breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 321,564 (G)7→6 intergenic (+483/‑44) rclR → / → ykgE DNA‑binding transcriptional activator RclR/putative lactate utilization oxidoreductase YkgE
RA 661,135 C→T G82S (GGT→AGT)  ybeF ← putative LysR‑type DNA‑binding transcriptional regulator YbeF
RA 1,097,037 (G)7→8 intergenic (+208/+528) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
RA 1,183,252 A→G G123G (GGT→GGC potC ← spermidine preferential ABC transporter membrane subunit PotC
RA 1,351,196 (G)7→6 intergenic (‑157/+211) fabI ← / ← ycjD enoyl‑[acyl‑carrier‑protein] reductase/DUF559 domain‑containing protein YcjD
RA 1,554,784 (T)7→6 coding (886/1698 nt) maeA ← malate dehydrogenase, NAD‑requiring
RA 1,617,887 G→A C287Y (TGC→TAC)  ydeA → L‑arabinose exporter
RA 1,714,326 (T)9→8 intergenic (+55/‑51) dtpA → / → gstA dipeptide/tripeptide:H(+) symporter DtpA/glutathione S‑transferase GstA
RA 1,818,550 (C)7→8 intergenic (‑50/+55) chbF ← / ← chbR monoacetylchitobiose‑6‑phosphate hydrolase/DNA‑binding transcriptional dual regulator ChbR
JC JC 1,873,031 IS1 (–) +9 bp coding (991‑999/1491 nt) dgcJ → putative diguanylate cyclase DgcJ
RA 2,071,056 T→C pseudogene (271/426 nt) yeeP → CP4‑44 prophage; putative GTP‑binding protein
RA 2,074,362 (C)6→5 coding (2824/3120 nt) flu → CP4‑44 prophage; self recognizing antigen 43 (Ag43) autotransporter
RA 2,771,218 T→C V24A (GTC→GCC)  yfjT → CP4‑57 prophage; uncharacterized protein YfjT
RA 3,035,546 T→C T246A (ACG→GCG)  prfB ← peptide chain release factor RF2
RA 3,279,072 A→G V53V (GTA→GTG kbaZ → tagatose‑1,6‑bisphosphate aldolase 1 subunit KbaZ
RA 3,302,617 G→A R43R (CGG→CGA yhbV → putative peptidase YhbV
RA 3,385,853 (T)8→9 intergenic (+52/+4) yhcN → / ← yhcO DUF1471 domain‑containing stress‑induced protein YhcN/putative barnase inhibitor
RA 3,421,855 (G)5→6 coding (531/1104 nt) yhdY → putative ABC transporter membrane subunit YhdY
RA 3,465,686 (A)8→7 coding (144/678 nt) gspO → Type II secretion system prepilin peptidase
RA 3,488,684 T→C Y632H (TAT→CAT)  yhfK → putative transporter YhfK
RA 3,584,362 A→G intergenic (+53/‑42) yrhA → / → yrhD putative uncharacterized protein YrhA/uncharacterized protein YrhD
RA 3,931,561 (G)7→8 coding (246/1869 nt) kup → K(+):H(+) symporter Kup
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,399,887 A→G Q212Q (CAA→CAG miaA → tRNA dimethylallyltransferase
RA 4,545,070 C→T intergenic (+41/‑26) fimC → / → fimD type 1 fimbriae periplasmic chaperone/type I fimbriae usher protein
RA 4,549,342 A→G T179A (ACT→GCT)  fimH → type 1 fimbriae D‑mannose specific adhesin
RA 4,556,769 (T)9→8 coding (116/144 nt) ytiD → protein YtiD
RA 4,638,793 A→G T206A (ACC→GCC)  creD → putative inner membrane protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 361171 361944 774 352 [345] [350] 355 lacA–[lacY] lacA,[lacY]
* * ÷ NC_000913 2856966 2857095 130 352 [351] [0] 557 [mutS] [mutS]
* * ÷ NC_000913 3423681–3424607 3424607 1–927 353 [351] [348] 353 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 70046 =533 (0.960)543 (1.000) 243/274 0.0 67.6% coding (3/1701 nt) araB ribulokinase
?NC_000913 2857096 = 0 (0.000)coding (4/2562 nt) mutS DNA mismatch repair protein MutS
* ? NC_000913 1207790 =158 (0.280)279 (0.570) 162/248 2.3 65.6% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 153 (0.310)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 1207805158 (0.280)318 (0.650) 191/248 0.4 68.5% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 153 (0.310)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment