| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,563,481 | 0 | T | G | 29.0% | 59.7 / 17.1 | 31 | V490G (GTG→GGG) | glpD | aerobic glycerol 3‑phosphate dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/22); new base G (8/1); total (8/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.14e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAT > NC_000913/3563415‑3563544 | ggCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggtg > 1:834898/1‑68 (MQ=255) cGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGa < 1:451783/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCtggggggg > 1:777679/1‑66 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:3845294/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:1720260/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:2957972/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:2071567/68‑1 (MQ=255) gTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAg < 1:2357675/68‑1 (MQ=255) tCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGt < 1:206588/68‑1 (MQ=255) gCACAAAACAAGGCATGTGGCTGAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:257829/68‑1 (MQ=255) gCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGtat < 1:3603566/68‑1 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATAc < 1:3721907/68‑1 (MQ=255) acaAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGCGGGTATAc > 1:3653408/1‑68 (MQ=255) aaaCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGAGTATACgca < 1:17498/68‑1 (MQ=255) aCAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAagcagc > 1:2157886/1‑68 (MQ=255) ggaTGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAgg < 1:2558355/66‑1 (MQ=255) gCATTTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAgg < 1:3532862/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAgg < 1:2859480/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAgg < 1:2869813/68‑1 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATAAGCAGACGAgg > 1:1014946/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGGTACGGAGCcgaag > 1:859005/1‑66 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGGAgggg > 1:84252/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGGAGAgg > 1:3469130/1‑68 (MQ=255) gCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATAAGCAGCAgggg > 1:2277983/1‑68 (MQ=255) cATCTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGt < 1:2223326/68‑1 (MQ=255) tgGCTAAATGCGGATCAAGAGTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATc < 1:1494435/68‑1 (MQ=255) aTGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGcg < 1:494194/68‑1 (MQ=255) tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGTATACGCAGCAGAGGGTATCGCTTGcgg > 1:2110792/1‑67 (MQ=255) tGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGAATAGGGAGCAGAAGGGATCGCTggggg > 1:3888679/1‑65 (MQ=255) gCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGAGGGTATTGCTGGggtc > 1:3940607/1‑68 (MQ=255) aTCAACAATCTCGTGTGAGTCAGTGGCTTGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTaa < 1:3681471/68‑1 (MQ=255) caaTCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTaa < 1:2097417/68‑1 (MQ=255) ctcGTGTGAGTCAGTGGCTGGGGGGGGATACGGATGAGCGGTGTTTCCTGGGGGCGTCAATTAACggg > 1:4152877/1‑66 (MQ=255) cGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGCGGGTATTGCTGGCGTCGGGAATTAACGTaaa > 1:2266662/1‑67 (MQ=255) cGTGTGAGTCAGTGGCTGGGGGGGGATACGCAGCAGACGGTTTTGCTGGCGTCGGTAATTAAAGTAAg > 1:280217/1‑68 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGt < 1:1111130/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGt < 1:4006617/68‑1 (MQ=255) tgtgAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGt < 1:3534502/68‑1 (MQ=255) taGTGTCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAgg < 1:469815/67‑1 (MQ=255) gCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa < 1:4140651/68‑1 (MQ=255) gCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGa < 1:1207930/68‑1 (MQ=255) cTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAt < 1:3457317/68‑1 (MQ=255) | GGCGTCGCACAAAACAAGGCATGTGGCTAAATGCGGATCAACAATCTCGTGTGAGTCAGTGGCTGGTGGAGTATACGCAGCAGAGGTTATCGCTGGCGTCGTAAATTAACGTAAGGTGATCAGGTCAGAT > NC_000913/3563415‑3563544 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |