| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,280,704 | 0 | A | C | 43.8% | 11.5 / 11.7 | 16 | T25P (ACC→CCC) | radD | putative DNA repair helicase RadD |
| Reads supporting (aligned to +/- strand): ref base A (6/3); new base C (0/7); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACG > NC_000913/2280638‑2280768 | tttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAc < 1:537521/68‑1 (MQ=255)tttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAc < 1:313703/68‑1 (MQ=255) ttACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAAcc < 1:3387718/68‑1 (MQ=255) tatCAGAAAGAAGCCGTGGATGCCACGCTAAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGt < 1:420981/68‑1 (MQ=255) cAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGtgct < 1:3477027/68‑1 (MQ=255) gCAAAAAGCCGTGGATCCCCCGCCCACCCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGtgctgc < 1:844214/68‑1 (MQ=255) acAAAAAGCGGGGGATCCCACGCTCAACCTTTTC‑CGTCGTCAAAAACCCCCTGCCGTTATCGtgctgc < 1:980891/67‑1 (MQ=255) aaaagaagCCGGGGGTGCCACGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGtgctgc < 1:1914180/66‑1 (MQ=255) aagaagCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGccc > 1:785822/1‑68 (MQ=255) gCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCgg > 1:1292238/1‑68 (MQ=255) aTGCCACTCACAACCATTTC‑CGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt < 1:3404766/68‑1 (MQ=255) aTGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGt > 1:3000740/1‑68 (MQ=255) ccACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTaaa > 1:1649780/1‑68 (MQ=255) ccccTCAACCGTTTT‑CGTCGTCAAAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg < 1:994262/65‑1 (MQ=255) ccGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg < 1:2513257/67‑1 (MQ=255) ccGCTC‑ACCATTTTCCGTCTTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAg < 1:2234800/67‑1 (MQ=255) cGCTCAACCATTTT‑CGTCGTCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGc < 1:535641/68‑1 (MQ=255) cagccATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCNGCGCAGCTAAAAGCCTgg < 1:3088261/65‑1 (MQ=255) ccATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGa > 1:2829413/1‑68 (MQ=255) ttt‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGCAAAAGCCTGGTGATAGc > 1:3143992/1‑68 (MQ=255) cttcgtcATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa < 1:3554436/66‑1 (MQ=255) cgtcgtCATAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa < 1:3122049/68‑1 (MQ=255) cgtcatCAGAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGa < 1:2504026/68‑1 (MQ=255) tcAGAAACCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTgg < 1:2979510/68‑1 (MQ=255) aaacCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg < 1:3807490/68‑1 (MQ=255) aaacCCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACg < 1:582611/68‑1 (MQ=255) | TTTACACTTCGCCCATATCAGCAAGAAGCCGTGGATGCCACGCTCAACCATTTT‑CGTCGTCATAAAACCCCTGCCGTTATCGTGCTGCCCACCGGCGCAGGTAAAAGCCTGGTGATAGCGGAACTGGCACG > NC_000913/2280638‑2280768 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |