BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	USA300TCH1516_ALE	1,744,607	T→C	34.3%	D22G (GAT→GGT)	apt ←	Adenine phosphoribosyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	USA300TCH1516_ALE	1,744,607	0	T	C	34.3%	224.8 / 184.3	251	D22G (GAT→GGT)	apt	Adenine phosphoribosyltransferase
Reads supporting (aligned to +/- strand): ref base T (91/73); new base C (36/50); total (128/123)
Fisher's exact test for biased strand distribution p-value = 4.62e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 262 total aligned reads displayed.

ACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTG  >  USA300TCH1516_ALE/1744475‑1744740

    gACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTCCATAGCCATATGCTTCACCATTATACATAATTATAGTAATACCCTTg                                                                                                                                  >  1:2746566/1‑134 (MQ=255)

         ccAATGATAAAGCCACGCGCTTCAGTTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACt                                                                                                                             <  1:1244257/134‑1 (MQ=255)

          cAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTa                                                                                                                            <  1:273863/134‑1 (MQ=255)

           aaTGATAAAGCCACGCGCTTCAGGTCATACAACGATATCAACATCTCAGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATATCCATATGCTTCACCATTATCAATAATTGTAGTAATATCCTTGAAACTaa                                                                                                                           >  2:2291239/1‑134 (MQ=255)

             tGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGATACTTaca                                                                                                                         >  1:2503119/1‑134 (MQ=255)

             tGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGCTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAaca                                                                                                                         >  2:2674820/1‑134 (MQ=255)

             tGATAAAGCCACGCGCTTCAGGTACTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGCTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAaca                                                                                                                         >  2:75974/1‑134 (MQ=255)

               aTAAAGCGACGCGCTTCAGGTTCTACAACGATATCAACATCTTTGTCTTTTGCTTATTCTATAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATTCATAATTGTAGTAATATCCTTGAAACTAACAcc                                                                                                                       <  2:847655/134‑1 (MQ=255)

                tAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCt                                                                                                                      <  1:2643152/134‑1 (MQ=255)

                 aaaGCCACGCGCTTCCGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACATGTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTg                                                                                                                     <  1:1532488/134‑1 (MQ=255)

                     ccACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATATCCATATTCTTCACAAAAAACAAAAATAGTAGTAAAACTCTTGAAACTAACACCTGtttt                                                                                                                 >  1:712119/1‑134 (MQ=255)

                      cactcgcTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATTGCCTTATGCTTCACCATTATCAATAATTATAGTAATATACTTGAAACTAACACCTGGTTTc                                                                                                                >  1:142938/5‑134 (MQ=255)

                          cgcTTGAGGTCCTACATCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGcc                                                                                                            <  2:67605/134‑1 (MQ=255)

                           gcTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATTGACATAGCCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACTCTTGGtttagacaa                                                                                                           >  1:670116/1‑128 (MQ=255)

                              tCAGTTCCTACAACGATATCCACATCTCTGTTTTTTTCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATc                                                                                                        <  1:1013041/134‑1 (MQ=255)

                              tCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACTCCTGGTTTCGGCCAATc                                                                                                        <  2:1067612/134‑1 (MQ=255)

                              tCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATc                                                                                                        >  1:2029701/1‑134 (MQ=255)

                               cAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGTCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCt                                                                                                       <  1:2177071/134‑1 (MQ=255)

                               cAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCt                                                                                                       <  1:1470627/134‑1 (MQ=255)

                               cAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCt                                                                                                       <  1:2619853/134‑1 (MQ=255)

                                aGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTCTAGTAATATCCTTGAAACTAACACCTGATTTCAGCCAATCtt                                                                                                      >  1:2432436/1‑134 (MQ=255)

                                    ccTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCTATATGCTTCACCATTATCCATAATTGTAGGAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAc                                                                                                  <  1:10402/134‑1 (MQ=255)

                                     cTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACt                                                                                                 >  1:1593423/1‑134 (MQ=255)

                                     cTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACt                                                                                                 >  2:2418116/1‑134 (MQ=255)

                                          aCGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTCGTTTCGGCCAATCTTGACCTTCTGa                                                                                            >  2:128819/1‑134 (MQ=255)

                                           cGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGAt                                                                                           >  1:429494/1‑134 (MQ=255)

                                           cGATATCAACATCTCTGTCTTTTCCGTTTTTTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGAt                                                                                           <  1:1157875/134‑1 (MQ=255)

                                           cGATATCAACATCTCTGTCTTTCGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGAt                                                                                           <  2:2403645/134‑1 (MQ=255)

                                            gATATCAACATCTCTGTCTTTTGCGTATTCTACACTTTTATCTACTGCATAGCCATATGCTTCACCATTATACAAAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATGTTGAACTTCTGATa                                                                                          >  1:637234/1‑134 (MQ=255)

                                              tatCAACATGTCCGTCTTTGTCGTATTCTACGATTTTATCTGCTGCATAGACATATGCTTCTCCATTATCCATAATTGTAGTAAGATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACg                                                                                        <  2:591904/134‑1 (MQ=255)

                                              tatCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACg                                                                                        <  2:1842813/134‑1 (MQ=255)

                                               ttcgaCATCTCTGTCTTTTGCGTATTCTACTATTTTATCTTTTGCATAGCCATATGCTTCACCATTATCTATAATTGTAGTAATACCCTTGAAACTAACAGCTGGTTTCGGCCAATCTTGAACTTCTGATACGt                                                                                       <  2:514194/130‑1 (MQ=255)

                                               atCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAAATTCTGATACGt                                                                                       >  1:1733690/1‑134 (MQ=255)

                                                tCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATGTTGAACTTCTGATACGTa                                                                                      >  1:1458247/1‑134 (MQ=255)

                                                tCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTa                                                                                      >  1:309531/1‑134 (MQ=255)

                                                    cATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTATCGGCCAATCTTGAACTTCTGATACCTATTGc                                                                                  >  2:135316/1‑134 (MQ=255)

                                                     aTCTCTGTCTTTTGCGTATTCTACAATTTTATTTGTTGCATAGCCATATGCTTCACCATTATCCACAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCt                                                                                 <  1:2055231/134‑1 (MQ=255)

                                                      tctcTGTCTTTTTCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACACTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCtt                                                                                <  1:953654/134‑1 (MQ=255)

                                                      tctcTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATTCTTCACCATTATCCATAATTGTAATAATATAATTGACAGATAGATCTGGATTAGGCCAATCTTGAACTTCTTATACGTATTGCtt                                                                                >  1:105585/1‑134 (MQ=255)

                                                      tctcTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGTCAATCTTGAACTTCTGATACGTATTGCtt                                                                                <  2:2165177/134‑1 (MQ=255)

                                                      tctcTGTCTTTTGCGTATCCTACAATTTTATCTGTTGCATAGCCATATGCTTCACGATTGTCCCTTATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCTTCCATTCCTGAACTTCTGATACGTATTGCtt                                                                                <  2:2185016/134‑1 (MQ=255)

                                                       ttctttcttTTTCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCttt                                                                               <  2:96130/133‑1 (MQ=255)

                                                       ctctGTCTTTTGCGTATTCTACAATTTTATCTTTTGCATAGCCATATGCTTCAACATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCttt                                                                               <  1:561603/134‑1 (MQ=255)

                                                       ctctGTCTTTTGCGTATTCTACAATTTTATCTGTTACATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCttt                                                                               <  1:2165427/134‑1 (MQ=255)

                                                        tctGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACGATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTGTCGGCCAATATTGAGCTTATGATACGTCTTACTTTa                                                                              >  2:1253789/1‑134 (MQ=255)

                                                                   cGTATTCTACAATTGTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAat                                                                   <  2:1446335/134‑1 (MQ=255)

                                                                    gTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGGTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAata                                                                  <  1:2310073/134‑1 (MQ=255)

                                                                          tACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTtcct                                                            >  2:2686369/1‑134 (MQ=255)

                                                                           aCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTtcctc                                                           >  2:2641577/1‑134 (MQ=255)

                                                                           aCAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATGGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTtcctc                                                           >  2:2341576/1‑134 (MQ=255)

                                                                               tttgATCTGTTGCATAGCCAGATGCTTCACGATTATCCATAATTGTAGTAATATGCTTGAATCTAACACGTGGTTTCGTCCAATCTTGAACTTCTGATTCGTGTGATTATGGCTCTATTGATATTTCCTCCTaa                                                       <  2:209771/134‑1 (MQ=255)

                                                                                tttATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTaaa                                                      >  1:802985/1‑134 (MQ=255)

                                                                                tttATCTGTTGCATAGCAATATGCTTCACCATTATCCATACAAGACGAAAAAGGCTTACAACTAGGACCTGGTTTCGGCCAATCTTGAAATTCTGATACGTAATGCTTTAAATCCATTAATATTCCCCACTaaa                                                      >  2:1306127/1‑134 (MQ=255)

                                                                                    tCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGc                                                  >  1:2295201/1‑134 (MQ=255)

                                                                                    tCTGTTGAATAGGCTGATGCTTCATCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCAAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGc                                                  <  2:309261/134‑1 (MQ=255)

                                                                                     cTGTTGCATAGCCATATGCTTCACCATTATCCATAACTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTTCt                                                 >  2:43174/1‑134 (MQ=255)

                                                                                      tGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTTCTTTAAATCCATTAATATTTCCTCCTAAATTGCTc                                                >  2:1956476/1‑134 (MQ=255)

                                                                                            atacgcATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACa                                          >  2:1777793/6‑134 (MQ=255)

                                                                                              aGCCATATGCTTCACTATTATCCATAAGTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAAt                                        <  2:2842653/134‑1 (MQ=255)

                                                                                               gCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGATTTAAATCCATTAATATTTCATCCTAAATTGCTCACGACAAtt                                       >  1:1448667/1‑134 (MQ=255)

                                                                                                 cATATGCTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTAGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCAATAATATTTCCTCCTAAATTGCTCACGACAATtgt                                     >  2:507872/1‑134 (MQ=255)

                                                                                                  atatGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATtgtg                                    <  2:2545856/134‑1 (MQ=255)

                                                                                                    gtGCTTGACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGAc                                  >  2:1196052/2‑134 (MQ=255)

                                                                                                       cTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACttt                               <  2:540240/134‑1 (MQ=255)

                                                                                                       cTTCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACttt                               >  2:2446412/1‑134 (MQ=255)

                                                                                                         tCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTAt                             >  1:2508353/1‑134 (MQ=255)

                                                                                                         tCACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTAt                             <  2:1033376/134‑1 (MQ=255)

                                                                                                          cACCATTATCCATAATTGTCGTAATATCCTTGAAACTAACACCTGGTTTCGGCCACTCTTGAACTTCTGATAAATATTACTTTAAAAACATTAATATCTCCTCCTAAATTTCTCACGCCCATTGTGACTTTATc                            >  1:747920/1‑134 (MQ=255)

                                                                                                          cACCATTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATc                            <  2:369667/134‑1 (MQ=255)

                                                                                                           aCCATTATCCTTTATGGTAGTATTATCCCAGTAACTAACACCTGGTTTCGCCCAATCTTTAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATcc                           <  2:2766122/134‑1 (MQ=255)

                                                                                                              aTTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGCACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTACCTTGTTCACGACAATTTTGACTTTCCCCAAt                        >  1:238415/1‑134 (MQ=255)

                                                                                                              aTTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTTACTTTAttaaat                        >  1:1297558/1‑129 (MQ=255)

                                                                                                              aTTATCCATAATTGTAGTAATACCCTTGAAACTAACACCTGATTTCGGCCAAACTTGAACTACTGATCCGTATTGCTTTAAATCCATTAACATTTCCTCCTAAATTGCTCACGACACTTGTGACTTTATCCAAt                        >  2:621037/1‑134 (MQ=255)

                                                                                                               ttATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGCATGTCATTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAtt                       >  2:1740386/1‑134 (MQ=255)

                                                                                                               ttATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTCATACGTATTGCTTTAAAACCATTAATATATCATCCTAAATTGCTCACGACAATTGTGACTTTATCCAAtt                       >  1:486872/1‑134 (MQ=255)

                                                                                                                tgttgaTAATTGTAGTTATATCTTTGAAACTAACTTCTGGTTTCGGTCAATCTTGTACTTCTGATACTTATTGCTTTAAATCCATTAATATTTCCTCCTATATTGCTCACGACAATTGTGACTTTTTCCTAttt                      <  2:2006060/129‑1 (MQ=255)

                                                                                                                tATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTTATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAttt                      <  1:2391527/134‑1 (MQ=255)

                                                                                                                   cgaTAATTGTAGTAAGACTCTTGAAACTAACAGCTGGGTTCGGCCTATCTTGAACTTCTGATACGTATTGCTGTAAATGCAGTAATATTTCCTCCTAAATTGCTCACGACAATTGTTACTTTATCCAAtttttt                   <  2:1948690/132‑1 (MQ=255)

                                                                                                                   ccATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTCATATTTCCTCCTACACTGCCCACCACAATTGTGACTTTATCCAAtttttt                   >  2:2034983/1‑134 (MQ=255)

                                                                                                                   ccATAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAAtttttt                   <  1:1194013/134‑1 (MQ=255)

                                                                                                                      tAATTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTAtt                <  1:2680446/134‑1 (MQ=255)

                                                                                                                        atggtagtaATATCCTTTGAATTATCACCTGGTTTCGGCCAATCGTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTGCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTc              <  2:1680768/131‑1 (MQ=255)

                                                                                                                        aTTGTAGTAATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTc              <  2:1850558/134‑1 (MQ=255)

                                                                                                                          tGTAGTAATATCCGTGAAACTAACACCAGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTg            <  1:2206428/134‑1 (MQ=255)

                                                                                                                           gtagtaATATACTTTAACCTATCACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTGCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGa           <  2:405775/134‑1 (MQ=255)

                                                                                                                           gtagtaATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGa           <  1:2153732/134‑1 (MQ=255)

                                                                                                                            tagtaATATCCTTGAAACTAACACCTGGTGTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaa          <  2:2288674/134‑1 (MQ=255)

                                                                                                                            tagtaATACTCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaa          <  2:1601301/134‑1 (MQ=255)

                                                                                                                             agttATATCCTTTAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTGCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaa         <  1:1306127/134‑1 (MQ=255)

                                                                                                                             agtaATATCCTTGAAACTAGCTCCTGGTTTCGGCCATTCTTGTACTTCTGATACGTATTGCTTTAAATTCATTAATATTTTCTCCTAAATTGCTCACAACAATTGTGACTTTATCCATTTTTTTATTTCTGaaa         <  2:2061654/134‑1 (MQ=255)

                                                                                                                             agtaATATCCTTGAAACTAACACCTGGTTTCGTCCAATCTTGAACTTCTGCTACGTATTGCTTTAAATCCATTAATATTTCATCCTAAATTACTACAGACCATTGTGACTTTATCCTAATTTTTATTTTTGaca         >  2:68322/1‑132 (MQ=255)

                                                                                                                             agtaATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTGTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaa         >  2:1476644/1‑134 (MQ=255)

                                                                                                                             agtaATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaa         >  1:1921924/1‑134 (MQ=255)

                                                                                                                             agtaATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATAAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaa         >  1:1713073/1‑134 (MQ=255)

                                                                                                                               taATACCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGaaaaa       <  2:1603065/134‑1 (MQ=255)

                                                                                                                                 atacCCTTGAAACTAACACCTGGTTTCGGTCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATc     <  1:1872711/134‑1 (MQ=255)

                                                                                                                                 atacCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATc     <  1:1081589/134‑1 (MQ=255)

                                                                                                                                  tatCCTTGAAACTAACAACTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTCATTTTATCCAATTTTTAATTTCTGAAAAAttt    >  2:1693594/1‑132 (MQ=255)

                                                                                                                                    tCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTg  >  2:802810/1‑134 (MQ=255)

                                                                                                                                    tCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGAAAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTg  >  1:1990362/1‑134 (MQ=255)

ACAGGACAGCCAATGATAAAGCCACGCGCTTCAGGTCCTACAACGATATCAACATCTCTGTCTTTTGCGTATTCTACAATTTTATCTGTTGCATAGCCATATGCTTCACCATTATCCATAATTGTAGTAATATCCTTGAAACTAACACCTGGTTTCGGCCAATCTTGAACTTCTGATACGTATTGCTTTAAATCCATTAATATTTCCTCCTAAATTGCTCACGACAATTGTGACTTTATCCAATTTTTTATTTCTGAAAAATCTTG  >  USA300TCH1516_ALE/1744475‑1744740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`