| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,710,279 | 0 | A | C | 30.8% | 61.2 / 27.2 | 39 | intergenic (‑115/‑141) | rseD/nadB | rpoE leader peptide/L‑aspartate oxidase |
| Reads supporting (aligned to +/- strand): ref base A (27/0); new base C (0/12); total (27/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.56e-10 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTA > NC_000913/2710214‑2710346 | gAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTccacc > 1:2412413/1‑68 (MQ=255) aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTccaccct > 1:1281049/1‑66 (MQ=255) aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccag > 1:1595095/1‑68 (MQ=255) aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccag > 1:1849174/1‑68 (MQ=255) aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccag > 1:3934739/1‑68 (MQ=255) aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccag > 1:2120301/1‑68 (MQ=255) aaTGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCAccag > 1:2058262/1‑68 (MQ=255) tGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCTTTTTTATTTCACCACCTAATCCTCCAccagcc > 1:25715/1‑68 (MQ=255) ttAACTGCCTGAAGAGTACCCCAGCGGCCTTTTTATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACt < 1:1033878/68‑1 (MQ=255) aCTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACNTAATCCTCCACTAGCCAGTAACTtct > 1:3257127/1‑68 (MQ=255) aCTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTtct > 1:2886310/1‑68 (MQ=255) aCTGCCTGAAGAGAAACCCACCGGCTTTTTTATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTtct < 1:3520121/68‑1 (MQ=255) cTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTtctc > 1:1228036/1‑68 (MQ=255) cTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTtctc > 1:249811/1‑68 (MQ=255) gagTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCAAGTAACTTCTCTTTTTCTcg > 1:3324935/1‑68 (MQ=255) gagCAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcg > 1:3607732/1‑68 (MQ=255) agTAACCCAGCGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc > 1:2576823/1‑68 (MQ=255) agTAACCCACCGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTtctccc > 1:474534/1‑66 (MQ=255) agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc > 1:3061393/1‑68 (MQ=255) agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc > 1:2671031/1‑68 (MQ=255) agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc > 1:2529833/1‑68 (MQ=255) agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc > 1:4061691/1‑68 (MQ=255) agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc > 1:3952287/1‑68 (MQ=255) agTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCACCCAGTAACTTCTCTTTTTCTcgc > 1:3032542/1‑68 (MQ=255) gTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgcc > 1:2232818/1‑68 (MQ=255) accccATCGGCCTTTTTTTTCCCCCCCCTACCCCCCCCCCAGCCAGTAACTTCTCTTTTTCTcgccgc < 1:389164/66‑1 (MQ=255) accccAACGGCTTTTTTTTTCCCCCCCCAAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTcgccgc < 1:3433976/66‑1 (MQ=255) aaCCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgccgc > 1:2027520/1‑68 (MQ=255) ccccACCGGCTTTTTTATTCCCCCACCAAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCgccgcc < 1:2383723/67‑1 (MQ=255) ccccACCGCCTTTTTTATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCgccgcc < 1:3500074/67‑1 (MQ=255) ccccACCGCCCTTTTTATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCgccgcc < 1:1140437/67‑1 (MQ=255) ccccAACGGCTTTTTTTTTCCCCCCCATAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCgccgcc < 1:3967633/67‑1 (MQ=255) ccACCGGCCTTTTTATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCt < 1:2075809/68‑1 (MQ=255) ccAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCt > 1:561817/1‑68 (MQ=255) ccAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCt > 1:3644615/1‑68 (MQ=255) aaCGACTTTTTTATTACCCCCCCTACCCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGc < 1:2186798/68‑1 (MQ=255) aaCCGCCTTTTTACTTCACCACCTACCCCCCCCCCAGCCAGTAACTTCTNTTTTTCTCGCCGCCCTGc < 1:3099577/68‑1 (MQ=255) cGGCTTTTTTTTTCCCCCACCTAACCCCCCCCCAGCCAGTAAGTTCTCTTTTTCTCGCCGCCCTGCGt < 1:2743552/68‑1 (MQ=255) ttttATTCCCCCCCCTACCCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt < 1:1959891/68‑1 (MQ=255) ttttATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt < 1:493954/68‑1 (MQ=255) ttttATTCCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt < 1:2243208/68‑1 (MQ=255) tttaATTTCCCCCCCAAATCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgt < 1:3688861/68‑1 (MQ=255) tttATTTCCCCCCCTAACCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCgtg < 1:295385/68‑1 (MQ=255) caccTACCCCCCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACt < 1:1157508/68‑1 (MQ=255) cctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCCGCGTGTTTAGCAACTGTAACaaa > 1:3219024/1‑68 (MQ=255) cctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACaaa > 1:74083/1‑68 (MQ=255) cctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACaaa > 1:3337907/1‑68 (MQ=255) cctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACaaa > 1:3120602/1‑68 (MQ=255) ctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAat > 1:4194666/1‑68 (MQ=255) ctccACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAat > 1:538426/1‑68 (MQ=255) accaGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTa > 1:2550286/1‑68 (MQ=255) accaGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTa > 1:3368519/1‑68 (MQ=255) accaGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTa > 1:3345103/1‑68 (MQ=255) | GAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTA > NC_000913/2710214‑2710346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |