Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 613,998 G→A 5.8% S770S (TCG→TCA sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE613,9980GA 5.8% 887.4 / 3.8 392S770S (TCG→TCAsdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base G (165/172);  new base A (40/4);  total (208/184)
Fisher's exact test for biased strand distribution p-value = 3.09e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01

Only 100 of 397 total aligned reads displayed.

GCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCT  >  USA300TCH1516_ALE/613865‑614130
                                                                                                                                       |                                                                                                                                     
gCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCg                                                                                                                                       >  1:2726018/1‑134 (MQ=255)
 ggATTCAGACGGAGATAGAGGCTCATGTTCAGATAGTGACTCAGATGCAGACAGCGATTCAGACAGTGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCgg                                                                                                                                      <  2:1238900/133‑1 (MQ=18)
  gATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGa                                                                                                                                     <  1:884516/134‑1 (MQ=255)
    ttCAGACTCAGATAGCGACTCAGATTCAGGTACAATACAAATGGCTGACAGCGAGTCCGACTGAGACTGCGACTCAGATAACGATTCAGATTGAAACAGCGACTAACACTCAAAGAGCCATGCTGACTCGGaag                                                                                                                                   >  2:1312935/1‑132 (MQ=255)
    ttCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAACGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATa                                                                                                                                   >  1:1314521/1‑134 (MQ=255)
      taGACTCAGAGAGCGACTCAGAGTCAGATAGCGACTCAGATGCAGACCGCGATTCGGACAGCCATCCACCCTCAGATATCGATTCAGATTCTGACAGCGACTCAGACTCAGACATCGATTCAGACTCGGATAGc                                                                                                                                 <  2:38329/133‑1 (MQ=18)
      cAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACATTGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGc                                                                                                                                 <  1:1040069/134‑1 (MQ=255)
      cAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACACAGACTCCGACTCAGCTAGCATTTCCGCTTCGGACACAGACTCAGCCTCAGACACAGCTCCAGACTGAGATAga                                                                                                                                 >  2:1172437/1‑133 (MQ=9)
      cAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAAAGACACAGACTCAGATAGAGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGc                                                                                                                                 >  1:914842/1‑134 (MQ=18)
       aGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCg                                                                                                                                >  1:2038554/1‑134 (MQ=255)
        gtttcagataggtactcaggtttagatagagacgcaggtTCAGAAAGCGATATAGACAGCGATTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGAGTCAGACTGCGATTCAGAATCGGGTAGCga                                                                                                                               <  2:1111343/131‑1 (MQ=17)
        gACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCga                                                                                                                               >  1:489687/1‑134 (MQ=255)
         aCTCAGATAGCGACTCAAATTCACATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgac                                                                                                                              <  1:1123567/134‑1 (MQ=255)
           tcagatagcgactcagattcagatagcgactcagatTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactc                                                                                                                            >  1:2002274/1‑134 (MQ=255)
            cagatagcgactcagattcagatagcgactcagattCAGACAGCGATTTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactc                                                                                                                            >  1:2605137/1‑134 (MQ=255)
               atagcgactcagattcagatagcgactcagattcagACAGCTATTCAGACAGCGACTCAGACTCAGATAGCGATACAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagac                                                                                                                        <  1:1837396/134‑1 (MQ=255)
                tagcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGAGACTCAGAATAAGACAGTGATTCAGAGCCCGATAGAgacccagaga                                                                                                                       >  2:2269954/1‑132 (MQ=18)
                 agcgactcagattcagatagcgcctcagattcagaTAGCGATTTAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagactc                                                                                                                      <  1:2317614/134‑1 (MQ=255)
                 agcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACCGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCgactcagactc                                                                                                                      >  1:470959/1‑134 (MQ=255)
                 agcgactcagattcagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGACTCAGATTCAGACAGCGACTCAGACTCAGACACCGATTCCGACTCAGATCGCcactcagcctc                                                                                                                      >  2:523780/1‑134 (MQ=18)
                    gacgcagaatcagatagcgacgcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGActcagactcaga                                                                                                                   <  2:2453914/134‑1 (MQ=255)
                      ctcagattcagatagcgactcagattcagaCAGCGATTCAGACACCGACTCAGACTCAGATGGCGATTCAGATTCAGACAGCGATTCAGACGCAGACACCGACTCAGACTCCGATACAGAATCAGACTCCGAtc                                                                                                                 >  2:605982/1‑133 (MQ=18)
                              cagatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCa                                                                                                         <  1:1533534/134‑1 (MQ=255)
                                gatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACGCAGACTCAGACAGCGAcaccgc                                                                                                       >  2:2087739/1‑129 (MQ=18)
                                gatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGAGATACAGCTTCAGACAGCGACTGAGACTCAGACAGAGATTAAGACTCGGGTAGCGACTGAGGCTCAGAGAGAGAATCAGa                                                                                                       >  2:1061625/1‑134 (MQ=18)
                                gatagcgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAACGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGa                                                                                                       >  1:522709/1‑134 (MQ=255)
                                 cttgcgactcagattcagaCAGCGATTCAGACAGCGACTCTGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGAt                                                                                                      <  2:1384507/131‑1 (MQ=255)
                                     cgactcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGACAGCGACTCAGACTAAGCTTAGGACACAGATTCgt                                                                                                  >  2:1687307/1‑133 (MQ=18)
                                          cagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGc                                                                                             <  1:1875230/134‑1 (MQ=255)
                                          cagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGc                                                                                             >  2:883895/1‑134 (MQ=255)
                                           agattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCg                                                                                            >  1:420916/1‑134 (MQ=255)
                                            gattgagacagcgagtgagacagcGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCga                                                                                           <  2:1212718/134‑1 (MQ=255)
                                            gattcagacagcgattcagacagcGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCga                                                                                           <  1:135976/134‑1 (MQ=255)
                                                 cagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgactca                                                                                       >  1:2653704/1‑134 (MQ=255)
                                                   gacaacgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgactcaga                                                                                     <  2:2576548/134‑1 (MQ=255)
                                                   gacaacgatgcaaacagagaCTCAGACTAAGATAGCGATTCAGATTAAGACATCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgactcaga                                                                                     <  2:968576/134‑1 (MQ=17)
                                                             tcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAg                                                                           >  1:2745576/1‑134 (MQ=255)
                                                              ttgacagggaCTAAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGc                                                                          <  2:2524379/132‑1 (MQ=255)
                                                              cagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCGGATAGCGTCTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGc                                                                          <  1:181682/134‑1 (MQ=255)
                                                                 acagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgat                                                                       <  1:1270216/134‑1 (MQ=255)
                                                                   agtgactgagactcaTATAGCGATTCAGATTCAGACAGTGACTCAGACTCATACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattc                                                                     <  2:363951/131‑1 (MQ=17)
                                                                   agcgaCTCAGACTCAGATATCGAGTCAGATTCAGACAGCGACTCAGACTTAGACAGCGATTCAGACTCGGATAGCGATTCAGACTCAGATAGCGACGCAGATGCGGATAGCGACTCAGACTCAGATAGCgattc                                                                     <  1:750055/134‑1 (MQ=17)
                                                                    gcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAGTCAGATTCAGAAAGCGACTCAGACTCAGACAGCgcatca                                                                    >  2:1943884/1‑129 (MQ=14)
                                                                       actcagactcagATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGAGTCGGATAGCGACTCAGACTCAGATAGCgatacagaa                                                                 >  2:437261/1‑133 (MQ=255)
                                                                         tcagactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGAGAATCAGACTCAGATAGCgattcagattc                                                               >  1:392355/1‑134 (MQ=255)
                                                                            gactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGAttcagattcaga                                                            >  1:1456614/1‑134 (MQ=255)
                                                                            gactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGAttcagattcaga                                                            >  1:288980/1‑134 (MQ=255)
                                                                            gactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGCctaagactcaga                                                            >  2:2653524/1‑134 (MQ=14)
                                                                               tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                                         >  1:819876/1‑134 (MQ=255)
                                                                                 agaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCg                                                       <  1:988223/134‑1 (MQ=255)
                                                                                 agaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCCGCTCGAAATAGCGATtcggattcagattgtg                                                       >  2:1969071/1‑130 (MQ=14)
                                                                                  gaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGa                                                      >  1:2442770/1‑134 (MQ=255)
                                                                                     aGCGATTCAGATTCAGACAGCGTCTCATATTCAGATAGTGATTCAGACTCCGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTc                                                   <  1:2553339/134‑1 (MQ=14)
                                                                                      gCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGAGACTAAGACTCAGATATCTAATAAGAATCACATAGCGaaaca                                                  >  2:922535/1‑130 (MQ=14)
                                                                                      gCGATGCAGATTCAGACAGCGACCCAAACCCATCCAGCGCCTAATTTTGGGATGGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCg                                                  <  2:1725900/134‑1 (MQ=14)
                                                                                       cGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCgg                                                 >  1:2531385/1‑134 (MQ=255)
                                                                                       cGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCgg                                                 <  1:1752539/134‑1 (MQ=255)
                                                                                        gattcagattcaGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGAGTCGAAGCCAAATAGCGATTCAGATTCAGATAGCGATTCggc                                                >  1:1958004/1‑133 (MQ=17)
                                                                                         attgagattcagATAGTGACTATGAGTCAGACTGCGATTCTGACTCGGATAGCGATTCAGACTCAGGTTGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGAc                                               <  2:801332/134‑1 (MQ=17)
                                                                                          ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACt                                              >  1:277451/1‑134 (MQ=255)
                                                                                          ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACt                                              >  2:643242/1‑134 (MQ=255)
                                                                                           tcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTc                                             <  1:1660489/134‑1 (MQ=255)
                                                                                           tcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACCCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTc                                             >  1:1495215/1‑134 (MQ=255)
                                                                                              gattcagaCAGGTACGAAGACTCAGACAGAGATTCAGACTCTGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGa                                          <  2:2198214/134‑1 (MQ=14)
                                                                                              gattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGAATCAGAGTCAGATAACCATTCGGACACAGa                                          >  2:478680/1‑134 (MQ=14)
                                                                                              gattcagaAAGCGACGCGGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGa                                          <  2:1835834/134‑1 (MQ=255)
                                                                                               attcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGACTCGGACTCAGAc                                         >  1:1608438/1‑134 (MQ=255)
                                                                                               attcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGAc                                         >  1:2480099/1‑134 (MQ=255)
                                                                                               attcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACACAGACTCAGATATCGACTCAGATTCAGCGTGTGATTAAGACTCAGAc                                         >  2:2144094/1‑134 (MQ=11)
                                                                                                ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATACCGATTCAGATTCAGATAACGAATCGGACTCCGAcg                                        >  2:302202/1‑133 (MQ=14)
                                                                                                 tcagaCAGCGACTCAGACTCAGACAGCGCTTCAGCCTCGGATAGCGACTCAGACTCAGATAGCGACTTGGTTTGGGGTGACTAATCAAAGTCAAAAAGAGATTCAGATTCAGATCGCGATTCGGACTAAGACAg                                       >  1:668231/1‑134 (MQ=9)
                                                                                                 gcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGATTCAGACTCAGATAGCGATTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAg                                       <  1:2236503/133‑1 (MQ=255)
                                                                                                  cagaCAGCGAGTCAGACGCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGt                                      <  2:1993341/134‑1 (MQ=255)
                                                                                                   agaCAGCGACTCCGCCTCAGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                     <  2:687773/134‑1 (MQ=255)
                                                                                                     aCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgat                                   >  1:2195178/1‑134 (MQ=255)
                                                                                                       aGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattc                                 <  1:2394426/134‑1 (MQ=255)
                                                                                                        gCGACTTAGACTCAGACAGCGATTCAGTCTCGGATAGCGTCTCAGACTCAGATAGCGACTCAGATTCGGATAGCGATTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattca                                <  1:2277423/134‑1 (MQ=255)
                                                                                                        gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattca                                >  1:1710868/1‑134 (MQ=255)
                                                                                                        gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGAATCAGAATCAGATAGCGATTCGGACTCAGACAGTgaatca                                >  2:1519334/1‑134 (MQ=255)
                                                                                                          tactcagactcagACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACACAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcaga                              <  2:2702954/133‑1 (MQ=255)
                                                                                                            ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagatt                            >  1:209105/1‑134 (MQ=255)
                                                                                                            ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCgattcagatt                            >  2:2415407/1‑134 (MQ=255)
                                                                                                            ctcagactcagaCAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGAgactcagctt                            >  2:2815105/1‑134 (MQ=14)
                                                                                                             tcagactcagacAGTGATTCAGACTCGGATAGTGACTGAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                           <  2:175854/134‑1 (MQ=255)
                                                                                                             tcagactcagacAGCGATTCAGACTCGGATATCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATACAGACCCAGATATCTATTCGGACTCAGACAGTgatccagattc                           <  1:1981517/134‑1 (MQ=17)
                                                                                                             tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                           <  1:2124765/134‑1 (MQ=255)
                                                                                                             tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                           <  1:1172437/134‑1 (MQ=255)
                                                                                                             tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGAGAGCGATTCAGCGTCAGATAGCGAATCGGACTCAGACAGAgaatcagattc                           >  2:2249440/1‑134 (MQ=17)
                                                                                                                      gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCCGACTCAGATAGCGATTCAGAATCAGATAACGAATCAAACTCAGACAGAGATACAGATTCAGACACAGa                  >  1:1008681/1‑134 (MQ=17)
                                                                                                                      gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGa                  <  1:1641766/134‑1 (MQ=255)
                                                                                                                        cAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATa                >  1:2686655/1‑134 (MQ=255)
                                                                                                                          tcGATTCAGACTCGGATAGCGACTCAGTCTCATATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGc              <  1:1217533/133‑1 (MQ=255)
                                                                                                                          gCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGc              >  1:1801002/1‑134 (MQ=255)
                                                                                                                            gATTCAGACTCGGATAGCGTCTCAGACTCAGATAGTGACTTAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGa            <  2:1560646/134‑1 (MQ=255)
                                                                                                                             gttCAGACTCGGATAGCGATTCAGTCTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc           <  2:1647100/133‑1 (MQ=255)
                                                                                                                               tCAGACTCGGATTCCGACTCAGACTCAGATAGCAACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTc         <  1:258990/134‑1 (MQ=255)
                                                                                                                                cAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTACGCGTCAGATAGCGACTCa        >  1:1074710/1‑134 (MQ=255)
                                                                                                                                 aGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGTATAGTGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAg       <  1:609663/134‑1 (MQ=255)
                                                                                                                                    cTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGAtt    <  1:726192/134‑1 (MQ=255)
                                                                                                                                      cGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCt  <  2:2372380/134‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                     
GCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCT  >  USA300TCH1516_ALE/613865‑614130

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: