breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 354,244 T→C S513S (AGT→AGC prpE → propionyl‑CoA synthetase
RA 1,034,793 C→T H514Y (CAC→TAC)  hyaB → hydrogenase 1 large subunit
RA 1,297,592 A→G L177P (CTG→CCG)  adhE ← aldehyde‑alcohol dehydrogenase
RA 1,370,044 (T)9→8 intergenic (+41/‑172) pspE → / → ycjM thiosulfate sulfurtransferase PspE/glucosylglycerate phosphorylase
JC JC 1,873,031 IS1 (–) +9 bp coding (991‑999/1491 nt) dgcJ → putative diguanylate cyclase DgcJ
RA 1,937,940 G→A A98T (GCC→ACC)  pykA → pyruvate kinase II
RA 1,979,176 T→C T14A (ACT→GCT)  insA‑5 ← IS1 protein InsA
RA 2,213,249 A→G F466L (TTT→CTT)  btsS ← high‑affinity pyruvate receptor
RA 3,584,362 A→G intergenic (+53/‑42) yrhA → / → yrhD putative uncharacterized protein YrhA/uncharacterized protein YrhD
RA 3,638,367 T→C I242I (ATT→ATC pitA → metal phosphate:H(+) symporter PitA
RA 3,952,835 A→G T118A (ACC→GCC)  ilvE → branched‑chain‑amino‑acid aminotransferase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,816 A→G intergenic (‑75/+583) yjdN ← / ← yjdM conserved protein YjdN/conserved protein YjdM
RA 4,361,448 (A)8→7 coding (487/1539 nt) cadC ← DNA‑binding transcriptional activator CadC
RA 4,549,187 C→T A127V (GCG→GTG)  fimH → type 1 fimbriae D‑mannose specific adhesin

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 361136 361966 831 1697 [1665] [1665] 1677 [cynX]–[lacY] [cynX],lacA,[lacY]
* * ÷ NC_000913 2856930 2857095 166 1685 [1665] [0] 2100 [mutS] [mutS]
* * ÷ NC_000913 3423453–3423551 3424627–3424238 688–1175 1677 [1668] [1664] 1686 [rrfF]–[rrlD] [rrfF],thrV,rrfD,[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 70046 =2067 (1.000)2049 (1.010) 290/276 0.0 67.0% coding (3/1701 nt) araB ribulokinase
?NC_000913 2857096 = 0 (0.000)coding (4/2562 nt) mutS DNA mismatch repair protein MutS
* ? NC_000913 1207790 =352 (0.170)1554 (0.850) 261/250 0.0 82.3% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 357 (0.200)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 1207805352 (0.170)1512 (0.830) 262/250 0.0 81.9% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 357 (0.200)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 4542682 =1009 (0.490)1308 (0.680) 264/264 -0.0 56.4% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 1077 (0.560)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 45426901012 (0.490)1251 (0.650) 266/264 -0.0 55.3% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 1077 (0.560)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit