BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,584,513	A→T	5.5%	S400S (TCT→TCA)	USA300HOU_RS07765 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,584,513	0	A	T	5.5%	597.6 / 5.5	187	S400S (TCT→TCA)	USA300HOU_RS07765	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (77/95); new base T (11/2); total (89/98)
Fisher's exact test for biased strand distribution p-value = 7.66e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

AAGTAATTTTTTCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGTCTGCCTTC  >  USA300TCH1516_ALE/1584377‑1584653

 aGTAATTTTTTCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAata                                                                                                                                         <  1:796907/141‑1 (MQ=255)

 aGTAATTTTTTCTACTACATAATGCTCATACATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTAGTTAGAata                                                                                                                                         >  2:1220846/1‑141 (MQ=255)

   tAATTTTTTCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATAtt                                                                                                                                       <  4:503337/141‑1 (MQ=255)

    aaTTTTTTCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTc                                                                                                                                      <  1:1154823/141‑1 (MQ=255)

        ttttCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACt                                                                                                                                  <  1:10205/141‑1 (MQ=255)

        ttttCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACt                                                                                                                                  <  4:621416/141‑1 (MQ=255)

         tttCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACtt                                                                                                                                 <  2:1481382/141‑1 (MQ=255)

           tCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTtg                                                                                                                               <  1:210554/141‑1 (MQ=255)

              actacATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGc                                                                                                                            <  1:472959/141‑1 (MQ=255)

                tacATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCtt                                                                                                                          <  1:885040/141‑1 (MQ=255)

                tacATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCtt                                                                                                                          <  2:1349857/141‑1 (MQ=255)

                 acATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTa                                                                                                                         <  2:1626656/141‑1 (MQ=255)

                 acATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTa                                                                                                                         <  2:1470176/141‑1 (MQ=255)

                 acATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTa                                                                                                                         <  3:11641/141‑1 (MQ=255)

                  cATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAg                                                                                                                        >  1:1264579/1‑141 (MQ=255)

                     aaTGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGaa                                                                                                                     <  1:889793/141‑1 (MQ=255)

                     aaTGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGaa                                                                                                                     <  4:597448/141‑1 (MQ=255)

                       tGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATc                                                                                                                   >  1:1221428/1‑141 (MQ=255)

                        gCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATcc                                                                                                                  >  1:221505/1‑141 (MQ=255)

                         cTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCa                                                                                                                 <  3:328321/141‑1 (MQ=255)

                         cTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCa                                                                                                                 <  1:273811/141‑1 (MQ=255)

                          tcattcatCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTGTCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACtt                                                                                                                                 >  1:155283/1‑124 (MQ=255)

                              tcatCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAAt                                                                                                            <  2:945834/141‑1 (MQ=255)

                               catCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATg                                                                                                           <  2:181832/141‑1 (MQ=255)

                                atCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTTTTTATATTTTTAAACTTTGTGCGTATGAGTTCATGAATGc                                                                                                          >  2:698997/1‑141 (MQ=255)

                                atCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGc                                                                                                          <  1:350227/141‑1 (MQ=255)

                                atCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGc                                                                                                          >  4:104444/1‑141 (MQ=255)

                                  cTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGa                                                                                                        <  1:1354564/141‑1 (MQ=255)

                                     aaTTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATaa                                                                                                     >  2:1022963/1‑141 (MQ=255)

                                         aTCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAAc                                                                                                                                   <  3:430306/107‑1 (MQ=255)

                                         aTCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACt                                                                                                 >  2:1141705/1‑141 (MQ=255)

                                          tCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTg                                                                                                >  2:525096/1‑141 (MQ=255)

                                          tCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTg                                                                                                <  3:456619/141‑1 (MQ=255)

                                           ccAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGc                                                                                               >  3:638261/1‑141 (MQ=255)

                                            cAATTTAAATCATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCa                                                                                              <  4:175658/141‑1 (MQ=255)

                                            cAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCa                                                                                              >  2:1654311/1‑141 (MQ=255)

                                               tttAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACt                                                                                           <  2:1350119/141‑1 (MQ=255)

                                                ttAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACtt                                                                                          >  3:230314/1‑141 (MQ=255)

                                                   aaaCATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATAACAAAAAGTGCTTAGGAATCCATGAATGCGATAAAACTGCAANTTTAc                                                                                       >  1:14979/1‑141 (MQ=255)

                                                    aaCATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTAcc                                                                                      >  1:84252/1‑141 (MQ=255)

                                                         cTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATcc                                                                                                                  >  1:1423374/1‑108 (MQ=255)

                                                          ttGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATc                                                                                <  2:1210994/141‑1 (MQ=255)

                                                           tGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCt                                                                               >  3:130666/1‑141 (MQ=255)

                                                            ggTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTa                                                                              >  1:1253431/1‑141 (MQ=255)

                                                            ggTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTa                                                                              >  3:504280/1‑141 (MQ=255)

                                                            ggTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTCCAACTTTACCATTATCTa                                                                              >  4:313023/1‑141 (MQ=255)

                                                              tAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAAc                                                                            <  2:243861/141‑1 (MQ=255)

                                                              tAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAAc                                                                            <  2:243630/141‑1 (MQ=255)

                                                              tAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAAc                                                                            <  2:1518705/141‑1 (MQ=255)

                                                               aatCAATATACGGTTTATCTTGAATAGTTAGTAAGCCATCTTCTTCCCATTCTCTATATGGTATTTTTTCATTTGAGTACTCTACCTTATGCTTAGGAATCCATGAATGTGTTAAGACAGCAACTTTACCATTATCTAACg                                                                           <  2:356825/139‑1 (MQ=255)

                                                                 tCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCa                                                                         >  1:1295758/1‑141 (MQ=255)

                                                                   aaTATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCaaa                                                                       >  1:245499/1‑141 (MQ=255)

                                                                   aaTATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCaaa                                                                       >  1:612883/1‑141 (MQ=255)

                                                                       taAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGCATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTa                                                                   >  1:952993/1‑141 (MQ=255)

                                                                        aaGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAg                                                                  <  1:1633878/141‑1 (MQ=255)

                                                                          ggCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGca                                                                <  2:1295758/141‑1 (MQ=255)

                                                                          ggCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGca                                                                >  1:405257/1‑141 (MQ=255)

                                                                           gCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGcac                                                               >  4:488325/1‑141 (MQ=255)

                                                                            cTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCTTTTTTTTTTTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGcaca                                                              >  2:63948/1‑141 (MQ=255)

                                                                            cTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGcaca                                                              >  2:1123503/1‑141 (MQ=255)

                                                                              tATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACAcg                                                            <  4:646687/141‑1 (MQ=255)

                                                                               aTCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACAcgc                                                           <  1:109331/141‑1 (MQ=255)

                                                                                  ttGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGc                                                        <  4:152847/141‑1 (MQ=255)

                                                                                    gCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAGTCCATGAATGCGATAAAGCTGCCACTTTACCATTATCTACCGCAATAGTAGCACACGCGGCTg                                                      <  2:1426262/141‑1 (MQ=255)

                                                                                    gCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTg                                                      >  2:1496273/1‑141 (MQ=255)

                                                                                    gCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTg                                                      <  2:1181297/141‑1 (MQ=255)

                                                                                    gCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTg                                                      >  1:809100/1‑141 (MQ=255)

                                                                                      aCTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTa                                                    <  2:1358835/141‑1 (MQ=255)

                                                                                           taataaGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTc                                               >  2:763257/1‑141 (MQ=255)

                                                                                            aataaGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTcc                                              <  1:1349130/141‑1 (MQ=255)

                                                                                              taaGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCctc                                            <  1:461483/141‑1 (MQ=255)

                                                                                              taaGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCctc                                            <  3:705580/141‑1 (MQ=255)

                                                                                               aaGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCctct                                           >  1:7880/1‑141 (MQ=255)

                                                                                                   cATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGttt                                       <  3:45695/141‑1 (MQ=255)

                                                                                                   cATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGttt                                       <  2:1194487/141‑1 (MQ=255)

                                                                                                    aTCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTc                                      <  3:26099/141‑1 (MQ=255)

                                                                                                       ttcttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGa                                   <  3:411011/141‑1 (MQ=255)

                                                                                                        tcttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGAt                                  <  4:159651/141‑1 (MQ=255)

                                                                                                         cttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATa                                 <  1:903923/141‑1 (MQ=255)

                                                                                                         cttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATa                                 <  2:84252/141‑1 (MQ=255)

                                                                                                         cttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATa                                 <  2:84123/141‑1 (MQ=255)

                                                                                                          ttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATc                                                                                                                   <  2:1387624/58‑1 (MQ=255)

                                                                                                          ttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATaa                                >  4:671948/1‑141 (MQ=255)

                                                                                                          ttcCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATaa                                >  1:101662/1‑141 (MQ=255)

                                                                                                             ccATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATc                             >  1:1338843/1‑141 (MQ=255)

                                                                                                                ttCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATa                          >  1:687529/1‑141 (MQ=255)

                                                                                                                  ctctATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAAc                        <  2:29104/141‑1 (MQ=255)

                                                                                                                   tctATAGGGTATTTTTTCGTTCGAATATTCAACTCTGTGCTTAGGAATCCATTAATTCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACTCGGCTGTAAAGGCCTCTGTTTCTGATAAATCATAAcc                       <  4:384332/141‑1 (MQ=255)

                                                                                                                   tctATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAAcc                       <  1:38020/141‑1 (MQ=255)

                                                                                                                    ctATAGGGTATTTTTTTTTTTGTATTTTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCa                      >  2:221003/1‑141 (MQ=255)

                                                                                                                    ctATAGGGTATTTTTTCGTTAGTATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCa                      >  2:515114/1‑141 (MQ=255)

                                                                                                                       taGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATc                   >  1:522016/1‑141 (MQ=255)

                                                                                                                        aGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCg                  <  1:1399085/141‑1 (MQ=255)

                                                                                                                               tttgtCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTGACGCAAAAGTAGCACACGCGGCTGTAAAGTCCGCTGTTTCTGATAAATCATAACCAATCGTGCATgg           <  2:1277603/141‑1 (MQ=255)

                                                                                                                                ttttCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGt          <  2:1567793/141‑1 (MQ=255)

                                                                                                                                 tttCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCAGTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATACATCATAACCAATCGTGCCTGGTc         >  2:869794/1‑141 (MQ=255)

                                                                                                                                  ttCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGTCt        <  1:1287957/141‑1 (MQ=255)

                                                                                                                                  ttCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGTCt        <  1:1339966/141‑1 (MQ=255)

                                                                                                                                       tAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGTCTGCCtt   <  1:285252/141‑1 (MQ=255)

                                                                                                                                       tAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGTCTGCCtt   <  1:1526989/141‑1 (MQ=255)

AAGTAATTTTTTCTACTACATAATGCTCATTCATCTTAATTATCCAATTTAAAACATCTTGGTAGTCAATATAAGGCTTATCTTGCACTGTTAATAAGCCATCTTCTTCCCATTCTCTATAGGGTATTTTTTCGTTAGAATATTCAACTTTGTGCTTAGGAATCCATGAATGCGATAAAACTGCAACTTTACCATTATCTAACGCAAAAGTAGCACACGCGGCTGTAAAGTCCTCTGTTTCTGATAAATCATAACCAATCGTGCATGGTCTGCCTTC  >  USA300TCH1516_ALE/1584377‑1584653

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`