Predicted mutation
evidence seq id position mutation freq annotation gene description
RA USA300TCH1516_ALE 848,908 G→T 21.5% E12* (GAA→TAA)  USA300HOU_RS04145 → Nucleotide‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*USA300TCH1516_ALE848,9080GT21.5% 353.5 / 80.6 204E12* (GAA→TAA) USA300HOU_RS04145Nucleotide‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (93/64);  new base T (25/20);  total (120/84)
Fisher's exact test for biased strand distribution p-value = 7.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

Only 100 of 206 total aligned reads displayed.

TCTAGACATAGTTGGAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGATGGAA  >  USA300TCH1516_ALE/848775‑849039
                                                                                                                                     |                                                                                                                                   
  tAGATATAGTTGGAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGaa                                                                                                                                   <  1:597883/134‑1 (MQ=255)
       aTAGTTGGAAATAAATATAAAACATTGTAGCTTAATGTTGGCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTAtt                                                                                                                              <  1:1954091/134‑1 (MQ=255)
       aTAGTTGGAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTAtt                                                                                                                              <  1:696415/134‑1 (MQ=255)
           ttGGAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCCTGAAATTAATTTTGGCGTTAAAAAATTAAAGTATAATAATGAAAAAAAAAAAAATAAAAGTGGAAtagtagat                                                                                                                          >  2:2036876/1‑132 (MQ=255)
            tGGAAATAAATATAAAACATCTTTGCTTAATTTTTTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTg                                                                                                                         <  1:1263801/134‑1 (MQ=255)
            tGGAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTGTGGAGATAAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTg                                                                                                                         <  2:653459/134‑1 (MQ=255)
             ggAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGt                                                                                                                        >  1:673609/1‑134 (MQ=255)
             ggAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGt                                                                                                                        >  1:475920/1‑134 (MQ=255)
             ggAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTAAACTATTAGTTGt                                                                                                                        >  2:283123/1‑134 (MQ=255)
              gAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTACCATCATGAATTTCGTTTTGGCGATGAAAAAAAAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTa                                                                                                                       >  1:953576/1‑134 (MQ=255)
               aaataaatATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTaa                                                                                                                      <  1:2354786/134‑1 (MQ=255)
                aataaataTAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAAc                                                                                                                     <  2:1480228/134‑1 (MQ=255)
                aataaataTAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAAc                                                                                                                     >  2:1250606/1‑134 (MQ=255)
                   aaataTAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAgg                                                                                                                  >  2:554350/1‑134 (MQ=255)
                    aataTAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGt                                                                                                                 >  2:947325/1‑134 (MQ=255)
                    aataTAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGt                                                                                                                 >  1:2141526/1‑134 (MQ=255)
                         aaaaCATCGTTGCTTAATTTTGTCATATAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATc                                                                                                            <  1:807516/134‑1 (MQ=255)
                          gaaCATTGTGTCTGAATTTTGTCATAGAAGATTTAAAGTAACATCAGGAAATTCGGTTTGGCGATGAAAAGATAATGGAGAATAATGAAAAAGAAAAAAGTATAAGTTAACTATTAGTTGTAACAGTTTTATCt                                                                                                           <  2:621489/133‑1 (MQ=255)
                          aatcATTGTTGCTTAATTTTGTCATAAAAAATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCt                                                                                                           <  1:2063247/131‑1 (MQ=255)
                           aaCATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTg                                                                                                          >  2:2116307/1‑134 (MQ=255)
                              aTCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATAAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTAACTGtcg                                                                                                       >  2:400935/1‑134 (MQ=255)
                               tCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGTTAATAATGATATAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGgcgc                                                                                                      <  1:1548272/134‑1 (MQ=255)
                                cGTTGCTTAATTTTGTCATCGAACATTGAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAAATATTAGTTGTAACAGGTTTATCTGGCGCa                                                                                                     >  1:2260286/1‑134 (MQ=255)
                                cGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTATAACAGGATTATCTGGCGCa                                                                                                     >  2:38160/1‑134 (MQ=255)
                                cGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATCACTAGTAACATGTTTATCTGTCGCa                                                                                                     >  2:1148032/1‑134 (MQ=255)
                                 gTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATAAGTTGTAACAGGTTTATCTGACGCAg                                                                                                    >  1:1132879/1‑134 (MQ=255)
                                 gTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATCAATTTCCCTCAGGCTGGGACATAATAATATATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAg                                                                                                    >  1:1971019/1‑134 (MQ=255)
                                  ttGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGAGATTTCTGGCGCAgg                                                                                                   >  2:316661/1‑134 (MQ=255)
                                   tGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGt                                                                                                  >  1:2577636/1‑134 (MQ=255)
                                   tGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGt                                                                                                  >  2:2735454/1‑134 (MQ=255)
                                    gCTTAATTTTGTAATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATTATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTa                                                                                                 <  1:1504494/134‑1 (MQ=255)
                                    gCTTAATTGTGTCATAGAATATTTAAATTAACATTATGAAATTAGTTTTTGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTa                                                                                                 <  2:2372841/134‑1 (MQ=255)
                                      ttAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAGAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTaaa                                                                                               >  1:2351494/1‑134 (MQ=255)
                                        aaTTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGAGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATc                                                                                             >  2:1906387/1‑134 (MQ=255)
                                        aaTTTTATCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGTTAAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTATTTGTAAAAGATTTATTTAACACAGGTAAATc                                                                                             >  2:751445/1‑134 (MQ=255)
                                          ttttGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAACGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTTGCGCAGGTAAATCtt                                                                                           >  2:1989303/1‑134 (MQ=255)
                                           tttGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCttt                                                                                          >  2:2494996/1‑134 (MQ=255)
                                            ttGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTg                                                                                         >  1:2809436/1‑134 (MQ=255)
                                              gTCATAGAACATTTAAATTAACATCATGAAATTCTTTTTGGGGGTTAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGt                                                                                       <  1:787164/134‑1 (MQ=255)
                                              gTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATCATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAATTGTAACAAGTTTAACAGGCGCAGGTAAATCTATGGt                                                                                       >  1:1295421/1‑134 (MQ=255)
                                               tCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAATTGTAACAGGTTTATCTGGAGCAGGTAAATCTTTGGtt                                                                                      >  1:1112851/1‑134 (MQ=255)
                                                  tAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCTGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTAtt                                                                                   <  1:2121346/134‑1 (MQ=255)
                                                   aGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTc                                                                                  >  2:531008/1‑134 (MQ=255)
                                                    gAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCa                                                                                 <  1:2448219/134‑1 (MQ=255)
                                                        atatAAATTAAAAACATGAAATACGTATTGGCGGTAAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGt                                                                             <  2:684398/131‑1 (MQ=255)
                                                        aTTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTTAATGTGAATTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGt                                                                             <  1:1092570/134‑1 (MQ=255)
                                                        aTTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGt                                                                             >  2:2287192/1‑134 (MQ=255)
                                                        aTTTAAATTAACATCATGAAATCGGTATTGACGTAGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGt                                                                             >  2:1755490/1‑134 (MQ=255)
                                                          ttAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGttt                                                                           >  2:1358371/1‑134 (MQ=255)
                                                           tAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTAAATGTTTa                                                                          >  1:2527752/1‑134 (MQ=255)
                                                            aaaTTAACATCATGAAATTCGTTTTGGCGGTGAAAAAAAAATGGATAATAATGAAACAGAAAAAAGTAAAAGTTAACTATTAGATGTAACATGTTTATCTGACCCAGGTACATCTTTGGTTATTCAATGTTTat                                                                         >  2:86964/1‑133 (MQ=255)
                                                             aaTTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTaga                                                                        >  2:1128253/1‑134 (MQ=255)
                                                              gttAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTagaa                                                                       <  2:2567235/133‑1 (MQ=255)
                                                              aTTGACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTagaa                                                                       <  2:1199663/134‑1 (MQ=255)
                                                                tAACATCATGAAATTCGTTTTGGAGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTATCAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTagaaga                                                                     <  2:1157654/134‑1 (MQ=255)
                                                                  aCATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACa                                                                   <  2:1462712/134‑1 (MQ=255)
                                                                  aCATCATGAAATTCGTTTTGGCGGTGAAAAAATAAAATATCATAATAAATATTAAAAAAGTAAAAGTGAACTATTAGGTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACa                                                                   <  1:2387396/134‑1 (MQ=255)
                                                                   catcatGATATTTGTTTTTGCGGTTTAAATGTAATTGTTTTTATTGTAATAGAAAAAAGTAAAAGTTAACTAGTAGTTGTAACAGGTTGATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACAt                                                                  <  1:2661702/134‑1 (MQ=255)
                                                                     tgttGAAATTCGGTATGGTGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATgg                                                                <  2:2359649/131‑1 (MQ=255)
                                                                        taaaaGTCGTTTTGGCAGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGat                                                             <  2:1685513/132‑1 (MQ=255)
                                                                           aaTTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATAtt                                                          <  1:77572/134‑1 (MQ=255)
                                                                            aTTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATAttt                                                         >  1:2733942/1‑134 (MQ=255)
                                                                             ttCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATAtttt                                                        >  1:1683497/1‑134 (MQ=255)
                                                                             ggcGTTTTGGCGGTGAAAAAATAATGGATAATAATTAAATAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATAtttt                                                        <  2:1946041/132‑1 (MQ=255)
                                                                             gacGTGTTGGAGGGGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGAAGGTCAGTCTTTGGTTATTCAATGTTTAGAAGACATGGGATAtttt                                                        <  2:1311888/132‑1 (MQ=255)
                                                                              tCGTTTTGGCGGTGAAAAAATAATGGATACTCCAGAAAGAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCCATGTTTAGAAGACATGTGTTAttttt                                                       >  2:200216/1‑134 (MQ=255)
                                                                              tCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATAttttt                                                       >  2:508340/1‑134 (MQ=255)
                                                                               cGTTTTGGCGTTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTtg                                                      <  1:2218615/134‑1 (MQ=255)
                                                                               cGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGACGACATGGAACATGTTtg                                                      >  2:1711507/1‑134 (MQ=255)
                                                                               cGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTtg                                                      >  2:2587233/1‑134 (MQ=255)
                                                                                gTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTATATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTtgt                                                     >  1:1815607/1‑134 (MQ=255)
                                                                                gTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTtgt                                                     >  1:2788712/1‑134 (MQ=255)
                                                                                gTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTtgt                                                     >  1:1214759/1‑134 (MQ=255)
                                                                                 ttttGGCGATGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTtgtg                                                    <  2:777626/134‑1 (MQ=255)
                                                                                     ggCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGa                                                >  1:900185/1‑134 (MQ=255)
                                                                                      gcagTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTAATAGATGTAACAGGTTTATCTGGATCAGATAAATCATTGCTTATTCAATGTTTATAAGACATAGGATAGTGTTGTGTAAAt                                               >  2:464913/4‑134 (MQ=255)
                                                                                          tGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTAGCGCAGGTAAATATTTGGAAAAACAATATTTAGAAAACATAAGATATTTTTGTGTAGATAATc                                           >  1:2836928/1‑134 (MQ=255)
                                                                                                 ataatGGATAATAATGAAAAAGAAAAGAGTAAAGTTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGATAAATATTTGGATATTAAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTaccacc                                    >  1:1378123/1‑134 (MQ=255)
                                                                                                  taatTGATAATAATAATAAAAAAAAAAATAAAATTTTATTATTATTTTTAACATTTTTATTTGGCGCAGATAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTAccacca                                   <  1:1333983/134‑1 (MQ=255)
                                                                                                  taatGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTAccacca                                   >  1:243778/1‑134 (MQ=255)
                                                                                                     tGGATAATAATGAAAAAGAAAAAAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAgtg                                <  1:2187366/134‑1 (MQ=255)
                                                                                                      ggATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAgtat                               >  2:1649414/1‑132 (MQ=255)
                                                                                                       gATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGtt                              >  1:1924241/1‑134 (MQ=255)
                                                                                                             aatGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGcc                        <  1:2402640/134‑1 (MQ=255)
                                                                                                                   aaagaaaaaAGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAAtt                  <  1:417378/134‑1 (MQ=255)
                                                                                                                     agaaaaaAGTAAAAGTTAACTATTAGATGTAACAGGTTTATCTGACGCAGATAAAACTTGGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAATGTTATTGCCTAAATTTg                >  2:218841/1‑134 (MQ=255)
                                                                                                                      gaaaaaAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCAACAGTGTTATTGCCTAAATTTGt               >  2:1513112/1‑134 (MQ=255)
                                                                                                                      gaaaaaAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGACGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGt               >  2:58199/1‑134 (MQ=255)
                                                                                                                       aaaaaaGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTa              >  1:599274/1‑134 (MQ=255)
                                                                                                                       aaaaaaGTAAAAGTTAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTa              >  2:24058/1‑134 (MQ=255)
                                                                                                                        aaaaaGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTag             >  2:1298965/1‑134 (MQ=255)
                                                                                                                         aaaagtaaaagtGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTaga            >  2:1856621/1‑134 (MQ=255)
                                                                                                                          aaagtaaaagtGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTagag           >  1:1781104/1‑134 (MQ=255)
                                                                                                                          aaagtaaaagtGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACAAGGGATATTTTTGTGTAGATAATCTAACACCAGAGCTATAGACTAAATTTGTagag           >  1:382390/1‑134 (MQ=255)
                                                                                                                              taatagtGAACTATTATTTGTAACAGGGTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGa       <  2:2660514/134‑1 (MQ=255)
                                                                                                                              taaaagtGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGAGAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGa       <  1:2269651/134‑1 (MQ=255)
                                                                                                                              gaaaagtGAACGATTAGTTGTAACAGGTTTATGTGGCGCAGGTAAAGCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGa       <  2:252432/133‑1 (MQ=255)
                                                                                                                                  agtGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCCATGTTTAGCAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTCCCTAAATTTGTCGCGTTTCTGGa   >  1:1011817/1‑134 (MQ=255)
                                                                                                                                   ttGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGATGGaa  <  1:1605335/133‑1 (MQ=255)
                                                                                                                                   gtGAATTATTTGTTGTAAGAGGTTTATCTGGTGTAGGTAAATCTTTGGTTATTCAATGTTGAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGATGGaa  <  1:321115/134‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                   
TCTAGACATAGTTGGAAATAAATATAAAACATCGTTGCTTAATTTTGTCATAGAACATTTAAATTAACATCATGAAATTCGTTTTGGCGGTGAAAAAATAATGGATAATAATGAAAAAGAAAAAAGTAAAAGTGAACTATTAGTTGTAACAGGTTTATCTGGCGCAGGTAAATCTTTGGTTATTCAATGTTTAGAAGACATGGGATATTTTTGTGTAGATAATCTACCACCAGTGTTATTGCCTAAATTTGTAGAGTTGATGGAA  >  USA300TCH1516_ALE/848775‑849039

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: