T→A 5.0% E54V (GAA→GTA) ‡ fecR ← anti‑sigma transmembrane signal transducer for ferric citrate transport; periplasmic FecA‑bound ferric citrate sensor and cytoplasmic FecI ECF sigma factor activator
RA 4,614,321 A→G 8.5% intergenic (+61/+359) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,614,580 G→A 8.8% intergenic (+320/+100) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme
RA 4,621,023 G→A 8.5% L47L (CTG→CTA deoD → purine nucleoside phosphorylase 1; nicotinamide 1‑beta‑D‑riboside phosphorylase
JC JC 4,640,551 IS1 (–) +9 bp 100% intergenic (+9/‑383) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 773010 774219 1210 61 [60] [60] 61 [cydA]–[cydX] [cydA],cydB,[cydX]
* * ÷ NC_000913 1037687 1039353 1667 62 [57] [60] 61 cbdA–[cbdB] cbdA,[cbdB]
* * ÷ NC_000913 2403928 2404637 710 67 [59] [60] 61 nuoB–[nuoA] nuoB,[nuoA]
* * ÷ NC_000913 3423758–3424234 3424544–3424238 5–787 62 [55] [59] 62 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 16653NA (NA)3 (0.020) 3/264 NT NA noncoding (1267/1345 nt) IS186 repeat region
?NC_000913 = 16691 NA (NA)noncoding (1305/1345 nt) IS186 repeat region
* ? NC_000913 = 316201NA (NA)4 (0.030) 3/260 NT NA noncoding (973/1255 nt) IS3 repeat region
?NC_000913 = 316220 NA (NA)noncoding (992/1255 nt) IS3 repeat region
* ? NC_000913 1207790 =111 (0.780)39 (0.310) 23/246 NT 31.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 75 (0.600)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 1207805110 (0.770)47 (0.370) 24/246 NT 35.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 75 (0.600)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)104 (0.750) 48/270 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 2726200 =NA (NA)8 (0.060) 4/266 NT NA intergenic (‑12/+81) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon
?NC_000913 2726224 = NA (NA)intergenic (‑36/+57) rrfG/rrlG 5S ribosomal RNA of rrnG operon/23S ribosomal RNA of rrnG operon