breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 3,336 G→T 24.4% G179V (GGG→GTG)  thrB → homoserine kinase
RA 34,502 G→T 16.2% G68V (GGG→GTG)  caiF → cai operon transcriptional activator
RA 63,846 G→T 12.5% A645A (GCC→GCA polB ← DNA polymerase II
RA 72,406 G→T 12.1% L461M (CTG→ATG)  sgrR ← transcriptional DNA‑binding transcriptional activator of sgrS sRNA
RA 92,346 G→T 24.0% G404C (GGC→TGC)  murF → UDP‑N‑acetylmuramoyl‑tripeptide:D‑alanyl‑D‑ alanine ligase
RA 100,246 C→T 11.8% T202M (ACG→ATG)  ftsQ → divisome assembly protein, membrane anchored protein involved in growth of wall at septum
RA 102,375 G→T 18.3% G195V (GGT→GTT)  ftsZ → GTP‑binding tubulin‑like cell division protein
RA 108,846 G→C 36.4% P81A (CCT→GCT)  zapD ← FtsZ stabilizer
RA 164,694 G→C 28.6% E242Q (GAA→CAA)  fhuA → ferrichrome outer membrane transporter
RA 164,697 Δ1 bp 28.6% coding (727/2244 nt) fhuA → ferrichrome outer membrane transporter
RA 164,698 A→T 28.6% E243V (GAG→GTG)  fhuA → ferrichrome outer membrane transporter
RA 169,317 G→T 8.7% G457C (GGC→TGC)  fhuB → fused iron‑hydroxamate transporter subunits of ABC superfamily: membrane components
RA 186,083 G→T 14.3% intergenic (‑90/‑116) map ← / → tff methionine aminopeptidase/novel sRNA, function unknown
RA 205,422 C→T 11.8% I105I (ATC→ATT accA → acetyl‑CoA carboxylase, carboxytransferase, alpha subunit
RA 217,981 G→T 15.3% P43Q (CCG→CAG)  metI ← DL‑methionine transporter subunit
RA 221,373:1 +G 15.9% noncoding (1116/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,380 T→A 15.9% noncoding (1123/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,390 Δ1 bp 15.9% noncoding (1133/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,391 G→A 15.9% noncoding (1134/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,393 C→T 16.7% noncoding (1136/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,395 G→A 16.7% noncoding (1138/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,397 C→T 16.7% noncoding (1140/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,398 C→T 16.7% noncoding (1141/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,402 A→C 16.6% noncoding (1145/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,407 A→T 16.7% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 16.4% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 17.9% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 21.2% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 21.2% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,455 G→A 30.0% noncoding (1198/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 263,966 G→T 12.2% R236R (CGG→AGG)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 274,938 T→A 18.2% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 309,925 Δ1 bp 26.7% intergenic (‑409/‑143) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
RA 309,926 Δ1 bp 26.7% intergenic (‑410/‑142) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
RA 309,927 Δ1 bp 26.7% intergenic (‑411/‑141) ykgP ← / → eaeH pseudogene, oxidoreductase family/pseudogene, attaching and effacing protein homology;factor; Not classified
RA 383,954 A→C 16.5% Q235P (CAG→CCG)  tauD → taurine dioxygenase, 2‑oxoglutarate‑dependent
RA 398,423 C→A 18.2% G407G (GGC→GGA phoA → bacterial alkaline phosphatase
RA 470,183 C→T 14.3% P145P (CCG→CCA tesB ← acyl‑CoA thioesterase 2
RA 497,341 G→T 7.4% R452S (CGT→AGT)  ybaL ← inner membrane putative NAD(P)‑binding transporter
RA 498,188 G→T 12.9% P169P (CCC→CCA ybaL ← inner membrane putative NAD(P)‑binding transporter
RA 504,115 G→T 15.5% intergenic (+100/+216) ybaQ → / ← copA putative DNA‑binding transcriptional regulator/copper transporter
RA 504,928 C→G 15.4% A636A (GCG→GCC copA ← copper transporter
RA 504,929:1 +C 15.4% coding (1907/2505 nt) copA ← copper transporter
RA 525,408 A→T 11.8% pseudogene (65/357 nt) ylbG ← pseudogene, DNA‑binding transcriptional regulator homology
RA 545,098 C→A 40.5% P6T (CCT→ACT)  ylbF → putative anaerobic allantoin catabolic oxamate carbamoyltransferase; DUF2877 family protein
RA 621,365 G→T 16.0% W342L (TGG→TTG)  entC → isochorismate synthase 1
RA 641,105 G→T 14.5% T311N (ACT→AAT)  citT ← citrate:succinate antiporter
RA 709,367 C→A 27.3% T118T (ACC→ACA pgm → phosphoglucomutase
RA 717,291 C→A 21.2% G860G (GGG→GGT kdpD ← fused sensory histidine kinase in two‑component regulatory system with KdpE: signal sensing protein
RA 725,992 C→A 6.7% P318P (CCC→CCA rhsC → Rhs family putative polymorphic toxin
RA 726,100 A→G 10.7% R354R (CGA→CGG rhsC → Rhs family putative polymorphic toxin
RA 726,110 T→C 6.2% C358R (TGT→CGT)  rhsC → Rhs family putative polymorphic toxin
RA 741,049 G→A 10.8% E143E (GAG→GAA ybgL → UPF0271 family protein
RA 764,985 C→A 18.4% R518R (CGG→AGG)  mngB → alpha‑mannosidase
RA 774,198 C→A 11.8% Q335K (CAG→AAG)  tolB → periplasmic protein
RA 817,379 G→T 13.8% I192I (ATC→ATA ybhN ← UPF0104 family inner membrane protein
RA 821,941 C→T 11.1% P256P (CCG→CCA ybhS ← inner membrane putative ABC superfamily transporter permease
RA 921,462 C→T 22.2% intergenic (‑35/+219) serW ← / ← infA tRNA‑Ser/translation initiation factor IF‑1
RA 989,052 C→A 16.7% R150L (CGA→CTA)  ssuB ← alkanesulfonate transporter subunit
RA 989,067 C→A 16.7% R145I (AGA→ATA)  ssuB ← alkanesulfonate transporter subunit
RA 1,016,418 C→A 100% intergenic (‑43/‑176) sulA ← / → sxy SOS cell division inhibitor/CRP‑S‑dependent promoter expression factor
RA 1,050,927 G→T 12.1% L236L (CTC→CTA torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,052,041 G→T 14.3% G109C (GGT→TGT)  torT → periplasmic sensory protein associated with the TorRS two‑component regulatory system
RA 1,108,650 C→A 14.4% R778S (CGT→AGT)  opgH → membrane glycosyltransferase; nutrient‑dependent cell size regulator, FtsZ assembly antagonist
RA 1,158,148 G→T 19.5% G19W (GGG→TGG)  lpoB → OM lipoprotein stimulator of MrcB transpeptidase
RA 1,282,287 T→A 17.5% intergenic (+305/+235) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,288,276 C→A 14.0% V35F (GTT→TTT)  hns ← global DNA‑binding transcriptional dual regulator H‑NS
RA 1,310,303 C→A 22.2% intergenic (‑11/+370) yciG ← / ← trpA KGG family protein/tryptophan synthase, alpha subunit
RA 1,378,274 C→A 14.9% intergenic (‑56/‑100) ycjW ← / → ycjX LacI family putative transcriptional repressor/DUF463 family protein, putative P‑loop NTPase
RA 1,392,255 G→A 28.5% L209L (CTG→TTG)  uspE ← stress‑induced protein
RA 1,415,096 G→T 17.5% Q52H (CAG→CAT ydaT → Rac prophage; uncharacterized protein
RA 1,530,041 G→T 28.8% F25L (TTC→TTA yddE ← PhzC‑PhzF family protein
RA 1,531,796 G→T 9.6% Q439K (CAG→AAG)  narY ← nitrate reductase 2 (NRZ), beta subunit
RA 1,651,091 C→A 15.5% V208V (GTG→GTT ynfC ← UPF0257 family lipoprotein
RA 1,661,618 G→T 14.8% R402S (CGT→AGT)  mlc ← glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
RA 1,665,109 G→T 22.2% W385L (TGG→TTG)  ynfM → putative arabinose efflux transporter
RA 1,678,059 C→A 23.5% L307L (CTC→CTA rstB → sensory histidine kinase of RstAB two‑component system, low Mg‑responsive via PhoQP
RA 1,721,888 C→A 7.4% R325S (CGT→AGT)  nemA → chromate reductase, quinone reductase, FMN‑linked; N‑Ethylmaleimide reductase; old yellow enzyme
RA 1,759,661 C→A 14.2% E77* (GAG→TAG)  menI ← 1,4‑dihydroxy‑2‑naphthoyl‑CoA hydrolase
RA 1,778,222 G→T 13.4% G312V (GGA→GTA)  fadK → short chain acyl‑CoA synthetase, anaerobic
RA 1,861,156 C→T 16.0% intergenic (‑427/‑9) mipA ← / → yeaG scaffolding protein for murein synthesizing machinery/protein kinase, endogenous substrate unidentified; autokinase
RA 1,861,588 A→G 16.7% S142G (AGC→GGC)  yeaG → protein kinase, endogenous substrate unidentified; autokinase
RA 1,871,247 C→T 14.8% R28C (CGC→TGC)  yeaO → DUF488 family protein
RA 1,892,493 G→T 22.5% M435I (ATG→ATT sdaA → L‑serine dehydratase 1
RA 1,906,114 G→A 9.3% A240A (GCC→GCT htpX ← putative endopeptidase
JC JC 1,972,762 IS5 (–) +4 bp 93.1% intergenic (‑308/‑469) flhD ← / → uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
RA 1,991,827 A→G 26.7% L45P (CTG→CCG)  yecS ← ABC family putative inner membrane permease
RA 2,015,830 G→T 20.2% G161* (GGA→TGA)  fliP → flagellar biosynthesis protein
RA 2,066,948 G→T 25.0% V643V (GTG→GTT flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,111,960:1 +T 22.2% intergenic (‑75/+201) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,111,963 A→C 24.0% intergenic (‑78/+198) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,111,981 A→G 35.3% intergenic (‑96/+180) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,111,985 G→A 35.4% intergenic (‑100/+176) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,111,989 C→T 37.5% intergenic (‑104/+172) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,111,991 A→T 37.5% intergenic (‑106/+170) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,111,994 Δ1 bp 37.5% intergenic (‑109/+167) wcaK ← / ← wzxC colanic acid biosynthesis protein/putative colanic acid exporter
RA 2,225,607 G→T 14.9% A95A (GCG→GCT cdd → cytidine/deoxycytidine deaminase
RA 2,227,125 C→A 13.1% P16Q (CCG→CAG)  yeiS → DUF2542 family protein
RA 2,233,967 G→T 9.8% intergenic (‑140/+140) mglB ← / ← galS methyl‑galactoside transporter subunit/galactose‑ and fucose‑inducible galactose regulon transcriptional isorepressor; mgl operon transcriptional repressor; autorepressor
RA 2,238,045 G→T 11.2% Q219H (CAG→CAT yeiG → S‑formylglutathione hydrolase
RA 2,244,113 G→T 15.6% L306L (CTG→CTT yeiH → UPF0324 family inner membrane protein
RA 2,263,817 G→T 17.4% M120I (ATG→ATT mepS → murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein
RA 2,269,365 G→T 11.5% W205L (TGG→TTG)  yejE → microcin C transporter YejABEF, permease subunit; ABC family
RA 2,296,021 G→T 9.8% P71Q (CCG→CAG)  napA ← nitrate reductase, periplasmic, large subunit
RA 2,326,819 G→T 14.9% pseudogene (1063/4605 nt) yfaS ← pseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
RA 2,332,758 C→T 9.7% V48I (GTA→ATA)  gyrA ← DNA gyrase (type II topoisomerase), subunit A
RA 2,375,419 G→T 11.8% W111C (TGG→TGT rbn → RNase BN, tRNA processing enzyme
RA 2,394,903 C→T 13.8% G44G (GGG→GGA nuoF ← NADH:ubiquinone oxidoreductase, chain F
RA 2,409,821 G→T 25.2% P532P (CCG→CCT pta → phosphate acetyltransferase
RA 2,433,703 G→T 11.7% intergenic (‑104/+161) yfcJ ← / ← fabB putative arabinose efflux transporter/3‑oxoacyl‑[acyl‑carrier‑protein] synthase I
RA 2,435,149 C→A 11.1% intergenic (‑65/‑94) fabB ← / → mnmC 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I/fused 5‑methylaminomethyl‑2‑thiouridine‑forming enzyme methyltransferase and FAD‑dependent demodification enzyme
RA 2,458,434 C→A 11.5% C18F (TGT→TTT)  mlaA ← ABC transporter maintaining OM lipid asymmetry, OM lipoprotein component
RA 2,483,597 G→A 25.0% G23G (GGC→GGT yfdV ← putative transporter
RA 2,497,314 C→A 30.1% E217D (GAG→GAT fryA ← putative PTS enzyme, Hpr component/enzyme I component/enzyme IIA component
RA 2,630,904 C→A 12.5% E370* (GAA→TAA)  bamB ← BamABCDE complex OM biogenesis lipoprotein
RA 2,711,246 C→A 17.1% M214I (ATG→ATT yfiF ← putative methyltransferase
RA 2,712,898 C→A 25.0% P106Q (CCA→CAA)  yfiP → DTW domain protein
RA 2,717,540 G→T 10.5% M18I (ATG→ATT yfiM → putative lipoprotein
RA 2,726,602 C→A 12.6% V311L (GTA→TTA)  clpB ← protein disaggregation chaperone
RA 2,735,641 C→T 9.1% I136I (ATC→ATT yfiR → putative periplasmic inhibitor of YfiN activity
RA 2,812,350 C→A 22.2% Q52H (CAG→CAT csrA ← pleiotropic regulatory protein for carbon source metabolism
RA 2,853,031 C→A 18.2% intergenic (+18/‑88) mutS → / → pphB methyl‑directed mismatch repair protein/serine/threonine‑specific protein phosphatase 2
RA 2,858,407 C→A 18.3% intergenic (+36/‑53) ygbM → / → ygbN putative hydroxypyruvate isomerase/putative transporter
RA 2,902,577 G→T 7.7% S150Y (TCC→TAC)  pyrG ← CTP synthetase
RA 2,946,848 C→A 19.0% G839W (GGG→TGG)  recB ← exonuclease V (RecBCD complex), beta subunit
RA 2,976,007 T→C 10.5% Q204R (CAA→CGA)  kduD ← hexuronate dehydrogenase; hexuronate utilization in high osmolarity
RA 3,001,506 C→T 10.0% A213V (GCC→GTC)  ygeX → 2,3‑diaminopropionate ammonia lyase, PLP‑dependent
RA 3,018,951 C→A 19.2% P414P (CCC→CCA xanQ → xanthine permease
RA 3,038,704:1 +T 20.0% coding (557/744 nt) ygfF ← putative NAD(P)‑dependent oxidoreductase with NAD(P)‑binding Rossmann‑fold domain
RA 3,049,807 C→T 12.5% P70S (CCC→TCC)  fau → 5‑formyltetrahydrofolate cyclo‑ligase family protein
RA 3,061,418 G→T 8.5% L8M (CTG→ATG)  yggE ← oxidative stress defense protein
RA 3,111,438 C→A 14.4% G345V (GGG→GTG)  yghJ ← DUF4092 family putative lipoprotein peptidase
RA 3,126,064 G→T 22.4% Q170K (CAA→AAA)  yghR ← putative ATP‑binding protein
RA 3,154,808 G→T 10.8% N407K (AAC→AAA ftsP ← septal ring component that protects the divisome from stress; multicopy suppressor of ftsI(Ts)
RA 3,176,768 C→A 28.6% intergenic (+86/+404) zupT → / ← ribB zinc transporter/3,4‑dihydroxy‑2‑butanone‑4‑phosphate synthase
RA 3,196,432 C→T 9.7% R395W (CGG→TGG)  cca → fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase
RA 3,231,393 G→T 10.2% G242C (GGC→TGC)  ygjR → putative NAD(P)‑dependent dehydrogenase
RA 3,309,253 C→A 8.7% E41* (GAG→TAG)  infB ← translation initiation factor IF‑2
RA 3,316,508 C→A 14.3% D308Y (GAC→TAC)  glmM ← phosphoglucosamine mutase
RA 3,414,868 G→T 16.6% W62L (TGG→TTG)  yhdY → putative amino‑acid transporter subunit
RA 3,416,890 T→G 14.7% noncoding (12/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,416,899 A→G 13.1% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,474,724 C→A 30.0% P26H (CCT→CAT)  yheS → putative transporter subunit of ABC superfamily: ATP‑binding component
RA 3,490,893 G→A 14.2% A178T (GCG→ACG)  nirC → nitrite transporter
RA 3,496,562 G→T 7.4% D13Y (GAT→TAT)  frlD → fructoselysine 6‑kinase
RA 3,579,440 C→A 19.1% A248A (GCG→GCT ggt ← gamma‑glutamyltranspeptidase
RA 3,609,035 G→T 12.1% M145I (ATG→ATT nikB → nickel transporter subunit
RA 3,629,574 C→T 12.5% S399S (TCG→TCA yhiN ← putative oxidoreductase
RA 3,650,686 T→A 9.6% I111N (ATT→AAT)  hdeD → acid‑resistance membrane protein
RA 3,663,315 G→T 28.8% E122* (GAG→TAG)  treF → cytoplasmic trehalase
RA 3,669,503 G→T 16.0% intergenic (+35/+147) yhjE → / ← yhjG MFS superfamily sugar transport 1 family protein/putative inner membrane‑anchored periplasmic AsmA family protein
RA 3,703,833 C→A 18.2% M1M (ATG→ATT) † eptB ← KDO phosphoethanolamine transferase, Ca(2+)‑inducible
RA 3,707,269 T→C 26.0% G86G (GGT→GGC yiaC → putative acyl‑CoA transferase
RA 3,714,085 A→T 8.2% N16Y (AAT→TAT)  insJ → IS150 transposase A
RA 3,849,445 A→G 12.5% L75L (TTA→CTA)  yidH ← DUF202 family inner membrane protein
RA 3,854,666 C→A 15.0% pseudogene (682/815 nt) glvG ← pseudogene; probable 6‑phospho‑beta‑glucosidase
RA 3,875,471 G→T 16.8% P71T (CCG→ACG)  dnaN ← DNA polymerase III, beta subunit
RA 3,888,770 A→G 100% I400I (ATT→ATC purP ← adenine permease, high affinity; adenine:H+ symporter
RA 4,042,956 C→T 10.5% I877I (ATC→ATT polA → fused DNA polymerase I 5'‑>3' polymerase/3'‑>5' exonuclease/5'‑>3' exonuclease
RA 4,053,103 G→T 9.5% R446L (CGT→CTT)  typA → GTP‑binding protein
RA 4,064,434 G→T 17.4% P228Q (CCG→CAG)  yihS ← aldose‑ketose isomerase; D‑mannose isomerase
RA 4,140,689 C→T 10.2% E780K (GAA→AAA)  ppc ← phosphoenolpyruvate carboxylase
RA 4,165,449 C→T 23.8% noncoding (50/85 nt) tyrU → tRNA‑Tyr
RA 4,165,450 A→C 23.8% noncoding (51/85 nt) tyrU → tRNA‑Tyr
RA 4,165,886 G→A 13.6% K5K (AAG→AAA tufB → translation elongation factor EF‑Tu 2
RA 4,178,209 C→T 22.2% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 100% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,230,082 C→A 33.7% intergenic (‑99/‑171) yjbT ← / → psiE putative periplasmic protein/phosphate starvation inducible protein
RA 4,268,329 G→T 20.0% intergenic (‑335/‑78) ryjA ← / → yjcD novel sRNA, function unknown/inner membrane putative guanine permease
RA 4,271,228 G→A 12.7% A441T (GCA→ACA)  yjcE → putative cation/proton antiporter
RA 4,303,808 C→A 14.3% P42T (CCT→ACT)  yjdP → putative periplasmic protein
RA 4,307,614 C→A 11.5% A33A (GCG→GCT phnL ← ribophosphonate triphosphate synthase subunit; putative ABC transporter ATP‑binding protein
RA 4,329,084 C→A 19.4% A419S (GCC→TCC)  adiA ← arginine decarboxylase
RA 4,351,354 G→T 20.0% P133H (CCC→CAC)  cadC ← cadBA operon transcriptional activator
RA 4,402,865 T→C 13.9% P7P (CCT→CCC yjfC → ATP‑Grasp family ATPase
RA 4,410,220 G→T 10.9% G142W (GGG→TGG)  ulaA → L‑ascorbate‑specific enzyme IIC permease component of PTS
RA 4,420,655 G→T 12.5% L325L (CTG→CTT cycA → D‑alanine/D‑serine/glycine transporter
RA 4,438,103 G→T 7.7% intergenic (+51/‑161) ytfP → / → chpS GGCT‑like protein/antitoxin of the ChpBS toxin‑antitoxin system
RA 4,475,065 G→T 25.8% S235Y (TCC→TAC)  pepA ← multifunctional aminopeptidase A: a cyteinylglycinase, transcription regulator and site‑specific recombination factor
RA 4,527,581 C→A 9.1% W334C (TGG→TGT nanM ← N‑acetylneuraminic acid mutarotase
RA 4,576,483 G→T 9.5% pseudogene (150/3567 nt) hsdR ← pseudogene, premature stop codon, host restriction; endonuclease R

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 80547 80627 81 3 [1] [0] 3 leuL/leuO leu operon leader peptide/global transcription factor
* * ÷ CP009273 454338 455062 725 3 [0] [1] 2 [lon] [lon]
* * ÷ CP009273 455203 456677 1475 2 [1] [1] 2 lon DNA‑binding ATP‑dependent protease La
* * ÷ CP009273 502044 502060 17 2 [0] [1] 2 [ybaK] [ybaK]
* * ÷ CP009273 578902 579097 196 2 [0] [0] 2 tfaX/appY pseudogene, DLP12 prophage;Phage or Prophage Related/global transcriptional activator; DLP12 prophage
* * ÷ CP009273 703352 703364 13 2 [0] [0] 2 glnS/chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
* * ÷ CP009273 796291 796362 72 2 [1] [1] 2 ybhH putative PrpF family isomerase
* * ÷ CP009273 830382 830394 13 2 [0] [1] 3 dinG ATP‑dependent DNA helicase
* * ÷ CP009273 1146180 1146194 15 3 [1] [0] 3 fabG 3‑oxoacyl‑[acyl‑carrier‑protein] reductase
* * ÷ CP009273 1193235 1193251 17 3 [0] [1] 2 ymfE e14 prophage; putative inner membrane protein
* * ÷ CP009273 1230301 1230308 8 2 [0] [1] 2 nhaB/fadR sodium:proton antiporter/fatty acid metabolism regulon transcriptional regulator
* * ÷ CP009273 1524651 1524678 28 2 [0] [0] 2 [ydcD] [ydcD]
* * ÷ CP009273 1539022 1539044 23 2 [0] [1] 2 yddK pseudogene, leucine‑rich protein; putative glycoportein
* * ÷ CP009273 1557370 1557439 70 2 [0] [1] 3 ddpX/dosP D‑ala‑D‑ala dipeptidase, Zn‑dependent/oxygen sensor, c‑di‑GMP phosphodiesterase, heme‑regulated; cold‑ and stationary phase‑induced bioflim regulator
* * ÷ CP009273 1568021 1568073 53 3 [1] [1] 4 pqqL putative periplasmic M16 family zinc metalloendopeptidase
* * ÷ CP009273 1646672 1646724 53 5 [0] [0] 4 intQ pseudogene, Qin prophage; phage integrase family;Phage or Prophage Related
* * ÷ CP009273 1692508 1692546 39 5 [1] [1] 5 malI transcriptional repressor of Mal regulon
* * ÷ CP009273 1864765 1864866 102 3 [1] [1] 2 yeaI putative membrane‑anchored diguanylate cyclase
* * ÷ CP009273 1888083 1888098 16 8 [1] [0] 4 yoaB/yoaC putative reactive intermediate deaminase/DUF1889 family protein
* * ÷ CP009273 1990311 1990390 80 3 [1] [0] 2 [sdiA] [sdiA]
* * ÷ CP009273 2027278 2027435 158 2 [0] [1] 2 rseX/yedS sRNA antisense regulator of ompA and ompC translation, Hfq‑dependent/pseudogene, outer membrane protein homology; putative outer membrane protein
* * ÷ CP009273 2096907 2096998 92 3 [1] [1] 2 wbbK lipopolysaccharide biosynthesis protein
* * ÷ CP009273 2462634 2462646 13 2 [0] [0] 2 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2462730 2462964 235 2 [0] [1] 2 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2463629 2463879 251 2 [1] [1] 2 gtrS serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
* * ÷ CP009273 2623001 2623013 13 2 [0] [1] 2 yfgG/yfgH uncharacterized protein/outer membrane integrity lipoprotein
* * ÷ CP009273 2767881 2768076 196 4 [1] [0] 4 yfjW CP4‑57 prophage; putative inner membrane protein
* * ÷ CP009273 2909984 2910013 30 2 [0] [0] 2 barA hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
* * ÷ CP009273 2988464 2988541 78 5 [1] [1] 2 ygeK/ygeN pseudogene; putative 2‑component transcriptional regulator/pseudogene
* * ÷ CP009273 2989058 2989087 30 2 [1] [1] 2 ygeN pseudogene
* * ÷ CP009273 3406950 3406969 20 2 [0] [1] 2 acrS/acrE acrAB operon transcriptional repressor/cytoplasmic membrane lipoprotein
* * ÷ CP009273 3629270 3629301 32 2 [0] [1] 2 yhiM/yhiN acid resistance protein, inner membrane/putative oxidoreductase
* * ÷ CP009273 3745266 3745346 81 5 [1] [0] 2 yiaT/yiaU putative outer membrane protein/putative DNA‑binding transcriptional regulator
* * ÷ CP009273 3762929 3763190 262 2 [1] [1] 2 [yibV]–yibV [yibV],yibV
* * ÷ CP009273 3792630 3792801 172 2 [1] [1] 3 [waaU]–[waaZ] [waaU],[waaZ]
* * ÷ CP009273 3797657 3797719 63 3 [0] [0] 2 waaS lipopolysaccharide core biosynthesis protein
* * ÷ CP009273 3798255 3798456 202 2 [1] [1] 3 waaS lipopolysaccharide core biosynthesis protein
* * ÷ CP009273 3830068 3830237 170 3 [1] [1] 2 yicT/setC pseudogene/putative arabinose efflux transporter
* * ÷ CP009273 4037430 4037540 111 3 [1] [0] 2 dsbA/yihF periplasmic protein disulfide isomerase I/DUF945 family protein
* * ÷ CP009273 4294341 4294386 46 3 [0] [1] 2 ytcA/yjcS putative inner membrane DUF1656 family protein; efflux pump associated/metallo‑beta‑lactamase superfamily protein
* * ÷ CP009273 4466693 4466841 149 2 [1] [0] 2 yjgL uncharacterized protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 = 269817NA (NA)4 (0.190) 3/246 NT NA noncoding (1044/1195 nt) IS5 repeat region
?CP009273 269869 = NA (NA)noncoding (992/1195 nt) IS5 repeat region
* ? CP009273 360104 =5 (0.230)13 (0.600) 7/248 NT 72.2% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =15 (0.690)5 (0.270) 3/216 NT 36.9% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 4 (0.210)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326113 (0.600)5 (0.270) 3/216 NT 39.5% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 4 (0.210)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 4532499 =2 (0.090)5 (0.250) 3/230 NT 40.2% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?CP009273 4532813 = 13 (0.650)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)