| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | USA300TCH1516_ALE | 2,279,705 | G→A | 100% | A943V (GCA→GTA) | ebh_3 ← | Extracellular matrix‑binding protein ebh |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | USA300TCH1516_ALE | 2,279,705 | 0 | G | A | 100.0% | 927.0 / ‑6.5 | 336 | A943V (GCA→GTA) | ebh_3 | Extracellular matrix‑binding protein ebh |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (159/170); minor base T (1/3); total (161/175) | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
Only 100 of 336 total aligned reads displayed.
GTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAA > USA300TCH1516_ALE/2279575‑2279833 | gTTATTAGTTTATTCTACTTCTTGTTTACTCGTTACATCGTTATTCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg < 2:1632695/134‑1 (MQ=255)gTTATTAGTTGATTCTACTTCTGTTTTACTAGTTACATCGTTATCCTTTGTTGGATTGTTTGTTGATGCATCTACACTAGAATTGTGATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACtg < 1:250899/134‑1 (MQ=255)gTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATAATCAGTTGCTGATGTTACtg > 1:2002813/1‑134 (MQ=255) taGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATCGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTgttgtt > 1:1581909/1‑134 (MQ=255) aGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGttt < 1:832388/134‑1 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACTTCGTTATCCATTGTCGGACTGTTTGTTGATGTATTTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc < 1:967284/134‑1 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGCTGTTACTGTTGTTTc > 2:1867938/1‑134 (MQ=255) gTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGAGGTCTCTTATGAACTTCATCAGTTGCTGATGTTACTGTTGTTTc > 1:1222870/1‑134 (MQ=255) cttGATTCTTCTTCTGTTTTACTAGTTATATCGTTATCCATTGGCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTc < 1:2373708/133‑1 (MQ=255) aTTTTAGTTCTGGTTTACTATTTACATCGTTTTCCTTTGTCGTACTGTTTGTTTGTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCt < 2:440159/134‑1 (MQ=255) aTTCTACTTCTGGTTTACTAGTTACATAGTTACCCATTGTCGGATTGTTTTTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTCTATCATTTCCATCTTCAGTTGCTGATTTTACTGttttttgctgt > 2:2865965/1‑129 (MQ=255) tCTACTTCTGGTTTACTAGTTACATCGTTTTCCATTGTCGGACTGTTTTTTTATGCATTTACATTTTATTTTTTATTAGTTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt < 1:1114122/134‑1 (MQ=255) tCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt > 1:1751024/1‑134 (MQ=255) tCTACTTCTGGTTTACTAGTTACATAGTTATGCGATCTCTCACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGt < 1:1955699/134‑1 (MQ=255) cTACTTCTGGTTTATTAGTTACATCGTTATTCATTGTCGGACTGTTTGTTGATGGATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGtt < 1:2766002/134‑1 (MQ=255) tACTTCTTGTTTACTATTTACATCGTTATCCATTGTCGTACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 1:1310646/134‑1 (MQ=255) tACTTCTGGTTTGCTAGTTACGTTGTTATTCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAAGTGTTATTAGCGTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 2:2439298/134‑1 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTTGAATTGTTATTATCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 1:1497732/134‑1 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTTTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 1:2437470/134‑1 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATTTTACTGTTGTTTCACCTGTTg > 2:1686405/1‑134 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 1:1811878/134‑1 (MQ=255) tACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTg < 2:1491295/134‑1 (MQ=255) aCTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGc > 2:1353293/1‑134 (MQ=255) cTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGTCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGcc < 1:1639539/134‑1 (MQ=255) ttCTGGTTTACTAGTTACATCGTTATCCATTGTCGTACTGTTTGTTTATGCATCTACACTAGAATTATTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCg < 1:39445/134‑1 (MQ=255) gTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa < 2:513138/134‑1 (MQ=255) gTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCa < 1:2450133/134‑1 (MQ=255) tttACTAGTTACATCGTTATCCATTGTCGGATTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTGACCTGTTGCCGCATCAc < 2:1047398/134‑1 (MQ=255) tttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCAc < 1:1410402/134‑1 (MQ=255) ttACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACATTAGAATTGTTATTAGCTGGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACt < 1:891045/134‑1 (MQ=255) tACTAGTTACATCGTTATCCATTGTCGGACTTTTTGTTGATGTATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACta < 1:1024103/134‑1 (MQ=255) tACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAACTTAAGGATAATCATTTGAAACATCAGTTGCTGATGTTACTGTTGTTTAACCTGTTGCCGCATCACta > 1:58568/1‑134 (MQ=255) aCTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACtat > 1:1429290/1‑134 (MQ=255) ttACATCGTTATTCATTGTCGGACTGTTTGTTGTTGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt < 2:31045/134‑1 (MQ=255) gtACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTTCACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTAttt < 1:1483331/133‑1 (MQ=255) aTCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtg < 2:756122/134‑1 (MQ=255) tCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTCTTTCACCTGTTGCCGCATCACTATTATTTGgtgt > 2:1258989/1‑134 (MQ=255) tCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTACGGTTTATCATTTGCATCATAAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGgtgt > 1:1367683/1‑134 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATTTTACTGTTGTTTCACCTGTTGCAGCATCACTATTATTTGGtgtt > 2:1510175/1‑134 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTACTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgtt > 1:214291/1‑134 (MQ=255) cGTTATCCATTGTCGGACTGTTTGTCGATGCTTCTACACTAGAATTGTTATTCGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTCTTTCACCTGTTGCCGCATCACTATCATTTGGtgtt > 1:433390/1‑134 (MQ=255) gTTATCCATTGTCGGACAGTTTGTATATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTACCGCATCACTATTATTTGGtgttg > 1:2128956/1‑134 (MQ=255) ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGtgttgt > 1:749528/1‑134 (MQ=255) ttATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACATGTTGCCGCATCACTATTATTTGGtgttgt > 1:340062/1‑134 (MQ=255) aTCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTCTTTCGTGTTGTCg > 2:221065/1‑134 (MQ=255) tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGTTTAAGTGGGAACTAATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg > 1:2852031/1‑134 (MQ=255) tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg > 2:2318958/1‑134 (MQ=255) tCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCgg > 1:270370/1‑134 (MQ=255) ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga > 2:2098527/1‑134 (MQ=255) ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga > 2:1897437/1‑134 (MQ=255) ccATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTATTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGga > 1:989166/1‑134 (MQ=255) cATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGgag > 2:2602034/1‑134 (MQ=255) ttGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCCTCACTCTTATTTGGCTTGGTCgaagaa > 2:798942/1‑134 (MQ=255) gTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACCATTATTTGGTGTTGTGGGAGAAGc > 2:2548505/1‑134 (MQ=255) cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGt < 2:549207/134‑1 (MQ=255) cGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGACTCATCAGTTACTGATGTTAATATTGTTTCACATGTTGACGCATCAATAAAATTTGAAGTTATCGCAGAAGCga > 1:1161843/1‑133 (MQ=255) aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTg > 2:2757103/1‑134 (MQ=255) aCTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAATTGATGATGTTACTGTTGTTTCAACTGTAGCCACGTAACTATTATTTGGTGTTGTCGGAGAAGAGTCtc > 1:2233360/1‑133 (MQ=255) cTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTTCATCATCAGTTGCTGATGTTACTATTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGc > 1:1759658/1‑134 (MQ=255) tgtttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATAACTATTATTTGGTGTTGTCGGATAAGCGTCTGCt > 1:234920/1‑134 (MQ=255) tttgttGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCAACTGTTGCAGCATCACTATTATTAGGTGTTGTCGGAGAAGCGTCTGCttt > 1:637672/1‑134 (MQ=255) ttgttgATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTg > 1:2811089/1‑134 (MQ=255) gATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGGTGAGTTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGTCAtt > 2:2129623/1‑134 (MQ=255) gCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCCTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTCGTCGGAGCAGCGTCTGCTTTGCCATTacc > 2:624486/1‑132 (MQ=255) tCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgt > 2:410571/1‑134 (MQ=255) acacTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgt < 1:1867938/134‑1 (MQ=255) cattAGAATTTTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTgtcgtc < 2:1818121/131‑1 (MQ=255) acTAGGATTGTTACTAGCTTGCTGTTTATCATCTTCATCATCATTTGTTGATTTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTTTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGtct < 2:380460/134‑1 (MQ=255) aGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAg > 1:1467425/1‑134 (MQ=255) gtaTTGTTATTATCTTGTGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGa < 2:2075468/132‑1 (MQ=255) gAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTATTGTTTCACATGTTGCCGCATCACTATCATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAgc > 2:1170431/1‑133 (MQ=255) gTTATTAGCTTGCTGTTTATCATTTGCATCATCAGTTGTTGATGTTACTGTTGTTTGACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 1:1171143/134‑1 (MQ=255) gTTATTAGCTTGCGGTTTATGATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGt < 2:226833/134‑1 (MQ=255) gTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCTTATGCTTTACCATTAGCTGTCGTTTCAGATActa > 1:1131496/1‑132 (MQ=255) tattaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTa > 2:933513/1‑134 (MQ=255) tattaGCTTGCGGTTAATCCTTATAGGCTTTAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTa > 2:1606378/1‑134 (MQ=255) attaGCTTGCGGTTTATCATTTGCATCATCAGTTGTTGATGTTACTGTTTTTTCACCTGTTGTCGCATCACTATTATTTGGTGTTGTCGGAGAAGTGTCTTCGTTGCCATTAGCTGTCGTCTCAGATACGTTAg < 1:807905/134‑1 (MQ=255) attaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGGTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAg < 2:172739/134‑1 (MQ=255) ttaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg > 2:1297554/1‑134 (MQ=255) ttaGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAgg > 1:2148742/1‑134 (MQ=255) taGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCAGAGAAACGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGt > 1:1911635/1‑134 (MQ=255) tGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTcc < 1:2624231/134‑1 (MQ=255) cGGTTTATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCCTAACTCTTATTTGGAGTTGTAGGAGAAGCGTATGCTTTGCAATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAg > 1:488139/1‑134 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGTCGCATCTCTATTTTTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCGGTTGTCTCAGATACGTTAGGTTGTCCAGTAttt < 1:2256888/134‑1 (MQ=255) tATCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTCCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGACTGCTTTGCCATTACCTGTCGTCTCAGATACGTAACGTTGTCCACTAttt > 2:722181/1‑134 (MQ=255) aTCATTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTAtttt > 1:1614398/1‑134 (MQ=255) aTTTGCATCATCAGTTGCTGATGTTACTGTTGTTTCATCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCATGTCATTTAGGTTAGGTTGTCCAGTATTTTCTg < 2:1754087/134‑1 (MQ=255) ttGCATCGTCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTCTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTATCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGgt > 2:1959676/1‑134 (MQ=255) catcatCAGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGtt < 2:2002813/134‑1 (MQ=255) tcatcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCAACACTATTCTTTGGTGTTGTCGGAGAAGAGTCTGCTTTGCCATTAGCTGTCGTCCAAGATACGTAAGGTTGTGCAGTATTTTCTGGTGTTGc > 1:1269817/1‑134 (MQ=255) tcatcaGTTGCTGATGTTACTGTTGTGTCACCTGTTGCCGCATCACTATGATTTGGTGTTGTCTGAGGAGCGTCTCACTCGCCCCTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGc < 2:1425672/134‑1 (MQ=255) catcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCa < 1:773499/134‑1 (MQ=255) gtcaGTTGCTGATGTTAGGGTTGTTGCATCTGTTGCTGCATCAGTATTATTTGGTGTTGTCGTAGAAGCTTCGTCTTTGGCAGTAGCTGTCGTTTCAGATACGTTAGGTTGTCTAGTATTTTCTGGTGTTGCAt < 2:1940949/133‑1 (MQ=255) atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTTTTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAt > 1:165918/1‑134 (MQ=255) atcaGTTGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCAt > 2:1652842/1‑134 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCAt < 2:2376822/134‑1 (MQ=255) tGCTGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCATCTGCTTTGCCATTAACTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTATGGTGTTGCATTAGCAt > 1:727626/1‑134 (MQ=255) tGATGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTg > 1:2195073/1‑134 (MQ=255) aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa < 1:2318958/134‑1 (MQ=255) aTGTTACTGTTGTTTCACCTGTTGCCGCATCACTATTATATGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGaa > 2:905894/1‑134 (MQ=255) | GTTATTAGTTGATTCTACTTCTGGTTTACTAGTTACATCGTTATCCATTGTCGGACTGTTTGTTGATGCATCTACACTAGAATTGTTATTAGCTTGCGGTTTATCATTTGCATCATCAGTTGCTGATGTTGCTGTTGTTTCACCTGTTGCCGCATCACTATTATTTGGTGTTGTCGGAGAAGCGTCTGCTTTGCCATTAGCTGTCGTCTCAGATACGTTAGGTTGTCCAGTATTTTCTGGTGTTGCATTAGCATTTGAA > USA300TCH1516_ALE/2279575‑2279833 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |