breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 3,118 G→A A106A (GCG→GCA thrB → homoserine kinase
RA 27,613 T→A I107I (ATT→ATA rihC → ribonucleoside hydrolase RihC
RA 81,437 A→G R174R (CGT→CGC leuB ← 3‑isopropylmalate dehydrogenase
RA 89,890 T→A M86K (ATG→AAG)  mraZ → DNA‑binding transcriptional repressor MraZ
RA 94,873 T→A L75Q (CTG→CAG)  murF → D‑alanyl‑D‑alanine‑adding enzyme
RA 134,658 A→G N91D (AAC→GAC)  yacL → UPF0231 family protein YacL
RA 148,936 G→A T222T (ACC→ACT panB ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
RA 153,617 T→C S604G (AGT→GGT)  htrE ← putative fimbrial usher protein HtrE
RA 157,376 C→T M119I (ATG→ATA folK ← 2‑amino‑4‑hydroxy‑6‑ hydroxymethyldihydropteridine diphosphokinase
RA 168,655 Δ1 bp coding (1172/2244 nt) fhuA → ferrichrome outer membrane transporter/phage receptor
RA 215,261 A→T intergenic (+6/‑8) arfB → / → nlpE peptidyl‑tRNA hydrolase, ribosome rescue factor/lipoprotein NlpE
RA 218,480 C→T R99H (CGT→CAT)  proS ← proline‑‑tRNA ligase
RA 233,614 C→T M114I (ATG→ATA mltD ← membrane‑bound lytic murein transglycosylase D
RA 236,552 C→A H162Q (CAC→CAA dnaQ → DNA polymerase III subunit epsilon
RA 245,180 C→A R209L (CGT→CTT)  yafK ← L,D‑transpeptidase domain‑containing protein YafK
RA 251,954 T→C intergenic (+1/‑51) dinB → / → yafN DNA polymerase IV/antitoxin YafN
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 294,118 G→A L228F (CTT→TTT)  yagM ← CP4‑6 prophage; protein YagM
RA 306,370 C→T G150S (GGC→AGC)  ecpD ← fimbrial adhesin EcpD
RA 309,218 Δ1 bp coding (115/2526 nt) ecpC ← putative fimbrial usher protein EcpC
RA 310,270 G→A G134G (GGC→GGT ecpA ← common pilus major subunit
RA 349,262 C→T A194V (GCC→GTC)  prpB → 2‑methylisocitrate lyase
RA 353,381 G→A V226M (GTG→ATG)  prpE → propionyl‑CoA synthetase
RA 359,674 G→A G62G (GGG→GGA cynS → cyanase
RA 361,727 (T)6→7 coding (134/612 nt) lacA ← galactoside O‑acetyltransferase
RA 366,168 A→T D46E (GAT→GAA lacZ ← beta‑galactosidase
RA 385,106 T→C intergenic (‑490/‑126) yaiS ← / → tauA putative deacetylase YaiS/taurine ABC transporter periplasmic binding protein
RA 397,213 A→T Q192L (CAG→CTG)  sbmA → peptide antibiotic/peptide nucleic acid transporter
RA 402,171 T→A L142Q (CTG→CAG)  phoA → alkaline phosphatase
RA 435,879 T→A D82E (GAT→GAA thiL → thiamine monophosphate kinase
RA 447,488 T→C N73S (AAT→AGT)  cyoE ← heme O synthase
RA 448,092 Δ1 bp coding (569/615 nt) cyoC ← cytochrome bo3 ubiquinol oxidase subunit 3
RA 453,654 C→G D172H (GAT→CAT)  yajG ← putative lipoprotein YajG
RA 458,055 A→T A210A (GCA→GCT clpX → ATP‑dependent Clp protease ATP‑binding subunit ClpX
RA 475,050 G→A Q38* (CAG→TAG)  tesB ← acyl‑CoA thioesterase II
RA 475,469 C→T P31S (CCG→TCG)  ybaY → PF09619 family lipoprotein YbaY
RA 511,329 G→T C17* (TGC→TGA
C17* (TGC→TGA
copA ←
copA ←
Cu(+) exporting P‑type ATPase
Cu(+) exporting P‑type ATPase
RA 514,719 C→T W47* (TGG→TGA ybbJ ← NfeD‑like family protein
RA 522,992 C→T intergenic (+162/‑269) ybbP → / → rhsD putative ABC transporter membrane subunit YbbP/protein RhsD
RA 548,605 G→A E83E (GAG→GAA ylbE → DUF1116 domain‑containing protein YlbE
RA 551,066 G→T A210S (GCC→TCC)  ybcF → putative carbamate kinase
RA 578,636 A→T Y86* (TAT→TAA borD ← DLP12 prophage; prophage lipoprotein BorD
RA 584,131 C→A R151R (CGG→AGG)  appY → DNA‑binding transcriptional activator AppY
RA 585,822 (T)7→8 intergenic (‑189/+325) ompT ← / ← envY outer membrane protease VII (outer membrane protein 3b)/DNA‑binding transcriptional activator EnvY
RA 589,509 G→A A482A (GCC→GCT nfrA ← bacteriophage N4 receptor, outer membrane protein
RA 602,184 G→A E76K (GAA→AAA)  pheP → phenylalanine:H(+) symporter PheP
RA 616,523 G→A A789A (GCG→GCA entF → apo‑serine activating enzyme
RA 649,472 G→A Q34* (CAA→TAA)  citF ← citrate lyase alpha subunit
RA 651,373 G→A R162C (CGT→TGT)  citC ← citrate lyase synthetase
RA 693,279 G→A R34C (CGC→TGC)  ybeZ ← PhoH‑like protein
RA 713,939 C→T L128L (CTG→TTG)  pgm → phosphoglucomutase
RA 714,976 G→A L473L (CTG→CTA pgm → phosphoglucomutase
RA 720,905 A→G intergenic (+65/+151) ybfK → / ← kdpE uncharacterized protein YbfK/DNA‑binding transcriptional activator KdpE
RA 734,251 C→T pseudogene (32/1434 nt) ybfO → conserved protein, rhs‑like YbfO
RA 742,374 C→T T61T (ACG→ACA dtpD ← dipeptide:H(+) symporter DtpD
RA 747,445 G→T P109T (CCT→ACT)  abrB ← putative regulator
RA 807,445 C→A A155A (GCG→GCT ybhB ← putative kinase inhibitor
RA 808,820 G→T G146G (GGC→GGA bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 812,748 C→T A160V (GCA→GTA)  bioD → dethiobiotin synthetase
RA 862,428 A→T L60Q (CTG→CAG)  ybiW ← putative pyruvate formate lyase
RA 869,465 T→C L19L (TTG→CTG)  gsiB → glutathione ABC transporter periplasmic binding protein
RA 871,477 C→T Q171* (CAG→TAG)  gsiC → glutathione ABC transporter membrane subunit GsiC
RA 895,153 G→A G55S (GGC→AGC)  potG → putrescine ABC transporter ATP binding subunit
RA 916,809 C→A R113S (CGT→AGT)  ybjD → DUF2813 domain‑containing protein YbjD
RA 937,762 G→A G131S (GGC→AGC)  lolA → outer membrane lipoprotein carrier protein
RA 960,227 A→G intergenic (+132/‑37) aroA → / → ycaL 3‑phosphoshikimate 1‑carboxyvinyltransferase/periplasmic protease YcaL
RA 974,632 C→A P105Q (CCG→CAG)  mukF → chromosome partitioning protein MukF
RA 982,951 C→T intergenic (+57/‑124) ldtD → / → ycbK L,D‑transpeptidase LdtD/DUF882 domain‑containing protein YcbK
RA 1,008,814 C→T A324V (GCG→GTG)  rlmL → fused 23S rRNA m(2)G2445 methyltransferase and 23S rRNA m(7)G2069 methyltransferase
RA 1,020,384 (A)7→8 intergenic (‑331/+26) ompA ← / ← sulA outer membrane porin A/cell division inhibitor SulA
RA 1,045,600 A→G I68I (ATT→ATC gfcE ← putative exopolysaccharide export lipoprotein GfcE
RA 1,047,811 C→T M45I (ATG→ATA gfcD ← putative lipoprotein GfcD
RA 1,069,647 A→G S65P (TCG→CCG)  rutG ← pyrimidine:H(+) symporter
RA 1,110,221 A→G H296R (CAT→CGT)  opgG → osmoregulated periplasmic glucans (OPGs) biosynthesis protein G
RA 1,113,489 G→A intergenic (+83/‑90) opgH → / → yceK osmoregulated periplasmic glucans (OPGs) biosynthesis protein H/DUF1375 domain‑containing lipoprotein YceK
RA 1,113,730 T→A I51N (ATC→AAC)  yceK → DUF1375 domain‑containing lipoprotein YceK
RA 1,144,461 T→A intergenic (‑94/‑41) rne ← / → yceQ ribonuclease E/DUF2655 domain‑containing protein YceQ
RA 1,166,055 C→T intergenic (+370/‑30) ycfP → / → ndh UPF0227 protein YcfP/NADH:quinone oxidoreductase II
RA 1,183,061 G→A A187V (GCG→GTG)  potC ← spermidine preferential ABC transporter membrane subunit PotC
RA 1,183,867 G→A T193M (ACG→ATG)  potB ← spermidine preferential ABC transporter membrane subunit PotB
RA 1,184,272 G→T S58* (TCG→TAG)  potB ← spermidine preferential ABC transporter membrane subunit PotB
RA 1,199,503 (A)5→4 coding (809/894 nt) lit → e14 prophage; cell death peptidase Lit
RA 1,238,385 T→G L272R (CTG→CGG)  dadA → D‑amino acid dehydrogenase
RA 1,239,692 T→C Y272H (TAC→CAC)  dadX → alanine racemase 2
RA 1,257,004 A→T I270N (ATC→AAC)  ychF ← redox‑responsive ATPase YchF
RA 1,290,291 A→C Q17P (CAG→CCG)  rssB → regulator of RpoS
RA 1,301,472 C→T V97V (GTC→GTT oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,304,336 +T coding (503/909 nt) oppC → murein tripeptide ABC transporter/oligopeptide ABC transporter inner membrane subunit OppC
RA 1,311,270 T→C V61A (GTT→GCT)  tonB → Ton complex subunit TonB
RA 1,319,991 G→A P465L (CCG→CTG)  trpD ← anthranilate synthase subunit TrpD
RA 1,321,337 G→A N16N (AAC→AAT trpD ← anthranilate synthase subunit TrpD
RA 1,353,437 A→T T64T (ACT→ACA sapD ← putrescine ABC exporter ATP binding protein SapD
RA 1,367,811 G→A I34I (ATC→ATT pspF ← DNA‑binding transcriptional dual regulator PspF
RA 1,373,097 G→A V397M (GTG→ATG)  ycjN → putative ABC transporter periplasmic binding protein YcjN
RA 1,411,109 A→T D280E (GAT→GAA ttcA ← tRNA cytosine(32) 2‑sulfurtransferase TtcA
RA 1,452,220 G→A P475S (CCT→TCT)  paaZ ← oxepin‑CoA hydrolase/3‑oxo‑5,6‑dehydrosuberyl‑CoA semialdehyde dehydrogenase
RA 1,475,586 C→T P148L (CCG→CTG)  ydbD → DUF2773 domain‑containing protein YdbD
RA 1,478,850 C→T L209L (CTG→TTG)  ynbB → putative CDP‑diglyceride synthase
RA 1,483,915 G→A L285L (CTG→CTA hrpA → ATP‑dependent RNA helicase HrpA
RA 1,506,257 C→T A29V (GCG→GTG)  sutR → DNA‑binding transcriptional dual regulator SutR
RA 1,508,746 G→A intergenic (+4/+88) rlhA → / ← yncJ 23S rRNA 5‑hydroxycytidine C2501 synthase/protein YncJ
RA 1,513,132 G→A V106M (GTG→ATG)  ydcT → putative ABC transporter ATP‑binding protein YdcT
RA 1,527,350 G→A intergenic (+198/‑540) yncH → / → rhsE DUF5445 domain‑containing protein YncH/RhsE protein in rhs element
RA 1,539,530 A→G S1021P (TCC→CCC)  narZ ← nitrate reductase Z subunit alpha
RA 1,542,678 T→C Q461Q (CAA→CAG narU ← nitrate/nitrite transporter NarU
RA 1,549,287 C→A S629S (TCC→TCA fdnG → formate dehydrogenase N subunit alpha
RA 1,582,328 T→C D66G (GAT→GGT)  ydeN ← putative sulfatase
RA 1,610,848 C→T intergenic (‑168/+59) uxaB ← / ← dgcF tagaturonate reductase/putative diguanylate cyclase DgcF
RA 1,612,952 G→A S100S (TCC→TCT glsB ← glutaminase 2
RA 1,615,877 A→T T39S (ACA→TCA)  yneK → protein YneK
RA 1,621,770 A→G I147V (ATC→GTC)  ydeE → dipeptide exporter
RA 1,625,496 A→G F629L (TTT→CTT)  dcp ← peptidyl‑dipeptidase Dcp
RA 1,660,717 G→A A54A (GCG→GCA ynfF → putative selenate reductase YnfF
RA 1,662,456 T→A L634* (TTG→TAG)  ynfF → putative selenate reductase YnfF
RA 1,671,696 T→C intergenic (+12/‑81) asr → / → ydgU acid shock protein/uncharacterized protein YdgU
RA 1,706,877 A→T D177V (GAT→GTT)  rsxB → SoxR [2Fe‑2S] reducing system protein RsxB
RA 1,722,167 C→T A16V (GCC→GTC)  ydhK → putative transporter YdhK
RA 1,728,574 C→T A76A (GCC→GCT rnt → RNase T
RA 1,730,699 C→T A538V (GCT→GTT)  lhr → putative ATP‑dependent helicase Lhr
RA 1,738,556 A→G H238R (CAT→CGT)  purR → DNA‑binding transcriptional repressor PurR
RA 1,748,585 T→A R485R (CGT→CGA ydhS → FAD/NAD(P) binding domain‑containing protein YdhS
RA 1,767,394 T→C H431R (CAC→CGC)  ydiJ ← putative FAD‑linked oxidoreductase
RA 1,787,398 (T)5→6 intergenic (‑286/‑47) ppsA ← / → ppsR phosphoenolpyruvate synthetase/posphoenolpyruvate synthetase regulatory protein
RA 1,789,895 C→T L450L (TTG→TTA ydiU ← UPF0061 family protein YdiU
RA 1,810,695 T→C M162T (ATG→ACG)  ydjM → putative inner membrane protein regulated by LexA
RA 1,831,779 C→T A68A (GCG→GCA astC ← succinylornithine transaminase
RA 1,852,829 C→T R384H (CGC→CAC)  ydjE ← putative transporter YdjE
RA 1,855,607 A→T L122* (TTG→TAG)  ydjG ← NADH‑dependent methylglyoxal reductase
RA 1,870,272 T→C intergenic (+34/‑113) yeaH → / → cdgI DUF444 domain‑containing protein YeaH/putative c‑di‑GMP binding protein CdgI
RA 1,875,384 G→A Q65* (CAA→TAA)  nimR ← DNA‑binding transcriptional repressor NimR
RA 1,882,216 A→T Q102L (CAG→CTG)  dmlA → D‑malate/3‑isopropylmalate dehydrogenase (decarboxylating)
RA 1,900,559 C→A E343* (GAA→TAA)  yoaE ← putative inner membrane protein
RA 1,907,906 C→T intergenic (‑315/+355) yobF ← / ← yebO DUF2527 domain‑containing protein YobF/uncharacterized protein YebO
RA 1,935,168 C→T D383N (GAT→AAT)  zwf ← NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
RA 1,942,614 A→G intergenic (‑31/‑48) znuA ← / → znuC Zn(2(+)) ABC transporter periplasmic binding protein/Zn(2(+)) ABC transporter ATP binding subunit
RA 1,990,813 T→A V378E (GTG→GAG)  tyrP → tyrosine:H(+) symporter
RA 2,055,073 G→A G5S (GGC→AGC)  amn → AMP nucleosidase
RA 2,066,740 A→G G194G (GGT→GGC insH‑6 ← CP4‑44 prophage; IS5 transposase and trans‑activator
RA 2,082,090 T→C N153S (AAC→AGC)  dacD ← D‑alanyl‑D‑alanine carboxypeptidase DacD
RA 2,101,774 T→C intergenic (‑30/+121) wbbL ← / ← insH‑7 interrupted rhamnosyltransferase WbbL/IS5 transposase and trans‑activator
RA 2,103,170 T→C pseudogene (220/446 nt) wbbL ← interrupted rhamnosyltransferase WbbL
RA 2,124,626 G→T A374E (GCG→GAG)  wcaI ← putative colanic biosynthesis glycosyl transferase
RA 2,128,919 T→C L244L (TTA→TTG wcaE ← putative colanic acid biosynthesis glycosyl transferase
RA 2,142,743 G→A P69L (CCC→CTC)  udk ← uridine/cytidine kinase
RA 2,153,777 T→C noncoding (132/136 nt) sibB → small RNA SibB
RA 2,158,945 T→C V187A (GTC→GCC)  mdtC → multidrug efflux pump RND permease subunit MdtC
RA 2,182,770 C→A A4A (GCG→GCT yegW ← putative DNA‑binding transcriptional regulator YegW
RA 2,185,882 A→T intergenic (‑86/‑35) rcnR ← / → rcnA DNA‑binding transcriptional repressor RcnR/Ni(2(+))/Co(2(+)) exporter
RA 2,192,383 A→G intergenic (‑161/+132) yehD ← / ← yehE putative fimbrial protein YehD/DUF2574 domain‑containing protein YehE
RA 2,200,118 C→T intergenic (+2820/‑161) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI
RA 2,202,498 A→T A740A (GCA→GCT yehI → DUF4132 domain‑containing protein YehI
RA 2,204,258 T→C L96P (CTA→CCA)  yehK → uncharacterized protein YehK
RA 2,222,467 G→A D95N (GAT→AAT)  dld → D‑lactate dehydrogenase
RA 2,234,328 G→A G99D (GGC→GAC)  preT → NAD‑dependent dihydropyrimidine dehydrogenase subunit PreT
RA 2,240,203 T→A Q49L (CAA→CTA)  mglB ← D‑galactose/methyl‑galactoside ABC transporter periplasmic binding protein
RA 2,243,335 A→G T106T (ACT→ACC folE ← GTP cyclohydrolase 1
RA 2,248,232 T→A N101Y (AAC→TAC)  lysP ← lysine:H(+) symporter
RA 2,259,563 T→C intergenic (‑267/+156) psuK ← / ← fruA putative pseudouridine kinase/fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,276,072 T→C F267S (TTT→TCT)  yejE → putative oligopeptide ABC transporter membrane subunit YejE
RA 2,295,698 C→T G208D (GGC→GAC)  ccmC ← heme trafficking system membrane protein CcmC
RA 2,313,055 A→G intergenic (‑306/‑29) ompC ← / → micF outer membrane porin C/small regulatory RNA MicF
RA 2,356,245 C→T S242L (TCA→TTA)  glpC → anaerobic glycerol‑3‑phosphate dehydrogenase subunit C
RA 2,360,002 C→T E51K (GAA→AAA)  yfaV ← putative transporter YfaV
RA 2,365,594 C→T L9L (TTG→TTA ais ← putative lipopolysaccharide core heptose(II)‑phosphate phosphatase
RA 2,371,842 T→A L312* (TTG→TAG)  arnT → lipid IVA 4‑amino‑4‑deoxy‑L‑arabinosyltransferase
RA 2,374,272 T→C L244L (TTA→TTG menE ← o‑succinylbenzoate‑‑CoA ligase
RA 2,387,378 A→G S149G (AGC→GGC)  yfbM → DUF1877 domain‑containing protein YfbM
RA 2,395,206 A→T V47E (GTG→GAG)  nuoK ← NADH:quinone oxidoreductase subunit K
RA 2,399,514 T→C K218E (AAA→GAA)  nuoG ← NADH:quinone oxidoreductase subunit G
RA 2,426,400 G→T S130* (TCG→TAG)  hisJ ← histidine ABC transporter periplasmic binding protein
RA 2,428,535 Δ1 bp coding (92/570 nt) ubiX ← flavin prenyltransferase
RA 2,460,346 T→A K42* (AAG→TAG)  fadI ← 3‑ketoacyl‑CoA thiolase FadI
RA 2,461,596 C→T N97N (AAC→AAT fadL → long‑chain fatty acid outer membrane channel/bacteriophage T2 receptor
RA 2,465,985 A→C T229P (ACC→CCC)  yfdC → inner membrane protein YfdC
RA 2,497,234 C→A A354A (GCG→GCT alaC ← glutamate‑‑pyruvate aminotransferase AlaC
RA 2,513,581 C→T S180S (TCC→TCT nupC → nucleoside:H(+) symporter NupC
RA 2,522,542 C→T intergenic (‑65/+187) xapR ← / ← xapB DNA‑binding transcriptional activator XapR/xanthosine:H(+) symporter XapB
RA 2,539,484 T→A N34Y (AAT→TAT)  cysM ← cysteine synthase B
RA 2,555,565 G→T A55E (GCG→GAG)  eutK ← putative structural protein, ethanolamine utilization microcompartment
RA 2,557,470 A→G Y404H (TAC→CAC)  eutB ← ethanolamine ammonia‑lyase subunit alpha
RA 2,562,119 C→T G3G (GGC→GGT yffO → CPZ‑55 prophage; uncharacterized protein YffO
RA 2,574,860 A→T C48* (TGT→TGA eutQ ← putative ethanolamine utilization protein EutQ
RA 2,580,405 A→G E257G (GAA→GGA)  tktB → transketolase 2
RA 2,590,292 A→G M900V (ATG→GTG)  acrD → multidrug efflux pump RND permease AcrD
RA 2,592,993 C→T intergenic (+31/+79) ypfN → / ← ypfH UPF0370 protein YpfN/esterase YpfH
RA 2,634,630 (A)6→7 coding (399/1371 nt) xseA → exodeoxyribonuclease VII subunit XseA
RA 2,638,485 A→G I56I (ATT→ATC bamB ← outer membrane protein assembly factor BamB
RA 2,647,072 T→C D85G (GAT→GGT)  pbpC ← peptidoglycan glycosyltransferase PbpC
RA 2,653,478 C→A intergenic (+139/‑377) sseA → / → ryfA 3‑mercaptopyruvate sulfurtransferase/small regulatory RNA RyfA
RA 2,659,676 A→T F71I (TTT→ATT)  iscA ← iron‑sulfur cluster insertion protein IscA
RA 2,677,674 C→A D230Y (GAT→TAT)  yphE ← putative ABC transporter ATP‑binding protein YphE
RA 2,695,642 A→G intergenic (‑99/‑159) purL ← / → mltF phosphoribosylformylglycinamide synthetase/membrane‑bound lytic murein transglycosylase F
RA 2,712,311 G→A L152L (CTG→TTG)  yfiC ← tRNA m(6)A37 methyltransferase
JC JC 2,737,118 IS1 (+) +8 bp intergenic (+235/‑29) bamD → / → raiA outer membrane protein assembly factor BamD/stationary phase translation inhibitor and ribosome stability factor
RA 2,750,090 C→T intergenic (+30/+25) yfjD → / ← grpE putative inner membrane protein/nucleotide exchange factor GrpE
RA 2,751,434 T→C S202P (TCT→CCT)  nadK → NAD kinase
RA 2,773,721 T→C F135S (TTT→TCT)  yfjW → CP4‑57 prophage; uncharacterized protein YfjW
RA 2,779,727 A→G N1000N (AAT→AAC ypjA ← adhesin‑like autotransporter YpjA
RA 2,782,393 (C)5→4 coding (334/4581 nt) ypjA ← adhesin‑like autotransporter YpjA
RA 2,782,394 G→A D111D (GAC→GAT ypjA ← adhesin‑like autotransporter YpjA
RA 2,827,666 T→A V102E (GTG→GAG)  srlB → sorbitol‑specific PTS enzyme IIA component
RA 2,832,654 T→A V60E (GTG→GAG)  norV → anaerobic nitric oxide reductase flavorubredoxin
RA 2,844,396 G→A V119V (GTC→GTT hycF ← formate hydrogenlyase subunit HycF
RA 2,860,308 T→C F183F (TTT→TTC pphB → phosphoprotein phosphatase 2
RA 2,894,763 T→A L190I (TTA→ATA)  ygcP → putative anti‑terminator regulatory protein
RA 2,900,805 T→A F72I (TTT→ATT)  yqcE → putative transport protein YqcE
RA 2,906,427 A→G I239M (ATA→ATG ygcG → protein YgcG
RA 2,907,489 C→T M151I (ATG→ATA eno ← enolase
RA 2,932,902 C→A D38Y (GAT→TAT)  fucO ← L‑1,2‑propanediol oxidoreductase
RA 2,937,860 C→T A131A (GCC→GCT fucK → L‑fuculokinase
RA 2,958,495 A→G Y130Y (TAT→TAC ptrA ← protease 3
RA 2,960,676 C→T E585K (GAA→AAA)  recC ← exodeoxyribonuclease V subunit RecC
RA 2,975,617 A→G I133T (ATT→ACT)  aas ← acyltransferase
RA 3,008,343 T→C V278V (GTT→GTC ygeX → 2,3‑diaminopropionate ammonia‑lyase
RA 3,016,863 C→A I268I (ATC→ATA ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,017,496 A→T E479D (GAA→GAT ygfK → putative oxidoreductase, Fe‑S subunit
RA 3,046,867 G→A G725G (GGC→GGT gcvP ← glycine decarboxylase
RA 3,073,292 C→A V134F (GTT→TTT)  epd ← D‑erythrose‑4‑phosphate dehydrogenase
RA 3,077,659 C→T A401T (GCA→ACA)  cmtA ← mannitol‑specific PTS enzyme IICB component CmtA
RA 3,082,793 G→A intergenic (+122/+84) loiP → / ← speB metalloprotease LoiP/agmatinase
RA 3,088,585 C→T A101V (GCT→GTT)  galP → galactose:H(+) symporter
RA 3,094,484 G→A L199L (CTC→CTT yggR ← Type II/IV secretion system family protein
RA 3,113,514 +A intergenic (‑37/+29) yghG ← / ← pppA lipoprotein YghG/prepilin peptidase
RA 3,121,875 T→C N644S (AAC→AGC)  glcB ← malate synthase G
RA 3,142,898 G→A A31V (GCA→GTA)  hybC ← hydrogenase 2 large subunit
RA 3,149,148 T→C K108K (AAA→AAG yqhA ← uncharacterized protein YqhA
RA 3,152,036 C→T intergenic (‑52/‑200) exbB ← / → metC Ton complex subunit ExbB/cystathionine beta‑lyase/L‑cysteine desulfhydrase
RA 3,152,994 C→T S253S (AGC→AGT metC → cystathionine beta‑lyase/L‑cysteine desulfhydrase
RA 3,187,691 G→A pseudogene (260/2434 nt) yqiG → putative outer membrane usher protein YqiG
RA 3,201,749 G→A L181L (CTG→CTA ygiM → putative signal transduction protein (SH3 domain)
RA 3,223,904 C→T Y424Y (TAC→TAT ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,225,566 C→T S978S (AGC→AGT ebgA → evolved beta‑D‑galactosidase subunit alpha
RA 3,290,288 A→G Y492C (TAT→TGT)  yraJ → putative fimbrial usher protein YraJ
RA 3,332,210 C→A V176L (GTG→TTG)  yhbE ← inner membrane protein YhbE
RA 3,360,724 T→C intergenic (+108/‑452) gltD → / → gltF glutamate synthase subunit GltD/periplasmic protein GltF
RA 3,368,665 T→G L280R (CTG→CGG)  yhcG → DUF1016 domain‑containing protein YhcG
RA 3,374,775 C→T intergenic (‑286/‑94) nanR ← / → dcuD DNA‑binding transcriptional dual regulator NanR/putative transporter DcuD
RA 3,374,944 G→A V26M (GTG→ATG)  dcuD → putative transporter DcuD
RA 3,397,596 A→T F67Y (TTT→TAT)  rng ← RNase G
RA 3,403,864 C→T A127A (GCC→GCT yhdH → acrylyl‑CoA reductase
RA 3,432,299 +C coding (166/474 nt) smg ← DUF494 domain‑containing protein Smg
RA 3,435,342 G→A E46K (GAG→AAG)  rsmB → 16S rRNA m(5)C967 methyltransferase
RA 3,447,485 A→G F95L (TTC→CTC)  rplX ← 50S ribosomal subunit protein L24
RA 3,454,025 T→A S458S (TCA→TCT gspA ← Type II secretion system protein GspA
RA 3,471,692 G→T A608E (GCG→GAG)  fusA ← elongation factor G
RA 3,489,824 T→A A119A (GCA→GCT argD ← N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase
RA 3,497,005 G→A M1M (ATG→ATA) † nirC → nitrite transporter NirC
RA 3,499,007 C→T L394L (CTG→TTG)  cysG → siroheme synthase
RA 3,543,611 C→T L149F (CTT→TTT)  rpnA → recombination‑promoting nuclease RpnA
RA 3,547,765 G→T E403* (GAG→TAG)  gntT → high‑affinity gluconate transporter
RA 3,549,669 C→T P134P (CCG→CCA malQ ← 4‑alpha‑glucanotransferase
RA 3,589,355 C→T V225M (GTG→ATG)  ugpE ← sn‑glycerol 3‑phosphate ABC transporter membrane subunit UgpE
RA 3,607,423 G→T A325S (GCC→TCC)  zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,608,490 C→T A680A (GCC→GCT zntA → Zn(2(+))/Cd(2(+))/Pb(2(+)) exporting P‑type ATPase
RA 3,611,739 T→A intergenic (‑6/‑126) yhhS ← / → yhhT putative transporter YhhS/putative transporter YhhT
RA 3,633,702 C→T pseudogene (757/1607 nt) yhiL ← putative uncharacterized protein YhiL
RA 3,634,444 G→A pseudogene (15/1607 nt) yhiL ← putative uncharacterized protein YhiL
RA 3,649,690 A→T A252A (GCA→GCT arsB → arsenite/antimonite:H(+) antiporter
RA 3,657,601 G→A intergenic (+34/+385) hdeD → / ← arrS acid‑resistance membrane protein/small regulatory RNA ArrS
RA 3,665,991 A→G intergenic (‑181/+189) gadX ← / ← gadA DNA‑binding transcriptional dual regulator GadX/glutamate decarboxylase A
RA 3,672,739 Δ1 bp coding (326/900 nt) yhjC → putative DNA‑binding transcriptional regulator YhjC
RA 3,704,055 G→A A236V (GCT→GTT)  dppC ← dipeptide ABC transporter membrane subunit DppC
RA 3,734,789 T→A L357Q (CTG→CAG)  xylH → xylose ABC transporter membrane subunit
RA 3,740,658 C→A I318I (ATC→ATA avtA → valine‑‑pyruvate aminotransferase
RA 3,769,837 (T)7→8 intergenic (+157/+406) yibV → / ← yibH PF15596 family protein YibV/inner membrane protein YibH
RA 3,807,326 T→C D258G (GAT→GGT)  waaQ ← lipopolysaccharide core heptosyltransferase 3
RA 3,820,029 A→G V381A (GTG→GCG)  ligB ← DNA ligase B
RA 3,834,019 C→T V173V (GTG→GTA yicI ← alpha‑D‑xyloside xylohydrolase
RA 3,864,243 C→T I115I (ATC→ATT yidP → putative DNA‑binding transcriptional regulator YidP
RA 3,904,521 C→T A359A (GCG→GCA bglF ← beta‑glucoside specific PTS enzyme II/BglG kinase/BglG phosphatase
RA 3,921,309 A→T I248N (ATC→AAC)  atpB ← ATP synthase Fo complex subunit a
RA 3,924,204 C→T R477H (CGT→CAT)  mnmG ← 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
RA 3,969,234 G→A M68I (ATG→ATA wzzE → enterobacterial common antigen polysaccharide co‑polymerase
RA 3,991,035 C→T intergenic (‑269/‑118) hemC ← / → cyaA hydroxymethylbilane synthase/adenylate cyclase
RA 4,020,179 C→T R186C (CGT→TGT) 
noncoding (202/252 nt)
ubiJ →
esrE →
ubiquinone biosynthesis accessory factor UbiJ
small RNA EsrE
RA 4,033,383 T→A L80Q (CTG→CAG)  trkH → K(+) transporter TrkH
RA 4,041,630 A→G W124R (TGG→CGG)  mobA ← molybdenum cofactor guanylyltransferase
RA 4,053,227 C→T H395Y (CAC→TAC)  hemN → coproporphyrinogen III dehydrogenase
RA 4,053,455 A→G intergenic (+37/+192) hemN → / ← yshB coproporphyrinogen III dehydrogenase/uncharacterized protein YshB
RA 4,081,536 A→C F109V (TTC→GTC)  fdoI ← formate dehydrogenase O subunit gamma
RA 4,093,668 (T)5→4 coding (605/825 nt) rhaD ← rhamnulose‑1‑phosphate aldolase
RA 4,104,429 C→T A183T (GCC→ACC)  cpxA ← sensory histidine kinase CpxA
RA 4,139,431 A→G L93P (CTG→CCG)  fsaB ← fructose‑6‑phosphate aldolase 2
RA 4,144,522 G→A M176I (ATG→ATA pflD → putative formate acetyltransferase 2
RA 4,156,086 G→A S25N (AGC→AAC)  argB → acetylglutamate kinase
RA 4,177,755 C→T P5S (CCT→TCT)  nusG → transcription termination factor NusG
RA 4,182,820 C→T H526Y (CAC→TAC)  rpoB → RNA polymerase subunit beta
RA 4,183,748 A→G E835G (GAA→GGA)  rpoB → RNA polymerase subunit beta
RA 4,184,140 A→G I966V (ATC→GTC)  rpoB → RNA polymerase subunit beta
RA 4,224,682 T→G D285E (GAT→GAG metH → cobalamin‑dependent methionine synthase
RA 4,276,458 A→G M330V (ATG→GTG)  pdeC → c‑di‑GMP phosphodiesterase PdeC
RA 4,293,531 G→A V123V (GTG→GTA nrfF → putative formate‑dependent nitrite reductase complex subunit NrfF
RA 4,316,957 Δ1 bp coding (262/1137 nt) phnM ← RPnTP hydrolase
RA 4,319,653 G→A F337F (TTC→TTT phnI ← carbon‑phosphorus lyase core complex subunit PhnI
RA 4,323,424 G→T T310N (ACC→AAC)  phnD ← phosphonate ABC transporter periplasmic binding protein
RA 4,324,004 G→A P117S (CCG→TCG)  phnD ← phosphonate ABC transporter periplasmic binding protein
RA 4,333,287 T→C N222D (AAC→GAC)  basR ← DNA‑binding transcriptional dual regulator BasR
RA 4,333,655 A→T L99Q (CTG→CAG)  basR ← DNA‑binding transcriptional dual regulator BasR
RA 4,391,914 T→C I104T (ATT→ACT)  orn → oligoribonuclease
RA 4,410,422 T→C V97A (GTT→GCT)  yjfI → conserved protein YjfI
RA 4,418,094 G→A I120I (ATC→ATT ulaR ← DNA‑binding transcriptional repressor UlaR
RA 4,460,132 T→C H78R (CAT→CGT)  nrdG ← anaerobic ribonucleoside‑triphosphate reductase activating protein
RA 4,479,585 C→T intergenic (‑48/‑145) yjgM ← / → yjgN putative acetyltransferase YjgM/conserved inner membrane protein YjgN
RA 4,480,873 G→A G382S (GGC→AGC)  yjgN → conserved inner membrane protein YjgN
RA 4,504,097 C→T R14W (CGG→TGG)  yjhB → putative sialic acid transporter
RA 4,505,423 G→A C46Y (TGT→TAT)  yjhC → KpLE2 phage‑like element; putative oxidoreductase YjhC
RA 4,538,483 T→A K95* (AAA→TAA)  nanM ← N‑acetylneuraminate mutarotase
RA 4,545,445 T→C M117T (ATG→ACG)  fimD → type I fimbriae usher protein
RA 4,551,712 G→A G26D (GGC→GAC)  uxuA → D‑mannonate dehydratase
RA 4,559,565 T→A I146F (ATC→TTC)  yjiG ← Gate family protein YjiG
RA 4,559,878 C→T A41A (GCG→GCA yjiG ← Gate family protein YjiG
RA 4,579,647 C→A G65V (GGC→GTC)  symE ← toxic protein SymE
RA 4,590,284 G→A F332F (TTC→TTT btsT ← pyruvate:H(+) symporter
RA 4,603,273 C→T intergenic (‑415/‑204) yjjP ← / → yjjQ putative succinate exporter YjjP/DNA‑binding transcriptional repressor YjjQ
RA 4,635,821 G→A G101S (GGC→AGC)  creA → PF05981 family protein CreA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423723–3424234 3424585–3424238 5–863 5 [4] [4] 5 [rrfD]–[rrlD] [rrfD],[rrlD]
* * ÷ NC_000913 3751936 3752011 76 5 [4] [4] 6 [yiaU] [yiaU]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =6 (0.190)7 (0.270) 7/128 1.9 58.9% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 5 (0.200)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12078056 (0.190)15 (0.590) 14/128 0.8 75.4% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 5 (0.200)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment