BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	2,774,855	A→G	6.0%	D777D (GAT→GAC)	clfB ←	Clumping factor B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	2,774,855	0	A	G	6.0%	510.7 / 3.1	199	D777D (GAT→GAC)	clfB	Clumping factor B
Reads supporting (aligned to +/- strand): ref base A (94/88); new base G (5/9); total (101/98)
Fisher's exact test for biased strand distribution p-value = 2.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 202 total aligned reads displayed.

AGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATC  >  USA300TCH1516_ALE/2774716‑2774995

aGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAAt                                                                                                                                             >  1:1174282/1‑141 (MQ=255)

  tCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATcc                                                                                                                                           >  2:939250/1‑141 (MQ=255)

   cACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGGCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCg                                                                                                                                          >  1:1225388/1‑141 (MQ=255)

     cTATCTGAGTCTGAGCCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGaa                                                                                                                                        >  1:769088/1‑141 (MQ=255)

        tctgagtctgagTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCg                                                                                                                                     >  1:1430531/1‑141 (MQ=255)

        tctgagtctgagTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCg                                                                                                                                     >  1:522848/1‑141 (MQ=255)

         ctgagtctgagtCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCCGACTGAGTCGGAGTCGCTGTCTGCGTCCGAATCGc                                                                                                                                    >  2:496280/1‑141 (MQ=14)

           gagtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                                                                                                                                  >  3:195728/1‑141 (MQ=255)

           gagtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                                                                                                                                  >  1:684853/1‑141 (MQ=255)

           gagtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTa                                                                                                                                  >  1:609850/1‑141 (MQ=255)

           gagtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGCCTGGGTCGCTATCCGATTCTGAGTCGCTGTCTGACTCTGAATCGCTGTCTGATTCCGAATCGcaa                                                                                                                                  >  2:154619/1‑139 (MQ=17)

             gtctgagtcGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCTGAATCGCTatc                                                                                                                                >  1:723133/1‑141 (MQ=255)

             gtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTGGCTGTCTGTATCTGATTGGCTGTCTGTATCTGAGTGGCTatt                                                                                                                                >  2:474350/1‑140 (MQ=11)

             gtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatc                                                                                                                                <  3:597115/141‑1 (MQ=255)

             gtctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatc                                                                                                                                <  2:1228910/141‑1 (MQ=255)

              tctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGATTCTGATTCACTGTCTGAATCTGAATCGCTatct                                                                                                                               >  2:147155/1‑141 (MQ=255)

               ctgagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctg                                                                                                                              >  4:573579/1‑141 (MQ=255)

                 gagtcGCTGTCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                                            <  4:109453/141‑1 (MQ=255)

                 gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                                            >  3:369894/1‑141 (MQ=255)

                 gagtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaa                                                                                                                            <  1:49757/141‑1 (MQ=255)

                  agtcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaat                                                                                                                           <  1:1342230/141‑1 (MQ=255)

                    tcGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatct                                                                                                                         <  1:158920/141‑1 (MQ=255)

                     cGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctg                                                                                                                        <  1:696904/141‑1 (MQ=255)

                      gCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctgaatctga                                                                                                                       <  1:556562/141‑1 (MQ=255)

                          tCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGCGTCTGAGTCGCTGTCTGAGTCTGAATCTCTGTCTGAATCTGAATCGCTATCTGAATCTGAATCg                                                                                                                   >  2:794727/1‑141 (MQ=255)

                           cTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                                  <  2:727929/141‑1 (MQ=255)

                           cTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGc                                                                                                                  <  3:146342/141‑1 (MQ=255)

                                 ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                            >  1:544336/1‑141 (MQ=255)

                                 ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                            >  2:1504096/1‑141 (MQ=255)

                                 ccGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCg                                                                                                            <  1:2292/141‑1 (MQ=255)

                                     gTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTc                                                                                                        <  1:31541/141‑1 (MQ=255)

                                     gTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTc                                                                                                        <  2:1441802/141‑1 (MQ=255)

                                      tCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCACTGTCTGAATCCGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCt                                                                                                       >  4:42367/1‑141 (MQ=255)

                                       cGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTg                                                                                                      <  3:446941/141‑1 (MQ=255)

                                         ccaTCCGTGGCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCGGAGTCGCTGTCTGAATCTGAATCGCTGGCTGAGTCCGAATCGCTATCTGAATCTGACGCGCTATCCGAGTCTGAg                                                                                                    <  2:1199917/139‑1 (MQ=17)

                                         cTATCCGGGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                                    <  4:258419/141‑1 (MQ=255)

                                            tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGGTGTCCGAGTCCGACTCGATATCTGAATCTGGGTCGTTGGCCTAGTCTGaaacg                                                                                                 >  4:333313/1‑137 (MQ=11)

                                            tCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAATCGCTGTCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCg                                                                                                 >  2:240622/1‑141 (MQ=14)

                                            cccGAGACTGAGTCGCTATCTGAGGCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGGATCTGCCTCGCTGTCTGACTCGGAGTCGCTGTCCGAGTATGAGTCg                                                                                                 >  2:55474/2‑141 (MQ=14)

                                             tcGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                <  2:1392259/140‑1 (MQ=255)

                                             ccGTGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                <  2:417638/141‑1 (MQ=255)

                                             ccGAGTCTGAGTCGCTATCTGAGTCGGAGTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                <  2:75520/141‑1 (MQ=255)

                                               gAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                                                                              <  1:1125494/141‑1 (MQ=255)

                                               gAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTg                                                                                              >  1:437846/1‑141 (MQ=255)

                                                  tCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtct                                                                                           <  4:385935/141‑1 (MQ=255)

                                                  tCTGAGGTGCCATCTGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAGTCTGAATCGCTATCCGAGTCTGAGTCGCTGtct                                                                                           <  2:1077060/141‑1 (MQ=11)

                                                           cTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgag                                                                                  >  2:1167372/1‑140 (MQ=255)

                                                           cTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGtctgaatctgaa                                                                                  <  1:362632/141‑1 (MQ=255)

                                                              tCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTa                                                                                                                <  4:492704/108‑1 (MQ=255)

                                                                   gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                <  2:421533/119‑1 (MQ=255)

                                                                   gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGc                                                                                                >  1:421533/1‑119 (MQ=255)

                                                                   gTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTc                                                                          >  2:914355/1‑141 (MQ=255)

                                                                       gAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTATCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAg                                                                      <  4:186103/141‑1 (MQ=255)

                                                                         aTCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCTGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCGGAGTc                                                                    >  4:273143/1‑141 (MQ=255)

                                                                          tCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATATGAATCGCTCTCCGGGTCTGATGGGCTTTCTGAATCTGAATGGCTGTCTGAGTcc                                                                   >  1:1151827/1‑141 (MQ=18)

                                                                            gCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGGATCGGAATCGCTTTCCGAGTCTGACTCGCCGTCTGACTCTGAATCGCTGTCTGAGTACGa                                                                 >  2:677066/1‑141 (MQ=17)

                                                                             cTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGaa                                                                <  1:203432/141‑1 (MQ=255)

                                                                                tctgagtctgagTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                                    >  4:676341/1‑102 (MQ=255)

                                                                                tctgagtctgagTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                                    <  3:676341/102‑1 (MQ=255)

                                                                                  tgagtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCt                                                           >  3:20964/1‑141 (MQ=255)

                                                                                     gtctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatc                                                        <  2:1035180/141‑1 (MQ=255)

                                                                                      tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAAACTGAATCGCCGTCTGAGTCCGAAGCGCTatcg                                                       >  2:20854/1‑140 (MQ=255)

                                                                                      tctgagtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatct                                                       >  1:1326706/1‑141 (MQ=255)

                                                                                           gtcGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTatctga                                                     <  1:1255976/138‑1 (MQ=255)

                                                                                               cTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                              <  2:1466903/141‑1 (MQ=255)

                                                                                                 aTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTatctg                                                                                                                              <  3:334859/59‑1 (MQ=255)

                                                                                                    cGAGTCTGAGTCGCTGTCTGAATCTGAGTCACTGTCTGAATCTGAATCGCTGTCTGAATCTGAGTCCCTATCTGAGTCTGAGTGGTTGTCGGAGTCTGAATAACGGTAAGAGTCTGAATTGCTCTCTAAATCTGAGTCgac                                         >  2:1385717/1‑139 (MQ=2)

                                                                                                     gtatCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGATTCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                        <  2:288174/138‑1 (MQ=255)

                                                                                                     gAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTg                                        <  3:534853/141‑1 (MQ=255)

                                                                                                      aGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCGCTCTCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgt                                       <  2:806807/141‑1 (MQ=255)

                                                                                                        tCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGATTCCGAATCGCTATCTGAATCTGAGTCGCTgtct                                     <  1:865933/141‑1 (MQ=255)

                                                                                                          tGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctga                                   <  1:914355/141‑1 (MQ=255)

                                                                                                           gAGTCGCTGTCTGAATCTGAATCACTGTCTGAGTCCGAAGCGCGATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTGTCTGATTCTGAATCGCTGTCTGAGTCCGAGTCGCTGTCTGAATCTGAGTCGCTctctgag                                  >  2:1138174/1‑141 (MQ=11)

                                                                                                           gAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTgtctgag                                  >  1:511749/1‑141 (MQ=255)

                                                                                                                 cTGTCTGAATCTGAATCGCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGaa                            <  2:39577/141‑1 (MQ=255)

                                                                                                                    tctgaatctgaaTCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCg                         >  3:484762/1‑141 (MQ=255)

                                                                                                                      tgaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAg                                              <  4:670937/118‑1 (MQ=255)

                                                                                                                       gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa                      <  4:579277/141‑1 (MQ=255)

                                                                                                                       gaatctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTa                      <  4:241052/141‑1 (MQ=255)

                                                                                                                         atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatc                    <  1:1204322/141‑1 (MQ=255)

                                                                                                                         atctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTATCTGAGTCCGAATCGCTATCTGAATCTGAGTGGCTGTCCGACGCTGAGTCGCtgtg                    >  2:841208/1‑138 (MQ=17)

                                                                                                                          tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatct                   <  2:94108/141‑1 (MQ=255)

                                                                                                                          tctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatct                   >  1:182783/1‑141 (MQ=255)

                                                                                                                           ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                                  >  4:398031/1‑93 (MQ=255)

                                                                                                                           ctgaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCg                                                                  <  3:398031/93‑1 (MQ=255)

                                                                                                                             gaatcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaa                >  1:140888/1‑141 (MQ=255)

                                                                                                                               atcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatc              >  2:821291/1‑141 (MQ=255)

                                                                                                                                tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                                    <  1:1635578/54‑1 (MQ=255)

                                                                                                                                tcACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAg                                                                                                    >  2:1635578/1‑54 (MQ=255)

                                                                                                                                  aCTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTatctgaatctga           >  1:284709/1‑141 (MQ=255)

                                                                                                                                   cTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATTTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg          >  1:439068/1‑141 (MQ=255)

                                                                                                                                   cTGTCTGAATCCGAATCGCTATCTGAATCTGAAGCGCTATCCGTTTGTGAGTGGCGTTCTGAAGTGTAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAg          <  1:151528/141‑1 (MQ=14)

                                                                                                                                     gTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAGTCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc        <  2:42565/141‑1 (MQ=255)

                                                                                                                                     gTCTGAATCCGAATCGCTATCTGATTCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTc        <  2:1346340/141‑1 (MQ=255)

                                                                                                                                        tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAGTCTGAGTCGCTGTCTGAGTCCGACTCGCTATCTGAATCTGAGTCGc                                          <  2:644345/104‑1 (MQ=255)

                                                                                                                                        tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAGTCGCt     >  4:337346/1‑141 (MQ=14)

                                                                                                                                        tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGc                                          >  1:644345/1‑104 (MQ=255)

                                                                                                                                        tGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCt     >  1:1129524/1‑141 (MQ=255)

                                                                                                                                          aaTCCGAATCGCTATCTGAATCTGAATCGCGATCCGAGTCGTAGGCGCTGTCTGAGTCTGAATCGCTGTCTGAGTCCGAATCTCTATCTGAATCTGATTCGCTAGCTGATACTGTTTCTCTAGCTGTATCTCANTCGCTAt   >  2:1242187/1‑141 (MQ=14)

                                                                                                                                           aTCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGGCGCTGTCTGAGTCTGAGTCGCTGTCTGAATCTGAGACGCtgta  >  2:570698/1‑138 (MQ=14)

AGTCACTATCTGAGTCTGAGTCGCTATCTGAATCCGAGTCGCTATCCGAGTCTGAGTCGCTATCTGAGTCTGAATCGCTGTCTGAGTCTGAGTCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCACTGTCTGAATCCGAATCGCTATCTGAATCTGAATCGCTATCCGAGTCTGAGTCGCTGTCTGAATCTGAATCGCTGTCTGAGTCCGAATCGCTATCTGAATCTGAGTCGCTGTCTGAGTCTGAATCGCTATCTGAATCTGAGTCGCTATC  >  USA300TCH1516_ALE/2774716‑2774995

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`