breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
RA 70,356 C→A 6.9% G218V (GGG→GTG) ‡ thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 70,357 C→T 6.9% G218R (GGG→AGG) ‡ thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 70,363 Δ1 bp 6.1% coding (646/1611 nt) thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 70,364 Δ1 bp 6.1% coding (645/1611 nt) thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 70,365 A→G 6.0% L215P (CTA→CCA)  thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 70,368 Δ1 bp 5.5% coding (641/1611 nt) thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 70,370 Δ1 bp 5.8% coding (639/1611 nt) thiP ← thiamine, thiamine pyrophosphate ABC transporter permease; ThiBPQ thiamine, thiamine pyrophosphate importer
RA 88,592 C→A 13.6% N231K (AAC→AAA ftsI → transpeptidase involved in septal peptidoglycan synthesis (penicillin‑binding protein 3)
RA 93,307 A→G 5.2% L273L (TTA→TTG mraY → phospho‑N‑acetylmuramoyl‑pentapeptide transferase
RA 95,552 G→A 5.1% Q221Q (CAG→CAA ftsW → lipid II flippase; integral membrane protein involved in stabilizing FstZ ring during cell division
RA 141,748 G→A 6.8% A61T (GCA→ACA)  yadE → putative polysaccharide deacetylase lipoprotein
RA 159,229 G→T 6.8% G213V (GGA→GTA)  hrpB → putative ATP‑dependent helicase
RA 179,823 C→A 5.6% P292T (CCT→ACT)  cdaR → carbohydrate diacid regulon transcriptional regulator; autoregulator
RA 180,552 T→C 5.4% T11A (ACC→GCC)  yaeH ← UPF0325 family protein
RA 195,817 A→G 5.5% Y468C (TAT→TGT)  bamA → BamABCDE complex OM biogenesis outer membrane pore‑forming assembly factor
RA 221,407 A→T 5.2% noncoding (1150/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,412 A→T 5.2% noncoding (1155/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,420 A→G 5.4% noncoding (1163/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,425 T→C 8.3% noncoding (1168/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 221,430 T→C 8.6% noncoding (1173/1542 nt) rrsH → 16S ribosomal RNA of rrnH operon
RA 249,908 A→G 11.5% intergenic (+260/‑58) yafP → / → ykfJ putative acyl‑CoA transferase/pseudogene
RA 260,759 C→A 5.6% W53C (TGG→TGT yafX ← CP4‑6 prophage; uncharacterized protein
RA 277,094 G→A 5.1% P201S (CCG→TCG)  yagA ← CP4‑6 prophage; putative DNA‑binding transcriptional regulator
RA 286,409 T→A 7.7% Q152L (CAG→CTG)  insB1 ← IS1 transposase B
RA 337,136 G→T 6.4% A101S (GCC→TCC)  yahJ → putative metallo‑dependent hydrolase domain deaminase
RA 372,887:1 +A 6.1% intergenic (+120/+104) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 372,888 C→G 6.1% intergenic (+121/+103) yaiL → / ← frmB DUF2058 family protein/S‑formylglutathione hydrolase
RA 420,091 T→C 5.5% D95G (GAC→GGC)  acpH ← acyl carrier protein (ACP) phosphodiesterase; ACP hydrolyase
RA 431,417 C→T 6.9% L110F (CTT→TTT)  thiL → thiamine monophosphate kinase
RA 478,824 C→A 6.0% E346* (GAA→TAA)  acrB ← multidrug efflux system protein
RA 520,562 C→A 6.5% R616R (CGG→AGG)  rhsD → Rhs family putative polymorphic toxin
RA 520,636 G→A 5.9% E640E (GAG→GAA rhsD → Rhs family putative polymorphic toxin
RA 520,654 T→G 5.3% R646R (CGT→CGG rhsD → Rhs family putative polymorphic toxin
RA 520,661 A→C 5.8% S649R (AGC→CGC)  rhsD → Rhs family putative polymorphic toxin
RA 573,883 G→C 5.3% T107T (ACG→ACC
R34P (CGG→CCG) 
rzpD →
rzoD →
DLP12 prophage; putative murein endopeptidase
DLP12 prophage; putative lipoprotein
RA 592,104 C→A 5.5% A350D (GCC→GAC)  cusC → copper/silver efflux system, outer membrane component
RA 627,941 G→A 7.8% T331T (ACC→ACT ybdH ← putative oxidoreductase
RA 648,598 G→T 7.7% R303L (CGG→CTG)  citA → sensory histidine kinase in two‑component regulatory system with CitB
RA 649,469 C→T 5.9% P51L (CCG→CTG)  citB → response regulator in two‑component regulatory system with CitA
RA 654,377 G→T 5.7% intergenic (+103/‑26) ybeM → / → tatE pseudogene, C‑N hydrolase superfamily homology;putative enzyme; Not classified; putative amidase/TatABCE protein translocation system subunit
RA 701,992 C→T 5.1% D148D (GAC→GAT glnS → glutamyl‑tRNA synthetase
RA 727,171 T→G 5.9% N711K (AAT→AAG rhsC → Rhs family putative polymorphic toxin
RA 727,207 C→T 7.9% R723R (CGC→CGT rhsC → Rhs family putative polymorphic toxin
RA 727,989 C→A 8.6% A984E (GCA→GAA)  rhsC → Rhs family putative polymorphic toxin
RA 728,560 T→C 7.5% G1174G (GGT→GGC rhsC → Rhs family putative polymorphic toxin
RA 729,930 A→G 6.6% pseudogene (342/1521 nt) rhsO → pseudogene, Rhs family protein
RA 783,126 T→C 6.6% intergenic (‑75/+127) gpmA ← / ← galM phosphoglyceromutase 1/aldose 1‑epimerase; type‑1 mutarotase
RA 897,023 C→A 7.1% L228L (CTG→CTT artQ ← arginine transporter subunit
RA 908,193 C→A 6.3% A360S (GCA→TCA)  hcp ← hybrid‑cluster [4Fe‑2S‑2O] protein in anaerobic terminal reductases
RA 946,992 G→T 5.5% N673K (AAC→AAA pflB ← formate C‑acetyltransferase 1, anaerobic; pyruvate formate‑lyase 1
RA 963,006 C→T 7.7% R310R (CGC→CGT msbA → fused lipid transporter subunits of ABC superfamily: membrane component/ATP‑binding component
RA 998,746 G→A 8.0% P134P (CCG→CCA ycbU → putative fimbriae protein
RA 1,029,072 G→T 6.6% V121V (GTG→GTT hyaB → hydrogenase 1, large subunit
RA 1,056,237 C→G 8.2% A509G (GCC→GGC)  torA → trimethylamine N‑oxide (TMAO) reductase I, catalytic subunit
RA 1,105,460 C→T 6.9% P224S (CCA→TCA)  opgG → osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
RA 1,214,547 C→A 8.4% L37M (CTG→ATG)  ymgF → inner membrane division septum protein
RA 1,259,313 G→A 6.1% S48S (TCG→TCA hemA → glutamyl tRNA reductase
RA 1,259,400 C→A 14.4% H77Q (CAC→CAA hemA → glutamyl tRNA reductase
RA 1,265,875 T→C 10.8% noncoding (27/68 nt) rdlC → sRNA antisense regulator affects LdrC translation; proposed addiction module in LDR‑C repeat, with toxic peptide LdrC
RA 1,282,249 A→G 15.9% intergenic (+267/+273) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
RA 1,282,599 G→A 10.6% noncoding (94/171 nt)
P12S (CCA→TCA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,282,608 T→C 11.4% noncoding (85/171 nt)
T9A (ACT→GCT) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,282,617 G→A 10.7% noncoding (76/171 nt)
Q6* (CAA→TAA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein
RA 1,282,676 A→G 5.2% noncoding (17/171 nt) rttR ← rtT sRNA, processed from tyrT transcript
RA 1,287,049 T→C 8.4% V46A (GTG→GCG)  galU → glucose‑1‑phosphate uridylyltransferase
RA 1,368,394 G→T 5.8% G16G (GGG→GGT ycjP → inner membrane putative ABC superfamily sugar transporter permease
RA 1,387,970 G→T 8.8% A163S (GCC→TCC)  mppA → murein tripeptide (L‑ala‑gamma‑D‑glutamyl‑meso‑DAP) transporter subunit
RA 1,408,527 G→A 9.7% A175V (GCT→GTT)  recT ← Rac prophage; recombination and repair protein
RA 1,422,040 A→G 11.0% pseudogene (944/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,060 G→C 10.8% pseudogene (924/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,098 G→A 5.0% pseudogene (886/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,219 C→T 5.7% pseudogene (765/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,224 T→G 7.5% pseudogene (760/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,228 C→T 7.4% pseudogene (756/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,627 A→G 7.0% pseudogene (357/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,422,648 G→C 7.8% pseudogene (336/981 nt) insH1 ← IS5 transposase and trans‑activator;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 1,432,957 G→A 6.0% C695C (TGC→TGT pfo ← pyruvate‑flavodoxin oxidoreductase
RA 1,457,738 G→A 8.3% intergenic (+43/‑58) paaK → / → paaX phenylacetyl‑CoA ligase/transcriptional repressor of phenylacetic acid degradation paa operon, phenylacetyl‑CoA inducer
RA 1,460,752 C→T 10.1% pseudogene (1104/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,460,761 A→G 11.3% pseudogene (1113/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,467,973 C→A 9.5% pseudogene (3200/3497 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,510,174 G→T 9.4% A114S (GCG→TCG)  patD → gamma‑aminobutyraldehyde dehydrogenase
RA 1,522,209 C→T 5.7% pseudogene (51/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,522,243 C→T 6.9% pseudogene (85/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,523,601 G→A 10.3% pseudogene (1443/2037 nt) rhsE → pseudogene, Rhs family protein
RA 1,526,984 T→C 5.9% H304H (CAT→CAC ydcC → H repeat‑associated putative transposase
RA 1,535,098 G→A 6.6% R584C (CGC→TGC)  narZ ← nitrate reductase 2 (NRZ), alpha subunit
RA 1,586,506 T→A 9.3% E65V (GAA→GTA)  hipB ← antitoxin of HipAB toxin‑antitoxin system
RA 1,618,622 C→A 7.9% D45Y (GAT→TAT)  ydeI ← hydrogen peroxide resistance OB fold protein; putative periplasmic protein
RA 1,629,727 A→G 5.3% V126V (GTT→GTC) ‡ stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,728 A→G 5.3% V126A (GTT→GCT) ‡ stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,629,823 A→G 6.4% G94G (GGT→GGC stfQ ← Qin prophage; putative side tail fiber assembly protein
RA 1,641,732 C→A 6.9% pseudogene (162/279 nt) ydfX ← pseudogene, Qin prophage;Phage or Prophage Related
RA 1,657,580 G→A 5.1% G112S (GGC→AGC)  ynfG → oxidoreductase, Fe‑S subunit
RA 1,673,103 C→A 9.0% I140I (ATC→ATA ydgH → DUF1471 family periplasmic protein
RA 1,702,935 G→A 9.8% R587H (CGC→CAC)  rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,702,966 T→A 12.1% R597R (CGT→CGA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,725,778 T→C 10.5% L812P (CTG→CCG)  lhr → putative ATP‑dependent helicase
RA 1,732,878 G→T 6.9% E260* (GAA→TAA)  purR → transcriptional repressor, hypoxanthine‑binding
RA 1,752,177 G→A 8.3% T269I (ACA→ATA)  ldtE ← murein L,D‑transpeptidase
RA 1,757,417 C→T 8.2% V284I (GTA→ATA)  sufB ← component of SufBCD Fe‑S cluster assembly scaffold
RA 1,763,146 C→A 9.9% intergenic (‑204/‑185) ydiJ ← / → ydiK putative FAD‑linked oxidoreductase/UPF0118 family inner membrane protein
RA 1,789,064 C→T 5.3% A116T (GCG→ACG)  btuC ← vitamin B12 transporter subunit: membrane component of ABC superfamily
RA 1,841,797 C→T 6.2% V157I (GTA→ATA)  selD ← selenophosphate synthase
RA 1,869,403 T→A 6.2% Q144L (CAG→CTG)  yeaM ← putative DNA‑binding transcriptional regulator
RA 1,931,572 G→T 12.1% A222S (GCA→TCA)  yebK → putative DNA‑binding transcriptional regulator
RA 1,961,109 G→A 7.7% T62T (ACC→ACT cheZ ← chemotaxis regulator, protein phosphatase for CheY
RA 2,009,308 C→T 5.8% A316A (GCC→GCT fliG → flagellar motor switching and energizing component
RA 2,009,907 C→A 5.7% R187S (CGC→AGC)  fliH → negative regulator of FliI ATPase activity
RA 2,013,547 C→A 6.2% A150D (GCT→GAT)  fliL → flagellar biosynthesis protein
RA 2,019,914 C→A 6.4% E529* (GAG→TAG)  yedQ ← putative membrane‑anchored diguanylate cyclase
RA 2,067,603 G→T 8.5% A862S (GCC→TCC)  flu → CP4‑44 prophage; antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
RA 2,133,928 C→T 7.0% G389D (GGC→GAC)  asmA ← suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
RA 2,249,594 A→G 5.2% T407T (ACT→ACC psuT ← putative nucleoside transporter
RA 2,249,608 G→A 5.3% L403L (CTG→TTG)  psuT ← putative nucleoside transporter
RA 2,249,621 T→C 7.8% A398A (GCA→GCG psuT ← putative nucleoside transporter
RA 2,249,624 T→C 7.8% A397A (GCA→GCG psuT ← putative nucleoside transporter
RA 2,249,627 T→C 7.8% A396A (GCA→GCG psuT ← putative nucleoside transporter
RA 2,257,691 G→T 6.1% S117I (AGC→ATC)  setB → lactose/glucose efflux system
RA 2,272,928 C→A 8.1% M104I (ATG→ATT bcr ← bicyclomycin/cysteine/sulfonamide efflux transporter
RA 2,307,842 G→A 5.7% L292L (CTG→CTA rcsD → phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
RA 2,370,570 G→T 5.4% R168S (CGC→AGC)  menH ← 2‑succinyl‑6‑hydroxy‑2, 4‑cyclohexadiene‑1‑carboxylate synthase
RA 2,374,398 C→T 5.5% V37I (GTT→ATT)  elaB ← DUF883 family protein, putative membrane‑anchored ribosome‑binding protein
RA 2,393,394 G→T 13.4% A84E (GCG→GAG)  nuoG ← NADH:ubiquinone oxidoreductase, chain G
RA 2,438,934 C→A 6.2% G36W (GGG→TGG)  yfcA ← UPF0721 family inner membrane protein
RA 2,471,079 C→T 10.5% R10R (CGG→CGA dsdC ← dsd operon activator; autorepressor
RA 2,516,832 C→A 7.6% L171L (CTG→CTT xapB ← xanthosine transporter
RA 2,520,863 G→T 6.6% D187Y (GAC→TAC)  yfeH → putative inorganic ion transporter
RA 2,530,059 C→T 9.3% A180T (GCC→ACC)  pdxK ← pyridoxal‑pyridoxamine kinase/hydroxymethylpyrimidine kinase
RA 2,534,658 A→G 6.7% S127S (TCT→TCC cysW ← sulfate/thiosulfate ABC transporter subunit
RA 2,540,398 C→A 6.8% P123T (CCG→ACG)  murP → N‑acetylmuramic acid permease, EIIBC component, PTS system
RA 2,564,645 C→A 7.4% V156F (GTC→TTC)  eutE ← aldehyde oxidoreductase, ethanolamine utilization protein
RA 2,574,648 A→G 8.0% I552V (ATT→GTT)  tktB → transketolase 2, thiamine triphosphate‑binding
RA 2,619,134 A→T 7.5% K221* (AAA→TAA)  ppx → exopolyphosphatase
RA 2,630,508 G→T 5.3% R70S (CGC→AGC)  der ← GTPase; multicopy suppressor of ftsJ
RA 2,722,960 T→A 6.8% intergenic (‑157/+15) gltW ← / ← rrsG tRNA‑Glu/16S ribosomal RNA of rrnG operon
RA 2,757,696 C→A 7.8% V273F (GTC→TTC)  yfjL ← CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein
RA 2,762,984 T→G 9.9% intergenic (+139/‑78) yfjQ → / → yfjR CP4‑57 prophage; uncharacterized protein/CP4‑57 prophage; putative DNA‑binding transcriptional regulator
RA 2,784,260 G→T 8.5% R307L (CGC→CTC)  lhgO → L‑2‑hydroxyglutarate oxidase
RA 2,790,576 G→T 6.1% E3* (GAA→TAA)  ygaV → tributyltin‑inducible repressor of ygaVP
RA 2,805,658 T→C 5.5% G291G (GGT→GGC emrA → multidrug efflux system
RA 2,805,659:1 +C 5.3% coding (874/1173 nt) emrA → multidrug efflux system
RA 2,805,659:2 +G 5.3% coding (874/1173 nt) emrA → multidrug efflux system
RA 2,805,659:3 +C 5.3% coding (874/1173 nt) emrA → multidrug efflux system
RA 2,805,659:4 +A 5.3% coding (874/1173 nt) emrA → multidrug efflux system
RA 2,805,660 T→C 5.3% L292P (CTG→CCG)  emrA → multidrug efflux system
RA 2,805,663 A→C 7.3% D293A (GAT→GCT)  emrA → multidrug efflux system
RA 2,805,664:1 +G 6.6% coding (879/1173 nt) emrA → multidrug efflux system
RA 2,805,666 T→A 6.6% M294K (ATG→AAG) ‡ emrA → multidrug efflux system
RA 2,805,667 G→A 6.3% M294I (ATG→ATA) ‡ emrA → multidrug efflux system
RA 2,809,704 C→T 6.4% R32H (CGT→CAT)  gshA ← glutamate‑cysteine ligase
RA 2,811,683 G→A 5.7% intergenic (‑50/+149) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,811,690 G→T 7.0% intergenic (‑57/+142) argY ← / ← argV tRNA‑Arg/tRNA‑Arg
RA 2,842,897 C→A 6.1% P104P (CCG→CCT hycB ← hydrogenase 3, Fe‑S subunit
RA 2,888,886 A→G 7.5% C85R (TGC→CGC)  ygcQ ← putative flavoprotein
RA 2,904,833 C→A 5.4% L726L (CTG→CTT relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,905,031 G→T 5.4% S660R (AGC→AGA relA ← (p)ppGpp synthetase I/GTP pyrophosphokinase
RA 2,940,824 Δ1 bp 7.0% intergenic (+2/‑32) metZ → / → metW tRNA‑Met/tRNA‑Met
RA 2,940,826 A→C 7.0% intergenic (+4/‑30) metZ → / → metW tRNA‑Met/tRNA‑Met
RA 2,940,902 G→A 5.2% noncoding (47/77 nt) metW → tRNA‑Met
RA 2,981,545 G→T 5.9% intergenic (+18/‑316) yqeH → / → yqeI putative LuxR family transcriptional regulator/putative transcriptional regulator
RA 3,002,965 C→T 5.6% A281A (GCC→GCT ygeY → putative peptidase
RA 3,030,035 C→A 9.3% M477I (ATG→ATT recJ ← ssDNA exonuclease, 5' ‑‑> 3'‑specific
RA 3,053,356 G→A 8.5% G82D (GGC→GAC)  argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,053,359 Δ1 bp 9.1% coding (248/894 nt) argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,053,360 T→C 9.1% D83D (GAT→GAC argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,053,361 G→A 9.1% E84K (GAA→AAA) ‡ argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,053,362 A→T 8.9% E84V (GAA→GTA) ‡ argP → transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
RA 3,063,416 T→G 5.1% intergenic (‑250/+108) mscS ← / ← fbaA mechanosensitive channel protein, small conductance/fructose‑bisphosphate aldolase, class II
RA 3,064,664 T→G 5.1% intergenic (‑61/+154) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,064,669 G→A 5.2% intergenic (‑66/+149) fbaA ← / ← pgk fructose‑bisphosphate aldolase, class II/phosphoglycerate kinase
RA 3,133,045 G→T 5.8% intergenic (+93/+30) yghU → / ← hybG putative S‑transferase/hydrogenase 2 accessory protein
RA 3,149,035 G→T 5.7% A108S (GCT→TCT)  yqhD → aldehyde reductase, NADPH‑dependent
RA 3,164,938 C→T 5.2% R366C (CGC→TGC)  qseC → quorum sensing sensory histidine kinase in two‑component regulatory system with QseB
RA 3,173,328 G→A 5.7% E76K (GAA→AAA)  ygiB → DUF1190 family protein
RA 3,263,315 T→C 6.2% intergenic (+354/+260) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component
RA 3,288,784 C→A 10.9% P676T (CCC→ACC) 
P11H (CCC→CAC) 
lpoA →
yraN →
OM lipoprotein stimulator of MrcA transpeptidase
UPF0102 family protein
RA 3,295,709 G→T 6.0% R289L (CGT→CTT)  yhbU → U32 peptidase family protein
RA 3,311,602 T→C 14.1% noncoding (47/77 nt) metY ← tRNA‑Met
RA 3,338,415 G→T 5.6% G114C (GGC→TGC)  rpoN → RNA polymerase, sigma 54 (sigma N) factor
RA 3,345,913 G→T 6.4% R158S (CGT→AGT)  arcB ← aerobic respiration control sensor histidine protein kinase, cognate to two‑component response regulators ArcA and RssB
RA 3,350,795 G→T 6.7% V904V (GTG→GTT gltB → glutamate synthase, large subunit
RA 3,385,539 G→A 5.9% intergenic (‑152/+278) tldD ← / ← yhdP putative peptidase/DUF3971‑AsmA2 domains protein
RA 3,392,512 C→A 5.5% G276C (GGC→TGC)  mreC ← cell wall structural complex MreBCD transmembrane component MreC
RA 3,416,899 A→G 5.8% noncoding (3/120 nt) rrfF ← 5S ribosomal RNA of rrnD operon
RA 3,470,686 A→G 5.1% P31P (CCT→CCC fkpA ← FKBP‑type peptidyl‑prolyl cis‑trans isomerase (rotamase)
RA 3,473,064 C→T 5.3% L301L (CTG→CTA kefB ← potassium:proton antiporter
RA 3,473,917 G→A 5.9% A17V (GCG→GTG)  kefB ← potassium:proton antiporter
RA 3,515,094 C→A 8.0% E80* (GAA→TAA)  hofN ← DNA catabolic putative fimbrial assembly protein
RA 3,611,324 G→A 5.1% S63S (TCG→TCA nikE → nickel transporter subunit
RA 3,614,441 G→T 5.3% G630G (GGG→GGT rhsB → Rhs family putative polymorphic toxin, putative neighboring cell growth inhibitor
RA 3,618,160 A→G 14.2% G141G (GGA→GGG yhhI → putative transposase
RA 3,632,623 G→T 12.0% intergenic (+122/+122) pitA → / ← uspB phosphate transporter, low‑affinity; tellurite importer/universal stress (ethanol tolerance) protein B
RA 3,660,797 A→G 6.5% D48D (GAT→GAC gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,660,812 A→G 5.3% F43F (TTT→TTC gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,678,745 C→A 8.6% P78P (CCG→CCT yhjK ← cyclic‑di‑GMP phosphodiesterase
RA 3,822,340 A→C 11.9% Q12H (CAA→CAC xanP → xanthine permease
RA 3,833,019 A→G 5.6% V112A (GTC→GCC)  nlpA ← cytoplasmic membrane lipoprotein‑28
RA 3,949,697 Δ1 bp 7.4% coding (1007/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,698 Δ1 bp 7.4% coding (1008/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,699 Δ1 bp 7.4% coding (1009/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,700 Δ1 bp 7.4% coding (1010/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,701 Δ1 bp 7.4% coding (1011/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,703 Δ1 bp 7.0% coding (1013/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,704 Δ1 bp 7.0% coding (1014/1545 nt) ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,707 G→T 7.3% A339A (GCG→GCT ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,712 T→A 7.4% L341* (TTG→TAG) ‡ ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,949,713 G→A 7.7% L341L (TTG→TTA) ‡ ilvA → L‑threonine dehydratase, biosynthetic; also known as threonine deaminase
RA 3,976,013 C→T 7.5% noncoding (48/87 nt) leuT → tRNA‑Leu
RA 3,996,977 G→A 5.6% P188S (CCC→TCC)  rarD ← putative chloramphenical resistance permease
RA 4,000,259 G→T 8.5% A346S (GCG→TCG)  recQ → ATP‑dependent DNA helicase
RA 4,003,420 G→T 9.2% P297P (CCG→CCT pldB → lysophospholipase L2
RA 4,019,937 C→T 5.2% T17T (ACC→ACT fre → NAD(P)H‑flavin reductase
RA 4,042,364 C→T 6.3% P680L (CCG→CTG)  polA → fused DNA polymerase I 5'‑>3' polymerase/3'‑>5' exonuclease/5'‑>3' exonuclease
RA 4,063,817 G→T 10.1% intergenic (‑56/+58) yihR ← / ← yihS mutarotase superfamily protein, YihR family/aldose‑ketose isomerase; D‑mannose isomerase
RA 4,063,823 T→A 11.5% intergenic (‑62/+52) yihR ← / ← yihS mutarotase superfamily protein, YihR family/aldose‑ketose isomerase; D‑mannose isomerase
RA 4,063,824 T→A 11.6% intergenic (‑63/+51) yihR ← / ← yihS mutarotase superfamily protein, YihR family/aldose‑ketose isomerase; D‑mannose isomerase
RA 4,076,678 A→G 8.5% R835R (CGT→CGC fdoG ← formate dehydrogenase‑O, large subunit
RA 4,076,681 C→T 8.7% G834G (GGG→GGA fdoG ← formate dehydrogenase‑O, large subunit
RA 4,076,687 G→C 9.0% G832G (GGC→GGG fdoG ← formate dehydrogenase‑O, large subunit
RA 4,076,694 T→G 9.9% Q830P (CAG→CCG)  fdoG ← formate dehydrogenase‑O, large subunit
RA 4,087,233 C→A 7.3% pseudogene (118/543 nt) rhaD ← pseudogene, truncated, rhamnulose‑phosphate aldolase
RA 4,091,587 G→T 6.0% intergenic (+229/‑31) sodA → / → kdgT superoxide dismutase, Mn/2‑keto‑3‑deoxy‑D‑gluconate transporter
RA 4,143,298 T→C 6.3% intergenic (‑272/+326) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,143,340 T→C 7.8% intergenic (‑314/+284) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase
RA 4,149,083 C→A 8.0% T222T (ACC→ACA oxyR → oxidative and nitrosative stress transcriptional regulator
RA 4,161,576 A→C 14.0% noncoding (12/120 nt) rrfB → 5S ribosomal RNA of rrnB operon
RA 4,175,207 G→T 8.6% intergenic (+6/‑71) rpoB → / → rpoC RNA polymerase, beta subunit/RNA polymerase, beta prime subunit
RA 4,178,209 C→T 37.2% R978C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,178,500 C→T 47.5% R1075C (CGT→TGT)  rpoC → RNA polymerase, beta prime subunit
RA 4,182,687 G→T 8.9% Q27K (CAA→AAA)  thiS ← immediate sulfur donor in thiazole formation
RA 4,202,979 A→C 22.6% noncoding (12/120 nt) rrfE → 5S ribosomal RNA of rrnE operon
RA 4,207,374 C→T 5.3% P113L (CCG→CTG)  aceA → isocitrate lyase
RA 4,238,262 G→T 5.6% G122C (GGC→TGC)  lamB → maltose outer membrane porin (maltoporin)
RA 4,285,988 C→T 11.0% intergenic (+266/+265) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,304,378 G→T 5.5% P181Q (CCG→CAG)  phnP ← 5‑phospho‑alpha‑D‑ribosyl 1,2‑cyclic phosphate phosphodiesterase
RA 4,315,705 G→T 6.1% intergenic (‑147/+511) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,434,343 G→A 5.2% A139T (GCA→ACA)  tamB → translocation and assembly module for autotransporter export, inner membrane subunit
RA 4,447,037 T→A 7.4% intergenic (+62/+94) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,039 T→C 6.1% intergenic (+64/+92) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,041 A→T 6.2% intergenic (+66/+90) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,045 T→C 6.1% intergenic (+70/+86) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,047 G→A 6.1% intergenic (+72/+84) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,063 Δ1 bp 6.2% intergenic (+88/+68) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,066 A→C 6.1% intergenic (+91/+65) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,447,067 T→C 6.1% intergenic (+92/+64) mpl → / ← yjgA UDP‑N‑acetylmuramate:L‑alanyl‑gamma‑D‑glutamyl‑ meso‑diaminopimelate ligase/ribosome‑associated UPF0307 family protein
RA 4,463,327 C→G 6.2% D182H (GAC→CAC)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,478,341 C→T 5.5% intergenic (+124/+37) lptG → / ← yjgR lipopolysaccharide export ABC permease of the LptBFGC export complex/DUF853 family protein with NTPase fold
RA 4,499,743 C→A 9.3% pseudogene (123/330 nt) yjhV → pseudogene, KpLE2 phage‑like element
RA 4,508,444 C→A 9.1% P34H (CCT→CAT) ‡ insA → IS1 repressor TnpA
RA 4,508,445 T→C 8.3% P34P (CCT→CCC) ‡ insA → IS1 repressor TnpA
RA 4,508,690 T→G 5.7% pseudogene (153/504 nt) insB1 → IS1 transposase B;IS, phage, Tn; Transposon‑related functions; extrachromosomal; transposon related
RA 4,604,194 C→T 5.3% intergenic (+117/+303) yjjV → / ← yjjW putative DNase/putative pyruvate formate lyase activating enzyme

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 3448780 3448880 101 16 [15] [15] 20 gspA/gspC general secretory pathway component, cryptic/general secretory pathway component, cryptic
* * ÷ CP009273 3763078 3763264 187 16 [14] [15] 20 [yibV] [yibV]
* * ÷ CP009273 4249412 4249976 565 16 [15] [15] 17 zur zur

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 335746 =61 (0.840)8 (0.110) 5/246 NT 11.7% noncoding (279/358 nt) REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences REP23 (repetitive extragenic palindromic) element; contains 8 REP sequences
?CP009273 372778 = NA (NA)intergenic (+11/+213) yaiL/frmB DUF2058 family protein/S‑formylglutathione hydrolase
* ? CP009273 360104 =53 (0.730)50 (0.690) 31/248 NT 48.5% intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 37038576 (1.450)22 (0.470) 10/162 NT 24.3% intergenic (+48/‑530) mhpE/mhpT 4‑hyroxy‑2‑oxovalerate/4‑hydroxy‑2‑oxopentanoic acid aldolase, class I/3‑hydroxyphenylpropionic transporter
?CP009273 = 372977 NA (NA)noncoding (187/187 nt) REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences REP32 (repetitive extragenic palindromic) element; contains 4 REP sequences
* ? CP009273 = 627604NA (NA)3 (0.060) 3/172 NT 8.9% noncoding (34/34 nt) REP55 (repetitive extragenic palindromic) element; contains 1 REP sequences REP55 (repetitive extragenic palindromic) element; contains 1 REP sequences
?CP009273 2284778 = 31 (0.610)noncoding (147/199 nt) RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site RIP160 (repetitive extragenic palindromic) element; contains 4 REP sequences and 1 IHF site
* ? CP009273 1203246 =33 (0.460)23 (0.370) 14/216 NT 41.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 36 (0.570)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326131 (0.430)17 (0.270) 12/216 NT 35.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 36 (0.570)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 197277743 (0.600)4 (0.060) 3/248 NT 8.5% intergenic (‑323/‑457) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 2283592NA (NA)105 (1.480) 34/244 NT 89.7% noncoding (1/1195 nt) IS5 repeat region
?CP009273 2399636 = 12 (0.170)coding (485/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
* ? CP009273 = 239963912 (0.170)72 (1.000) 32/248 NT 85.7% coding (482/939 nt) lrhA transcriptional repressor of flagellar, motility and chemotaxis genes
?CP009273 = 3124699 NA (NA)intergenic (+146/+1) insH1/yghQ IS5 transposase and trans‑activator/putative inner membrane polysaccharide flippase