breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 502,907 C→T G111D (GGC→GAC)  ybaL ← putative transporter YbaL
RA 664,313 A→G V293A (GTG→GCG)  rlpA ← rare lipoprotein RlpA
RA 697,968 A→G F404L (TTC→CTC)  asnB ← asparagine synthetase B
RA 1,421,650 (C)7→6 coding (532/858 nt) ydaU → Rac prophage; DUF1376 domain‑containing protein YdaU
JC JC 1,873,031 IS1 (–) +9 bp coding (991‑999/1491 nt) dgcJ → putative diguanylate cyclase DgcJ
RA 1,978,440 (G)6→7 intergenic (‑243/+78) flhD ← / ← insB‑5 DNA‑binding transcriptional dual regulator FlhD/IS1 protein InsB
RA 2,054,799 A→G Q386R (CAG→CGG)  shiA → shikimate:H(+) symporter
RA 2,293,765 T→C G380G (GGA→GGG ccmF ← holocytochrome c synthetase membrane subunit CcmF
RA 2,468,946 Δ1 bp coding (733/921 nt) yfdH → CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
RA 2,533,333 A→G T309A (ACC→GCC)  cysK → O‑acetylserine sulfhydrylase A
RA 2,718,041 G→A T163I (ACA→ATA)  yfiF ← putative methyltransferase
RA 2,730,953 T→C noncoding (205/1542 nt) rrsG ← 16S ribosomal RNA
RA 2,819,134 T→C T5A (ACT→GCT)  csrA ← carbon storage regulator
RA 3,261,353 (T)5→4 coding (1066/2295 nt) tdcE ← 2‑ketobutyrate formate‑lyase/pyruvate formate‑lyase 4
RA 3,451,917 A→G F124L (TTC→CTC)  rplD ← 50S ribosomal subunit protein L4
RA 3,584,362 A→G intergenic (+53/‑42) yrhA → / → yrhD putative uncharacterized protein YrhA/uncharacterized protein YrhD
RA 3,852,713 T→C E24E (GAA→GAG ilvB ← acetohydroxy acid synthase I subunit IlvB
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,485,023 A→G L310P (CTA→CCA)  pepA ← aminopeptidase A/I
RA 4,606,215 (G)8→9 noncoding (72/87 nt) leuP ← tRNA‑Leu

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 361189 361933 745 343 [333] [335] 341 lacA–[lacY] lacA,[lacY]
* * ÷ NC_000913 2856973 2857095 123 338 [331] [0] 586 [mutS] [mutS]
* * ÷ NC_000913 3423683–3424234 3424605–3424238 5–923 338 [332] [337] 346 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 70046 =547 (0.930)578 (1.000) 243/274 0.0 68.3% coding (3/1701 nt) araB ribulokinase
?NC_000913 2857096 = 0 (0.000)coding (4/2562 nt) mutS DNA mismatch repair protein MutS
* ? NC_000913 1207790 =91 (0.150)324 (0.620) 177/248 1.1 78.8% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 94 (0.180)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780589 (0.150)394 (0.750) 185/248 0.7 82.0% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 94 (0.180)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 1590765 =488 (0.830)1304 (2.290) 280/270 0.0 73.9% intergenic (‑229/+89) yneL/hipA putative HTH‑type transcriptional regulator YneL/serine/threonine kinase HipA
?NC_000913 = 1647024 451 (0.790)intergenic (+287/+150) flxA/ydfW Qin prophage; protein FlxA/Qin prophage; uncharacterized protein YdfW