Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 614,034 G→A 5.6% S782S (TCG→TCA)  sdrC → Serine‑aspartate repeat‑containing protein C

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE614,0340GA 5.6% 910.9 / 5.8 400S782S (TCG→TCA) sdrCSerine‑aspartate repeat‑containing protein C
Reads supporting (aligned to +/- strand):  ref base G (170/178);  new base A (36/5);  total (211/189)
Fisher's exact test for biased strand distribution p-value = 1.42e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 407 total aligned reads displayed.

GCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCA  >  USA300TCH1516_ALE/613901‑614166
                                                                                                                                     |                                                                                                                                    
    ctcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACACAGACTCAGATAGCGACAAAGACTCGGaaa                                                                                                                                  >  2:1728541/1‑132 (MQ=17)
    ctcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGACTCAGACAGCGACTCAGACTCAGACAGCGATTCCGACTCGGACACCGACCCAGAGTCAGATACCGACTCAGAATCGGATa                                                                                                                                  >  2:1284522/1‑134 (MQ=18)
     tcagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAg                                                                                                                                 >  1:2779319/1‑134 (MQ=255)
      cagattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGc                                                                                                                                >  2:883895/1‑134 (MQ=255)
       agattcagaCAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCg                                                                                                                               >  1:1555971/1‑134 (MQ=255)
         gttcagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGAGATACAGACGCAGATAGCGATTCAGACTCAGATAGCAACTCAGATTCAGACAGCgac                                                                                                                             >  2:2077970/2‑134 (MQ=14)
         attcagccagcgattcagacatcgACTCAGACTCAGATAGCGATTCAGATTCAGATATCTTCTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgac                                                                                                                             <  1:758800/134‑1 (MQ=17)
         attcagacagtgatttaatcagaaACTCACACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgac                                                                                                                             <  1:1083840/134‑1 (MQ=17)
            cagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAACGAAACAAAAATAGATGGCGACTCAGATTCGGATAGCgactca                                                                                                                          >  1:1220648/1‑134 (MQ=18)
            cagacagcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATACAGACTCAGATAGTGACTCAGATCCCGATACCGACTGAGATACAGACAGCgattca                                                                                                                          >  2:519232/1‑134 (MQ=14)
                 agcgattcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGAATCGGACAGCgacaccgactc                                                                                                                     >  2:2809961/1‑134 (MQ=14)
                  gctagtcaaacagcgaCTCACACTCCGACAGCGATTAAGATTCAGACAGCGACTCATATTCAGATAGCGATTCAGACTCGGATTGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCgactcagactca                                                                                                                    <  2:2120909/129‑1 (MQ=11)
                       tcagacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAg                                                                                                               <  1:2674536/134‑1 (MQ=255)
                          gacagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCga                                                                                                            >  1:275754/1‑134 (MQ=255)
                           acagcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGATAGTGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgat                                                                                                           <  1:363522/134‑1 (MQ=255)
                             agtgactgagactcaTATAGCGATTCAGATTCAGACAGTGACTCAGACTCATACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattc                                                                                                         <  2:363951/131‑1 (MQ=17)
                              gcgaCTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGAGTCAGATTCAGAAAGCGACTCAGACTCAGACAGCgcatca                                                                                                        >  2:1943884/1‑129 (MQ=14)
                              gcaactcagactcagATAGCGATTCAGATTCAGACAGCGACTCAGAATCATACAGCGATTCAGTCTCGAATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCgattca                                                                                                        <  1:1811292/131‑1 (MQ=17)
                                   tcagactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGAATCGGATAGAGACTCAGAATCAGATAGCgactcagatac                                                                                                   >  1:2410198/1‑132 (MQ=14)
                                     agactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGattcagattcag                                                                                                 >  1:2372380/1‑134 (MQ=255)
                                      gactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGAttcagattcaga                                                                                                >  1:1456614/1‑134 (MQ=255)
                                      gactcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGACAGCGCctaagactcaga                                                                                                >  2:2653524/1‑134 (MQ=14)
                                         tcagaTAGCGATTCAGATTCAGACAGCGACTCATTCTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                                                                             <  1:1972545/134‑1 (MQ=255)
                                         tcagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAg                                                                                             >  1:819876/1‑134 (MQ=255)
                                          cagaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGc                                                                                            >  1:2591719/1‑134 (MQ=255)
                                           agaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATGCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCg                                                                                           <  1:131557/134‑1 (MQ=255)
                                           agaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCg                                                                                           >  2:2587120/1‑134 (MQ=255)
                                           agaTAGCGATTCAGATGCAGTCAGCTACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGAAACAGAGTTTGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCg                                                                                           <  2:481043/134‑1 (MQ=17)
                                            gaTAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGa                                                                                          >  1:2442770/1‑134 (MQ=255)
                                              tAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCTGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGAtt                                                                                        <  1:883895/134‑1 (MQ=255)
                                                gCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGTGACTCATACTCAGATAGCGAATCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCg                                                                                      <  1:43575/134‑1 (MQ=255)
                                                 cGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCgg                                                                                     <  1:1752539/134‑1 (MQ=255)
                                                  gattcagattcaGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGAGTCGAAGCCAAATAGCGATTCAGATTCAGATAGCGATTCggc                                                                                    >  1:1958004/1‑133 (MQ=17)
                                                   attgagattcagATAGTGACTATGAGTCAGACTGCGATTCTGACTCGGATAGCGATTCAGACTCAGGTTGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGAc                                                                                   <  2:801332/134‑1 (MQ=17)
                                                    ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCCGACTGAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAACGACTCAGTGTCAAATAACAGTTCAGAGACAGGTAGACATACGGACt                                                                                  >  2:1697495/1‑134 (MQ=14)
                                                    ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACt                                                                                  >  2:643242/1‑134 (MQ=255)
                                                    ttcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACt                                                                                  >  2:1762653/1‑134 (MQ=255)
                                                     tcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTc                                                                                 <  1:1660489/134‑1 (MQ=255)
                                                     tcagattcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGACAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTc                                                                                 >  1:2568429/1‑134 (MQ=255)
                                                      cagattcagaTAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCa                                                                                <  1:2737812/134‑1 (MQ=255)
                                                         attcagaCAGCGACTCAGACTCAGACAGCGATTCAGAATCGGATAGCGACTCAGACTCAGATAGCGACGCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACCCAGAc                                                                             >  2:1119621/1‑134 (MQ=255)
                                                          ttcagaCAGCGACTCAGACTCAGACAGCGATTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGACTCAGACTCAGATACCGATTCAGATTCAGATAACGAATCGGACTCCGAcg                                                                            >  2:302202/1‑133 (MQ=14)
                                                           tcagaCAGCGACTCAGACTCAGACAGCAATTAAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAg                                                                           >  1:2090164/1‑134 (MQ=255)
                                                             agaCAGCGACTCCGCCTCAGACAGCGATTCAGACTCGGACAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTg                                                                         <  2:687773/134‑1 (MQ=255)
                                                             agaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTAAGATAGCGATTCGGACTCAGACAGTg                                                                         >  1:505402/1‑134 (MQ=255)
                                                              taacgCGACTCAGACTCATACCGCGATTAAGACTCGTATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTga                                                                        <  2:1227708/130‑1 (MQ=17)
                                                              gaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTga                                                                        <  1:2279396/134‑1 (MQ=255)
                                                              gaCAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTga                                                                        <  1:2001758/134‑1 (MQ=255)
                                                                 aGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGACTCAGAGTCAGATAGCGATTCGGACTCAGATAGCgattc                                                                     >  2:1980018/1‑134 (MQ=255)
                                                                  gCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattca                                                                    >  1:1710868/1‑134 (MQ=255)
                                                                    tactcagactcagACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACACAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcaga                                                                  <  2:2702954/133‑1 (MQ=255)
                                                                     actcagactcagACATCGATTCATTCTCTGATAGCGACTCAGACTAATATAGCGACTCAGAATCGGATAGCGACTCAGACTCAGATAGCGAGTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagat                                                                 <  1:250867/134‑1 (MQ=17)
                                                                     actcagactcagACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagat                                                                 <  1:921423/134‑1 (MQ=255)
                                                                      ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATGGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagatt                                                                >  1:2648398/1‑134 (MQ=255)
                                                                      ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCGGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCgatacagatt                                                                >  2:2370170/1‑134 (MQ=255)
                                                                      ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagatt                                                                >  1:1849746/1‑134 (MQ=255)
                                                                      ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCgattcagatt                                                                >  2:2415407/1‑134 (MQ=255)
                                                                      ctcagactcagaCAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCAGATAGCGATTCAGACTCAGACAGCgagtcagaat                                                                >  2:2461177/1‑132 (MQ=11)
                                                                       tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                                                               <  1:1172437/134‑1 (MQ=255)
                                                                       tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                                                               <  1:2124765/134‑1 (MQ=255)
                                                                       tcagactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGAGAGCGATTCAGCGTCAGATAGCGAATCGGACTCAGACAGAgaatcagattc                                                               >  2:2249440/1‑134 (MQ=17)
                                                                       gcagactcatatAGCGACTGAGGCTCAGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTgattcagattc                                                               <  2:1188547/133‑1 (MQ=14)
                                                                         agactcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGattcagattcag                                                             <  1:1784234/134‑1 (MQ=255)
                                                                           actcagacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACGCAGATAGCGATTCAGATGCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGAc                                                           <  1:1625/134‑1 (MQ=255)
                                                                                gacAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCCGACTCAGATAGCGATTCAGAATCAGATAACGAATCAAACTCAGACAGAGATACAGATTCAGACACAGa                                                      >  1:1008681/1‑134 (MQ=17)
                                                                                    gCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGc                                                  >  1:1525517/1‑134 (MQ=255)
                                                                                     cGATTCAGACTCGGATAGCTACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCTGGTTCGGATAACAATTCAGATTCAGATATCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCg                                                 <  1:676258/134‑1 (MQ=18)
                                                                                      gATTCGGACTCGGATAGCGACTCAGACTTAGATAGCGACTCAGATTCGGATTGCGACTCAGACTCAGAGAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCATATTCAGACTCAGATAGCGa                                                <  2:945368/134‑1 (MQ=18)
                                                                                       actCAGACTCGGATATCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                               <  2:400961/132‑1 (MQ=255)
                                                                                       aTTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGCCTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCATACAGTGATTCAGATTCAGACTCAGATAGCGAc                                               <  2:2536016/134‑1 (MQ=255)
                                                                                       aTTCAGACTCGGATAGCGACTCAGACTAAGATATCGACTCAGATTCTGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGAc                                               <  1:552892/134‑1 (MQ=255)
                                                                                         tCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTc                                             >  1:2552099/1‑134 (MQ=255)
                                                                                         tCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTCACATACCGAATc                                             >  2:1520319/1‑134 (MQ=255)
                                                                                         tCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGAGTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTc                                             <  1:619159/134‑1 (MQ=255)
                                                                                           aGACGCGGATAGCAACTCAGACTCAGATAGCGACTCAGATTCGGATAGCTACTCTGTCTCTGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAg                                           <  1:733075/134‑1 (MQ=18)
                                                                                                cGGATAGCGATGCAGCTTCAGATAGCGACGATGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACTGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCt                                      <  2:220291/134‑1 (MQ=18)
                                                                                                   agaGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGAc                                   <  2:1893137/132‑1 (MQ=255)
                                                                                                            cagactcagaTAGCGACTCAGATTCAGATAGCATATAAAACTCATATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtca                          <  1:2705302/134‑1 (MQ=18)
                                                                                                               actcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagac                       >  1:1241694/1‑134 (MQ=255)
                                                                                                               actcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagac                       <  1:643242/134‑1 (MQ=255)
                                                                                                                 tcagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGAAAGCGATtcagactc                     >  1:1294055/1‑134 (MQ=255)
                                                                                                                 tcagaGAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCTGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactc                     <  1:4764/134‑1 (MQ=255)
                                                                                                                  cagaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactca                    <  1:2250453/134‑1 (MQ=255)
                                                                                                                    gaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCCGATACCGACTCAGATTCTGACAGCGATtcagactcaga                  >  2:1059193/1‑134 (MQ=255)
                                                                                                                    gaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAATGATTCAGATTCAGACACAGATACAGATCCAGACTCAAGCAGCGAGtcacacgcaga                  >  2:438984/1‑134 (MQ=14)
                                                                                                                    gaTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATCCAGATAGCGATTCGGACTCAGACAGAGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATtcagactcaga                  >  1:1397026/1‑134 (MQ=255)
                                                                                                                     aTAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGACAGTGATTCAGATTCAGACTAAGATAGCGACTCAGATTCTGACAGCGATtcagactcagac                 >  1:1035130/1‑134 (MQ=255)
                                                                                                                      tAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGCGATTCAGATTCAGACAGCGATTCCGACTCAGATGCTCACACCAATACAGACTCAAACa                >  2:1636190/1‑134 (MQ=11)
                                                                                                                        gCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGTGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGc              <  1:2206360/134‑1 (MQ=255)
                                                                                                                        gCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGc              >  1:191614/1‑134 (MQ=255)
                                                                                                                           attCATATTCAGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgac           <  1:1061625/132‑1 (MQ=255)
                                                                                                                            cTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACACAGATAGAGACTCAGATTCTGACAGCGATTCAGACACAGACAGCgact          >  2:2262775/1‑134 (MQ=255)
                                                                                                                             ttaGATTCGGATAGCGATTAAGACTTAGATAGAGATTCAGATTCAGATAGAGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactc         <  2:1569507/132‑1 (MQ=21)
                                                                                                                               aGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACACCGACACAGACTCAGCTACCGACAGAGATTCAGACCCCGACCGCgactcac       >  2:1455140/1‑133 (MQ=14)
                                                                                                                                 aTTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgactcagac     <  1:1119550/134‑1 (MQ=255)
                                                                                                                                 aTTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGAATCAGACAGCGAGTCAGATTCAGACTCAGATAAAGACTCAGATACTGATATCGATTAAGAATCAGAAAGCaaaccagac     >  2:1999445/1‑134 (MQ=17)
                                                                                                                                 aTTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCAGACTCAGAAAATGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCgaatcagac     >  1:31444/1‑134 (MQ=255)
                                                                                                                                 aTTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGAGTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGAGACTCAGATACTGACAACGACTCAGAATCAGAAAGCgacgcaaac     >  2:132635/1‑134 (MQ=21)
                                                                                                                                 aTTCGGATAGCGACTCAGACTCAGATAGCGAATCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGAGACAGATACCGACTCAGATTCAGCCAGCCATTCAGAACCAAACAACcactaagac     >  2:62093/1‑134 (MQ=17)
                                                                                                                                   tCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTAAGACAGCgactcagactc   >  1:415855/1‑134 (MQ=255)
                                                                                                                                     |                                                                                                                                    
GCGACTCAGATTCAGACAGCGATTCAGACAGCGACTCAGACTCAGATAGCGATTCAGATTCAGACAGCGACTCAGACTCAGACAGCGATTCAGACTCGGATAGCGACTCAGACTCAGATAGCGACTCAGATTCGGATAGCGACTCAGACTCAGATAGCGATTCAGATTCAGATAGCGATTCGGACTCAGACAGTGATTCAGATTCAGACTCAGATAGCGACTCAGATTCTGACAGCGATTCAGACTCAGACAGCGACTCAGACTCA  >  USA300TCH1516_ALE/613901‑614166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑