breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
MC JC 1,299,499 Δ1,199 bp intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,755,429 (T)5→4 intergenic (‑288/‑269) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,533,492 A→T intergenic (+112/‑272) cysK → / → ptsH cysteine synthase A, O‑acetylserine sulfhydrolase A subunit/phosphohistidinoprotein‑hexose phosphotransferase component of PTS system (Hpr)
RA 2,628,644 A→T C99S (TGT→AGT)  yfgF ← cyclic‑di‑GMP phosphodiesterase, anaerobic
JC 2,809,897 (TTCGCCATGTGTTGTATGG)1→2 coding (281/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 3,181,174 G→A G252S (GGC→AGC)  ygiC → ATP‑Grasp family ATPase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC JC 3,745,782 IS1 (–) +9 bp coding (979‑987/1278 nt) yiaN → 2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 4,132,227 C→A A798E (GCG→GAG)  metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,182,881 A→T E546V (GAA→GTA)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,510,524 C→A intergenic (+391/+166) yjhV → / ← fecE pseudogene, KpLE2 phage‑like element/ferric citrate ABC transporter ATPase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423812–3424501 3424501 1–690 53 [51] [52] 53 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =23 (0.190)104 (0.920) 78/536 0.2 86.8% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 10 (0.090)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780521 (0.170)109 (0.960) 75/536 0.2 88.0% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 10 (0.090)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related