Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,479 T→C 7.1% D1014D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,4790TC 7.1% 469.5 / 4.0 177D1014D (GAT→GACsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (83/78);  new base C (7/8);  total (90/87)
Fisher's exact test for biased strand distribution p-value = 7.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 179 total aligned reads displayed.

GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAC  >  USA300TCH1516_ALE/622339‑622617
                                                                                                                                            |                                                                                                                                          
gattcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGAt                                                                                                                                            >  1:139280/1‑141 (MQ=255)
  ttcagaCAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTc                                                                                                                                          <  3:547475/141‑1 (MQ=255)
          gCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAg                                                                                                                                  >  1:1346086/1‑141 (MQ=255)
             acacagactcagatAGCGACTGAGATTCGGACTGCGATTCAGACTCAGATAGCGACAGAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCGGAtg                                                                                                                               <  2:702/138‑2 (MQ=14)
              cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCGGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                              >  1:54715/1‑141 (MQ=255)
              cTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAg                                                                                                                              <  1:1004952/141‑1 (MQ=255)
               tcagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                             <  4:284659/141‑1 (MQ=255)
                cagattcagatAGCGATTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCGGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                            >  2:429815/1‑141 (MQ=255)
                cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                            <  4:255132/141‑1 (MQ=255)
                cagattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCg                                                                                                                            <  2:779112/141‑1 (MQ=255)
                cagattcagatAGCGACTCAGATTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCCGACAGCGACTCAGATCCAGATAGCGATTCGGACACAGACCGGGATTCAGACTCAGGGAGCg                                                                                                                            >  2:969174/1‑141 (MQ=14)
                  gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                                                          <  1:181890/141‑1 (MQ=255)
                  gattcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGAGTCAGATAGCgac                                                                                                                          >  2:509613/1‑141 (MQ=255)
                   attcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgact                                                                                                                         <  2:426394/141‑1 (MQ=255)
                    ttcagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATCCAGACTCAGAAAACgacac                                                                                                                        >  4:337372/1‑139 (MQ=255)
                     tcagatAGCAACTCAGATTCGGACAGCGATTCAGACTCCGATAGCGACTCGGCTTCAGATAGCGGTTCAGCGTCAAACAGCGACTCAGATTCAGGTAGCGATTCGTGCTCAGTCATCGATTCAGCCCAAGAGAGCgactcg                                                                                                                       >  2:181890/1‑140 (MQ=17)
                      cagatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcag                                                                                                                      <  1:483311/141‑1 (MQ=255)
                       agatgtcGACTCAGATTCGGACAGTGAGTCCGAATCAGAGAGCGACTAAGATACAGATAGCGAGTCAGATTCAGACAGCGACTCAGATTCGGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                                                                                     <  2:1101814/135‑1 (MQ=14)
                        gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                                    >  1:1111693/1‑141 (MQ=255)
                        gatAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagac                                                                                                                    <  2:70490/141‑1 (MQ=255)
                          tAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                                                  >  1:584465/1‑141 (MQ=255)
                            gCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                                                >  3:285513/1‑141 (MQ=255)
                            ccGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCACATAGCGATTCAGACTCATACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                                                                <  4:609592/140‑1 (MQ=255)
                             cGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGActcagactcaga                                                                                                               <  1:1410107/141‑1 (MQ=255)
                              gACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGCCATCGATTCAGACTCAGATAGCGACtcagactcagac                                                                                                              <  1:465665/141‑1 (MQ=255)
                                 tCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGc                                                                                                           >  3:379926/1‑141 (MQ=255)
                                          gACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagat                                                                                                  >  1:1156689/1‑141 (MQ=255)
                                            cAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattc                                                                                                <  1:1117915/141‑1 (MQ=255)
                                              gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcag                                                                                              >  1:833255/1‑141 (MQ=255)
                                               cGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcaga                                                                                             >  1:786428/1‑141 (MQ=255)
                                                gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACtcagattcagat                                                                                            <  3:565236/141‑1 (MQ=255)
                                                    cAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGTGATTCGGACTCCGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCg                                                                                        >  1:38077/1‑141 (MQ=255)
                                                      gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                      >  1:1440616/1‑141 (MQ=255)
                                                        cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                                    <  3:324649/141‑1 (MQ=255)
                                                           aGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                 <  2:160218/141‑1 (MQ=255)
                                                               aGCGACTCACACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCa                                                                             <  4:506770/141‑1 (MQ=11)
                                                                gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAg                                                                            >  2:1115504/1‑141 (MQ=255)
                                                                  gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                          >  1:205291/1‑141 (MQ=255)
                                                                    cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAg                                                                        >  2:1328877/1‑141 (MQ=255)
                                                                    cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGATAGCGAGTCGGACTCagagag                                                                        >  2:1157655/1‑141 (MQ=255)
                                                                     tcagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                       <  4:317272/141‑1 (MQ=255)
                                                                      cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                      >  1:103034/1‑141 (MQ=255)
                                                                       agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                     <  2:1357314/141‑1 (MQ=255)
                                                                       agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACACAGACAGCGa                                                                     >  2:1399175/1‑141 (MQ=255)
                                                                       agattcagacAGCGATTAAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                     <  2:903661/141‑1 (MQ=255)
                                                                        gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCCGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGACAGCGAc                                                                    >  2:1084110/1‑141 (MQ=255)
                                                                        gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                      <  2:821417/139‑1 (MQ=255)
                                                                        gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                    <  1:1082859/141‑1 (MQ=255)
                                                                          ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                  >  1:1204396/1‑141 (MQ=255)
                                                                          ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                  <  2:1566639/141‑1 (MQ=255)
                                                                           tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                 >  1:870425/1‑141 (MQ=255)
                                                                            cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                <  1:705186/141‑1 (MQ=255)
                                                                                 aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGACACAGACAGCGACTCAGATCCAGAGAGCGACTCGGACTCAGACAGCGACTCAGacaca                                                           >  2:297258/1‑137 (MQ=14)
                                                                                  gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAg                                                          >  4:169527/1‑141 (MQ=255)
                                                                                   cGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGa                                                         <  2:227955/141‑1 (MQ=255)
                                                                                    gATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                                        <  1:398393/141‑1 (MQ=255)
                                                                                    gATGCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                                        <  4:588681/141‑1 (MQ=255)
                                                                                       tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGc                                                     >  1:945521/1‑141 (MQ=255)
                                                                                        cagactcagacAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATACAGACTCAGAACCCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGAGATTCAGACACAGGCAGCGACTCAGATTCCGACAGCg                                                    >  3:837/1‑141 (MQ=11)
                                                                                          gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                  <  2:464268/141‑1 (MQ=255)
                                                                                          gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGAGACTCGGACGCAGACGGCGACTCAGCGTCAGAGAGCGaa                                                  >  2:128767/1‑140 (MQ=11)
                                                                                           gcgcagacAGCGACTCAGACTCGGACAGCCACTCTGACTCAGACAGAGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                 <  2:766797/138‑1 (MQ=11)
                                                                                           actcagatAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATACCCATTCGGACTCAGATCGACACTCAGATTCAAACACCGact                                                 >  4:61235/1‑139 (MQ=11)
                                                                                            ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                                                        <  2:952136/69‑1 (MQ=255)
                                                                                            ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTc                                                <  3:51765/141‑1 (MQ=255)
                                                                                              ctgacTGCGACTCAGATTCAGACAGCGATTCTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                              <  2:730553/139‑1 (MQ=255)
                                                                                              cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                              >  1:934652/1‑141 (MQ=255)
                                                                                              cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                              >  1:934688/1‑141 (MQ=255)
                                                                                                  cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                          >  1:791593/1‑141 (MQ=255)
                                                                                                  cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                          >  3:565969/1‑141 (MQ=255)
                                                                                                    gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                        >  2:266033/1‑141 (MQ=255)
                                                                                                     cGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGa                                       <  2:1006821/141‑1 (MQ=255)
                                                                                                      gACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                      >  2:796447/1‑141 (MQ=255)
                                                                                                      cactcagactcagACAGCGCGTCGGACTCAGACTCTGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                      <  2:1185904/140‑1 (MQ=14)
                                                                                                        cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                    >  1:1321208/1‑141 (MQ=255)
                                                                                                           agcttcagatAGCGGTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                                 <  4:68100/138‑1 (MQ=255)
                                                                                                            gattcagatACCGACGCGGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                <  4:388076/141‑1 (MQ=14)
                                                                                                              ttcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                              <  2:791593/141‑1 (MQ=255)
                                                                                                                 agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga                           <  1:702154/141‑1 (MQ=255)
                                                                                                                  gatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagac                          >  1:637297/1‑141 (MQ=255)
                                                                                                                    tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGCCTCAGATTCAGATAGCGCTTCGGAGTCAGTTAGCGGCTCAGATTCAGACAGCGGTTCAGACTCAGATAGCgactcagcctc                        >  3:203139/1‑141 (MQ=17)
                                                                                                                    tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcagactc                        >  1:1648897/1‑141 (MQ=255)
                                                                                                                    tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        >  1:581669/1‑141 (MQ=255)
                                                                                                                    cagtgattcagactcagatagcgattcagactcagatagcGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                        >  2:1285610/5‑141 (MQ=255)
                                                                                                                      gtgaggcGGACTCACACACCGCCTCAGACGCAGAGAGCGACTCAGACTCAGACAGCGACTAAGAGTCAGACAGCGACTCAGAATCAGATAGAGATACAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                      <  4:630924/135‑1 (MQ=11)
                                                                                                                      gCGATTCGGACGCAGACAGCGAGGCAGATGAAGATAGCGCATCTGACTAAGACAGCGCCTCAGACTCAGCCAGCGCTTCGGACTCAGATAGTGACTCTGATTCAGACAGCGATTCAGACTCAGATAGCGactcatactcag                      <  4:417886/141‑1 (MQ=12)
                                                                                                                        gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                    <  1:515326/141‑1 (MQ=255)
                                                                                                                         aTTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGActcagactcagata                   <  4:561087/141‑3 (MQ=255)
                                                                                                                          ttCGGACTCAGACAGCGATTCAGACGCAGATAGCGATGCATGGTCAGGCAACGGCTCAGATTCAGATAGCTATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAg                  <  1:1073547/141‑1 (MQ=14)
                                                                                                                            cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  2:356317/1‑141 (MQ=255)
                                                                                                                            cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                >  3:446370/1‑141 (MQ=255)
                                                                                                                             ggACTCAGCAAGCGATGCAGACTCAGATAGCGAATCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               <  2:887288/141‑1 (MQ=17)
                                                                                                                             ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               <  1:1495521/141‑1 (MQ=255)
                                                                                                                             ggACTCAGACAGCGATTCAGACGCAGATAGCGCCTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga               <  4:469497/141‑1 (MQ=255)
                                                                                                                                    gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat        >  2:1557137/1‑141 (MQ=255)
                                                                                                                                    gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAGATAGCGACTCCGACTCAGACAGTgagtcagat        >  2:279239/1‑141 (MQ=255)
                                                                                                                                     aCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagatt       >  4:648133/1‑141 (MQ=255)
                                                                                                                                        gCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagactgcg    >  2:399716/1‑136 (MQ=255)
                                                                                                                                         cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga   <  1:889790/141‑1 (MQ=255)
                                                                                                                                          gATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCCGAc  >  2:810374/1‑141 (MQ=255)
                                                                                                                                            |                                                                                                                                          
GATTCAGACAGCGACTCAGATTCAGATAGCGACTCAGATTCGGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGAC  >  USA300TCH1516_ALE/622339‑622617

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: