Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 622,533 T→C 7.9% D1032D (GAT→GAC sdrE → Serine‑aspartate repeat‑containing protein E

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE622,5330TC 7.9% 446.3 / 6.1 178D1032D (GAT→GACsdrESerine‑aspartate repeat‑containing protein E
Reads supporting (aligned to +/- strand):  ref base T (85/74);  new base C (13/5);  total (99/79)
Fisher's exact test for biased strand distribution p-value = 1.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

GACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAG  >  USA300TCH1516_ALE/622393‑622673
                                                                                                                                            |                                                                                                                                            
gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGTTTCACATGCAGATAGCGATTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCACGCAGCGACTCAGATTCAGATAGCGAt                                                                                                                                              <  3:235384/141‑1 (MQ=14)
gACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGAt                                                                                                                                              >  1:1440616/1‑141 (MQ=255)
  cTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTc                                                                                                                                            <  3:324649/141‑1 (MQ=255)
    cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCgg                                                                                                                                          <  2:730086/141‑1 (MQ=255)
    cAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGAGAGTCAGACTGAGAGAGCGACTCAGATTCAGATAGCGATTCgg                                                                                                                                          >  2:398393/1‑141 (MQ=255)
     agacagcgactcagactcagacagcgactcagactcAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGa                                                                                                                                         <  4:637222/141‑1 (MQ=255)
        tAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTc                                                                                                                                      >  1:552299/1‑141 (MQ=255)
         aGCGCCTGAGATTAAGATAGCGAATCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACTCAGAGAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACtaa                                                                                                                                     <  4:502623/141‑3 (MQ=14)
          gCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAg                                                                                                                                    >  2:1115504/1‑141 (MQ=255)
            gACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGAt                                                                                                                                  >  1:205291/1‑141 (MQ=255)
              cTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAg                                                                                                                                <  3:467029/141‑1 (MQ=255)
               tcagatgcagatAGCGATTCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGc                                                                                                                               <  4:317273/141‑1 (MQ=255)
                cagattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCg                                                                                                                              >  1:103034/1‑141 (MQ=255)
                 agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                             <  2:1357314/141‑1 (MQ=255)
                 agattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACACAGACAGCGa                                                                                                                             >  2:1399175/1‑141 (MQ=255)
                 agattcagatAGCGATTCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGa                                                                                                                             <  2:1335241/141‑1 (MQ=255)
                  gattcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGAc                                                                                                                            <  4:513465/141‑1 (MQ=255)
                    ttcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTc                                                                                                                          >  1:1204396/1‑141 (MQ=255)
                     tcagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCa                                                                                                                         >  1:870425/1‑141 (MQ=255)
                      cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAg                                                                                                                        <  1:705145/141‑1 (MQ=255)
                      cagatAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAg                                                                                                                        >  2:887717/1‑141 (MQ=255)
                      cagatAGCGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCGGACTCAGACAGCGACTCAGATTCAGATAGCGACTGGGACTCAGATAGCGACTCAg                                                                                                                        >  2:116766/1‑141 (MQ=14)
                           aGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGAAAGCGACTCAGACACAGACAGCGACTCAGATCCAGAGAGCGACTCGGACTCAGACAGCGACTCAGacaca                                                                                                                   >  2:297258/1‑137 (MQ=14)
                            gCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAg                                                                                                                  >  4:169527/1‑141 (MQ=255)
                              gATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGAc                                                                                                                <  1:398393/141‑1 (MQ=255)
                                 tcagactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGc                                                                                                             >  1:945521/1‑141 (MQ=255)
                                  cagactcagacAGCGACTCAGACTCAGATAGCGATTCGGACTCAGACAGCGATACAGACTCAGAACCCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGAGATTCAGACACAGGCAGCGACTCAGATTCCGACAGCg                                                                                                            >  3:837/1‑141 (MQ=11)
                                    gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAt                                                                                                          <  2:464268/141‑1 (MQ=255)
                                     gcgcagacAGCGACTCAGACTCGGACAGCCACTCTGACTCAGACAGAGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                         <  2:766797/138‑1 (MQ=11)
                                     attcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGAtt                                                                                                         >  2:804295/3‑141 (MQ=255)
                                     actcagatAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATACCCATTCGGACTCAGATCGACACTCAGATTCAAACACCGact                                                                                                         >  4:61235/1‑139 (MQ=11)
                                        cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAg                                                                                                      >  1:934652/1‑141 (MQ=255)
                                          gacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGAc                                                                                                    <  1:1079048/141‑1 (MQ=255)
                                            cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                  >  1:791593/1‑141 (MQ=255)
                                            cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                  >  1:134269/1‑141 (MQ=255)
                                            cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                  >  3:565969/1‑141 (MQ=255)
                                            cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTc                                                                                                  <  2:730677/141‑1 (MQ=255)
                                              gCGACTCAGATTCAGATAGCGATTCGGACTCAGAGAGCGCTTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCTAATACAGACAGCGACGCAGACTCAg                                                                                                >  2:575989/1‑141 (MQ=14)
                                              gCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGACTCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGactcagactcag                                                                                                >  2:266033/1‑141 (MQ=255)
                                                cactcagactcagACAGCGCGTCGGACTCAGACTCTGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGAt                                                                                              <  2:1185904/140‑1 (MQ=14)
                                                  cTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAg                                                                                            >  1:1321208/1‑141 (MQ=255)
                                                     agcttcagatAGCGGTTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCga                                                                                         <  4:68100/138‑1 (MQ=255)
                                                      gattcagatACCGACGCGGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgac                                                                                        <  4:388076/141‑1 (MQ=14)
                                                        ttcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                      <  2:791593/141‑1 (MQ=255)
                                                        ttcagatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                      >  4:308087/1‑141 (MQ=255)
                                                           agatAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcaga                                                                                   <  1:1119414/141‑1 (MQ=255)
                                                           agacAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCCGACACAGATAGCGACTCAGATTCAGACAGCGACACAGACCCAGATAGCgactcaga                                                                                   >  2:257799/1‑141 (MQ=11)
                                                              tAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAGCgactcagactc                                                                                >  1:1648897/1‑141 (MQ=255)
                                                              cagtgattcagactcagatagcgattcagactcagatagcGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                >  2:1285610/5‑141 (MQ=255)
                                                              caGCGATTGTGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGAGAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactcagactc                                                                                <  4:575941/140‑1 (MQ=255)
                                                                gtgaggcGGACTCACACACCGCCTCAGACGCAGAGAGCGACTCAGACTCAGACAGCGACTAAGAGTCAGACAGCGACTCAGAATCAGATAGAGATACAGATTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                              <  4:630924/135‑1 (MQ=11)
                                                                gCGATTCGGACGCAGACAGCGAGGCAGATGAAGATAGCGCATCTGACTAAGACAGCGCCTCAGACTCAGCCAGCGCTTCGGACTCAGATAGTGACTCTGATTCAGACAGCGATTCAGACTCAGATAGCGactcatactcag                                                                              <  4:417886/141‑1 (MQ=12)
                                                                gCGATGCGGACGCAGAAAGCGCCTAAGACTCAGATATCGACGCAGACTCAGACAGCGACTCAGATTCAGAAAGCGACTCGGACTCAGACAGCGACCCAGAGTCAGACAGCGATTCAGACTCAGATAGCGactcagactcag                                                                              <  4:417747/141‑1 (MQ=14)
                                                                  gATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACtcagactcagac                                                                            <  1:515326/141‑1 (MQ=255)
                                                                      cGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTg                                                                        >  3:446370/1‑141 (MQ=255)
                                                                       ggACTCAGCAAGCGATGCAGACTCAGATAGCGAATCAGACTCAGACAGCGACGCAGATTCAGATAGCGATTCTGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                       <  2:887288/141‑1 (MQ=17)
                                                                       ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                       <  1:1495521/141‑1 (MQ=255)
                                                                       ggACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTga                                                                       <  1:1600242/141‑1 (MQ=255)
                                                                         aCTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgatt                                                                     >  2:1032420/1‑141 (MQ=255)
                                                                          cTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattc                                                                    >  3:122724/1‑141 (MQ=255)
                                                                             aGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcaga                                                                 >  2:811447/1‑141 (MQ=255)
                                                                              gACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGTGATTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACAGCGACTCAGAGTCAGACGGCgaatcagaa                                                                >  2:224717/1‑140 (MQ=11)
                                                                              gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagat                                                                >  2:1557137/1‑141 (MQ=255)
                                                                              gACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACCCAGATAGCGACTCCGACTCAGACAGTgagtcagat                                                                >  2:279239/1‑141 (MQ=255)
                                                                               aCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagatt                                                               >  4:648133/1‑141 (MQ=255)
                                                                                cAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGACTCGGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGACTCAGACAGTgattcagattc                                                              <  2:775002/141‑1 (MQ=255)
                                                                                   cGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGAttcagattcaga                                                           <  1:889790/141‑1 (MQ=255)
                                                                                        cAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACACAGATACAGACAAAGACTCAGACTCAGATAGCGAATAAGATGAAGACAGTGAATCAGATTCAGACAACg                                                      >  4:305583/1‑141 (MQ=14)
                                                                                            cTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactc                                                  >  1:639045/1‑141 (MQ=255)
                                                                                                   aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactca                                           >  2:867328/1‑141 (MQ=255)
                                                                                                   aGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCgactcagactca                                           <  2:139280/141‑1 (MQ=255)
                                                                                                      gactcagactcaGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGACTCAGATTCAGACAGCGACTCAGACACAGAt                                        >  3:628515/1‑141 (MQ=255)
                                                                                                        ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATaa                                      >  4:44371/1‑140 (MQ=255)
                                                                                                        ctcagactcagaCAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAg                                      >  4:561670/1‑141 (MQ=255)
                                                                                                         tcagactcagacAGCGACTCAGATTCAGAAAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGc                                     >  2:91880/1‑141 (MQ=255)
                                                                                                           agactcagacAGCGACTCAGATGCAGATAGCGATTCGGACTCAGATAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGa                                   <  2:1156196/141‑1 (MQ=255)
                                                                                                            gactcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGAc                                  >  3:562626/1‑141 (MQ=255)
                                                                                                              ctcagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACCCAGACTCAGATAACGAATc                                >  2:1323989/1‑141 (MQ=255)
                                                                                                                cagacAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAg                              <  1:246575/141‑1 (MQ=255)
                                                                                                                    cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCga                                                     >  2:1621433/1‑114 (MQ=255)
                                                                                                                    cAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                          >  1:376613/1‑141 (MQ=255)
                                                                                                                    cAGCGACGCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTc                          <  2:1592115/141‑1 (MQ=14)
                                                                                                                     aGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCg                         <  3:191621/141‑1 (MQ=255)
                                                                                                                       cGACTCAGAGTCCGACAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGa                       <  4:541061/141‑1 (MQ=255)
                                                                                                                        gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                      >  1:1069673/1‑141 (MQ=255)
                                                                                                                        gACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGAc                      >  2:650602/1‑141 (MQ=255)
                                                                                                                           tcagattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGc                   <  2:1204396/141‑1 (MQ=255)
                                                                                                                              gattcatatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGAc                <  1:62728/141‑1 (MQ=255)
                                                                                                                              gattcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGAc                >  2:399807/1‑141 (MQ=255)
                                                                                                                               attcagatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACt               <  1:519885/141‑1 (MQ=255)
                                                                                                                                   agatAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCTGACAGCGACTCAGa           <  3:308087/141‑1 (MQ=255)
                                                                                                                                      tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActc        >  1:1166722/1‑141 (MQ=255)
                                                                                                                                      tAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGACTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGAGacacacacaa        >  2:486915/1‑139 (MQ=17)
                                                                                                                                       aGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGCCTCAGACTCAGACAGTGATTCAGCATCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActct       <  1:746800/141‑1 (MQ=255)
                                                                                                                                       aGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGActct       <  1:785707/141‑1 (MQ=255)
                                                                                                                                        gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCgactc                                                                                      >  1:278735/1‑61 (MQ=255)
                                                                                                                                        gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg      >  1:841025/1‑141 (MQ=255)
                                                                                                                                        gCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTg      >  2:1542271/1‑141 (MQ=255)
                                                                                                                                            ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTTATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg  >  1:30226/1‑141 (MQ=255)
                                                                                                                                            ttCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAg  <  1:96345/141‑1 (MQ=255)
                                                                                                                                            |                                                                                                                                            
GACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGCGACTCAGATTCAGATAGCGATTCGGACTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGACAGTGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGACAGCGACTCAGACTCTGATAG  >  USA300TCH1516_ALE/622393‑622673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: