| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 151,656 | 0 | T | G | 33.3% | 66.8 / 36.7 | 48 | A192A (GCA→GCC) | yadL | putative fimbrial protein YadL |
| Reads supporting (aligned to +/- strand): ref base T (0/32); new base G (16/0); total (16/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-13 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCA > NC_000913/151591‑151723 | gggATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGc > 1:1724763/1‑68 (MQ=255) aTGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGgaga > 1:893038/1‑68 (MQ=255) aTGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGgaga > 1:53482/1‑68 (MQ=255) aTGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGgaga > 1:3477099/1‑68 (MQ=255) tGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCCGaa > 1:213746/1‑68 (MQ=255) gCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaa < 1:3505531/68‑1 (MQ=255) gCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGaaa < 1:2913795/68‑1 (MQ=255) gCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGACTGGTGCAGaaa < 1:2555472/68‑1 (MQ=255) tatataGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCCGAAAAca > 1:3155310/1‑67 (MQ=255) taGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTAcc < 1:1622900/68‑1 (MQ=255) taGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAGAAAACTTAcc > 1:3979340/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGCGAAAACTTAccc > 1:3282807/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAGGAAAATTAacc > 1:3117425/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAGAAAACTTAccc > 1:1612085/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAGAAAAATTAccc > 1:302416/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCGGAAAAATTAccc > 1:1667694/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAGAAAACTTAccc > 1:760629/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAGAAAAATTAccc > 1:2097935/1‑68 (MQ=255) aGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAGAAAAATTAccc > 1:1046741/1‑68 (MQ=255) tCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAGAAAAATTAcccccc > 1:1092262/1‑65 (MQ=255) cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGt < 1:2223429/68‑1 (MQ=255) cTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGt < 1:2612414/68‑1 (MQ=255) gAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGa < 1:1869894/68‑1 (MQ=255) aaTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGAc < 1:1246440/68‑1 (MQ=255) aaTCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGAc < 1:265181/68‑1 (MQ=255) tCTGTAAGTCTGTTAGTTNTAGGTTACTGTAAATGTGGCTGGGGCAGGAAAAATACCCCCCGGGACAt > 1:3091325/1‑68 (MQ=255) tCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACAt < 1:2871142/68‑1 (MQ=255) tGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTg < 1:432960/68‑1 (MQ=255) gTAAGTCTGTTAGTTGTAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGt < 1:800878/68‑1 (MQ=255) gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGt < 1:1277726/68‑1 (MQ=255) gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGt < 1:2491843/68‑1 (MQ=255) gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGt < 1:840997/68‑1 (MQ=255) gTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGt < 1:2947900/68‑1 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAGAAAACTTACCCGCAGTGACATTGtttt > 1:178886/1‑67 (MQ=255) aGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGCAGAAAAATTACCCGCAGTGACAATGtttt > 1:961205/1‑67 (MQ=255) tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGGGAAAAATTAACCGCAGGGGCATATTTTatg > 1:3386085/1‑66 (MQ=255) tCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGGGGAGAAAAATTACCCCCAGGGACATTGtttttg > 1:3310324/1‑65 (MQ=255) tGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACAGt < 1:703338/68‑1 (MQ=255) tagttaTAGGTTACTGTAAATGTGGCTGGGGCAGAAAACTTACCCCCCGGGGCATTGTTTTCGGgttt > 1:2871607/1‑68 (MQ=255) taGGTTACTGTAAATGTGGCTGGGGCAGAAAAAATACGCGCCGGGACATTGTTTATGGGTtttttttt > 1:2178569/1‑68 (MQ=255) ggTTACTGTAAATGTGGCTGGGGGGGAAAACTTACCCGCCGTGGCATTGTTTTCGGGTtttttttttt > 1:3514455/1‑68 (MQ=255) ggTTACTGTAAATGTGGCTGGGGGAGGAAAATTACCCGCAGGGGCCTTTTTTTTGGGTtttttttttt > 1:519480/1‑68 (MQ=255) ggTTACTGTAAATGTGGCTGGGGCGGGAAAATTACCCCCCGGGGCCTTTTTTTCGGGTtttttttttt > 1:868300/1‑68 (MQ=255) ggTTACTGTAAATGTGGCTGGGGCAGAAAACTTACCCGCCGGGGCATTGTTTACGGTTTTAttttttt > 1:2373977/1‑68 (MQ=255) ggTTACTGTAAATGTGGCTGGGGCAGAAAACTTACCCCCCGGGGCATTGTTTTCGGtttttttttttt > 1:1886851/1‑68 (MQ=255) ggTTACTGTAAATGTGGCTGGGGCAGAAAAATTACCCCCAGCGGCATTTTTTTCGGGTtttttttttt > 1:298456/1‑68 (MQ=255) aCTGTAAATGTGGCTGGGGGAGAAAACTTACCCCCCGGGACATTGTTTTCGGGTTTTTTTTTTTTCAc > 1:3247393/1‑68 (MQ=255) aCTGTAAATGTGGCTGGGGGAGAAAACTTACCCCCAGGGGGATTGTTTTCGGTTTTATTTTTTTTCAc > 1:3855343/1‑68 (MQ=255) aCTGTAAATGTGGCTGGGGCAGAAAAATTACCCCCCGTGACATTGTTTTCGGGTTTTTTTTTTTTCAc > 1:3402741/1‑68 (MQ=255) tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:771838/68‑1 (MQ=255) tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:677104/68‑1 (MQ=255) tGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGa < 1:2859333/68‑1 (MQ=255) tGTAAATGTGGCTGGGGCAGAAAACTTACCCGCAGTGAAATTGTTTACGGTTTTTTTTTTTTTCACGa > 1:1291788/1‑68 (MQ=255) tGTAAATGTGGCTGGGGCAGAAAACTTACCCGCAGGGGCATTGTTTTCGGGTTTTTTTTTTTTCACGa > 1:2099172/1‑68 (MQ=255) gTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGAc < 1:3555486/68‑1 (MQ=255) tAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGAcc < 1:3237761/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAACCTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:1376378/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGTCCAgg < 1:2409764/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:1888501/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3729832/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:3160377/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:867774/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2573792/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:640814/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:1732225/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAgg < 1:2607774/68‑1 (MQ=255) aTGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTGTCCACGGCCAgg < 1:2543336/68‑1 (MQ=255) tgtgGCTGGGGCAGAAAACTTACCCGCAGGGACATTGTTTTTGGGTTTTTTTTTTTTCACGACCAggg > 1:1924792/1‑67 (MQ=255) ggTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACt < 1:1820552/68‑1 (MQ=255) tGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa < 1:1936965/68‑1 (MQ=255) tGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCa < 1:3441196/68‑1 (MQ=255) | GGGATGCATATATAGTTCCTGAATCTGTAAGTCTGTTAGTTATAGGTTACTGTAAATGTGGCTGGTGCAGAAAACTTACCCGCAGTGACATTGTTTACGGTTTTATTTTTTTCCACGACCAGGCGTGCACTCA > NC_000913/151591‑151723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |