Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 618,636 G→A 5.5% S1246S (TCG→TCA sdrD → Serine‑aspartate repeat‑containing protein D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE618,6360GA 5.5% 486.1 / 5.7 179S1246S (TCG→TCAsdrDSerine‑aspartate repeat‑containing protein D
Reads supporting (aligned to +/- strand):  ref base G (92/71);  new base A (8/4);  total (100/79)
Fisher's exact test for biased strand distribution p-value = 5.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 181 total aligned reads displayed.

TCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCG  >  USA300TCH1516_ALE/618496‑618775
                                                                                                                                            |                                                                                                                                           
 cAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCgg                                                                                                                                            >  2:1597899/1‑141 (MQ=255)
  aGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                           <  1:979140/141‑1 (MQ=255)
   gATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGAc                                                                                                                                          <  3:8920/141‑1 (MQ=255)
    aTAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACt                                                                                                                                         >  4:306614/1‑141 (MQ=255)
       gCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAg                                                                                                                                      <  4:521973/141‑1 (MQ=255)
        cGACTCCGATTCCGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGa                                                                                                                                     <  2:1511228/141‑1 (MQ=255)
         gACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGAATCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGAt                                                                                                                                    >  2:1553867/1‑141 (MQ=255)
              aGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                               <  1:88240/141‑1 (MQ=255)
                 ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                            <  1:724188/141‑1 (MQ=255)
                 ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                            >  1:1124556/1‑141 (MQ=255)
                 ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                            <  1:1391985/141‑1 (MQ=255)
                 ttCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACtc                                                                                                                            >  4:213397/1‑141 (MQ=255)
                  tcagacagcgactcagactcagacagcgactcagacTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtca                                                                                                                           >  1:129413/1‑141 (MQ=255)
                    agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                                                                                                         >  1:1106594/1‑141 (MQ=255)
                    agacagcgactcagactcagacagcgactcagactcAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcaga                                                                                                                         <  2:525190/141‑1 (MQ=255)
                      acagcgactcagactcagacagcgactcagtctcagATAGTGACTCAGATGCAGAGAGCGACTCAGACTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagatt                                                                                                                       <  3:676299/141‑1 (MQ=255)
                          cgactcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACtcagattcaga                                                                                                                   >  1:602814/1‑141 (MQ=255)
                             ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                >  3:361421/1‑141 (MQ=255)
                             ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                >  1:1617569/1‑141 (MQ=255)
                             ctcagactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                <  3:671766/141‑1 (MQ=255)
                             ctcagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAAATACAGATac                                                                                                                >  2:50237/1‑140 (MQ=255)
                             ctcagactcagacagcgactcagactcagaTAGAGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAg                                                                                                                >  4:662021/1‑141 (MQ=255)
                               cagactcagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGACTCAGATAgcg                                                                                                              >  4:483708/1‑139 (MQ=255)
                                agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                                             <  2:79504/141‑1 (MQ=255)
                                agactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGa                                                                                                             <  4:570216/141‑1 (MQ=255)
                                 gactcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAt                                                                                                            >  1:1608020/1‑141 (MQ=255)
                                    tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                                                                                         >  1:200686/1‑141 (MQ=255)
                                    tcagacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCa                                                                                                         <  1:1425212/141‑1 (MQ=255)
                                     cagacagcgactcagactcagaTAGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAg                                                                                                        >  2:746827/1‑141 (MQ=255)
                                      agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                                       >  2:228136/1‑141 (MQ=255)
                                      agacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                                       >  1:1008572/1‑141 (MQ=255)
                                      agacagcgactcagactcagaTAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGa                                                                                                       <  2:1004730/141‑1 (MQ=255)
                                       gacagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGAc                                                                                                      >  1:1400498/1‑141 (MQ=255)
                                         cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                    <  3:489764/141‑1 (MQ=255)
                                         cagcgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTc                                                                                                    <  1:262482/141‑1 (MQ=255)
                                          agtgactcagattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCa                                                                                                   <  2:1608011/138‑1 (MQ=255)
                                            cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATATCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                 <  1:1213417/141‑1 (MQ=255)
                                            cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                 >  1:1000683/1‑141 (MQ=255)
                                            cgactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGa                                                                                                 <  3:456746/141‑1 (MQ=255)
                                             gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGAt                                                                                                >  1:152813/1‑141 (MQ=255)
                                             gactcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACCCACATAGCGTATCAGAGACCGATAGCGATTCGGATACACATAGCGCCTCAGATCCAGATAGTGATTCAGACTCAGAt                                                                                                <  2:1226049/141‑1 (MQ=17)
                                              actcagactcagaTAGTGACTCAGAATCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATa                                                                                               <  2:484879/141‑1 (MQ=255)
                                               ctcagactcagaTGGTGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                                                                              <  2:644100/141‑1 (MQ=255)
                                               ctcagactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAg                                                                                              >  2:1414064/1‑141 (MQ=255)
                                                   gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGGCTCAGATAGCGAc                                                                                          >  3:102199/1‑141 (MQ=255)
                                                   gactcagaTAGTGACTCAGATTCAGATAGCGACTCAGAGGCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGAc                                                                                          >  1:44493/1‑141 (MQ=255)
                                                     ctcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTc                                                                                        <  2:82373/141‑1 (MQ=255)
                                                      tcagaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                                                                       >  1:617808/1‑141 (MQ=255)
                                                         gaTAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAt                                                                                    >  3:337317/1‑141 (MQ=255)
                                                            aGTGACACAGATTCAGATACCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCa                                                                                 <  2:519944/141‑1 (MQ=255)
                                                              tGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGa                                                                               <  2:1418071/141‑1 (MQ=255)
                                                               gACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGAc                                                                              <  1:388154/141‑1 (MQ=255)
                                                                 cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGAtt                                                                                                           >  1:1064836/1‑110 (MQ=255)
                                                                 cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAg                                                                            >  2:510028/1‑141 (MQ=255)
                                                                    agattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGa                                                                         <  1:344436/141‑1 (MQ=255)
                                                                      attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACt                                                                       <  1:126284/141‑1 (MQ=255)
                                                                       ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc                                                                      >  1:1459692/1‑141 (MQ=255)
                                                                       ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGAATCAGATAGTGATGCAGACTCAGATAGCGAATCAGATTCAGACAGCGACTc                                                                      >  1:897818/1‑141 (MQ=255)
                                                                       taccaatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTc                                                                      <  2:1521064/136‑1 (MQ=255)
                                                                        tcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCa                                                                     <  1:267203/141‑1 (MQ=255)
                                                                         cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGGTTCGGACTCAGATAGCGAACCAGATTCAGATAGTGAATCAGACGCAGATAGCGACACAGACTCAGACAACCAATCAg                                                                    >  2:345491/1‑141 (MQ=14)
                                                                         cagacAGCGACTCAGAATCAGATAGCGATTCAGAATCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAg                                                                    >  3:192396/1‑141 (MQ=14)
                                                                          agatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGa                                                                   >  4:587980/1‑141 (MQ=255)
                                                                          agatAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGa                                                                   <  4:123616/141‑1 (MQ=255)
                                                                           gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTTAGATTCAGATAGCGATTCGGACTCAGATAGCGACtc                                                                                                                            <  3:243137/83‑1 (MQ=255)
                                                                           gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCa                                                                                       <  1:504633/120‑1 (MQ=255)
                                                                                  aCTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAGTCAGACAGCGACTCAGATTCGGATa                                                           >  2:836315/1‑141 (MQ=255)
                                                                                    tCAGATACGGCAAGCGATTCAGACTCAGATGGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGc                                                         <  4:403196/141‑1 (MQ=17)
                                                                                       gagtccgACAGCGATTCATACTCAGATAGCGTCTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                                                      <  4:154644/135‑1 (MQ=255)
                                                                                       gATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                                                      <  1:228136/141‑1 (MQ=255)
                                                                                       gATTCAGACACGGATTCCGATTCAGATACCGACTCAGATTCAGATACCGATTCGGATTCAGATACAGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGAc                                                      <  2:108481/141‑1 (MQ=14)
                                                                                           cGGACAGCGATTCAGACTCAGATAGCGACTCCGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAg                                                  >  3:26649/1‑141 (MQ=255)
                                                                                           aggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAg                                                  >  1:1574046/2‑141 (MQ=255)
                                                                                            ggACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGa                                                 >  1:841936/1‑141 (MQ=255)
                                                                                             gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGCGACTCAGAt                                                >  2:226031/1‑141 (MQ=255)
                                                                                                ggCGATTGAGACTCAGATAGCGACTCAGATTCAGATAGCGAGTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCa                                             <  1:50863/140‑1 (MQ=255)
                                                                                                 gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATACTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACACAGATGCGGATAGCGACGCAGATTCAg                                            >  2:1192241/1‑141 (MQ=17)
                                                                                                  cGAGTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCCGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGa                                           <  2:1609249/141‑1 (MQ=255)
                                                                                                      tCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAgg                                       >  2:124755/1‑140 (MQ=255)
                                                                                                        aGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGa                                     <  4:283399/141‑1 (MQ=255)
                                                                                                         gACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGAc                                    >  1:656575/1‑141 (MQ=255)
                                                                                                         gACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGAGTCAGACAGCGACTCAGACTCGGATAGCGACTCAGACTCAGACAGCGAc                                    >  4:234301/1‑141 (MQ=255)
                                                                                                           cTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATCGCGAATCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTc                                  >  3:63891/1‑141 (MQ=255)
                                                                                                            tCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCa                                 >  2:1360264/1‑141 (MQ=255)
                                                                                                             cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                <  3:407666/141‑1 (MQ=255)
                                                                                                             cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                <  1:697539/141‑1 (MQ=255)
                                                                                                             cAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAg                                >  2:397667/1‑141 (MQ=255)
                                                                                                                   gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCgg                          <  1:1273576/141‑1 (MQ=255)
                                                                                                                   gCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCgg                          >  1:1018597/1‑141 (MQ=255)
                                                                                                                   gCGACTCAGATTCAGATAGCGATTCGGACTAAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTGAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTAAGACAGCGACTCAGACGCgg                          >  1:765115/1‑141 (MQ=255)
                                                                                                                     gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAt                        >  2:298706/1‑141 (MQ=255)
                                                                                                                     gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGAt                        <  2:1272094/141‑1 (MQ=255)
                                                                                                                       cTCAGATTGAGATAGCGACTCGGACCCAGATAGCGACTCAGATTCAGATAGTGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAg                      <  2:10189/141‑1 (MQ=17)
                                                                                                                       cTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAg                      <  3:125121/141‑1 (MQ=255)
                                                                                                                        tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTTGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGt                     <  1:177988/141‑1 (MQ=255)
                                                                                                                         cagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTg                    >  1:717388/1‑141 (MQ=255)
                                                                                                                                     gCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAg        <  3:213397/141‑1 (MQ=255)
                                                                                                                                         ttCGGACTCAGATTGTGACTCAGATTCAGATAGTGAGACAGACACAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAg    <  2:888131/141‑1 (MQ=18)
                                                                                                                                         ttCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGAATCAGATAGTGAGTCAGATTCAGACAGTGa                   >  3:179667/1‑126 (MQ=14)
                                                                                                                                          tCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGACAGc   >  4:378714/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                           
TCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGTGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGATTCGGATAGCGACTCAGATTCAGACAGCGACTCAGACTCGGATAGTGATTCAGACTCAGATAGCG  >  USA300TCH1516_ALE/618496‑618775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: