BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,732,797	G→A	6.0%	E333K (GAA→AAA)	limB_2 →	Limonene 1,2‑monooxygenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,732,797	0	G	A	6.0%	527.7 / 3.1	163	E333K (GAA→AAA)	limB_2	Limonene 1,2‑monooxygenase
Reads supporting (aligned to +/- strand): ref base G (89/62); new base A (2/10); total (91/72)
Fisher's exact test for biased strand distribution p-value = 5.78e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TACTGTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTCAAATTC  >  USA300TCH1516_ALE/1732658‑1732936

tACTGTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGa                                                                                                                                              >  3:229890/1‑141 (MQ=255)

 aCTGTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGaa                                                                                                                                             <  1:680857/141‑1 (MQ=255)

 aCTGTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGaa                                                                                                                                             >  2:1428271/1‑141 (MQ=255)

    gTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGaaaaa                                                                                                                                          >  1:279993/1‑141 (MQ=255)

     ttCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGaaaaaa                                                                                                                                         >  2:236218/1‑141 (MQ=255)

         acaaAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAacac                                                                                                                                     >  4:37403/1‑141 (MQ=255)

          caaAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACt                                                                                                                                    <  1:882132/141‑1 (MQ=255)

            aaaaaTTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACttt                                                                                                                                  <  1:106100/141‑1 (MQ=255)

            aaaaaTTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACttt                                                                                                                                  >  2:927130/1‑141 (MQ=255)

              aaaTTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAANACTTta‑t                                                                                                                               >  2:1246730/1‑141 (MQ=255)

              aaaTTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTta‑t                                                                                                                               <  1:1131038/141‑1 (MQ=255)

                  tCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AAtt                                                                                                                          >  4:563070/1‑140 (MQ=255)

                  tCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AAtg                                                                                                                          >  1:842146/1‑141 (MQ=255)

                    aaGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AAtgtg                                                                                                                        >  3:196468/1‑141 (MQ=255)

                    aaGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AAtgtg                                                                                                                        <  3:709167/141‑1 (MQ=255)

                    aaGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AAtgtg                                                                                                                        >  3:672235/1‑141 (MQ=255)

                        ttttATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGataa                                                                                                                    >  2:1314810/1‑141 (MQ=255)

                          ttATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGataaat                                                                                                                  <  1:1345433/141‑1 (MQ=255)

                            aTGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGataaataa                                                                                                                <  2:1265239/141‑1 (MQ=255)

                             tGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGAtaaataaa                                                                                                               >  1:217441/1‑141 (MQ=255)

                             tGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAAAACTTTG‑T‑AATGTGAtaaataaa                                                                                                               >  1:14128/1‑141 (MQ=255)

                             tGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTACGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGAtaaataaa                                                                                                               >  4:70771/1‑141 (MQ=255)

                                 aaCTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTaa                                                                                                           >  4:538291/1‑141 (MQ=255)

                                  aCTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAg                                                                                                          <  1:1000655/141‑1 (MQ=255)

                                   cTTATGGTGAAGTCAACGAAATTATGGTAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGt                                                                                                         <  1:1383404/141‑1 (MQ=255)

                                    ttATAGTAAAGTCAAAGAAATAATAGCAAAGAGTTATATATAAGATAAAGATATGCAA‑TTAGACTCTTATTGTCGGTTCAAGAATGTTATAAAAAAAAAAAAAAAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTg                                                                                                        <  2:665979/141‑1 (MQ=255)

                                      aTGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGaa                                                                                                      >  2:812427/1‑141 (MQ=255)

                                      aTGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGaa                                                                                                      >  2:556486/1‑141 (MQ=255)

                                       tGGTGAAGTCAACGAAATTATGGCAATAAGTTAGATTTATGATAAAGATATGCAA‑ATAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAAATAAAAAAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGaaa                                                                                                     <  4:545671/141‑1 (MQ=255)

                                            aaGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAgtatg                                                                                                <  2:187046/141‑1 (MQ=255)

                                             aGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAgtatgt                                                                                               >  3:360767/1‑141 (MQ=255)

                                              gTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAgtatgtt                                                                                              >  2:684870/1‑140 (MQ=255)

                                              gTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAgtatgta                                                                                              <  4:390201/141‑1 (MQ=255)

                                                cAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATc                                                                                            <  1:1021508/141‑1 (MQ=255)

                                                 aaCGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATcc                                                                                           <  2:1417985/141‑1 (MQ=255)

                                                   cGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAAATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCat                                                                                         <  2:244463/141‑1 (MQ=255)

                                                   cGAAATTATGGCAATAAGTTATATTTATGATAAAGATATACAA‑TTAGACTCTTAGCGTCTTTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCat                                                                                         <  2:1096248/141‑1 (MQ=255)

                                                       aTTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATTTATCTataata                                                                                     >  3:404229/1‑141 (MQ=255)

                                                       aTTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCataata                                                                                     >  1:288066/1‑141 (MQ=255)

                                                        ttATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAatat                                                                                    <  1:808859/141‑1 (MQ=255)

                                                         tATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATAtt                                                                                   >  4:68402/1‑141 (MQ=255)

                                                          aTGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATta                                                                                  >  2:325088/1‑141 (MQ=255)

                                                          aTGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATta                                                                                  >  2:135193/1‑141 (MQ=255)

                                                          aTGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATta                                                                                  >  1:558845/1‑141 (MQ=255)

                                                           tGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATtaa                                                                                 >  1:1093350/1‑141 (MQ=255)

                                                           tGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATtaa                                                                                 <  2:799487/141‑1 (MQ=255)

                                                               aaTAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AAAGTGATAAAAAAACTAAGTGAAAGTATGTATCCATAATATTAATaaa                                                                             >  1:443365/1‑141 (MQ=255)

                                                                aTAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATaaaa                                                                            <  1:812427/141‑1 (MQ=255)

                                                                  aaGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAat                                                                          <  4:636301/141‑1 (MQ=255)

                                                                   aGTTATATTTATGATAAAGATATGCAATTTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAat                                                                          <  3:194367/141‑1 (MQ=255)

                                                                     ttATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCataat                                                                                      <  1:1148132/126‑1 (MQ=255)

                                                                        tatTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAg                                                                    >  2:1070681/1‑141 (MQ=255)

                                                                          tttATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTa                                                                  >  2:666830/1‑141 (MQ=255)

                                                                            tATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑TAAATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTaa                                                                 <  1:654841/141‑1 (MQ=255)

                                                                              tGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACac                                                              <  1:1573993/141‑2 (MQ=255)

                                                                              tGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAg                                                              >  2:1027950/1‑141 (MQ=255)

                                                                               gATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGc                                                             <  1:770257/141‑1 (MQ=255)

                                                                                   aaGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCAttt                                                         <  1:770004/141‑1 (MQ=255)

                                                                                   aaGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCAttt                                                         <  1:396273/141‑1 (MQ=255)

                                                                                     gATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTtg                                                       <  1:1314810/141‑1 (MQ=255)

                                                                                      atatGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTtga                                                      <  1:711203/141‑1 (MQ=255)

                                                                                          gCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTtgaatga                                                  >  3:625896/1‑141 (MQ=255)

                                                                                             a‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAg                                               >  2:300453/1‑141 (MQ=255)

                                                                                             a‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAg                                               <  1:667207/141‑1 (MQ=255)

                                                                                             a‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAg                                               >  2:2155/1‑141 (MQ=255)

                                                                                                  gACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTGAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGt                                           >  4:675816/1‑141 (MQ=255)

                                                                                                   aCTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTc                                          <  3:69199/141‑1 (MQ=255)

                                                                                                   aCTCTTATCGTCGGTTCAAGAATGTTATAAAAAAAATAAAAAAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTc                                          <  4:431578/141‑1 (MQ=255)

                                                                                                    ctctTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCt                                         <  2:476132/141‑1 (MQ=255)

                                                                                                    ctctTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCt                                         <  1:1160779/141‑1 (MQ=255)

                                                                                                    ctctTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCt                                         >  2:860112/1‑141 (MQ=255)

                                                                                                     tctGATCGTCGGTTCAAGAATGTTATAAATCATATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGGATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCtt                                        <  2:53940/141‑1 (MQ=255)

                                                                                                       ttATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTa                                      <  1:1435410/141‑1 (MQ=255)

                                                                                                       ttATCGTCGGTTCAAGAATGTTATAAATCAGATAAAAGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTa                                      <  2:249187/141‑1 (MQ=255)

                                                                                                        tATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTAt                                     <  3:43510/141‑1 (MQ=255)

                                                                                                          tcgtcgGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTg                                   <  2:715495/141‑1 (MQ=255)

                                                                                                            gtcgGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGtt                                 >  2:785080/1‑141 (MQ=255)

                                                                                                              cgGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCa                               <  1:1581443/141‑1 (MQ=255)

                                                                                                              cgGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCa                               >  2:1304918/1‑141 (MQ=255)

                                                                                                                gTTCAAGAATGTTATAAATCAGATAAATGAAAAGAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAAt                             >  2:164458/1‑141 (MQ=255)

                                                                                                                gTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAAt                             >  2:1099805/1‑141 (MQ=255)

                                                                                                                gTTAAAGAATGTTATAAAATAGATAAATAAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATACGTAATATTAATTAACTTATACCGTAACAGTATTTTGCATGACAGATCTCTGTATTGGTCAAt                             <  2:978060/141‑1 (MQ=255)

                                                                                                                  tCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTATT‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATc                            >  4:42587/1‑141 (MQ=255)

                                                                                                                  tCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCa                           <  3:249713/141‑1 (MQ=255)

                                                                                                                  tCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGAGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCa                           >  3:578170/1‑141 (MQ=255)

                                                                                                                   cAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCat                          >  1:1430664/1‑141 (MQ=255)

                                                                                                                      gAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCattta                       >  2:1210774/1‑141 (MQ=255)

                                                                                                                      gAATGTTATAAATCAGATAAATAAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCattta                       >  1:1268163/1‑141 (MQ=255)

                                                                                                                       aaTGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCatttat                      <  2:63469/141‑1 (MQ=255)

                                                                                                                          gTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTAtttt                   >  1:1110511/1‑141 (MQ=255)

                                                                                                                          gTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTAtttt                   >  4:632349/1‑141 (MQ=255)

                                                                                                                           ttATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTa                  >  1:527546/1‑141 (MQ=255)

                                                                                                                           ttATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTa                  >  1:1527730/1‑141 (MQ=255)

                                                                                                                               aaaTCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTaa              <  3:264733/141‑1 (MQ=255)

                                                                                                                                 ataagaaaaaagaaaaaAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATg            <  4:650093/138‑1 (MQ=255)

                                                                                                                                     gATAAATGAAAAAAACACTTTA‑T‑AATGTGATGAATAAACGAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTc        >  2:909223/1‑141 (MQ=255)

                                                                                                                                     gATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTc        <  1:487467/141‑1 (MQ=255)

                                                                                                                                      aTAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTCa       >  4:357269/1‑141 (MQ=255)

                                                                                                                                       tAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTCaa      <  2:1277853/141‑1 (MQ=255)

                                                                                                                                         aaTGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTCAAAt    >  4:494579/1‑141 (MQ=255)

TACTGTTCAACAAAAAATTCAAGATTTTATGGAAACTTATGGTGAAGTCAACGAAATTATGGCAATAAGTTATATTTATGATAAAGATATGCAA‑TTAGACTCTTATCGTCGGTTCAAGAATGTTATAAATCAGATAAATGAAAAAAACACTTTA‑T‑AATGTGATAAATAAACTAAGTGAAAGTATGTATCCATAATATTAATAAAAATATACAGTAACAGCATTTTGAATGAAAGATGTCTTTATTGTTCAATCATTTATTTTAGTAATGATTCAAATTC  >  USA300TCH1516_ALE/1732658‑1732936

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`