Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,567 T→C 7.7% D1223D (GAT→GAC sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,5670TC 7.7% 500.0 / 8.3 183D1223D (GAT→GACsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base T (78/85);  new base C (13/3);  total (93/90)
Fisher's exact test for biased strand distribution p-value = 1.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

GATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTC  >  USA300TCH1516_ALE/618427‑618707
                                                                                                                                            |                                                                                                                                            
gATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACtcagat                                                                                                                                              <  1:202540/141‑1 (MQ=11)
    gcgactcagattcagacagcgactcagattcagacaGCGACTCAGGCGCAGATGGGGATGCATACTCAGAGAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACtcagattcag                                                                                                                                          <  1:1485022/141‑1 (MQ=17)
        ctcagattcagacagcgactcagattcagacagcgaCTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAg                                                                                                                                      <  4:689569/141‑1 (MQ=255)
           agattcagacagcgactcagattcagacagcgactcAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGa                                                                                                                                   <  1:901297/141‑1 (MQ=255)
            gattcagacagcgactcagattcagacagcgactcaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAc                                                                                                                                  <  3:209533/141‑1 (MQ=255)
            gattcagacagcgactcagattcagacagcgactcaGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAc                                                                                                                                  <  1:891067/141‑1 (MQ=255)
            gattcagacagcgactcagattcagacagcgactcaGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGAc                                                                                                                                  >  4:489764/1‑141 (MQ=255)
             actcagacagcgattcagattcagacagcgactcagaCTCGGATAGCGATTCAGATTCAGATAGCGATTCGGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACt                                                                                                                                 >  2:1213417/3‑141 (MQ=11)
                cagacagcgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAg                                                                                                                              >  2:1573561/1‑141 (MQ=255)
                       cgactcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                                       <  1:639775/141‑1 (MQ=255)
                       cgactcagattcagacagcgacacagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGa                                                                                                                       <  2:398300/141‑1 (MQ=255)
                          ctcagattcagacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAg                                                                                                                    <  3:543251/141‑1 (MQ=255)
                                   agccagcgagtcagaTTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                           <  1:1213543/138‑1 (MQ=255)
                                   agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                           <  3:355764/141‑1 (MQ=255)
                                   agacagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGa                                                                                                           >  1:347589/1‑141 (MQ=255)
                                     acagcgactcagaCTCAGATAGTGATTCAGACTCAGATAACGACTCAGATTCAGACAGCGACACAGACGCAGACAGCGCCTCAGACGTAGATAGTGATTCGCATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACt                                                                                                         >  1:1613537/1‑141 (MQ=14)
                                      cagcgactcagaCTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACCCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTc                                                                                                        <  1:1292414/141‑1 (MQ=255)
                                      cagcgactcagaCGCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTGAGATTCAGATAGCGACTCAGATTCGAATAGCGATTCAGACTc                                                                                                        >  1:435636/1‑141 (MQ=255)
                                            ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGAATCAGATAGCGACCGAGATTCAGAAAGCGATTCAGACTCAGATAg                                                                                                  >  2:231477/1‑141 (MQ=14)
                                            ctcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAg                                                                                                  <  2:688948/141‑1 (MQ=255)
                                             tcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACACAGACACAGACAGCGATTCAGACACAGATAgg                                                                                                 >  2:1546808/1‑140 (MQ=11)
                                             gcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCATACAGCGACTCAGACTCAGAGATTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGc                                                                                                 >  1:1569811/2‑141 (MQ=255)
                                              cagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCGGACAGCGATTCAGACCCAGATAGCg                                                                                                >  2:1000536/1‑141 (MQ=255)
                                               agcctcagaTACTGCTTCAGACTCCGATAGAGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGACAGCGACTCAGATTCGGACAGCGATTCAGGCTCAGATAGCGa                                                                                               <  2:1654133/138‑1 (MQ=14)
                                                gactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                              >  4:456746/1‑141 (MQ=255)
                                                gactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                              <  1:636382/141‑1 (MQ=255)
                                                gactcagaTAGCGATCCAGACTCAGATAGCGGCTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGAc                                                                                              <  2:844641/141‑1 (MQ=255)
                                                 actcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACt                                                                                             >  1:1200418/1‑141 (MQ=255)
                                                  ctcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGATTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtc                                                                                            <  1:879913/141‑1 (MQ=255)
                                                     agaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcaga                                                                                         <  1:1560668/141‑1 (MQ=255)
                                                      gaTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAACGACTCGGACGCAGATAGCGACTCAGACCCAGATAGCGACgcagat                                                                                        >  2:456167/1‑141 (MQ=11)
                                                      gaTAGTGATTCAGACTCAGATAGCGACTCAGAATCAGAAGGCGGCTGAGACGCGGACAGGGACTGGGACGCGGATAGTGATTCAGAGTCGGAGAGCGATTCAGAATCGGACAGCGATTCAGACTCAGATAGCGATtcagaa                                                                                        >  2:12793/1‑140 (MQ=2)
                                                       aTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGACAGCGACtcagact                                                                                       <  4:392365/141‑3 (MQ=255)
                                                       aTAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGAATCAGACTCAGATAGCGATtcaaacc                                                                                       >  2:1151980/1‑137 (MQ=255)
                                                         aGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattca                                                                                     >  3:317060/1‑141 (MQ=255)
                                                           tGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACtcagattcaga                                                                                   <  2:728236/141‑1 (MQ=255)
                                                              ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                >  1:514027/1‑141 (MQ=255)
                                                              ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  1:1037488/141‑1 (MQ=255)
                                                              ttCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                                                                <  2:438968/141‑1 (MQ=255)
                                                                  gACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAt                                                                            >  1:1177319/1‑141 (MQ=255)
                                                                   gcTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGAtt                                                                           >  4:407666/2‑141 (MQ=255)
                                                                   aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCg                                                                                                                                    >  4:147953/1‑84 (MQ=255)
                                                                   aCTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCg                                                                                                                                    <  3:147953/84‑1 (MQ=255)
                                                                        gATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGAc                                                                      <  4:119813/141‑1 (MQ=255)
                                                                        gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                      <  3:8920/141‑1 (MQ=255)
                                                                         aTAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACt                                                                     >  4:584193/1‑141 (MQ=255)
                                                                         aTAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACt                                                                     >  4:306614/1‑141 (MQ=255)
                                                                           aGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                             <  1:1173690/131‑1 (MQ=255)
                                                                            gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                  <  4:521973/141‑1 (MQ=255)
                                                                             cGACTCCGATTCCGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                 <  2:1511228/141‑1 (MQ=255)
                                                                              gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGa                                                                             >  2:1482777/1‑128 (MQ=255)
                                                                              gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAATCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAt                                                                >  2:1553867/1‑141 (MQ=255)
                                                                                cTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                              <  1:474699/141‑1 (MQ=255)
                                                                                  cAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTc                                                                          >  1:1383698/1‑127 (MQ=255)
                                                                                   aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGa                                                           >  1:1114303/1‑141 (MQ=255)
                                                                                      ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                        <  1:1391985/141‑1 (MQ=255)
                                                                                      ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                        <  1:724188/141‑1 (MQ=255)
                                                                                          gacagtgactcagactcagacagcgactcagactcaGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGAATCAGACACAGATAGCGAATCAGATCCAAATAGAGATACGCACGCAGATGGCGAGtcagag                                                    >  2:1367487/1‑140 (MQ=11)
                                                                                          gacagcgactcagactcagacagcgactcagactcaGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACtcagat                                                    >  2:813687/1‑141 (MQ=255)
                                                                                            cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                  >  1:1418071/1‑141 (MQ=255)
                                                                                            cagcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattc                                                  >  1:1079967/1‑141 (MQ=255)
                                                                                              gcgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcag                                                >  1:767872/1‑141 (MQ=255)
                                                                                               cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                               >  1:602814/1‑141 (MQ=255)
                                                                                                gactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcagat                                              <  1:1321121/141‑1 (MQ=255)
                                                                                                  ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                            <  3:671766/141‑1 (MQ=255)
                                                                                                  ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                            <  1:692929/141‑1 (MQ=255)
                                                                                                  ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAg                                            >  2:371072/1‑141 (MQ=255)
                                                                                                  ctcagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAAATACAGATac                                            >  2:50237/1‑140 (MQ=255)
                                                                                                    cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                          >  1:1240095/1‑141 (MQ=255)
                                                                                                    cagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTg                                          >  1:61038/1‑141 (MQ=255)
                                                                                                    cagactcagacagcgactcagacacagaTAGTGACCCAGACACAGATAGCGACTCAGATCCCGACAGCGAATCAGACTCAGATAGCGACCCAGATTCAGACAGCGATTCAGACTCAGATAGCGACCCAGAATCAGATAGTg                                          >  2:1099601/1‑141 (MQ=14)
                                                                                                     agactcagacagcgactcagactcagaTAGTGATTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                         <  3:426411/141‑1 (MQ=255)
                                                                                                      gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGAATCAGAAAGCGATTGGGACTCAGATAGAGACTCAGATTCAGATAGTGAt                                        >  2:226140/1‑141 (MQ=17)
                                                                                                         tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                     <  1:1425212/141‑1 (MQ=255)
                                                                                                         tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                     >  1:200686/1‑141 (MQ=255)
                                                                                                          cagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAg                                    >  2:746827/1‑141 (MQ=255)
                                                                                                           agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                   >  1:1008572/1‑141 (MQ=255)
                                                                                                           agacagcgactcagactcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                   <  2:1004730/141‑1 (MQ=255)
                                                                                                              ctgcgactgagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                <  2:28235/139‑1 (MQ=14)
                                                                                                              cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGATAGTGATTCAGACTc                                >  1:645511/1‑141 (MQ=255)
                                                                                                              cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                <  3:489764/141‑1 (MQ=255)
                                                                                                              cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                <  1:262482/141‑1 (MQ=255)
                                                                                                               agtgactcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                               <  2:1608011/138‑1 (MQ=255)
                                                                                                                 cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                             >  1:1000683/1‑141 (MQ=255)
                                                                                                                 cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                             <  3:456746/141‑1 (MQ=255)
                                                                                                                  gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGAt                            >  1:152813/1‑141 (MQ=255)
                                                                                                                  gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGAt                            >  1:91714/1‑141 (MQ=255)
                                                                                                                   actcagactcagaTAGTGACTCAGAATCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                           <  2:484879/141‑1 (MQ=255)
                                                                                                                    ctcagactcagaTGGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                          <  2:644100/141‑1 (MQ=255)
                                                                                                                    ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                          >  2:1414064/1‑141 (MQ=255)
                                                                                                                        gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGGCTCAGATAGCGAc                      >  3:102199/1‑141 (MQ=255)
                                                                                                                              gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGac                >  2:90425/1‑140 (MQ=255)
                                                                                                                                 aGTGACACAGATTCAGATACCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa             <  2:519944/141‑1 (MQ=255)
                                                                                                                                    gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc          <  1:388154/141‑1 (MQ=255)
                                                                                                                                      cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                       <  2:1064836/110‑1 (MQ=255)
                                                                                                                                        cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCg      >  1:1272094/1‑141 (MQ=255)
                                                                                                                                         agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa     >  1:1657629/1‑141 (MQ=255)
                                                                                                                                           attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGGGATTCAGACTCAGAGAGCGACTCAGATTCAGACAGCGACt   >  4:553818/1‑141 (MQ=255)
                                                                                                                                            ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc  >  1:1459692/1‑141 (MQ=255)
                                                                                                                                            ttcagatACCGACTCAGATTCAGAGAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc  <  2:1227427/141‑1 (MQ=14)
                                                                                                                                            |                                                                                                                                            
GATAGCGACTCAGATTCAGACAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTC  >  USA300TCH1516_ALE/618427‑618707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: