| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 424,518 | 0 | A | C | 33.3% | 37.7 / 13.4 | 27 | V134G (GTG→GGG) | acpH | acyl carrier protein phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base A (18/0); new base C (0/9); total (18/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.13e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAAT > NC_000913/424451‑424585 | cAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCa > 1:544119/1‑68 (MQ=255)cAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCa > 1:2050180/1‑68 (MQ=255) gACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCAcc > 1:1413631/1‑68 (MQ=255) cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCac > 1:3780700/1‑67 (MQ=255) cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAg > 1:905286/1‑68 (MQ=255) cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAg > 1:2177206/1‑68 (MQ=255) cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAg > 1:207050/1‑68 (MQ=255) cGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAg > 1:1563578/1‑68 (MQ=255) gcggcggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACt > 1:2156996/1‑68 (MQ=255) gcggcggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACt > 1:839762/1‑68 (MQ=255) gcggcggcTTGCCATGCCGTTTAACACGTTCTGGATGAAATACATATCGCGATAGCGCACCAGCCACt > 1:2678483/1‑68 (MQ=255) cTTGCGGTGCGGTTTAACGCGTTCTGGATAAAATCCATATCGGGATAGCCCCCCAGCCACTGCTCTGa < 1:523196/68‑1 (MQ=255) cTTGCCATGCCTTTTAACCCGTTCTGGATAAAACCCATATCGCGATACCGCCCCAGCCACTGCTCTGa < 1:3160583/68‑1 (MQ=255) cTTGCCATGCCGTTTAACCCGTTCTGGATGAAATCCATATCGCGATAGCGCCCCAGCCACTGCTCTGa < 1:1628058/68‑1 (MQ=255) cTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCCCCAGCCACTGCTCTGa < 1:699678/68‑1 (MQ=255) cTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCCCCCCAGCCACTGCTCTGa < 1:3007997/68‑1 (MQ=255) cTTGCCATGCCGTTTAACACGTTCTGGATAAAATCCATACCGCGATAGCGCCCCAGCCACTGCTCTGa < 1:1240997/68‑1 (MQ=255) atcccGTTTAACCCGTTCTGGATGAAACCCATATCGCGATAGCGCCCCAGCCACTGCTCTGACCACaa < 1:2622897/65‑1 (MQ=255) ccGTTTAACACGTTCTGGATAAAACCCATACCGCGATGGCGCCCCAGCCACTGCTCTGACCACAAGTa < 1:1714570/68‑1 (MQ=255) cGTTTAACACGTTCTGGATGAAATCCACATCGCGCTAGCGCACCAGCCACTGCTCTGCCCACAAGTaa > 1:2853685/1‑68 (MQ=255) tAACAGTTTCTGGATGAAATCCATTCCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGt < 1:1895424/68‑1 (MQ=255) tAACACGTTCTGGATGAAATCCATATCGCGATAGCGCCCCAGCCACTGCTCTGACCACAAGTAATTGt < 1:2669800/68‑1 (MQ=255) acacGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTc > 1:2240436/1‑68 (MQ=255) acGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAg > 1:3762478/1‑68 (MQ=255) cGTTCTGGATGAAGTCCATCTCGCGATAGCGCCCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGa < 1:11211/68‑1 (MQ=255) tCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTg > 1:2911962/1‑68 (MQ=255) tCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTg > 1:3026253/1‑68 (MQ=255) cTGGATGAAATCCATATCGCGATAGCGCCCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa < 1:826198/68‑1 (MQ=255) agggATGAAATCCATATCGCGATAGCGCCCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa < 1:3841953/66‑1 (MQ=255) agggATAAAAGCCATATCGCGATAGCCCCCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGa < 1:1001491/66‑1 (MQ=255) gATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATaa > 1:3573675/1‑68 (MQ=255) gATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATaa > 1:1222352/1‑68 (MQ=255) taaaaTCCATATCGCGATAGCGCCCCAGCCACTGTTCTGACCACAAGTAATTGTTCAGATTGATaaaa < 1:2677449/66‑1 (MQ=255) tCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTgg > 1:2121667/1‑68 (MQ=255) gcgATACCGCCCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGt < 1:50139/68‑1 (MQ=255) aGCGCACCAGCCACTGCTCTGACCACCAGTAATTGTTCAGATTGATAAACCGCGGCGGTGAGTCCGGc > 1:2890411/1‑68 (MQ=255) aGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGc > 1:2288687/1‑68 (MQ=255) gcACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCaaa > 1:3478082/1‑68 (MQ=255) gcACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCaaa > 1:1247337/1‑68 (MQ=255) aCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGCTTGATAAAACGTGGCGGTGAGTCCGGCAAAAt > 1:3191135/1‑68 (MQ=255) aCCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAAt > 1:2079918/1‑68 (MQ=255) | CAGACGTGGGCGGCGGCTTGCCATGCCGTTTAACACGTTCTGGATGAAATCCATATCGCGATAGCGCACCAGCCACTGCTCTGACCACAAGTAATTGTTCAGATTGATAAAACGTGGCGGTGAGTCCGGCAAAAT > NC_000913/424451‑424585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |